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Cheng-Ming Chiang

Education

Ph.D., Department of Biochemistry, University of Rochester, New York, 1991

Current position

Professor, Simmons Comprehensive Cancer Center, Departments of Pharmacology and Biochemistry, UT Southwestern Medical Center at Dallas

Research focus: Mechanisms of transcription and gene regulation in human papillomaviruses; functional regulation of transcription factors by posttranslational modification; functional interplays between DNA tumor virus-encoded oncoproteins and cellular tumor suppressor proteins; chromatin and epigenetic modification of chromosomal and non-chromosomal binding proteins

Publications (since 2005)

  1. Yang, X. J. and Chiang, C. M. (2013). Sumoylation in gene regulation, human diseases and therapeutic actions. F1000 Biology Reports In press
  2. Wu, S. Y., Lee, A. Y., Lai, H. T., Zhang, H. and Chiang, C. M. (2013). Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting. Mol Cell 49(5): 843-857.
  3. Quinlan, E. J., Culleton, S. P., Wu, S. Y., Chiang, C. M. and Androphy, E. J. (2013). Acetylation of conserved lysines in bovine papillomavirus E2 by p300. J Virol 87(3): 1497-1507.
  4. Chiang, C. M. (2012). p53-Aurora A mitotic feedback loop regulates cell cycle progression and genomic stability. Cell Cycle 11(20): 3719-3720.
  5. Loewenstein, P. M., Wu, S. Y., Chiang, C. M. and Green, M. (2012). The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro. Virology 428(1): 70-75.
  6. Rabellino, A., Carter, B., Konstantinidou, G., Wu, S. Y., Rimessi, A., Byers, L. A., Heymach, J. V., Girard, L., Chiang, C. M., Teruya-Feldstein, J. and Scaglioni, P. P. (2012). The SUMO E3-ligase PIAS1 regulates the tumor suppressor PML and its oncogenic counterpart PML-RARA. Cancer Res 72(9): 2275-2284.
  7. Hsu, C. H., Peng, K. L., Jhang, H. C., Lin, C. H., Wu, S. Y., Chiang, C. M., Lee, S. C., Yu, W. C. and Juan, L. J. (2012). The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription. Oncogene 31(18): 2335-2349.
  8. Wang, W. M., Wu, S. Y., Lee, A. Y. and Chiang, C. M. (2011). Binding site specificity and factor redundancy in activator protein-1-driven human papillomavirus chromatin-dependent transcription. J Biol Chem 286(47): 40974-40986.
  9. Cai, D., Lee, A. Y., Chiang, C. M. and Kodadek, T. (2011). Peptoid ligands that bind selectively to phosphoproteins. Bioorg Med Chem Lett 21(17): 4960-4964.
  10. Ponugoti, B., Kim, D. H., Xiao, Z., Smith, Z., Miao, J., Zang, M., Wu, S. Y., Chiang, C. M., Veenstra, T. D. and Kemper, J. K. (2010). SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism. J Biol Chem 285(44): 33959-33970.
  11. Zhou, G., Liu, Y., Wu, S. Y., Tie, F., Lou, H., Chiang, C. M. and Luo, G. (2010). Purification of a novel RECQL5-SWI/SNF-RNAPII super complex. Int J Biochem Mol Biol 1(1): 101-111.
  12. Huang, C. C., Li, Y., Lopez, A. B., Chiang, C. M., Kaufman, R. J., Snider, M. D. and Hatzoglou, M. (2010). Temporal regulation of Cat-1 (cationic amino acid transporter-1) gene transcription during endoplasmic reticulum stress. Biochem J 429(1): 215-224.
  13. Chiang, C. M. (2009). Brd4 engagement from chromatin targeting to transcriptional regulation: selective contact with acetylated histone H3 and H4. F1000 Biol Rep 1: 98.
  14. Kim, Y. C., Wu, S. Y., Lim, H. S., Chiang, C. M. and Kodadek, T. (2009). Non-proteolytic regulation of p53-mediated transcription through destabilization of the activator.promoter complex by the proteasomal ATPases. J Biol Chem 284(50): 34522-34530.
  15. Kemper, J. K., Xiao, Z., Ponugoti, B., Miao, J., Fang, S., Kanamaluru, D., Tsang, S., Wu, S. Y., Chiang, C. M. and Veenstra, T. D. (2009). FXR acetylation is normally dynamically regulated by p300 and SIRT1 but constitutively elevated in metabolic disease states. Cell Metab 10(5): 392-404.
  16. Wu, S. Y. and Chiang, C. M. (2009). p53 sumoylation: mechanistic insights from reconstitution studies. Epigenetics 4(7): 445-451.
