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Brian Pfleger

Education

Ph.D., Department of Chemical Engineering, University of California, Berkeley, 2005

Current position

Associate Professor of Chemical and Biological Engineering, Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI

Publications

Papers in refereed journals based on research performed as a graduate student
Author name in bold indicates Dr. Pfleger's research advisor. Author name with "*" indicates a student or postdoc from the same lab as Dr. Pfleger. In the candidate's field, it is the convention to list the leading senior investigator last.

  1. Pfleger, B. F., Fawzi, N. J.* and Keasling, J. D. (2005). Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Biotechnol Bioeng 92(5): 553-558.
  2. Pfleger, B. F., Pitera, D. J.*, Smolke, C. D.* and Keasling, J. D. (2006). Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol 24(8): 1027-1032.
  3. Pfleger, B. F., Pitera, D. J.*, Newman, J. D.*, Martin, V. J.* and Keasling, J. D. (2007). Microbial sensors for small molecules: development of a mevalonate biosensor. Metab Eng 9(1): 30-38.
  4. Lee, S. K.*, Chou, H. H.*, Pfleger, B. F., Newman, J. D.*, Yoshikuni, Y.* and Keasling, J. D. (2007). Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters. Appl Environ Microbiol 73(18): 5711-5715.
  5. Kizer, L.*, Pitera, D. J.*, Pfleger, B. F. and Keasling, J. D. (2008). Application of functional genomics to pathway optimization for increased isoprenoid production. Appl Environ Microbiol 74(10): 3229-3241.

Papers in refereed journals based on research performed as a postdoctoral fellow
Author name in bold indicates Dr. Pfleger's research advisor. Author name with "*" indicates a student or postdoc from the same lab as Dr. Pfleger. Author name in italics indicates collaborators from another lab. In the candidate's field, it is the convention to list the leading senior investigator last.

  1. Lee, J. Y.*, Janes, B. K., Passalacqua, K. D., Pfleger, B. F., Bergman, N. H., Liu, H., Hakansson, K., Somu, R. V., Aldrich, C. C., Cendrowski, S., Hanna, P. C. and Sherman, D. H. (2007). Biosynthetic analysis of the petrobactin siderophore pathway from Bacillus anthracis. J Bacteriol 189(5): 1698-1710.
  2. Pfleger, B. F., Lee, J. Y.*, Somu, R. V., Aldrich, C. C., Hanna, P. C. and Sherman, D. H. (2007). Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis. Biochemistry 46(13): 4147-4157.
  3. Pfleger, B. F., Kim, Y., Nusca, T. D.*, Maltseva, N., Lee, J. Y.*, Rath, C. M.*, Scaglione, J. B.*, Janes, B. K., Anderson, E. C., Bergman, N. H., Hanna, P. C., Joachimiak, A. and Sherman, D. H. (2008). Structural and functional analysis of AsbF: origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis. Proc Natl Acad Sci U S A 105(44): 17133-17138.
    In Editors' Choice: Highlights of the recent literature, Science 28 Nov (2008) 322:1304
  4. Nusca, T. D.*, Kim, Y., Maltseva, N., Lee, J. Y., Eschenfeldt, W., Stols, L., Schofield, M. M., Scaglione, J. B.*, Dixon, S. D., Oves-Costales, D., Challis, G. L., Hanna, P. C., Pfleger, B. F., Joachimiak, A. and Sherman, D. H. (2012). Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from Bacillus anthracis. J Biol Chem 287(19): 16058-16072.

Papers in refereed journals based on research performed at the University of Wisconsin–Madison
Author names in bold indicate faculty. Author names with "*" indicate undergraduate researchers. Author names with "#" indicate high school students. Author names in italics indicate collaborators from another lab.

