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Hiroyuki   Konishi

Hiroyuki Konishi


M.D in Osaka University School of Medicine
Ph.D. in Medical Science, Osaka University Graduate School of Medicine

Current Position

Professor, Department of Biochemistry, Aichi Medical University School of Medicine, Japan


  1. Karnan, S., Ota, A., Konishi, Y., Wahiduzzaman, M., Hosokawa, Y. and Konishi, H. (2016). Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide. Nucleic Acids Res 44(6): e54.
  2. Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y. and Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated viral vectors. PLoS One 9(8): e106472.
  3. Karnan, S., Konishi, Y., Ota, A., Takahashi, M., Damdindorj, L., Hosokawa, Y. and Konishi, H. (2012). Simple monitoring of gene targeting efficiency in human somatic cell lines using the PIGA gene. PLoS One 7(10): e47389.
  4. Konishi, Y., Karnan, S., Takahashi, M., Ota, A., Damdindorj, L., Hosokawa, Y. and Konishi, H. (2012). A system for the measurement of gene targeting efficiency in human cell lines using an antibiotic resistance-GFP fusion gene. Biotechniques 53(3): 141-152.
  5. Damdindorj, L., Karnan, S., Ota, A., Takahashi, M., Konishi, Y., Hossain, E., Hosokawa, Y. and Konishi, H. (2012). Assessment of the long-term transcriptional activity of a 550-bp-long human beta-actin promoter region. Plasmid 68(3): 195-200.
  6. Konishi, H., Mohseni, M., Tamaki, A., Garay, J. P., Croessmann, S., Karnan, S., Ota, A., Wong, H. Y., Konishi, Y., Karakas, B., Tahir, K., Abukhdeir, A. M., Gustin, J. P., Cidado, J., Wang, G. M., Cosgrove, D., Cochran, R., Jelovac, D., Higgins, M. J., Arena, S., Hawkins, L., Lauring, J., Gross, A. L., Heaphy, C. M., Hosokawa, Y., Gabrielson, E., Meeker, A. K., Visvanathan, K., Argani, P., Bachman, K. E. and Park, B. H. (2011). Mutation of a single allele of the cancer susceptibility gene BRCA1 leads to genomic instability in human breast epithelial cells. Proc Natl Acad Sci U S A 108(43): 17773-17778.
  7. Karnan, S., Mohseni, M., Konishi, Y., Tamaki, A., Hosokawa, Y., Park, B. H. and Konishi, H. (2010). Controversial BRCA1 allelotypes in commonly used breast cancer cell lines. Breast Cancer Res Treat 119(1): 249-251.
  8. Konishi, H., Lauring, J., Garay, J. P., Karakas, B., Abukhdeir, A. M., Gustin, J. P., Konishi, Y. and Park, B. H. (2007). A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting. Nat Protoc 2(11): 2865-2874.
  9. Konishi, H., Karakas, B., Abukhdeir, A. M., Lauring, J., Gustin, J. P., Garay, J. P., Konishi, Y., Gallmeier, E., Bachman, K. E. and Park, B. H. (2007). Knock-in of mutant K-ras in nontumorigenic human epithelial cells as a new model for studying K-ras mediated transformation. Cancer Res 67(18): 8460-8467.
  10. Takamizawa, J., Konishi, H., Yanagisawa, K., Tomida, S., Osada, H., Endoh, H., Harano, T., Yatabe, Y., Nagino, M., Nimura, Y., Mitsudomi, T. and Takahashi, T. (2004). Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res 64(11): 3753-3756.
  11. Konishi, H., Sugiyama, M., Mizuno, K., Saito, H., Yatabe, Y., Takahashi, T., Osada, H. and Takahashi, T. (2003). Detailed characterization of a homozygously deleted region corresponding to a candidate tumor suppressor locus at distal 17p13.3 in human lung cancer. Oncogene 22: 1892-1905.
  12. Konishi, H., Nakagawa, T., Harano, T., Mizuno, K., Saito, H., Masuda, A., Matsuda, H., Osada, H. and Takahashi, T. (2002). Identification of frequent G2 checkpoint impairment and a homozygous deletion of 14-3-3ε at 17p13.3 in small cell lung cancers. Cancer Res, 62: 271-276, 2002.
  13. Konishi, H., Takahashi, T., Kozaki, K., Yatabe, Y., Mitsudomi, T., Fujii, Y., Sugiura, T., Matsuda, H., Takahashi, T. and Takahashi, T. (1998). Detailed deletion mapping suggests the involvement of a tumor suppressor gene at 17p13.3, distal to p53, in the pathogenesis of lung cancers. Oncogene 17(16): 2095-2100.
Protocols by Hiroyuki Konishi
  1. Efficient AAV-mediated Gene Targeting Using 2A-based Promoter-trap System