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Thomas Hoenen

Education

Ph.D. in Human Biology, Department of Virology, Philipps University Marburg, Germany, 2007

Current position

Laboratory Head, Laboratory for Molecular Biology of Filoviruses, Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald – Isle of Riems, Germany

Publications (since 2012)

  1. Martin, B., Hoenen, T., Canard, B. and Decroly, E. (2016). Filovirus proteins for antiviral drug discovery: A structure/function analysis of surface glycoproteins and virus entry. Antiviral Res 135: 1-14.

  2. Rosenke, K., Adjemian, J., Munster, V. J., Marzi, A., Falzarano, D., Onyango, C. O., Ochieng, M., Juma, B., Fischer, R. J., Prescott, J. B., Safronetz, D., Omballa, V., Owuor, C., Hoenen, T., Groseth, A., Martellaro, C., van Doremalen, N., Zemtsova, G., Self, J., Bushmaker, T., McNally, K., Rowe, T., Emery, S. L., Feldmann, F., Williamson, B. N., Best, S. M., Nyenswah, T. G., Grolla, A., Strong, J. E., Kobinger, G., Bolay, F. K., Zoon, K. C., Stassijns, J., Giuliani, R., de Smet, M., Nichol, S. T., Fields, B., Sprecher, A., Massaquoi, M., Feldmann, H. and de Wit, E. (2016). Plasmodium Parasitemia Associated With Increased Survival in Ebola Virus-Infected Patients. Clin Infect Dis 63(8): 1026-1033.

  3. Hoenen, T., Groseth, A., Safronetz, D., Wollenberg, K. and Feldmann, H. (2016). Response to Comment on "Mutation rate and genotype variation of Ebola virus from Mali case sequences". Science 353(6300): 658.

  4. Nelson, E. A., Barnes, A. B., Wiehle, R. D., Fontenot, G. K., Hoenen, T. and White, J. M. (2016). Clomiphene and Its Isomers Block Ebola Virus Particle Entry and Infection with Similar Potency: Potential Therapeutic Implications. Viruses 8(8).

  5. de Wit, E., Kramer, S., Prescott, J., Rosenke, K., Falzarano, D., Marzi, A., Fischer, R. J., Safronetz, D., Hoenen, T., Groseth, A., van Doremalen, N., Bushmaker, T., McNally, K. L., Feldmann, F., Williamson, B. N., Best, S. M., Ebihara, H., Damiani, I. A., Adamson, B., Zoon, K. C., Nyenswah, T. G., Bolay, F. K., Massaquoi, M., Sprecher, A., Feldmann, H. and Munster, V. J. (2016). Clinical Chemistry of Patients With Ebola in Monrovia, Liberia. J Infect Dis 214(suppl 3): S303-S307.

  6. de Wit, E., Rosenke, K., Fischer, R. J., Marzi, A., Prescott, J., Bushmaker, T., van Doremalen, N., Emery, S. L., Falzarano, D., Feldmann, F., Groseth, A., Hoenen, T., Juma, B., McNally, K. L., Ochieng, M., Omballa, V., Onyango, C. O., Owuor, C., Rowe, T., Safronetz, D., Self, J., Williamson, B. N., Zemtsova, G., Grolla, A., Kobinger, G., Rayfield, M., Stroher, U., Strong, J. E., Best, S. M., Ebihara, H., Zoon, K. C., Nichol, S. T., Nyenswah, T. G., Bolay, F. K., Massaquoi, M., Feldmann, H. and Fields, B. (2016). Ebola Laboratory Response at the Eternal Love Winning Africa Campus, Monrovia, Liberia, 2014-2015. J Infect Dis 214(suppl 3): S169-S176.

  7. de Wit, E., Falzarano, D., Onyango, C., Rosenke, K., Marzi, A., Ochieng, M., Juma, B., Fischer, R. J., Prescott, J. B., Safronetz, D., Omballa, V., Owuor, C., Hoenen, T., Groseth, A., van Doremalen, N., Zemtsova, G., Self, J., Bushmaker, T., McNally, K., Rowe, T., Emery, S. L., Feldmann, F., Williamson, B., Nyenswah, T. G., Grolla, A., Strong, J. E., Kobinger, G., Stroeher, U., Rayfield, M., Bolay, F. K., Zoon, K. C., Stassijns, J., Tampellini, L., de Smet, M., Nichol, S. T., Fields, B., Sprecher, A., Feldmann, H., Massaquoi, M. and Munster, V. J. (2016). The Merits of Malaria Diagnostics during an Ebola Virus Disease Outbreak. Emerg Infect Dis 22(2): 323-326.

  8. Hoenen, T., Groseth, A., Rosenke, K., Fischer, R. J., Hoenen, A., Judson, S. D., Martellaro, C., Falzarano, D., Marzi, A., Squires, R. B., Wollenberg, K. R., de Wit, E., Prescott, J., Safronetz, D., van Doremalen, N., Bushmaker, T., Feldmann, F., McNally, K., Bolay, F. K., Fields, B., Sealy, T., Rayfield, M., Nichol, S. T., Zoon, K. C., Massaquoi, M., Munster, V. J. and Feldmann, H. (2016). Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool. Emerg Infect Dis 22(2): 331-334.