  1. Lennen, R. M., Braden, D. J., West, R. A., Dumesic, J. A. and Pfleger, B. F. (2010). A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnol Bioeng 106(2): 193-202.
    • Highlighted by editors in the issue's table of contents.
  2. Hoover, S. W., Marner, W. D., 2nd, Brownson, A. K.*, Lennen, R. M., Wittkopp, T. M., Yoshitani, J., Zulkifly, S., Graham, L. E., Chaston, S. D., McMahon, K. D. and Pfleger, B. F. (2011). Bacterial production of free fatty acids from freshwater macroalgal cellulose. Appl Microbiol Biotechnol 91(2): 435-446.
  3. Mendez-Perez, D., Begemann, M. B. and Pfleger, B. F. (2011). Modular synthase-encoding gene involved in α-olefin biosynthesis in Synechococcus sp. strain PCC 7002. Appl Environ Microbiol 77(12): 4264-4267.
  4. Lennen, R. M., Kruziki, M. A.*, Kumar, K.*, Zinkel, R. A., Burnum, K. E., Lipton, M. S., Hoover, S. W., Ranatunga, D. R., Wittkopp, T. M., Marner, W. D., 2nd and Pfleger, B. F. (2011). Membrane stresses induced by overproduction of free fatty acids in Escherichia coli. Appl Environ Microbiol 77(22): 8114-8128.
  5. Agnew, D. E. and Pfleger, B. F. (2011). Optimization of synthetic operons using libraries of post-transcriptional regulatory elements. Methods Mol Biol 765: 99-111.
  6. Hoover, S. W., Tyler Youngquist, J., Angart, P. A.*, Withers, S. T., Lennen, R. M. and Pfleger, B. F. (2012). Isolation of improved free fatty acid overproducing strains of Escherichia coli via nile red based high‐throughput screening. Environmental Progress & Sustainable Energy 31(1): 17-23.
  7. Graham, J. M., Graham, L. E., Zulkifly, S. B., Pfleger, B. F., Hoover, S. W. and Yoshitani, J. (2012). Freshwater diatoms as a source of lipids for biofuels. J Ind Microbiol Biotechnol 39(3): 419-428.
  8. Youngquist, J. T., Lennen, R. M., Ranatunga, D. R., Bothfeld, W. H., Marner, W. D., 2nd and Pfleger, B. F. (2012). Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain. Biotechnol Bioeng 109(6): 1518-1527.
  9. Mendez-Perez, D., Gunasekaran, S.#, Orler, V. J.* and Pfleger, B. F. (2012). A translation-coupling DNA cassette for monitoring protein translation in Escherichia coli. Metab Eng 14(4): 298-305.
  10. Agnew, D. E., Stevermer, A. K.*, Youngquist, J. T. and Pfleger, B. F. (2012). Engineering Escherichia coli for production of C(1)(2)-C(1)(4) polyhydroxyalkanoate from glucose. Metab Eng 14(6): 705-713.
  11. Lennen, R. M. and Pfleger, B. F. (2012). Engineering Escherichia coli to synthesize free fatty acids. Trends Biotechnol 30(12): 659-667.
  12. Lennen, R. M., Politz, M. G., Kruziki, M. A.* and Pfleger, B. F. (2013). Identification of transport proteins involved in free fatty acid efflux in Escherichia coli. J Bacteriol 195(1): 135-144.
  13. Politz, M. C., Copeland, M. F. and Pfleger, B. F. (2013). Artificial repressors for controlling gene expression in bacteria. Chem Commun (Camb) 49(39): 4325-4327.
    • Invited articles for the Young Investigators Issue.
  14. Lennen, R. M. and Pfleger, B. F. (2012). Engineering Escherichia coli to synthesize free fatty acids. Trends Biotechnol 30(12): 659-667.
  15. Agnew, D. E., Pfleger, B. F. Synthetic biology strategies for synthesizing polyhydroxyalkanoates from unrelated carbon sources Chemical Engineering Science (Epub ahead of press).
  16. Lennen, R. M. and Pfleger, B. F. (2013). Microbial production of fatty acid-derived fuels and chemicals. Curr Opin Biotechnol.
  17. Youngquist, J. T., Rose, J. P.* and Pfleger, B. F. (2013). Free fatty acid production in Escherichia coli under phosphate-limited conditions. Appl Microbiol Biotechnol 97(11): 5149-5159.
  18. Pfleger, B. F. (2013). By-passing the refinery for production of high-value BTX derivatives. Biotechnol J. [Epub ahead of print]
  19. Begemann, M. B., Zess, E. K., Walters, E. M., Schmitt, E. F.*, Markley, A. L. and Pfleger, B. F. (2013). An organic Acid based counter selection system for cyanobacteria. PLoS One 8(10): e76594.

