Welcome guest, Login | Register


Jerry E. Chipuk


Ph.D. in Pharmacology, Case Western Reserve University, Cleveland, 2002

Current position

Member, The Tisch Cancer Institute, Mount Sinai Medical Center, New York
Member, The Diabetes, Obesity, & Metabolism Institute, Mount Sinai Medical Center, New York
Frontiers in Molecular and Cellular Oncology, Associate Editor
Oncogenesis, Associate Editor
Cell Death & Disease, Editorial Board
Molecular & Cellular Oncology, Associate Editor
Oncotarget, Advisory Board for “Autophagy and Cell Death”
Associate Professor, Dept. of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York
Associate Professor, Dept. of Dermatology, Icahn School of Medicine at Mount Sinai, New York


  1. Rimmele, P., Liang, R., Bigarella, C. L., Kocabas, F., Xie, J., Serasinghe, M. N., Chipuk, J., Sadek, H., Zhang, C. C. and Ghaffari, S. (2015). Mitochondrial metabolism in hematopoietic stem cells requires functional FOXO3. EMBO Rep 16(9): 1164-1176.
  2. Follis, A. V., Llambi, F., Merritt, P., Chipuk, J. E., Green, D. R. and Kriwacki, R. W. (2015). Pin1-Induced Proline Isomerization in Cytosolic p53 Mediates BAX Activation and Apoptosis. Mol Cell 59(4): 677-684.
  3. Wieder, S. Y., Serasinghe, M. N., Sung, J. C., Choi, D. C., Birge, M. B., Yao, J. L., Bernstein, E., Celebi, J. T. and Chipuk, J. E. (2015). Activation of the Mitochondrial Fragmentation Protein DRP1 Correlates with BRAF(V600E) Melanoma. J Invest Dermatol 135(10): 2544-2547.
  4. de Moll, E. H., Fu, Y., Qian, Y., Perkins, S. H., Wieder, S., Gnjatic, S., Remark, R., Bernardo, S. G., Moskalenko, M., Yao, J., Ferringer, T., Chang, R., Chipuk, J., Horst, B. A., Birge, M. B., Phelps, R. G. and Saenger, Y. M. (2015). Immune biomarkers are more accurate in prediction of survival in ulcerated than in non-ulcerated primary melanomas. Cancer Immunol Immunother 64(9): 1193-1203.
  5. Serasinghe, M. N., Wieder, S. Y., Renault, T. T., Elkholi, R., Asciolla, J. J., Yao, J. L., Jabado, O., Hoehn, K., Kageyama, Y., Sesaki, H. and Chipuk, J. E. (2015). Mitochondrial division is requisite to RAS-induced transformation and targeted by oncogenic MAPK pathway inhibitors. Mol Cell 57(3): 521-536.
  6. Renault, T. T., Floros, K. V., Elkholi, R., Corrigan, K. A., Kushnareva, Y., Wieder, S. Y., Lindtner, C., Serasinghe, M. N., Asciolla, J. J., Buettner, C., Newmeyer, D. D. and Chipuk, J. E. (2015). Mitochondrial shape governs BAX-induced membrane permeabilization and apoptosis. Mol Cell 57(1): 69-82.
  7. Renault, T. T., Elkholi, R., Bharti, A. and Chipuk, J. E. (2014). B cell lymphoma-2 (BCL-2) homology domain 3 (BH3) mimetics demonstrate differential activities dependent upon the functional repertoire of pro- and anti-apoptotic BCL-2 family proteins. J Biol Chem 289(38): 26481-26491.