  9. McCarthy, S. D., Majchrzak-Kita, B., Racine, T., Kozlowski, H. N., Baker, D. P., Hoenen, T., Kobinger, G. P., Fish, E. N. and Branch, D. R. (2016). A Rapid Screening Assay Identifies Monotherapy with Interferon-ss and Combination Therapies with Nucleoside Analogs as Effective Inhibitors of Ebola Virus. PLoS Negl Trop Dis 10(1): e0004364.

  10. Gabriel, G., Feldmann, F., Reimer, R., Thiele, S., Fischer, M., Hartmann, E., Bader, M., Ebihara, H., Hoenen, T. and Feldmann, H. (2015). Importin-alpha7 Is Involved in the Formation of Ebola Virus Inclusion Bodies but Is Not Essential for Pathogenicity in Mice. J Infect Dis 212 Suppl 2: S316-321.

  11. Hoenen, T., Marzi, A., Scott, D. P., Feldmann, F., Callison, J., Safronetz, D., Ebihara, H. and Feldmann, H. (2015). Soluble Glycoprotein Is Not Required for Ebola Virus Virulence in Guinea Pigs. J Infect Dis 212 Suppl 2: S242-246.

  12. Tsuda, Y., Hoenen, T., Banadyga, L., Weisend, C., Ricklefs, S. M., Porcella, S. F. and Ebihara, H. (2015). An Improved Reverse Genetics System to Overcome Cell-Type-Dependent Ebola Virus Genome Plasticity. J Infect Dis 212 Suppl 2: S129-137.

  13. Dunham, E. C., Banadyga, L., Groseth, A., Chiramel, A. I., Best, S. M., Ebihara, H., Feldmann, H. and Hoenen, T. (2015). Assessing the contribution of interferon antagonism to the virulence of West African Ebola viruses. Nat Commun 6: 8000.

  14. Hoenen, T., Safronetz, D., Groseth, A., Wollenberg, K. R., Koita, O. A., Diarra, B., Fall, I. S., Haidara, F. C., Diallo, F., Sanogo, M., Sarro, Y. S., Kone, A., Togo, A. C., Traore, A., Kodio, M., Dosseh, A., Rosenke, K., de Wit, E., Feldmann, F., Ebihara, H., Munster, V. J., Zoon, K. C., Feldmann, H. and Sow, S. (2015). Virology. Mutation rate and genotype variation of Ebola virus from Mali case sequences. Science 348(6230): 117-119.

  15. Hoenen, T., Groseth, A., Feldmann, F., Marzi, A., Ebihara, H., Kobinger, G., Gunther, S. and Feldmann, H. (2014). Complete genome sequences of three ebola virus isolates from the 2014 outbreak in west Africa. Genome Announc 2(6).

  16. Brauburger, K., Boehmann, Y., Tsuda, Y., Hoenen, T., Olejnik, J., Schumann, M., Ebihara, H. and Muhlberger, E. (2014). Analysis of the highly diverse gene borders in Ebola virus reveals a distinct mechanism of transcriptional regulation. J Virol 88(21): 12558-12571.

  17. Hoenen, T. and Feldmann, H. (2014). Reverse genetics systems as tools for the development of novel therapies against filoviruses. Expert Rev Anti Infect Ther 12(10): 1253-1263.

  18. Hoenen, T., Watt, A., Mora, A. and Feldmann, H. (2014). Modeling the lifecycle of Ebola virus under biosafety level 2 conditions with virus-like particles containing tetracistronic minigenomes. J Vis Exp(91): 52381.

  19. Hoenen, T. and Feldmann, H. (2014). Ebolavirus in West Africa, and the use of experimental therapies or vaccines. BMC Biol 12: 80.

  20. Kuhn, J. H., Andersen, K. G., Bao, Y., Bavari, S., Becker, S., Bennett, R. S., Bergman, N. H., Blinkova, O., Bradfute, S., Brister, J. R., Bukreyev, A., Chandran, K., Chepurnov, A. A., Davey, R. A., Dietzgen, R. G., Doggett, N. A., Dolnik, O., Dye, J. M., Enterlein, S., Fenimore, P. W., Formenty, P., Freiberg, A. N., Garry, R. F., Garza, N. L., Gire, S. K., Gonzalez, J. P., Griffiths, A., Happi, C. T., Hensley, L. E., Herbert, A. S., Hevey, M. C., Hoenen, T., Honko, A. N., Ignatyev, G. M., Jahrling, P. B., Johnson, J. C., Johnson, K. M., Kindrachuk, J., Klenk, H. D., Kobinger, G., Kochel, T. J., Lackemeyer, M. G., Lackner, D. F., Leroy, E. M., Lever, M. S., Muhlberger, E., Netesov, S. V., Olinger, G. G., Omilabu, S. A., Palacios, G., Panchal, R. G., Park, D. J., Patterson, J. L., Paweska, J. T., Peters, C. J., Pettitt, J., Pitt, L., Radoshitzky, S. R., Ryabchikova, E. I., Saphire, E. O., Sabeti, P. C., Sealfon, R., Shestopalov, A. M., Smither, S. J., Sullivan, N. J., Swanepoel, R., Takada, A., Towner, J. S., van der Groen, G., Volchkov, V. E., Volchkova, V. A., Wahl-Jensen, V., Warren, T. K., Warfield, K. L., Weidmann, M. and Nichol, S. T. (2014). Filovirus RefSeq entries: evaluation and selection of filovirus type variants, type sequences, and names. Viruses 6(9): 3663-3682.