Book sections edited

  1. Pfleger, B. F. (2009). Future Applications of Metabolic Engineering. In The Metabolic Engineering Handbook. Smolke, C.D. (eds). San Diego: CRC Press.

Papers submitted to refereed journals but not yet accepted

  1. Youngquist, J. T., Schumacher, M. H.*, Rose, J. P.*, Raines, T. C.*, Politz, M. C., Copeland, M. F. and Pfleger, B. F. (2013). Production of medium chain length fatty alcohols from glucose in Escherichia coli. Metab Eng.
  2. Luterbacher. J. S., Rand, J. M., Alonso, D. M., Youngquist, J.T., Pfleger, B. F., Dumesic, J. A. Non-enzymatic sugar production from biomass using biomass-derived γ-valerolactone. (in Revision)
  3. Mendez-Perez, D., Herman, N. A.*, Pfleger, B. F. A desaturase gene involved in formation of 1,14 nonadecadiene produced by Synechococcus sp. Strain PCC 7002. (in Preparation)

Patents

Patents/Patent applications from research performed as a graduate student
Inventor name in bold indicates Dr. Pfleger's research advisor. Inventor names with "*" indicate a student or postdoc from the same lab as Dr. Pfleger. Inventor name in italics indicates collaborators from another lab.

  1. Pitera, D. J.*, Newman, J. D.*, Kizer, J. L.*, Keasling, J. D. Pfleger, B. F. (2012). US 8,114,645 B2 – METHODS FOR INCREASING ISOPRENOID AND ISOPRENOID PRECURSOR PRODUCTION BY MODULATING FATTY ACID LEVELS. Issued February 14th.
  2. Keasling, J. D., Pitera, D. J.*, Paddon, C. J., Pfleger, B. F. Patent Application - US2007/015498 - WO/2008/008256 – METHODS FOR ENHANCING PRODUCTION OF ISOPRENOID COMPOUNDS BY HOST CELLS.

Patents/Patent applications from research performed at UW-Madison

  1. Pfleger, B. F., Lennen, R. M. U. S. Patent Application 12/984,343–US 2011/0165637–FATTY ACID- PRODUCING HOSTS.
  2. Pfleger, B. F., Mendez-Perez, D. U. S. Provisional Patent Application 61/289,739–US 2012/0021922 A1 – TRANSLATION-COUPLING SYSTEMS.
  3. Pfleger, B. F., Agnew, D. A. U. S. Provisional patent application 13/833230. PRODUCTION OF POLYHYDROXYALKANOATES WITH A DEFINED COMPOSITION FROM AN UNRELATED CARBON SOURCE.
  4. Pfleger, B. F., Begemann, M. B. U. S. Provisional patent application 13/798835. ORGANIC ACID- TOLERANT MICROORGANISMS AND USES THEREOF FOR PRODUCING ORGANIC ACIDS.
  5. Dumesic. J. A., Pfleger, B. F., Luterbacher, J. S., Rand, J. U. S. Provisional patent application 13/918097. BIOLOGICAL CONVERSION OF BIOMASS-DERIVED SUGARS TO VALUE-ADDED CHEMICALS.
Protocols by Brian Pfleger
  1. Quantification of Bacterial Fatty Acids by Extraction and Methylation