Welcome guest, Login | Register

Home


Bruno Verhasselt

Education

MD Ghent University, Belgium, 1992
PhD Ghent University, Belgium, 1999

Current position

Associate professor , Ghent University
Staff Laboratory Medicine, Ghent University Hospital
Clincal Investigator, Research Foundation - Flanders (FWO)

Publications (since 2005):

  1. Tency, I., Verstraelen, H., Saerens, B., Verhasselt, B., Vaneechoutte, M., Degomme, O., Verhelst, R. and Temmerman, M. (2013). Elevated soluble triggering receptor expressed on myeloid cells (sTREM)-1 levels in maternal serum during term and preterm labor. PLoS One 8(2): e56050.
  2. Pede, V., Rombout, A., Vermeire, J., Naessens, E., Mestdagh, P., Robberecht, N., Vanderstraeten, H., Van Roy, N., Vandesompele, J., Speleman, F., Philippe, J. and Verhasselt, B. (2013). CLL cells respond to B-Cell receptor stimulation with a microRNA/mRNA signature associated with MYC activation and cell cycle progression. PLoS One 8(4): e60275.
  3. Meuwissen, P. J., Stolp, B., Iannucci, V., Vermeire, J., Naessens, E., Saksela, K., Geyer, M., Vanham, G., Arien, K. K., Fackler, O. T. and Verhasselt, B. (2012). Identification of a highly conserved valine-glycine-phenylalanine amino acid triplet required for HIV-1 Nef function. Retrovirology 9: 34.
  4. Impens, F., Timmerman, E., Staes, A., Moens, K., Arien, K. K., Verhasselt, B., Vandekerckhove, J. and Gevaert, K. (2012). A catalogue of putative HIV-1 protease host cell substrates. Biol Chem 393(9): 915-931.
  5. Tency, I., Verstraelen, H., Kroes, I., Holtappels, G., Verhasselt, B., Vaneechoutte, M., Verhelst, R. and Temmerman, M. (2012). Imbalances between matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in maternal serum during preterm labor. PLoS One 7(11): e49042.
  6. Messiaen, P., De Spiegelaere, W., Alcami, J., Vervisch, K., Van Acker, P., Verhasselt, B., Meuwissen, P., Calonge, E., Gonzalez, N., Gutierrez-Rodero, F., Rodriguez-Martin, C., Sermijn, E., Poppe, B., Vogelaers, D., Verhofstede, C. and Vandekerckhove, L. (2012). Characterization of LEDGF/p75 genetic variants and association with HIV-1 disease progression. PLoS One 7(11): e50204.
  7. Vermeire, J., Naessens, E., Vanderstraeten, H., Landi, A., Iannucci, V., Van Nuffel, A., Taghon, T., Pizzato, M. and Verhasselt, B. (2012). Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors. PLoS One 7(12): e50859.
  8. Vermeire, J., Vanbillemont, G., Witkowski, W. and Verhasselt, B. (2011). The Nef-infectivity enigma: mechanisms of enhanced lentiviral infection. Curr HIV Res 9(7): 474-489.
  9. Landi, A., Iannucci, V., Nuffel, A. V., Meuwissen, P. and Verhasselt, B. (2011). One protein to rule them all: modulation of cell surface receptors and molecules by HIV Nef. Curr HIV Res 9(7): 496-504.
  10. Delputte, P. L., Van Gorp, H., Favoreel, H. W., Hoebeke, I., Delrue, I., Dewerchin, H., Verdonck, F., Verhasselt, B., Cox, E. and Nauwynck, H. J. (2011). Porcine sialoadhesin (CD169/Siglec-1) is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages. PLoS One 6(2): e16827.
  11. De Weer, A., Van der Meulen, J., Rondou, P., Taghon, T., Konrad, T. A., De Preter, K., Mestdagh, P., Van Maerken, T., Van Roy, N., Jeison, M., Yaniv, I., Cauwelier, B., Noens, L., Poirel, H. A., Vandenberghe, P., Lambert, F., De Paepe, A., Sanchez, M. G., Odero, M., Verhasselt, B., Philippe, J., Vandesompele, J., Wieser, R., Dastugue, N., Van Vlierberghe, P., Poppe, B. and Speleman, F. (2011). EVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells. Br J Haematol 154(3): 337-348.
  12. Verhasselt, B. (2011). Editorial. Nef: a notorious enigmatic factor in HIV biology. Curr HIV Res 9(7): 473.
  13. Smits, K., Iannucci, V., Stove, V., Van Hauwe, P., Naessens, E., Meuwissen, P. J., Arien, K. K., Bentahir, M., Plum, J. and Verhasselt, B. (2010). Rho GTPase Cdc42 is essential for human T-cell development. Haematologica 95(3): 367-375.
