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Keisuke Okita

Education

Doctor of Medicine, Faculty of Medical Sciences, Kumamoto University, 2004

Current position

Lecturer, Center for iPS cell Research and Application, Kyoto University, Kyoto, Japan

Publications

  1. Kondo, T., Asai, M., Tsukita, K., Kutoku, Y., Ohsawa, Y., Sunada, Y., Imamura, K., Egawa, N., Yahata, N., Okita, K., Takahashi, K., Asaka, I., Aoi, T., Watanabe, A., Watanabe, K., Kadoya, C., Nakano, R., Watanabe, D., Maruyama, K., Hori, O., Hibino, S., Choshi, T., Nakahata, T., Hioki, H., Kaneko, T., Naitoh, M., Yoshikawa, K., Yamawaki, S., Suzuki, S., Hata, R., Ueno, S., Seki, T., Kobayashi, K., Toda, T., Murakami, K., Irie, K., Klein, W. L., Mori, H., Asada, T., Takahashi, R., Iwata, N., Yamanaka, S. and Inoue, H. (2013). Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Abeta and differential drug responsiveness. Cell Stem Cell 12(4): 487-496.
  2. Okita, K., Yamakawa, T., Matsumura, Y., Sato, Y., Amano, N., Watanabe, A., Goshima, N. and Yamanaka, S. (2013). An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells 31(3): 458-466.
  3. Egawa, N., Kitaoka, S., Tsukita, K., Naitoh, M., Takahashi, K., Yamamoto, T., Adachi, F., Kondo, T., Okita, K., Asaka, I., Aoi, T., Watanabe, A., Yamada, Y., Morizane, A., Takahashi, J., Ayaki, T., Ito, H., Yoshikawa, K., Yamawaki, S., Suzuki, S., Watanabe, D., Hioki, H., Kaneko, T., Makioka, K., Okamoto, K., Takuma, H., Tamaoka, A., Hasegawa, K., Nonaka, T., Hasegawa, M., Kawata, A., Yoshida, M., Nakahata, T., Takahashi, R., Marchetto, M. C., Gage, F. H., Yamanaka, S. and Inoue, H. (2012). Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4(145): 145ra104.
  4. Kajiwara, M., Aoi, T., Okita, K., Takahashi, R., Inoue, H., Takayama, N., Endo, H., Eto, K., Toguchida, J., Uemoto, S. and Yamanaka, S. (2012). Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells. Proc Natl Acad Sci U S A 109(31): 12538-12543.
  5. Hirano, K., Nagata, S., Yamaguchi, S., Nakagawa, M., Okita, K., Kotera, H., Ainscough, J. and Tada, T. (2012). Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitors. Stem Cells Dev 21(8): 1287-1298.
  6. Iwabuchi, K. A., Yamakawa, T., Sato, Y., Ichisaka, T., Takahashi, K., Okita, K. and Yamanaka, S. (2011). ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency. PLoS One 6(5): e20461.
  7. Okita, K., Matsumura, Y., Sato, Y., Okada, A., Morizane, A., Okamoto, S., Hong, H., Nakagawa, M., Tanabe, K., Tezuka, K., Shibata, T., Kunisada, T., Takahashi, M., Takahashi, J., Saji, H. and Yamanaka, S. (2011). A more efficient method to generate integration-free human iPS cells. Nat Methods 8(5): 409-412.
  8. Fujiwara, M., Yan, P., Otsuji, T. G., Narazaki, G., Uosaki, H., Fukushima, H., Kuwahara, K., Harada, M., Matsuda, H., Matsuoka, S., Okita, K., Takahashi, K., Nakagawa, M., Ikeda, T., Sakata, R., Mummery, C. L., Nakatsuji, N., Yamanaka, S., Nakao, K. and Yamashita, J. K. (2011). Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A. PLoS One 6(2): e16734.
  9. Egusa, H., Okita, K., Kayashima, H., Yu, G., Fukuyasu, S., Saeki, M., Matsumoto, T., Yamanaka, S. and Yatani, H. (2010). Gingival fibroblasts as a promising source of induced pluripotent stem cells. PLoS One 5(9): e12743.
  10. Espejel, S., Roll, G. R., McLaughlin, K. J., Lee, A. Y., Zhang, J. Y., Laird, D. J., Okita, K., Yamanaka, S. and Willenbring, H. (2010). Induced pluripotent stem cell-derived hepatocytes have the functional and proliferative capabilities needed for liver regeneration in mice. J Clin Invest 120(9): 3120-3126.
  11. Saunders, L. R., Sharma, A. D., Tawney, J., Nakagawa, M., Okita, K., Yamanaka, S., Willenbring, H. and Verdin, E. (2010). miRNAs regulate SIRT1 expression during mouse embryonic stem cell differentiation and in adult mouse tissues. Aging (Albany NY) 2(7): 415-431.
  12. Okita, K., Hong, H., Takahashi, K. and Yamanaka, S. (2010). Generation of mouse-induced pluripotent stem cells with plasmid vectors. Nat Protoc 5(3): 418-428.
  13. Sato, S., Yagi, S., Arai, Y., Hirabayashi, K., Hattori, N., Iwatani, M., Okita, K., Ohgane, J., Tanaka, S., Wakayama, T., Yamanaka, S. and Shiota, K. (2010). Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells. Genes Cells 15(6): 607-618.
  14. Yoshida, Y., Takahashi, K., Okita, K., Ichisaka, T. and Yamanaka, S. (2009). Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell 5(3): 237-241.
  15. Hong, H., Takahashi, K., Ichisaka, T., Aoi, T., Kanagawa, O., Nakagawa, M., Okita, K. and Yamanaka, S. (2009). Suppression of induced pluripotent stem cell generation by the p53-p21 pathway. Nature 460(7259): 1132-1135.
  16. Senju, S., Haruta, M., Matsunaga, Y., Fukushima, S., Ikeda, T., Takahashi, K., Okita, K., Yamanaka, S. and Nishimura, Y. (2009). Characterization of dendritic cells and macrophages generated by directed differentiation from mouse induced pluripotent stem cells. Stem Cells 27(5): 1021-1031.
  17. Tsubooka, N., Ichisaka, T., Okita, K., Takahashi, K., Nakagawa, M. and Yamanaka, S. (2009). Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts. Genes Cells 14(6): 683-694.
  18. Niwa, A., Umeda, K., Chang, H., Saito, M., Okita, K., Takahashi, K., Nakagawa, M., Yamanaka, S., Nakahata, T. and Heike, T. (2009). Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitors. J Cell Physiol 221(2): 367-377.
  19. Hirami, Y., Osakada, F., Takahashi, K., Okita, K., Yamanaka, S., Ikeda, H., Yoshimura, N. and Takahashi, M. (2009). Generation of retinal cells from mouse and human induced pluripotent stem cells. Neurosci Lett 458(3): 126-131.
  20. Nishimura, K., Nakagawa, T., Ono, K., Ogita, H., Sakamoto, T., Yamamoto, N., Okita, K., Yamanaka, S. and Ito, J. (2009). Transplantation of mouse induced pluripotent stem cells into the cochlea. Neuroreport 20(14): 1250-1254.
  21. Miura, K., Okada, Y., Aoi, T., Okada, A., Takahashi, K., Okita, K., Nakagawa, M., Koyanagi, M., Tanabe, K., Ohnuki, M., Ogawa, D., Ikeda, E., Okano, H. and Yamanaka, S. (2009). Variation in the safety of induced pluripotent stem cell lines. Nat Biotechnol 27(8): 743-745.
  22. Okita, K., Nakagawa, M., Hyenjong, H., Ichisaka, T. and Yamanaka, S. (2008). Generation of mouse induced pluripotent stem cells without viral vectors. Science 322(5903): 949-953.
  23. Aoi, T., Yae, K., Nakagawa, M., Ichisaka, T., Okita, K., Takahashi, K., Chiba, T. and Yamanaka, S. (2008). Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science 321(5889): 699-702.
  24. Nakagawa, M., Koyanagi, M., Tanabe, K., Takahashi, K., Ichisaka, T., Aoi, T., Okita, K., Mochiduki, Y., Takizawa, N. and Yamanaka, S. (2008). Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 26(1): 101-106.
  25. Takahashi, K., Okita, K., Nakagawa, M. and Yamanaka, S. (2007). Induction of pluripotent stem cells from fibroblast cultures. Nat Protoc 2(12): 3081-3089.
  26. Okita, K., Ichisaka, T. and Yamanaka, S. (2007). Generation of germline-competent induced pluripotent stem cells. Nature 448(7151): 313-317.
Protocols by Keisuke Okita
  1. iPS Cell Induction from Human Non-T, B cells from Peripheral Blood