  21. Watt, A., Moukambi, F., Banadyga, L., Groseth, A., Callison, J., Herwig, A., Ebihara, H., Feldmann, H. and Hoenen, T. (2014). A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity. J Virol 88(18): 10511-10524.

  22. Kindrachuk, J., Wahl-Jensen, V., Safronetz, D., Trost, B., Hoenen, T., Arsenault, R., Feldmann, F., Traynor, D., Postnikova, E., Kusalik, A., Napper, S., Blaney, J. E., Feldmann, H. and Jahrling, P. B. (2014). Ebola virus modulates transforming growth factor beta signaling and cellular markers of mesenchyme-like transition in hepatocytes. J Virol 88(17): 9877-9892.

  23. Kuhn, J. H., Bao, Y., Bavari, S., Becker, S., Bradfute, S., Brauburger, K., Rodney Brister, J., Bukreyev, A. A., Cai, Y., Chandran, K., Davey, R. A., Dolnik, O., Dye, J. M., Enterlein, S., Gonzalez, J. P., Formenty, P., Freiberg, A. N., Hensley, L. E., Hoenen, T., Honko, A. N., Ignatyev, G. M., Jahrling, P. B., Johnson, K. M., Klenk, H. D., Kobinger, G., Lackemeyer, M. G., Leroy, E. M., Lever, M. S., Muhlberger, E., Netesov, S. V., Olinger, G. G., Palacios, G., Patterson, J. L., Paweska, J. T., Pitt, L., Radoshitzky, S. R., Ryabchikova, E. I., Saphire, E. O., Shestopalov, A. M., Smither, S. J., Sullivan, N. J., Swanepoel, R., Takada, A., Towner, J. S., van der Groen, G., Volchkov, V. E., Volchkova, V. A., Wahl-Jensen, V., Warren, T. K., Warfield, K. L., Weidmann, M. and Nichol, S. T. (2014). Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA. Arch Virol 159(5): 1229-1237.

  24. Falzarano, D., Groseth, A. and Hoenen, T. (2014). Development and application of reporter-expressing mononegaviruses: current challenges and perspectives. Antiviral Res 103: 78-87.

  25. Hoenen, T., Groseth, A., Callison, J., Takada, A. and Feldmann, H. (2013). A novel Ebola virus expressing luciferase allows for rapid and quantitative testing of antivirals. Antiviral Res 99(3): 207-213.

  26. Lennartz, F., Hoenen, T., Lehmann, M., Groseth, A. and Garten, W. (2013). The role of oligomerization for the biological functions of the arenavirus nucleoprotein. Arch Virol 158(9): 1895-1905.

  27. Biedenkopf, N., Hartlieb, B., Hoenen, T. and Becker, S. (2013). Phosphorylation of Ebola virus VP30 influences the composition of the viral nucleocapsid complex: impact on viral transcription and replication. J Biol Chem 288(16): 11165-11174.

  28. Mehedi, M., Hoenen, T., Robertson, S., Ricklefs, S., Dolan, M. A., Taylor, T., Falzarano, D., Ebihara, H., Porcella, S. F. and Feldmann, H. (2013). Ebola virus RNA editing depends on the primary editing site sequence and an upstream secondary structure. PLoS Pathog 9(10): e1003677.

  29. Shabman, R. S., Hoenen, T., Groseth, A., Jabado, O., Binning, J. M., Amarasinghe, G. K., Feldmann, H. and Basler, C. F. (2013). An upstream open reading frame modulates ebola virus polymerase translation and virus replication. PLoS Pathog 9(1): e1003147.

  30. Hoenen, T., Shabman, R. S., Groseth, A., Herwig, A., Weber, M., Schudt, G., Dolnik, O., Basler, C. F., Becker, S. and Feldmann, H. (2012). Inclusion bodies are a site of ebolavirus replication. J Virol 86(21): 11779-11788.

  31. Hoenen, T., Groseth, A. and Feldmann, H. (2012). Current ebola vaccines. Expert Opin Biol Ther 12(7): 859-872.

  32. Groseth, A., Marzi, A., Hoenen, T., Herwig, A., Gardner, D., Becker, S., Ebihara, H. and Feldmann, H. (2012). The Ebola virus glycoprotein contributes to but is not sufficient for virulence in vivo. PLoS Pathog 8(8): e1002847.

Protocols by Thomas Hoenen
  1. Sequencing of Ebola Virus Genomes Using Nanopore Technology