Welcome guest, Login | Register

Home


Michael W. Adams

Education

Ph.D. in Biochemistry, King's College, University of London, England, 1979

Current position

Professor in Biochemistry & Molecular Biology, Georgia Power Professor in Biotechnology, and Distinguished Research Professor, University of Georgia, Athens, GA 30602, USA

Publications (since 2005)

  1. Vaccaro, B. J., Thorgersen, M. P., Lancaster, W. A., Price, M. N., Wetmore, K. M., Poole, F. L., 2nd, Deutschbauer, A., Arkin, A. P. and Adams, M. W. (2016). Determining Roles of Accessory Genes in Denitrification by Mutant Fitness Analyses. Appl Environ Microbiol 82(1): 51-61.
  2. Schut, G. J., Lipscomb, G. L., Nguyen, D. M., Kelly, R. M. and Adams, M. W. (2016). Heterologous Production of an Energy-Conserving Carbon Monoxide Dehydrogenase Complex in the Hyperthermophile Pyrococcus furiosus. Front Microbiol 7: 29. In press.
  3. Rollin, J. A., Ye, X., Del Campo, J. M., Adams, M. W. and Zhang, Y. H. (2016). Novel Hydrogen Bioreactor and Detection Apparatus. Adv Biochem Eng Biotechnol 152: 35-51. [Epub ahead of print]
  4. Nguyen, D. M., Lipscomb, G. L., Schut, G. J., Vaccaro, B. J., Basen, M., Kelly, R. M. and Adams, M. W. (2016). Temperature-dependent acetoin production by Pyrococcus furiosus is catalyzed by a biosynthetic acetolactate synthase and its deletion improves ethanol production. Metab Eng 34: 71-79.
  5. Schut, G. J., Zadvornyy, O., Wu, C. H., Peters, J. W., Boyd, E. S. and Adams, M. W. (2016). The role of geochemistry and energetics in the evolution of modern respiratory complexes from a proton-reducing ancestor. Biochim Biophys Acta. In press.
  6. Peters, J. W., Miller, A. F., Jones, A. K., King, P. W. and Adams, M. W. (2016). Electron bifurcation. Curr Opin Chem Biol 31: 146-152. In press.
  7. Lancaster, W. A., Utturkar, S. M., Poole, F. L., Adams, M. W. W. and Brown, S. D. (2016). Draft genome sequence of Clostridium paradoxum strain JW-YL-7. J Bacteriol. In press.
  8. Greene, B. L., Wu, C. H., Vansuch, G. E., Adams, M. W. and Dyer, R. B. (2016). Proton Inventory and Dynamics in the Nia-S to Nia-C Transition of a [NiFe] Hydrogenase. Biochemistry 55(12): 1813-1825. In press.
  9. McTernan, P. M., Chandrayan, S. K., Wu, C. H., Vaccaro, B. J., Lancaster, W. A. and Adams, M. W. (2015). Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex. Protein Eng Des Sel 28(1): 1-8.
  10. Peters, J. W., Schut, G. J., Boyd, E. S., Mulder, D. W., Shepard, E. M., Broderick, J. B., King, P. W. and Adams, M. W. (2015). [FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation. Biochim Biophys Acta 1853(6): 1350-1369.
  11. Keller, M. W., Lipscomb, G. L., Loder, A. J., Schut, G. J., Kelly, R. M. and Adams, M. W. (2015). A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe. Metab Eng 27: 101-106.
  12. Keller, M. W., Loder, A., Basen, M., Izquierdo, I., Kelly, R. M. and Adams, M. W. W. (2015). Production of lignofuels and electrofuels by extremely thermophilic microbes. Biofuels 5: 499-515.
  13. Chandrayan, S. K., Wu, C. H., McTernan, P. M. and Adams, M. W. (2015). High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form. Protein Expr Purif 107: 90-94.
  14. Greene, B. L., Wu, C. H., Adams, M. W. W. and Dyer, R. B. (2015). Mechanism and bias of a [NiFe] hydrogenase revealed by photo-triggered nanosecond infrared spectroscopy. J Am Chem Soc 137: 4558-4566.
  15. Rollin, J. A., Martin del Campo, J., Myung, S., Sun, F., You, C., Bakovic, A., Castro, R., Chandrayan, S. K., Wu, C. H., Adams, M. W., Senger, R. S. and Zhang, Y. H. (2015). High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling. Proc Natl Acad Sci U S A 112(16): 4964-4969.
  16. Hawkins, A. B., Lian, H., Zeldes, B. M., Loder, A. J., Lipscomb, G. L., Schut, G. J., Keller, M. W., Adams, M. W. and Kelly, R. M. (2015). Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO(2)-based 3-hydroxypropionate production. Biotechnol Bioeng 112(8): 1533-1543.
  17. Thorgersen, M. P., Lancaster, W. A., Vaccaro, B. J., Poole, F. L., Rocha, A. M., Mehlhorn, T., Pettenato, A., Chakraborty, R., Hazen, T. C., Arkin, A. P. and Adams, M. W. W. (2015). Environmental molybdenum availability is key to nitrate removal in contaminated ground water environments. Appl Environ Microbiol 81: 4976-4983.