  14. Denys, B., El Housni, H., Nollet, F., Verhasselt, B. and Philippe, J. (2010). A real-time polymerase chain reaction assay for rapid, sensitive, and specific quantification of the JAK2V617F mutation using a locked nucleic acid-modified oligonucleotide. J Mol Diagn 12(4): 512-519.
  15. Van Bockstaele, F., Verhasselt, B. and Philippe, J. (2009). Prognostic markers in chronic lymphocytic leukemia: a comprehensive review. Blood Rev 23(1): 25-47.
  16. Van Vlierberghe, P., De Weer, A., Mestdagh, P., Feys, T., De Preter, K., De Paepe, P., Lambein, K., Vandesompele, J., Van Roy, N., Verhasselt, B., Poppe, B. and Speleman, F. (2009). Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs. Br J Haematol 147(5): 686-690.
  17. De Weer, A., Speleman, F., Cauwelier, B., Van Roy, N., Yigit, N., Verhasselt, B., De Moerloose, B., Benoit, Y., Noens, L., Selleslag, D., Lippert, E., Struski, S., Bastard, C., De Paepe, A., Vandenberghe, P., Hagemeijer, A., Dastugue, N. and Poppe, B. (2008). EVI1 overexpression in t(3;17) positive myeloid malignancies results from juxtaposition of EVI1 to the MSI2 locus at 17q22. Haematologica 93(12): 1903-1907.
  18. Van Bockstaele, F., Pede, V., Naessens, E., Van Coppernolle, S., Van Tendeloo, V., Verhasselt, B. and Philippe, J. (2008). Efficient gene transfer in CLL by mRNA electroporation. Leukemia 22(2): 323-329.
  19. Arien, K. K. and Verhasselt, B. (2008). HIV Nef: role in pathogenesis and viral fitness. Curr HIV Res 6(3): 200-208.
  20. De Weer, A., Poppe, B., Cauwelier, B., Carlier, A., Dierick, J., Verhasselt, B., Philippe, J., Van Roy, N. and Speleman, F. (2008). EVI1 activation in blast crisis CML due to juxtaposition to the rare 17q22 partner region as part of a 4-way variant translocation t(9;22). BMC Cancer 8: 193.
  21. Van Hamme, E., Dewerchin, H. L., Cornelissen, E., Verhasselt, B. and Nauwynck, H. J. (2008). Clathrin- and caveolae-independent entry of feline infectious peritonitis virus in monocytes depends on dynamin. J Gen Virol 89(Pt 9): 2147-2156.
  22. Dewerchin, H. L., Cornelissen, E., Van Hamme, E., Smits, K., Verhasselt, B. and Nauwynck, H. J. (2008). Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway. J Gen Virol 89(Pt 11): 2731-2740.
  23. Cauwelier, B., Cave, H., Gervais, C., Lessard, M., Barin, C., Perot, C., Van den Akker, J., Mugneret, F., Charrin, C., Pages, M. P., Gregoire, M. J., Jonveaux, P., Lafage-Pochitaloff, M., Mozzicconacci, M. J., Terre, C., Luquet, I., Cornillet-Lefebvre, P., Laurence, B., Plessis, G., Lefebvre, C., Leroux, D., Antoine-Poirel, H., Graux, C., Mauvieux, L., Heimann, P., Chalas, C., Clappier, E., Verhasselt, B., Benoit, Y., Moerloose, B. D., Poppe, B., Van Roy, N., Keersmaecker, K. D., Cools, J., Sigaux, F., Soulier, J., Hagemeijer, A., Paepe, A. D., Dastugue, N., Berger, R. and Speleman, F. (2007). Clinical, cytogenetic and molecular characteristics of 14 T-ALL patients carrying the TCRbeta-HOXA rearrangement: a study of the Groupe Francophone de Cytogenetique Hematologique. Leukemia 21(1): 121-128.
  24. Van Bockstaele, F., Pede, V., Janssens, A., Callewaert, F., Offner, F., Verhasselt, B. and Philippe, J. (2007). Lipoprotein lipase mRNA expression in whole blood is a prognostic marker in B cell chronic lymphocytic leukemia. Clin Chem 53(2): 204-212.
  25. Sourisseau, M., Schilte, C., Casartelli, N., Trouillet, C., Guivel-Benhassine, F., Rudnicka, D., Sol-Foulon, N., Le Roux, K., Prevost, M. C., Fsihi, H., Frenkiel, M. P., Blanchet, F., Afonso, P. V., Ceccaldi, P. E., Ozden, S., Gessain, A., Schuffenecker, I., Verhasselt, B., Zamborlini, A., Saib, A., Rey, F. A., Arenzana-Seisdedos, F., Despres, P., Michault, A., Albert, M. L. and Schwartz, O. (2007). Characterization of reemerging chikungunya virus. PLoS Pathog 3(6): e89.
