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Hi sir i am work on chip from tissue problem is that after sonication we get two fraction one is pellet and another is supernatant then confusion is that which portion contain DNA fragment.Thanks.
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Hello sir,i am also trying to do chip on tissue samples , initially the amount of sample taken by me was 70 mg. after sonication() step i took around 100microlitre of this sonicated product and followed the phenol-CHCL3(ph 7.6-8 which is used to isolate dna ) procedure to isolate the dna. the O.D obtained was 45ng/microlitre and 260/280 ratio was 2.1(i know this ratio represents RNA ) i also checked my product on 2 % agarose gel but found no band in my products.so what does it conclude...where my dna has gone....has it been degraded... or was present in the pellet of cells that i have thrown as waste after centrifugation... or was present in a different layer while dna purification that was removed by me... if i can isolate rna(which gets easily degraded due to RNase action ) then how could i not get dna as dna is more stable than rna and as all hygienic conditions were maintained .needs your suggestion to this situation.thanks for reading with all your patience ....
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