  18. Alahuhta, M., Taylor, L. E., 2nd, Brunecky, R., Sammond, D. W., Michener, W., Adams, M. W., Himmel, M. E., Bomble, Y. J. and Lunin, V. (2015). The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase. Acta Crystallogr D Biol Crystallogr 71(Pt 9): 1946-1954.
  19. Scott, I. M., Rubinstein, G. M., Lipscomb, G. L., Basen, M., Schut, G. J., Rhaesa, A. M., Lancaster, W. A., Poole, F. L., 2nd, Kelly, R. M. and Adams, M. W. (2015). A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria. Appl Environ Microbiol 81(20): 7339-7347.
  20. Wu, C. H., McTernan, P. M., Walter, M. E. and Adams, M. W. (2015). Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus. Archaea 2015: 912582.
  21. Loder, A. J., Zeldes, B. M., Garrison, G. D., 2nd, Lipscomb, G. L., Adams, M. W. and Kelly, R. M. (2015). Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes. Appl Environ Microbiol 81(20): 7187-7200.
  22. Kwan, P., McIntosh, C. L., Jennings, D. P., Hopkins, R. C., Chandrayan, S. K., Wu, C. H., Adams, M. W. and Jones, A. K. (2015). The [NiFe]-Hydrogenase of Pyrococcus furiosus Exhibits a New Type of Oxygen Tolerance. J Am Chem Soc 137(42): 13556-13565.
  23. Zeldes, B. M., Keller, M. W., Loder, A. J., Straub, C. T., Adams, M. W. and Kelly, R. M. (2015). Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals. Front Microbiol 6: 1209.
  24. Zurawski, J. V., Conway, J. M., Lee, L. L., Simpson, H. J., Izquierdo, J. A., Blumer-Schuette, S., Nookaew, I., Adams, M. W. and Kelly, R. M. (2015). Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization. Appl Environ Microbiol 81(20): 7159-7170.
  25. Lewis, D. L., Notey, J. S., Chandrayan, S. K., Loder, A. J., Lipscomb, G. L., Adams, M. W. and Kelly, R. M. (2015). A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology. Extremophiles 19(2): 269-281.
  26. Blumer-Schuette, S. E., Brown, S. D., Sander, K. B., Bayer, E. A., Kataeva, I., Zurawski, J. V., Conway, J. M., Adams, M. W. and Kelly, R. M. (2014). Thermophilic lignocellulose deconstruction. FEMS Microbiol Rev 38(3): 393-448.
  27. Basen, M., Rhaesa, A. M., Kataeva, I., Prybol, C. J., Scott, I. M., Poole, F. L. and Adams, M. W. (2014). Degradation of high loads of crystalline cellulose and of unpretreated plant biomass by the thermophilic bacterium Caldicellulosiruptor bescii. Bioresour Technol 152: 384-392.
  28. Lipscomb, G. L., Schut, G. J., Thorgersen, M. P., Nixon, W. J., Kelly, R. M. and Adams, M. W. (2014). Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane-bound complex. J Biol Chem 289(5): 2873-2879.
  29. Thorgersen, M. P., Lipscomb, G. L., Schut, G. J., Kelly, R. M. and Adams, M. W. (2014). Deletion of acetyl-CoA synthetases I and II increases production of 3-hydroxypropionate by the metabolically-engineered hyperthermophile Pyrococcus furiosus. Metab Eng 22: 83-88.
  30. Scott, J. W., Poole, F. L. and Adams, M. W. (2014). Characterization of ten heterotetrameric NDP-dependent acyl-CoA synthetases of the hyperthermophilic archaeon Pyrococcus furiosus. Archaea 2014: 176863.
  31. Hawkins, A. B., Adams, M. W. and Kelly, R. M. (2014). Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway. Appl Environ Microbiol 80(8): 2536-2545.
  32. Lancaster, W. A., Menon, A. L., Scott, I., Poole, F. L., Vaccaro, B. J., Thorgersen, M. P., Geller, J., Hazen, T. C., Hurt, R. A., Jr., Brown, S. D., Elias, D. A. and Adams, M. W. (2014). Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization. Metallomics 6(5): 1004-1013.
  33. Myung, S., Rollin, J., You, C., Sun, F., Chandrayan, S., Adams, M. W. and Zhang, Y. H. (2014). In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose. Metab Eng 24: 70-77.
  34. Esteves, A. M., Chandrayan, S. K., McTernan, P. M., Borges, N., Adams, M. W. and Santos, H. (2014). Mannosylglycerate and di-myo-inositol phosphate have interchangeable roles during adaptation of Pyrococcus furiosus to heat stress. Appl Environ Microbiol 80(14): 4226-4233.