  8. Galluzzi, L., Bravo-San Pedro, J. M., Vitale, I., Aaronson, S. A., Abrams, J. M., Adam, D., Alnemri, E. S., Altucci, L., Andrews, D., Annicchiarico-Petruzzelli, M., Baehrecke, E. H., Bazan, N. G., Bertrand, M. J., Bianchi, K., Blagosklonny, M. V., Blomgren, K., Borner, C., Bredesen, D. E., Brenner, C., Campanella, M., Candi, E., Cecconi, F., Chan, F. K., Chandel, N. S., Cheng, E. H., Chipuk, J. E., Cidlowski, J. A., Ciechanover, A., Dawson, T. M., Dawson, V. L., De Laurenzi, V., De Maria, R., Debatin, K. M., Di Daniele, N., Dixit, V. M., Dynlacht, B. D., El-Deiry, W. S., Fimia, G. M., Flavell, R. A., Fulda, S., Garrido, C., Gougeon, M. L., Green, D. R., Gronemeyer, H., Hajnoczky, G., Hardwick, J. M., Hengartner, M. O., Ichijo, H., Joseph, B., Jost, P. J., Kaufmann, T., Kepp, O., Klionsky, D. J., Knight, R. A., Kumar, S., Lemasters, J. J., Levine, B., Linkermann, A., Lipton, S. A., Lockshin, R. A., Lopez-Otin, C., Lugli, E., Madeo, F., Malorni, W., Marine, J. C., Martin, S. J., Martinou, J. C., Medema, J. P., Meier, P., Melino, S., Mizushima, N., Moll, U., Munoz-Pinedo, C., Nunez, G., Oberst, A., Panaretakis, T., Penninger, J. M., Peter, M. E., Piacentini, M., Pinton, P., Prehn, J. H., Puthalakath, H., Rabinovich, G. A., Ravichandran, K. S., Rizzuto, R., Rodrigues, C. M., Rubinsztein, D. C., Rudel, T., Shi, Y., Simon, H. U., Stockwell, B. R., Szabadkai, G., Tait, S. W., Tang, H. L., Tavernarakis, N., Tsujimoto, Y., Vanden Berghe, T., Vandenabeele, P., Villunger, A., Wagner, E. F., Walczak, H., White, E., Wood, W. G., Yuan, J., Zakeri, Z., Zhivotovsky, B., Melino, G. and Kroemer, G. (2015). Essential versus accessory aspects of cell death: recommendations of the NCCD 2015. Cell Death Differ 22(1): 58-73.
  9. Chong, R. A., Wu, K., Spratt, D. E., Yang, Y., Lee, C., Nayak, J., Xu, M., Elkholi, R., Tappin, I., Li, J., Hurwitz, J., Brown, B. D., Chipuk, J. E., Chen, Z. J., Sanchez, R., Shaw, G. S., Huang, L. and Pan, Z. Q. (2014). Pivotal role for the ubiquitin Y59-E51 loop in lysine 48 polyubiquitination. Proc Natl Acad Sci U S A 111(23): 8434-8439.
  10. Zhang, X., Lv, H., Zhou, Q., Elkholi, R., Chipuk, J. E., Reddy, M. V., Reddy, E. P. and Gallo, J. M. (2014). Preclinical pharmacological evaluation of a novel multiple kinase inhibitor, ON123300, in brain tumor models. Mol Cancer Ther 13(5): 1105-1116.
  11. Serasinghe, M. N., Missert, D. J., Asciolla, J. J., Podgrabinska, S., Wieder, S. Y., Izadmehr, S., Belbin, G., Skobe, M. and Chipuk, J. E. (2015). Anti-apoptotic BCL-2 proteins govern cellular outcome following B-RAF(V600E) inhibition and can be targeted to reduce resistance. Oncogene 34(7): 857-867.
  12. Chipuk, J. E. (2015). BCL-2 proteins: melanoma lives on the edge. Oncoscience 2(9): 729-730.
  13. Lopez-Rivera, E., Jayaraman, P., Parikh, F., Davies, M. A., Ekmekcioglu, S., Izadmehr, S., Milton, D. R., Chipuk, J. E., Grimm, E. A., Estrada, Y., Aguirre-Ghiso, J. and Sikora, A. G. (2014). Inducible nitric oxide synthase drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2. Cancer Res 74(4): 1067-1078.
  14. Elkholi, R., Renault, T. T., Serasinghe, M. N. and Chipuk, J. E. (2014). Putting the pieces together: How is the mitochondrial pathway of apoptosis regulated in cancer and chemotherapy? Cancer Metab 2: 16.
  15. Renault, T. T. and Chipuk, J. E. (2014). Death upon a kiss: mitochondrial outer membrane composition and organelle communication govern sensitivity to BAK/BAX-dependent apoptosis. Chem Biol 21(1): 114-123.
  16. Elkholi, R. and Chipuk, J. E. (2014). How do I kill thee? Let me count the ways: p53 regulates PARP-1 dependent necrosis. Bioessays 36(1): 46-51.
  17. Renault, T. T., Floros, K. V. and Chipuk, J. E. (2013). BAK/BAX activation and cytochrome c release assays using isolated mitochondria. Methods 61(2): 146-155.
