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Masahiro Morita

Education

Ph.D., Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo, 2008

Current position

Postdoctoral fellow, Department of Biochemistry and Goodman Cancer Research Centre, McGill University

Publications

  1. Morita, M., Gravel, S. P., Hulea, L., Larsson, O., Pollak, M., St-Pierre, J. and Topisirovic, I. (2015). mTOR coordinates protein synthesis, mitochondrial activity and proliferation. Cell Cycle 14(4): 473-480.
  2. El-Assaad, W., El-Kouhen, K., Mohammad, A. H., Yang, J., Morita, M., Gamache, I., Mamer, O., Avizonis, D., Hermance, N., Kersten, S., Tremblay, M. L., Kelliher, M. A. and Teodoro, J. G. (2015). Deletion of the gene encoding G0/G 1 switch protein 2 (G0s2) alleviates high-fat-diet-induced weight gain and insulin resistance, and promotes browning of white adipose tissue in mice. Diabetologia 58(1): 149-157.
  3. Watanabe, C., Morita, M., Hayata, T., Nakamoto, T., Kikuguchi, C., Li, X., Kobayashi, Y., Takahashi, N., Notomi, T., Moriyama, K., Yamamoto, T., Ezura, Y. and Noda, M. (2014). Stability of mRNA influences osteoporotic bone mass via CNOT3. Proc Natl Acad Sci U S A 111(7): 2692-2697.
  4. Shirai, Y. T., Suzuki, T., Morita, M., Takahashi, A. and Yamamoto, T. (2014). Multifunctional roles of the mammalian CCR4-NOT complex in physiological phenomena. Front Genet 5: 286. 
  5. Rouya, C., Siddiqui, N., Morita, M., Duchaine, T. F., Fabian, M. R. and Sonenberg, N. (2014). Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1. RNA 20(9): 1398-1409.
  6. Gandin, V., Sikstrom, K., Alain, T., Morita, M., McLaughlan, S., Larsson, O. and Topisirovic, I. (2014). Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale. J Vis Exp (87).
  7. Morita, M., Gravel, S. P., Chenard, V., Sikstrom, K., Zheng, L., Alain, T., Gandin, V., Avizonis, D., Arguello, M., Zakaria, C., McLaughlan, S., Nouet, Y., Pause, A., Pollak, M., Gottlieb, E., Larsson, O., St-Pierre, J., Topisirovic, I. and Sonenberg, N. (2013). mTORC1 controls mitochondrial activity and biogenesis through 4E-BP-dependent translational regulation. Cell Metab 18(5): 698-711.
  8. Alain, T.*, Morita, M.*, Fonseca, B. D., Yanagiya, A., Siddiqui, N., Bhat, M., Zammit, D., Marcus, V., Metrakos, P., Voyer, L. A., Gandin, V., Liu, Y., Topisirovic, I. and Sonenberg, N. (2012). eIF4E/4E-BP ratio predicts the efficacy of mTOR targeted therapies. Cancer Res 72(24): 6468-6476.
  9. Takahashi, A., Morita, M., Yokoyama, K., Suzuki, T. and Yamamoto, T. (2012). Tob2 inhibits peroxisome proliferator-activated receptor gamma2 expression by sequestering Smads and C/EBPalpha during adipocyte differentiation. Mol Cell Biol 32(24): 5067-5077.
  10. Morita, M., Ler, L. W., Fabian, M. R., Siddiqui, N., Mullin, M., Henderson, V. C., Alain, T., Fonseca, B. D., Karashchuk, G., Bennett, C. F., Kabuta, T., Higashi, S., Larsson, O., Topisirovic, I., Smith, R. J., Gingras, A. C. and Sonenberg, N. (2012). A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development. Mol Cell Biol 32(17): 3585-3593.
  11. Larsson, O.*, Morita, M.*, Topisirovic, I.*, Alain, T., Blouin, M. J., Pollak, M. and Sonenberg, N. (2012). Distinct perturbation of the translatome by the antidiabetic drug metformin. Proc Natl Acad Sci U S A 109(23): 8977-8982.
  12. Takahashi, A., Kikuguchi, C., Morita, M., Shimodaira, T., Tokai-Nishizumi, N., Yokoyama, K., Ohsugi, M., Suzuki, T. and Yamamoto, T. (2012). Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression. Biochem Biophys Res Commun 419(2): 268-273.
  13. Fabian, M. R., Cieplak, M. K., Frank, F., Morita, M., Green, J., Srikumar, T., Nagar, B., Yamamoto, T., Raught, B., Duchaine, T. F. and Sonenberg, N. (2011). miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT. Nat Struct Mol Biol 18(11): 1211-1217.
  14. Ito, K., Takahashi, A., Morita, M., Suzuki, T. and Yamamoto, T. (2011). The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability. Protein Cell 2(9): 755-763.
  15. Morita, M., Oike, Y., Nagashima, T., Kadomatsu, T., Tabata, M., Suzuki, T., Nakamura, T., Yoshida, N., Okada, M. and Yamamoto, T. (2011). Obesity resistance and increased hepatic expression of catabolism-related mRNAs in Cnot3+/- mice. EMBO J 30(22): 4678-4691.
  16. Ito, K., Inoue, T., Yokoyama, K., Morita, M., Suzuki, T. and Yamamoto, T. (2011). CNOT2 depletion disrupts and inhibits the CCR4-NOT deadenylase complex and induces apoptotic cell death. Genes Cells 16(4): 368-379.
  17. Wang, H., Morita, M., Yang, X., Suzuki, T., Yang, W., Wang, J., Ito, K., Wang, Q., Zhao, C., Bartlam, M., Yamamoto, T. and Rao, Z. (2010). Crystal structure of the human CNOT6L nuclease domain reveals strict poly(A) substrate specificity. EMBO J 29(15): 2566-2576.
  18. Morita, M., Ito, K., Suzuki, T., Yamamoto, T. (2009). Involvement of the CCR4-NOT Deadenylase Complex in the Control of Cell Growth. Systems Biology (Proceeding paper), 3, 229-237.
  19. Yang, X., Morita, M., Wang, H., Suzuki, T., Yang, W., Luo, Y., Zhao, C., Yu, Y., Bartlam, M., Yamamoto, T. and Rao, Z. (2008). Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity. Nucleic Acids Res 36(21): 6872-6881.
  20. Miyasaka, T.*, Morita, M.*, Ito, K., Suzuki, T., Fukuda, H., Takeda, S., Inoue, J., Semba, K. and Yamamoto, T. (2008). Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex. Cancer Sci 99(4): 755-761.
  21. Morita, M., Suzuki, T., Nakamura, T., Yokoyama, K., Miyasaka, T. and Yamamoto, T. (2007). Depletion of mammalian CCR4b deadenylase triggers elevation of the p27Kip1 mRNA level and impairs cell growth. Mol Cell Biol 27(13): 4980-4990.
Protocols by Masahiro Morita
  1. Polysome Profiling Analysis