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James B. Lok


Ph.D. in Entomology, Department of Entomology, Cornell University, 1981

Current Position

Professor of Parasitology, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, USA

Publications (since 2009)

  1. Lok J. B. and Unnasch T. R. Transgenesis in animal parasitic nematodes: Strongyloides spp. and Brugia spp. (September 30, 2013), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.163.1, http://www.wormbook.org.
  2. Pearce, E. J. and Lok, J. B. (2013). Imaging trematode and nematode parasites. Parasite Immunol 35(9-10): 248-255.
  3. Bonne-Annee, S., Kerepesi, L. A., Hess, J. A., O'Connell, A. E., Lok, J. B., Nolan, T. J. and Abraham, D. (2013). Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis. Infect Immun 81(9): 3346-3355.
  4. Massey, H. C., Jr., Ranjit, N., Stoltzfus, J. D. and Lok, J. B. (2013). Strongyloides stercoralis daf-2 encodes a divergent ortholog of Caenorhabditis elegans DAF-2. Int J Parasitol 43(7): 515-520.
  5. Lok, J. B. (2012). Nucleic acid transfection and transgenesis in parasitic nematodes. Parasitology 139(5): 574-588.
  6. Nolan, T. J. and Lok, J. B. (2012). Macrocyclic lactones in the treatment and control of parasitism in small companion animals. Curr Pharm Biotechnol 13(6): 1078-1094.
  7. Huang, S. C., Freitas, T. C., Amiel, E., Everts, B., Pearce, E. L., Lok, J. B. and Pearce, E. J. (2012). Fatty acid oxidation is essential for egg production by the parasitic flatworm Schistosoma mansoni. PLoS Pathog 8(10): e1002996.
  8. Stoltzfus, J. D., Minot, S., Berriman, M., Nolan, T. J. and Lok, J. B. (2012). RNAseq analysis of the parasitic nematode Strongyloides stercoralis reveals divergent regulation of canonical dauer pathways. PLoS Negl Trop Dis 6(10): e1854.
  9. Shao, H., Li, X., Nolan, T. J., Massey, H. C., Jr., Pearce, E. J. and Lok, J. B. (2012). Transposon-mediated chromosomal integration of transgenes in the parasitic nematode Strongyloides ratti and establishment of stable transgenic lines. PLoS Pathog 8(8): e1002871.
  10. Stoltzfus, J. D., Massey, H. C., Jr., Nolan, T. J., Griffith, S. D. and Lok, J. B. (2012). Strongyloides stercoralis age-1: a potential regulator of infective larval development in a parasitic nematode. PLoS One 7(6): e38587.
  11. Abraham, D., Hess, J. A., Mejia, R., Nolan, T. J., Lok, J. B., Lustigman, S. and Nutman, T. B. (2011). Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice. Vaccine 29(45): 8134-8140.
  12. Li, X., Shao, H., Junio, A., Nolan, T. J., Massey, H. C., Jr., Pearce, E. J., Viney, M. E. and Lok, J. B. (2011). Transgenesis in the parasitic nematode Strongyloides ratti. Mol Biochem Parasitol 179(2): 114-119.
  13. O'Connell, A. E., Hess, J. A., Santiago, G. A., Nolan, T. J., Lok, J. B., Lee, J. J. and Abraham, D. (2011). Major basic protein from eosinophils and myeloperoxidase from neutrophils are required for protective immunity to Strongyloides stercoralis in mice. Infect Immun 79(7): 2770-2778.
  14. Zhu, H., Li, J., Nolan, T. J., Schad, G. A. and Lok, J. B. (2011). Sensory neuroanatomy of Parastrongyloides trichosuri, a nematode parasite of mammals: Amphidial neurons of the first-stage larva. J Comp Neurol 519(12): 2493-2507.
  15. O'Connell, A. E., Redding, K. M., Hess, J. A., Lok, J. B., Nolan, T. J. and Abraham, D. (2011). Soluble extract from the nematode Strongyloides stercoralis induces CXCR2 dependent/IL-17 independent neutrophil recruitment. Microbes Infect 13(6): 536-544.
  16. Ramanathan, R., Varma, S., Ribeiro, J. M., Myers, T. G., Nolan, T. J., Abraham, D., Lok, J. B. and Nutman, T. B. (2011). Microarray-based analysis of differential gene expression between infective and noninfective larvae of Strongyloides stercoralis. PLoS Negl Trop Dis 5(5): e1039.
  17. Seiler, G. S., Nolan, T. J., Withnall, E., Reynolds, C., Lok, J. B. and Sleeper, M. M. (2010). Computed tomographic changes associated with the prepatent and early patent phase of dirofilariasis in an experimentally infected dog. Vet Radiol Ultrasound 51(2): 136-140.
  18. Hu, M., Lok, J. B., Ranjit, N., Massey, H. C., Jr., Sternberg, P. W. and Gasser, R. B. (2010). Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida). Int J Parasitol 40(4): 405-415.
  19. Lok, J. B. (2009). Transgenesis in parasitic nematodes: building a better array. Trends Parasitol 25(8): 345-347.
  20. Bowman, DD and LOK, JB (2009) Year-round heartworm prevention: two viewpoints. NAVC Clinician’s Brief  7: 25-27.
  21. Stein, L. H., Redding, K. M., Lee, J. J., Nolan, T. J., Schad, G. A., Lok, J. B. and Abraham, D. (2009). Eosinophils utilize multiple chemokine receptors for chemotaxis to the parasitic nematode Strongyloides stercoralis. J Innate Immun 1(6): 618-630.
  22. Wang, Z., Zhou, X. E., Motola, D. L., Gao, X., Suino-Powell, K., Conneely, A., Ogata, C., Sharma, K. K., Auchus, R. J., Lok, J. B., Hawdon, J. M., Kliewer, S. A., Xu, H. E. and Mangelsdorf, D. J. (2009). Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes. Proc Natl Acad Sci U S A 106(23): 9138-9143.
  23. Castelletto, M. L., Massey, H. C., Jr. and Lok, J. B. (2009). Morphogenesis of Strongyloides stercoralis infective larvae requires the DAF-16 ortholog FKTF-1. PLoS Pathog 5(4): e1000370.
Protocols by James B. Lok
  1. Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti