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Melinda Hauser

Education

Ph.D in Developmental and Cell Biology, University of California, Irvine, 1987

Current Position

Research Assistant Professor, Department of Microbiology, University of Tennessee, Knoxville, TN, USA

Publications

  1. Uddin, M. S., Hauser, M., Naider, F. and Becker, J. M. (2016). The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation. Biochim Biophys Acta 1858(4): 715-724.
  2. Rymer, J. K., Hauser, M., Bourdon, A. K., Campagna, S. R., Naider, F. and Becker, J. M. (2015). Novobiocin and peptide analogs of alpha-factor are positive allosteric modulators of the yeast G protein-coupled receptor Ste2p. Biochim Biophys Acta 1848(4): 916-924.
  3. Kim, K. M., Lee, Y. H., Akal-Strader, A., Uddin, M. S., Hauser, M., Naider, F. and Becker, J. M. (2012). Multiple regulatory roles of the carboxy terminus of Ste2p a yeast GPCR. Pharmacol Res 65(1): 31-40.
  4. Mullen, D. G., Kyro, K., Hauser, M., Gustavsson, M., Veglia, G., Becker, J. M., Naider, F. and Distefano, M. D. (2011). Synthesis of a-factor peptide from Saccharomyces cerevisiae and photoactive analogues via Fmoc solid phase methodology. Bioorg Med Chem 19(1): 490-497.
  5. Caroccia, K. E., Estephan, R., Cohen, L. S., Arshava, B., Hauser, M., Zerbe, O., Becker, J. M. and Naider, F. (2011). Expression and biophysical analysis of a triple-transmembrane domain-containing fragment from a yeast G protein-coupled receptor. Biopolymers 96(6): 757-771.
  6. Becker, J. M., Kauffman, S. J., Hauser, M., Huang, L., Lin, M., Sillaots, S., Jiang, B., Xu, D. and Roemer, T. (2010). Pathway analysis of Candida albicans survival and virulence determinants in a murine infection model. Proc Natl Acad Sci U S A 107(51): 22044-22049.
  7. Cohen, L. S., Arshava, B., Estephan, R., Englander, J., Hauser, M., Becker, J. M. and Naider, F. (2009). Expression of double transmembrane domain GPCR fragments for biophysical analysis. Adv Exp Med Biol 611: 303-304.
  8. Huang, L. Y., Umanah, G., Hauser, M., Son, C., Arshava, B., Naider, F. and Becker, J. M. (2008). Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. Biochemistry 47(20): 5638-5648.
  9. Cohen, L. S., Arshava, B., Estephan, R., Englander, J., Kim, H., Hauser, M., Zerbe, O., Ceruso, M., Becker, J. M. and Naider, F. (2008). Expression and biophysical analysis of two double-transmembrane domain-containing fragments from a yeast G protein-coupled receptor. Biopolymers 90(2): 117-130.
  10. Hauser, M., Kauffman, S., Lee, B. K., Naider, F. and Becker, J. M. (2007). The first extracellular loop of the Saccharomyces cerevisiae G protein-coupled receptor Ste2p undergoes a conformational change upon ligand binding. J Biol Chem 282(14): 10387-10397.
  11. Cai, H., Hauser, M., Naider, F. and Becker, J. M. (2007). Differential regulation and substrate preferences in two peptide transporters of Saccharomyces cerevisiae. Eukaryot Cell 6(10): 1805-1813.
  12. Vizcaino, J. A., Cardoza, R. E., Hauser, M., Hermosa, R., Rey, M., Llobell, A., Becker, J. M., Gutierrez, S. and Monte, E. (2006). ThPTR2, a di/tri-peptide transporter gene from Trichoderma harzianum. Fungal Genet Biol 43(4): 234-246.
  13. Janiak, A. M., Sargsyan, H., Russo, J., Naider, F., Hauser, M. and Becker, J. M. (2005). Functional expression of the Candida albicans alpha-factor receptor in Saccharomyces cerevisiae. Fungal Genet Biol 42(4): 328-338.
  14. Hauser, M., Kauffman, S., Naider, F. and Becker, J. M. (2005). Substrate preference is altered by mutations in the fifth transmembrane domain of Ptr2p, the di/tri-peptide transporter of Saccharomyces cerevisiae. Mol Membr Biol 22(3): 215-227.
  15. Lee, B. K., Lee, Y. H., Hauser, M., Son, C. D., Khare, S., Naider, F. and Becker, J. M. (2002). Tyr266 in the sixth transmembrane domain of the yeast alpha-factor receptor plays key roles in receptor activation and ligand specificity. Biochemistry 41(46): 13681-13689.
  16. Ding, F. X., Lee, B. K., Hauser, M., Patri, R., Arshava, B., Becker, J. M. and Naider, F. (2002). Study of the binding environment of alpha-factor in its G protein-coupled receptor using fluorescence spectroscopy. J Pept Res 60(1): 65-74.
  17. Wang, Z., Zheng, L., Liu, H., Wang, Q., Hauser, M., Kauffman, S., Becker, J. M. and Szaniszlo, P. J. (2001). WdChs2p, a class I chitin synthase, together with WdChs3p (class III) contributes to virulence in Wangiella (Exophiala) dermatitidis. Infect Immun 69(12): 7517-7526.
  18. Hauser, M., Narita, V., Donhardt, A. M., Naider, F. and Becker, J. M. (2001). Multiplicity and regulation of genes encoding peptide transporters in Saccharomyces cerevisiae. Mol Membr Biol 18(1): 105-112.
  19. Feng, B., Wang, X., Hauser, M., Kaufmann, S., Jentsch, S., Haase, G., Becker, J. M. and Szaniszlo, P. J. (2001). Molecular cloning and characterization of WdPKS1, a gene involved in dihydroxynaphthalene melanin biosynthesis and virulence in Wangiella (Exophiala) dermatitidis. Infect Immun 69(3): 1781-1794.
  20. Ding, F. X., Lee, B. K., Hauser, M., Davenport, L., Becker, J. M. and Naider, F. (2001). Probing the binding domain of the Saccharomyces cerevisiae alpha-mating factor receptor with rluorescent ligands. Biochemistry 40(4): 1102-1108.
  21. Hauser, M., Donhardt, A. M., Barnes, D., Naider, F. and Becker, J. M. (2000). Enkephalins are transported by a novel eukaryotic peptide uptake system. J Biol Chem 275(5): 3037-3041.
Protocols by Melinda Hauser
  1. Halo Assay for Toxic Peptides and Other Compounds in Microorganisms
  2. Uptake Assay for Radiolabeled Peptides in Yeast