  18. Beverly, L. J., Howell, L. A., Hernandez-Corbacho, M., Casson, L., Chipuk, J. E. and Siskind, L. J. (2013). BAK activation is necessary and sufficient to drive ceramide synthase-dependent ceramide accumulation following inhibition of BCL2-like proteins. Biochem J 452(1): 111-119.
  19. Fink, M. Y. and Chipuk, J. E. (2013). Survival of HER2-Positive Breast Cancer Cells: Receptor Signaling to Apoptotic Control Centers. Genes Cancer 4(5-6): 187-195.
  20. Renault, T. T. and Chipuk, J. E. (2013). Getting away with murder: how does the BCL-2 family of proteins kill with immunity? Ann N Y Acad Sci 1285: 59-79.
  21. Renault, T. T. and Chipuk, J. E. (2013). Inter-organellar communication with mitochondria regulates both the intrinsic and extrinsic pathways of apoptosis. Commun Integr Biol 6(2): e22872.
  22. Follis, A. V., Chipuk, J. E., Fisher, J. C., Yun, M. K., Grace, C. R., Nourse, A., Baran, K., Ou, L., Min, L., White, S. W., Green, D. R. and Kriwacki, R. W. (2013). PUMA binding induces partial unfolding within BCL-xL to disrupt p53 binding and promote apoptosis. Nat Chem Biol 9(3): 163-168.
  23. Anvekar, R. A., Asciolla, J. J., Lopez-Rivera, E., Floros, K. V., Izadmehr, S., Elkholi, R., Belbin, G., Sikora, A. G. and Chipuk, J. E. (2012). Sensitization to the mitochondrial pathway of apoptosis augments melanoma tumor cell responses to conventional chemotherapeutic regimens. Cell Death Dis 3: e420.
  24. Asciolla, J. J., Renault, T. T. and Chipuk, J. E. (2012). Examining BCL-2 family function with large unilamellar vesicles. J Vis Exp(68).
  25. Greter, M., Helft, J., Chow, A., Hashimoto, D., Mortha, A., Agudo-Cantero, J., Bogunovic, M., Gautier, E. L., Miller, J., Leboeuf, M., Lu, G., Aloman, C., Brown, B. D., Pollard, J. W., Xiong, H., Randolph, G. J., Chipuk, J. E., Frenette, P. S. and Merad, M. (2012). GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells. Immunity 36(6): 1031-1046.
  26. Chipuk, J. E., McStay, G. P., Bharti, A., Kuwana, T., Clarke, C. J., Siskind, L. J., Obeid, L. M. and Green, D. R. (2012). Sphingolipid metabolism cooperates with BAK and BAX to promote the mitochondrial pathway of apoptosis. Cell 148(5): 988-1000.
  27. Garrison, S. P., Phillips, D. C., Jeffers, J. R., Chipuk, J. E., Parsons, M. J., Rehg, J. E., Opferman, J. T., Green, D. R. and Zambetti, G. P. (2012). Genetically defining the mechanism of Puma- and Bim-induced apoptosis. Cell Death Differ 19(4): 642-649.
  28. Du, H., Wolf, J., Schafer, B., Moldoveanu, T., Chipuk, J. E. and Kuwana, T. (2011). BH3 domains other than Bim and Bid can directly activate Bax/Bak. J Biol Chem 286(1): 491-501.
  29. Anvekar, R. A., Asciolla, J. J., Missert, D. J. and Chipuk, J. E. (2011). Born to be alive: a role for the BCL-2 family in melanoma tumor cell survival, apoptosis, and treatment. Front Oncol 1(34).
  30. Elkholi, R., Floros, K. V. and Chipuk, J. E. (2011). The Role of BH3-Only Proteins in Tumor Cell Development, Signaling, and Treatment. Genes Cancer 2(5): 523-537.
  31. McAuley, J. L., Chipuk, J. E., Boyd, K. L., Van De Velde, N., Green, D. R. and McCullers, J. A. (2010). PB1-F2 proteins from H5N1 and 20 century pandemic influenza viruses cause immunopathology. PLoS Pathog 6(7): e1001014.
  32. Chipuk, J. E., Moldoveanu, T., Llambi, F., Parsons, M. J. and Green, D. R. (2010). The BCL-2 family reunion. Mol Cell 37(3): 299-310.
  33. Schafer, B., Quispe, J., Choudhary, V., Chipuk, J. E., Ajero, T. G., Du, H., Schneiter, R. and Kuwana, T. (2009). Mitochondrial outer membrane proteins assist Bid in Bax-mediated lipidic pore formation. Mol Biol Cell 20(8): 2276-2285.
