神经科学

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现刊

往期刊物

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Application of Electrical Stimulation to Enhance Axon Regeneration Following Peripheral Nerve Injury

电刺激增强周围神经损伤后的轴突再生的应用

SW Supriya S. Wariyar PW Patricia J. Ward*

0 Q&A 226 Views Oct 5, 2023

Enhancing axon regeneration is a major focus of peripheral nerve injury research. Although peripheral axons possess a limited ability to regenerate, their functional recovery is very poor. Various activity-based therapies like exercise, optical stimulation, and electrical stimulation as well as pharmacologic treatments can enhance spontaneous axon regeneration. In this protocol, we use a custom-built cuff to electrically stimulate the whole sciatic nerve for an hour prior to transection and repair. We used a Thy-1-YFP-H mouse to visualize regenerating axon profiles. We compared the regeneration of axons from nerves that were electrically stimulated to nerves that were not stimulated (untreated). Electrically stimulated nerves had longer axon growth than the untreated nerves. We detail how variations of this method can be used to measure acute axon growth.

Denervation of Mouse Lower Hind Limb by Sciatic and Femoral Nerve Transection

通过坐骨和股神经横切对小鼠下后肢进行去神经试验

YR Yuval Rinkevich* DM Daniel T. Montoro EM Ethan Muhonen DL David Lo MH Masakazu Hasegawa CM Clement D. Marshall GW Graham G. Walmsley

Andrew Connolly IW Irving L. Weissman ML Michael T. Longaker*

0 Q&A 15738 Views Jul 5, 2016

The requirement and influence of the peripheral nervous system on tissue replacement in mammalian appendages remain largely undefined. Reports from salamander models of appendage regeneration (Singer, 1952; Singer, 1947; Kumar et al., 2007), and of human clinical skin and nail problems associated with spinal cord injury patients (Stover et al., 1994) suggest that appendage regeneration may have an important nerve component. To explore this question, we have generated hind limb tissues devoid of nerve supply. This protocol, combined with multi-color ‘Rainbow’ reporter mouse lines permits single cell clonal analysis and genetic lineage tracing studies in the absence of nerve supply (Rinkevich et al., 2014), exposing nerve requirements on cellular replacement and differentiation during tissue growth, maintenance, and regeneration.