  26. Feys, T., Poppe, B., De Preter, K., Van Roy, N., Verhasselt, B., De Paepe, P., De Paepe, A. and Speleman, F. (2007). A detailed inventory of DNA copy number alterations in four commonly used Hodgkin's lymphoma cell lines. Haematologica 92(7): 913-920.
  27. Smits, K., De Smedt, M., Naessens, E., De Smet, G., Stove, V., Taghon, T., Plum, J. and Verhasselt, B. (2007). Tumor necrosis factor promotes T-cell at the expense of B-cell lymphoid development from cultured human CD34+ cord blood cells. Exp Hematol 35(8): 1272-1278.
  28. Taes, Y. E., Goemaere, S., Huang, G., Van Pottelbergh, I., De Bacquer, D., Verhasselt, B., Van den Broeke, C., Delanghe, J. R. and Kaufman, J. M. (2006). Vitamin D binding protein, bone status and body composition in community-dwelling elderly men. Bone 38(5): 701-707.
  29. Janssens, A., Berth, M., De Paepe, P., Verhasselt, B., Van Roy, N., Noens, L., Philippe, J. and Offner, F. (2006). EBV negative Richter's syndrome from a coexistent clone after salvage treatment with alemtuzumab in a CLL patient. Am J Hematol 81(9): 706-712.
  30. Stove, V. and Verhasselt, B. (2006). Modelling thymic HIV-1 Nef effects. Curr HIV Res 4(1): 57-64.
  31. Tielens, S., Verhasselt, B., Liu, J., Dhont, M., Van Der Elst, J. and Cornelissen, M. (2006). Generation of embryonic stem cell lines from mouse blastocysts developed in vivo and in vitro: relation to Oct-4 expression. Reproduction 132(1): 59-66.
  32. Delanghe, J., Verstraelen, H., Pynaert, I., Debels, L., Taes, Y., Verhasselt, B., De Henauw, S. and Temmerman, M. (2006). Human transferrin G277S mutation and iron deficiency in pregnancy. Br J Haematol 132(2): 249-250.
  33. Van Bockstaele, F., Janssens, A., Piette, A., Callewaert, F., Pede, V., Offner, F., Verhasselt, B. and Philippe, J. (2006). Kolmogorov-Smirnov statistical test for analysis of ZAP-70 expression in B-CLL, compared with quantitative PCR and IgV(H) mutation status. Cytometry B Clin Cytom 70(4): 302-308.
  34. 35- Stove V, Smits K, Naessens E, Plum J, Verhasselt B. Multiple gene knock-down by a single lentiviral vector expressing an array of short hairpin RNAs. Elect. J. Biotech., 9 (5)
    http://www.ejbiotechnology.info/content/vol9/issue5/full/13/index.html (2006) (ISI impactfactor 2006 = 0,725)
  35. Speleman, F., Cauwelier, B., Dastugue, N., Cools, J., Verhasselt, B., Poppe, B., Van Roy, N., Vandesompele, J., Graux, C., Uyttebroeck, A., Boogaerts, M., De Moerloose, B., Benoit, Y., Selleslag, D., Billiet, J., Robert, A., Huguet, F., Vandenberghe, P., De Paepe, A., Marynen, P. and Hagemeijer, A. (2005). A new recurrent inversion, inv(7)(p15q34), leads to transcriptional activation of HOXA10 and HOXA11 in a subset of T-cell acute lymphoblastic leukemias. Leukemia 19(3): 358-366.
  36. Stove, V., Van de Walle, I., Naessens, E., Coene, E., Stove, C., Plum, J. and Verhasselt, B. (2005). Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8alphabeta. J Virol 79(17): 11422-11433.
  37. Poppe, B., Cauwelier, B., Van Limbergen, H., Yigit, N., Philippe, J., Verhasselt, B., De Paepe, A., Benoit, Y. and Speleman, F. (2005). Novel cryptic chromosomal rearrangements in childhood acute lymphoblastic leukemia detected by multiple color fluorescent in situ hybridization. Haematologica 90(9): 1179-1185.
  38. Claeys, S., Van Hoecke, H., Holtappels, G., Gevaert, P., De Belder, T., Verhasselt, B., Van Cauwenberge, P. and Bachert, C. (2005). Nasal polyps in patients with and without cystic fibrosis: a differentiation by innate markers and inflammatory mediators. Clin Exp Allergy 35(4): 467-472.
Protocols by Bruno Verhasselt
  1. Quantification of Retro- and Lentiviral Reverse Transcriptase Activity by Real-time PCR