  17. Huang, C. C., Chiribau, C. B., Majumder, M., Chiang, C. M., Wek, R. C., Kelm, R. J., Jr., Khalili, K., Snider, M. D. and Hatzoglou, M. (2009). A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur α). J Biol Chem 284(47): 32312-32320.
  18. Yang, J., Kong, X., Martins-Santos, M. E., Aleman, G., Chaco, E., Liu, G. E., Wu, S. Y., Samols, D., Hakimi, P., Chiang, C. M. and Hanson, R. W. (2009). Activation of SIRT1 by resveratrol represses transcription of the gene for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) by deacetylating hepatic nuclear factor 4α. J Biol Chem 284(40): 27042-27053.
  19. Wu, S. Y. and Chiang, C. M. (2009). Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28(9): 1246-1259.
  20. Lee, A. Y. and Chiang, C. M. (2009). Chromatin adaptor Brd4 modulates E2 transcription activity and protein stability. J Biol Chem 284(5): 2778-2786.
  21. Fang, S., Tsang, S., Jones, R., Ponugoti, B., Yoon, H., Wu, S. Y., Chiang, C. M., Willson, T. M. and Kemper, J. K. (2008). The p300 acetylase is critical for ligand-activated farnesoid X receptor (FXR) induction of SHP. J Biol Chem 283(50): 35086-35095.
  22. Wang, W. M., Lee, A. Y. and Chiang, C. M. (2008). One-step affinity tag purification of full-length recombinant human AP-1 complexes from bacterial inclusion bodies using a polycistronic expression system. Protein Expr Purif 59(1): 144-152.
  23. Wu, S. Y. and Chiang, C. M. (2007). The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation. J Biol Chem 282(18): 13141-13145.
  24. Chernukhin, I., Shamsuddin, S., Kang, S. Y., Bergstrom, R., Kwon, Y. W., Yu, W., Whitehead, J., Mukhopadhyay, R., Docquier, F., Farrar, D., Morrison, I., Vigneron, M., Wu, S. Y., Chiang, C. M., Loukinov, D., Lobanenkov, V., Ohlsson, R. and Klenova, E. (2007). CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wide. Mol Cell Biol 27(5): 1631-1648.
  25. Lopez, A. B., Wang, C., Huang, C. C., Yaman, I., Li, Y., Chakravarty, K., Johnson, P. F., Chiang, C. M., Snider, M. D., Wek, R. C. and Hatzoglou, M. (2007). A feedback transcriptional mechanism controls the level of the arginine/lysine transporter cat-1 during amino acid starvation. Biochem J 402(1): 163-173.
  26. Wu, S. Y., Lee, A. Y., Hou, S. Y., Kemper, J. K., Erdjument-Bromage, H., Tempst, P. and Chiang, C. M. (2006). Brd4 links chromatin targeting to HPV transcriptional silencing. Genes Dev 20(17): 2383-2396.
  27. Kim, Y. K., Bourgeois, C. F., Pearson, R., Tyagi, M., West, M. J., Wong, J., Wu, S. Y., Chiang, C. M. and Karn, J. (2006). Recruitment of TFIIH to the HIV LTR is a rate-limiting step in the emergence of HIV from latency. EMBO J 25(15): 3596-3604.
  28. Thomas, M. C. and Chiang, C. M. (2006). The general transcription machinery and general cofactors. Crit Rev Biochem Mol Biol 41(3): 105-178.
  29. Dao, L. D., Duffy, A., Van Tine, B. A., Wu, S. Y., Chiang, C. M., Broker, T. R. and Chow, L. T. (2006). Dynamic localization of the human papillomavirus type 11 origin binding protein E2 through mitosis while in association with the spindle apparatus. J Virol 80(10): 4792-4800.
  30. Hou, S.Y. and Chiang, C. M. (2006). The general transcription machinery and preinitiation complex formation. In Gene Expression and Regulation. Ma, J. (eds). a Current Scientific Frontiers Book, Higher Education Press (Beijing) & Springer, pp. 21-48.
  31. Thomas, M.C. and Chiang, C. M. (2006). General Cofactors: TFIID, Mediator and USA. In Gene Expression and Regulation. Ma, J. (eds). a Current Scientific Frontiers Book, Higher Education Press (Beijing) & Springer, pp. 67-94.
  32. Thomas, M. C. and Chiang, C. M. (2005). E6 oncoprotein represses p53-dependent gene activation via inhibition of protein acetylation independently of inducing p53 degradation. Mol Cell 17(2): 251-264.
Protocols by Cheng-Ming Chiang
  1. Bimolecular Fluorescence Complementation (BiFC) Assay for Direct Visualization of Protein-Protein Interaction in vivo