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Vicente Bernal


PhD in Biochemistry in the University of Murcia (Spain) in 2007

Current position

Dept. Biochemistry and Molecular Biology B and Immunology. Faculty of Chemistry. University of Murcia, Spain. His research activity has always been related with Metabolic Engineering and Systems Biology of microorganisms and animal cells. More particularly, his present research interests are focused on transcriptional and post-translational regulational mechanisms of metabolic control and their application in the biotechnological and biomedical research fields


  1. Renilla, S., Bernal, V., Fuhrer, T., Castano-Cerezo, S., Pastor, J. M., Iborra, J. L., Sauer, U. and Canovas, M. (2012). Acetate scavenging activity in Escherichia coli: interplay of acetyl-CoA synthetase and the PEP-glyoxylate cycle in chemostat cultures. Appl Microbiol Biotechnol 93(5): 2109-2124.
  2. Castano-Cerezo, S., Bernal, V., Blanco-Catala, J., Iborra, J. L. and Canovas, M. (2011). cAMP-CRP co-ordinates the expression of the protein acetylation pathway with central metabolism in Escherichia coli. Mol Microbiol 82(5): 1110-1128.
  3. Carinhas, N., Bernal, V., Teixeira, A. P., Carrondo, M. J., Alves, P. M. and Oliveira, R. (2011). Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products. BMC Syst Biol 5: 34.
  4. Bernal, V., Monteiro, F., Carinhas, N., Ambrosio, R. and Alves, P. M. (2010). An integrated analysis of enzyme activities, cofactor pools and metabolic fluxes in baculovirus-infected Spodoptera frugiperda Sf9 cells. J Biotechnol 150(3): 332-342.
  5. Pastor, J. M., Salvador, M., Argandona, M., Bernal, V., Reina-Bueno, M., Csonka, L. N., Iborra, J. L., Vargas, C., Nieto, J. J. and Canovas, M. (2010). Ectoines in cell stress protection: uses and biotechnological production. Biotechnol Adv 28(6): 782-801.
  6. Metabolic adaptation of Escherichia coli to long-term exposure to salt stress. Process Biochemistry, vol. 45, no. 9, pp. 1459-1467, 2010.
  7. Amaral, A. I., Teixeira, A. P., Martens, S., Bernal, V., Sousa, M. F. and Alves, P. M. (2010). Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging 13C NMR spectroscopy and metabolic flux analysis. J Neurochem 113(3): 735-748.
  8. Carinhas, N., Bernal, V., Monteiro, F., Carrondo, M. J., Oliveira, R. and Alves, P. M. (2010). Improving baculovirus production at high cell density through manipulation of energy metabolism. Metab Eng 12(1): 39-52.
  9. Castaño-Cerezo, S., Pastor, J. M., Renilla, S., Bernal, V., Iborra, J. L. and Cánovas, M. (2009). An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli. Microbial Cell Factories 8(1): 1-19.
  10. Bernal, V., Carinhas, N., Yokomizo, A. Y., Carrondo, M. J. and Alves, P. M. (2009). Cell density effect in the baculovirus-insect cells system: a quantitative analysis of energetic metabolism. Biotechnol Bioeng 104(1): 162-180.
  11. Carinhas, N., Bernal, V., Yokomizo, A. Y., Carrondo, M. J., Oliveira, R. and Alves, P. M. (2009). Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol 81(6): 1041-1049.
  12. Bernal, V., Arense, P., Blatz, V., Mandrand-Berthelot, M. A., Canovas, M. and Iborra, J. L. (2008). Role of betaine:CoA ligase (CaiC) in the activation of betaines and the transfer of coenzyme A in Escherichia coli. J Appl Microbiol 105(1): 42-50.
  13. Bernal, V., Masdemont, B., Arense, P., Canovas, M. and Iborra, J. L. (2007). Redirecting metabolic fluxes through cofactor engineering: Role of CoA-esters pool during L(-)-carnitine production by Escherichia coli. J Biotechnol 132(2): 110-117.
  14. Bernal, V., Sevilla, Á., Cánovas, M. and Iborra, J. L. (2007). Production of L-carnitine by secondary metabolism of bacteria. Microbial Cell Factories 6(1): 31.
  15. Bernal, V., Gonzalez-Veracruz, M., Canovas, M. and Iborra, J. L. (2007). Plasmid maintenance and physiology of a genetically engineered Escherichia coli strain during continuous L-carnitine production. Biotechnol Lett 29(10): 1549-1556.
  16. Role of wet experiment design in data generation: From in vivo to in silico and back. In Silico Biology, vol. 7, no. SUPPL. 2, pp. 3-16, 2007.
  17. Canovas, M., Bernal, V., Sevilla, A., Torroglosa, T. and Iborra, J. L. (2007). Salt stress effects on the central and carnitine metabolisms of Escherichia coli. Biotechnol Bioeng 96(4): 722-737.
  18. Cánovas, M., García, V., Bernal, V., Torroglosa, T. and Iborra, J. L. (2007). Analysis of Escherichia coli cell state by flow cytometry during whole cell catalyzed biotransformation for L-carnitine production. Process Biochemistry 42(1): 25-33.
  19. Canovas, M., Sevilla, A., Bernal, V., Leal, R. and Iborra, J. L. (2006). Role of energetic coenzyme pools in the production of L-carnitine by Escherichia coli. Metab Eng 8(6): 603-618.
  20. Cánovas, M., Bernal, V., González, M., Kleber, H. and Iborra, J. (2005). Factors affecting the biotransformation of trimethylammonium compounds into L-carnitine by Escherichia coli. Biochemical engineering journal 26(2): 145-154.
  21. Canovas, M., Bernal, V., Torroglosa, T., Ramirez, J. L. and Iborra, J. L. (2003). Link between primary and secondary metabolism in the biotransformation of trimethylammonium compounds by escherichia coli. Biotechnol Bioeng 84(6): 686-699.
Protocols by Vicente Bernal
  1. Acetyl-coenzyme A Synthetase (Acs) Assay