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Andrew W. Sylwester


Ph.D. in Cell Biology, Department of Biology, University of Iowa, 1996

Current position

Manager, Louis Picker Lab, Vaccine and Gene Therapy Institute, Oregon Health and Science University
Head, Infectious Disease Resource, Laboratory Unit, Oregon National Primate Research Center
Head, ONPRC Flow Cytometry Core

Publications (since 2004)

  1. Li, H., Margolick, J. B., Bream, J. H., Nilles, T. L., Langan, S., Bui, H. T., Sylwester, A. W., Picker, L. J. and Leng, S. X. (2014). Heterogeneity of CD4+ and CD8+ T-cell Responses to Cytomegalovirus in HIV-Infected and HIV-Uninfected Men Who Have Sex With Men. J Infect Dis. (Epub ahead of print)
  2. Park, H., Adamson, L., Ha, T., Mullen, K., Hagen, S. I., Nogueron, A., Sylwester, A. W., Axthelm, M. K., Legasse, A., Piatak, M., Jr., Lifson, J. D., McElrath, J. M., Picker, L. J. and Seder, R. A. (2013). Polyinosinic-polycytidylic acid is the most effective TLR adjuvant for SIV Gag protein-induced T cell responses in nonhuman primates. J Immunol 190(8): 4103-4115.
  3. Fukazawa, Y., Park, H., Cameron, M. J., Lefebvre, F., Lum, R., Coombes, N., Mahyari, E., Hagen, S. I., Bae, J. Y., Reyes, M. D., 3rd, Swanson, T., Legasse, A. W., Sylwester, A., Hansen, S. G., Smith, A. T., Stafova, P., Shoemaker, R., Li, Y., Oswald, K., Axthelm, M. K., McDermott, A., Ferrari, G., Montefiori, D. C., Edlefsen, P. T., Piatak, M., Jr., Lifson, J. D., Sekaly, R. P. and Picker, L. J. (2012). Lymph node T cell responses predict the efficacy of live attenuated SIV vaccines. Nat Med 18(11): 1673-1681.
  4. Donaldson, M. M., Kao, S. F., Eslamizar, L., Gee, C., Koopman, G., Lifton, M., Schmitz, J. E., Sylwester, A. W., Wilson, A., Hawkins, N., Self, S. G., Roederer, M. and Foulds, K. E. (2012). Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies. J Immunol Methods 386(1-2): 10-21.
  5. Okoye, A. A., Rohankhedkar, M., Abana, C., Pattenn, A., Reyes, M., Pexton, C., Lum, R., Sylwester, A., Planer, S. L., Legasse, A., Park, B. S., Piatak, M., Jr., Lifson, J. D., Axthelm, M. K. and Picker, L. J. (2012). Naive T cells are dispensable for memory CD4+ T cell homeostasis in progressive simian immunodeficiency virus infection. J Exp Med 209(4): 641-651.
  6. Cicin-Sain, L., Sylwester, A. W., Hagen, S. I., Siess, D. C., Currier, N., Legasse, A. W., Fischer, M. B., Koudelka, C. W., Axthelm, M. K., Nikolich-Zugich, J. and Picker, L. J. (2011). Cytomegalovirus-specific T cell immunity is maintained in immunosenescent rhesus macaques. J Immunol 187(4): 1722-1732.
  7. Okoye, A., Park, H., Rohankhedkar, M., Coyne-Johnson, L., Lum, R., Walker, J. M., Planer, S. L., Legasse, A. W., Sylwester, A. W., Piatak, M., Jr., Lifson, J. D., Sodora, D. L., Villinger, F., Axthelm, M. K., Schmitz, J. E. and Picker, L. J. (2009). Profound CD4+/CCR5+ T cell expansion is induced by CD8+ lymphocyte depletion but does not account for accelerated SIV pathogenesis. J Exp Med 206(7): 1575-1588.
  8. Okoye, A., Meier-Schellersheim, M., Brenchley, J. M., Hagen, S. I., Walker, J. M., Rohankhedkar, M., Lum, R., Edgar, J. B., Planer, S. L., Legasse, A., Sylwester, A. W., Piatak, M., Jr., Lifson, J. D., Maino, V. C., Sodora, D. L., Douek, D. C., Axthelm, M. K., Grossman, Z. and Picker, L. J. (2007). Progressive CD4+ central memory T cell decline results in CD4+ effector memory insufficiency and overt disease in chronic SIV infection. J Exp Med 204(9): 2171-2185.
  9. Sylwester, A. W., Mitchell, B. L., Edgar, J. B., Taormina, C., Pelte, C., Ruchti, F., Sleath, P. R., Grabstein, K. H., Hosken, N. A., Kern, F., Nelson, J. A. and Picker, L. J. (2005). Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects. J Exp Med 202(5): 673-685.
  10. Picker, L. J., Hagen, S. I., Lum, R., Reed-Inderbitzin, E. F., Daly, L. M., Sylwester, A. W., Walker, J. M., Siess, D. C., Piatak, M., Jr., Wang, C., Allison, D. B., Maino, V. C., Lifson, J. D., Kodama, T. and Axthelm, M. K. (2004). Insufficient production and tissue delivery of CD4+ memory T cells in rapidly progressive simian immunodeficiency virus infection. J Exp Med 200(10): 1299-1314.
Protocols by Andrew W. Sylwester
  1. Quantification of T Cell Antigen-specific Memory Responses in Rhesus Macaques, Using Cytokine Flow Cytometry (CFC, also Known as ICS and ICCS): Analysis of Flow Data
  2. Quantification of T Cell Antigen-specific Memory Responses in Rhesus Macaques, Using Cytokine Flow Cytometry (CFC, also Known as ICS and ICCS): from Assay Set-up to Data Acquisition