  34. Chipuk, J. E. and Green, D. R. (2009). PUMA cooperates with direct activator proteins to promote mitochondrial outer membrane permeabilization and apoptosis. Cell Cycle 8(17): 2692-2696.
  35. Chipuk, J. E., Fisher, J. C., Dillon, C. P., Kriwacki, R. W., Kuwana, T. and Green, D. R. (2008). Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proc Natl Acad Sci U S A 105(51): 20327-20332.
  36. Cassidy-Stone, A., Chipuk, J. E., Ingerman, E., Song, C., Yoo, C., Kuwana, T., Kurth, M. J., Shaw, J. T., Hinshaw, J. E., Green, D. R. and Nunnari, J. (2008). Chemical inhibition of the mitochondrial division dynamin reveals its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization. Dev Cell 14(2): 193-204.
  37. Chipuk, J. E. and Green, D. R. (2008). How do BCL-2 proteins induce mitochondrial outer membrane permeabilization? Trends Cell Biol 18(4): 157-164.
  38. Green, D. R. and Chipuk, J. E. (2008). Apoptosis: Stabbed in the BAX. Nature 455(7216): 1047-1049.
  39. Chipuk, J. E., Bouchier-Hayes, L. and Green, D. R. (2006). Mitochondrial outer membrane permeabilization during apoptosis: the innocent bystander scenario. Cell Death Differ 13(8): 1396-1402.
  40. Chipuk, J. E. and Green, D. R. (2006). Dissecting p53-dependent apoptosis. Cell Death Differ 13(6): 994-1002.
  41. Green, D. R. and Chipuk, J. E. (2006). p53 and metabolism: Inside the TIGAR. Cell 126(1): 30-32.
  42. Chipuk, J. E., Bouchier-Hayes, L., Kuwana, T., Newmeyer, D. D. and Green, D. R. (2005). PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science 309(5741): 1732-1735.
  43. Kuwana, T., Bouchier-Hayes, L., Chipuk, J. E., Bonzon, C., Sullivan, B. A., Green, D. R. and Newmeyer, D. D. (2005). BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly. Mol Cell 17(4): 525-535.
  44. Spierings, D., McStay, G., Saleh, M., Bender, C., Chipuk, J., Maurer, U. and Green, D. R. (2005). Connected to death: the (unexpurgated) mitochondrial pathway of apoptosis. Science 310(5745): 66-67.
  45. Chipuk, J. E. and Green, D. R. (2005). Do inducers of apoptosis trigger caspase-independent cell death? Nat Rev Mol Cell Biol 6(3): 268-275.
  46. Chipuk, J. E., Kuwana, T., Bouchier-Hayes, L., Droin, N. M., Newmeyer, D. D., Schuler, M. and Green, D. R. (2004). Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. Science 303(5660): 1010-1014.
  47. Chipuk, J. E. and Green, D. R. (2004). Cytoplasmic p53: bax and forward. Cell Cycle 3(4): 429-431.
  48. Chipuk, J. E., Maurer, U., Green, D. R. and Schuler, M. (2003). Pharmacologic activation of p53 elicits Bax-dependent apoptosis in the absence of transcription. Cancer Cell 4(5): 371-381.
  49. Chipuk, J. E. and Green, D. R. (2003). p53's believe it or not: lessons on transcription-independent death. J Clin Immunol 23(5): 355-361.
  50. Chipuk, J. E., Stewart, L. V., Ranieri, A., Song, K. and Danielpour, D. (2002). Identification and characterization of a novel rat ov-serpin family member, trespin. J Biol Chem 277(29): 26412-26421.
  51. Chipuk, J. E., Cornelius, S. C., Pultz, N. J., Jorgensen, J. S., Bonham, M. J., Kim, S. J. and Danielpour, D. (2002). The androgen receptor represses transforming growth factor-beta signaling through interaction with Smad3. J Biol Chem 277(2): 1240-1248.
  52. Chipuk, J. E., Bhat, M., Hsing, A. Y., Ma, J. and Danielpour, D. (2001). Bcl-xL blocks transforming growth factor-beta 1-induced apoptosis by inhibiting cytochrome c release and not by directly antagonizing Apaf-1-dependent caspase activation in prostate epithelial cells. J Biol Chem 276(28): 26614-26621.
Protocols by Jerry E. Chipuk
  1. Mouse Liver Mitochondria Isolation, Size Fractionation, and Real-time MOMP Measurement