  35. McTernan, P. M., Chandrayan, S. K., Wu, C. H., Vaccaro, B. J., Lancaster, W. A., Yang, Q., Fu, D., Hura, G. L., Tainer, J. A. and Adams, M. W. (2014). Intact functional fourteen-subunit respiratory membrane-bound [NiFe]-hydrogenase complex of the hyperthermophilic archaeon Pyrococcus furiosus. J Biol Chem 289(28): 19364-19372.
  36. Boyd, E., S., Schut, G. J., Adams, M. W. W. and Peters, J. W. (2014). Hydrogen metabolism and the evolution of biological respiration. Microbe 9: 361-367.
  37. Pratt, A. J., Shin, D. S., Merz, G. E., Rambo, R. P., Lancaster, W. A., Dyer, K. N., Borbat, P. P., Poole, F. L., 2nd, Adams, M. W., Freed, J. H., Crane, B. R., Tainer, J. A. and Getzoff, E. D. (2014). Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes. Proc Natl Acad Sci U S A 111(43): E4568-4576.
  38. Basen, M., Schut, G. J., Nguyen, D. M., Lipscomb, G. L., Benn, R. A., Prybol, C. J., Vaccaro, B. J., Poole, F. L., 2nd, Kelly, R. M. and Adams, M. W. (2014). Single gene insertion drives bioalcohol production by a thermophilic archaeon. Proc Natl Acad Sci U S A 111(49): 17618-17623.
  39. Brown, S. D., Utturkar, S. M., Magnuson, T. S., Ray, A. E., Poole, F. L., Lancaster, W. A., Thorgersen, M. P., Adams, M. W. and Elias, D. A. (2014). Complete Genome Sequence of Pelosinus sp. Strain UFO1 Assembled Using Single-Molecule Real-Time DNA Sequencing Technology. Genome Announc 2(5).
  40. Schut, G. J., Lipscomb, G. L., Han, Y., Notey, J. S., Kelly, R. M. and Adams, M. W. W. (2014). Order and Family Thermococcales. In: The Prokaryotes: Other Major Lineages of Bacteria and the Archaea. Springer-Verlag.
  41. Alahuhta, M., Brunecky, R., Chandrayan, P., Kataeva, I., Adams, M. W., Himmel, M. E. and Lunin, V. V. (2013). The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction. Acta Crystallogr D Biol Crystallogr 69(Pt 4): 534-539.
  42. Hawkins, A. S., Han, Y., Bennett, R. K., Adams, M. W. and Kelly, R. M. (2013). Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea. J Biol Chem 288(6): 4012-4022.
  43. Pang, W. L., Kaur, A., Ratushny, A. V., Cvetkovic, A., Kumar, S., Pan, M., Arkin, A. P., Aitchison, J. D., Adams, M. W. and Baliga, N. S. (2013). Metallochaperones regulate intracellular copper levels. PLoS Comput Biol 9(1): e1002880.
  44. Schut, G. J., Boyd, E. S., Peters, J. W. and Adams, M. W. (2013). The modular respiratory complexes involved in hydrogen and sulfur metabolism by heterotrophic hyperthermophilic archaea and their evolutionary implications. FEMS Microbiol Rev 37(2): 182-203.
  45. Kamali, S., Wang, H., Mitra, D., Ogata, H., Lubitz, W., Manor, B. C., Rauchfuss, T. B., Byrne, D., Bonnefoy, V., Jenney, F. E., Jr., Adams, M. W., Yoda, Y., Alp, E., Zhao, J. and Cramer, S. P. (2013). Observation of the Fe-CN and Fe-CO vibrations in the active site of [NiFe] hydrogenase by nuclear resonance vibrational spectroscopy. Angew Chem Int Ed Engl 52(2): 724-728.
  46. Hawkins, A. S., McTernan, P. M., Lian, H., Kelly, R. M. and Adams, M. W. (2013). Biological conversion of carbon dioxide and hydrogen into liquid fuels and industrial chemicals. Curr Opin Biotechnol 24(3): 376-384.
  47. Keller, M. W., Schut, G. J., Lipscomb, G. L., Menon, A. L., Iwuchukwu, I. J., Leuko, T. T., Thorgersen, M. P., Nixon, W. J., Hawkins, A. S., Kelly, R. M. and Adams, M. W. (2013). Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide. Proc Natl Acad Sci U S A 110(15): 5840-5845.
  48. Martin del Campo, J. S., Rollin, J., Myung, S., Chun, Y., Chandrayan, S., Patiño, R., Adams, M. W. and Zhang, Y. H. (2013). High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system. Angew Chem Int Ed Engl 52(17): 4587-4590.
  49. Zhu, Y., Kumar, S., Menon, A. L., Scott, R. A. and Adams, M. W. (2013). Regulation of iron metabolism by Pyrococcus furiosus. J Bacteriol 195(10): 2400-2407.
  50. Brunecky, R., Alahuhta, M., Xu, Q., Donohoe, B. S., Crowley, M. F., Kataeva, I. A., Yang, S. J., Resch, M. G., Adams, M. W., Lunin, V. V., Himmel, M. E. and Bomble, Y. J. (2013). Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA. Science 342(6165): 1513-1516.
  51. Kataeva, I., Foston, M., Yang, S. J., Pattathil, S., Biswal, A. K., Poole, F. L., Basen, M., Rhaesa, A. M., Thomas, T. P., Azidi, P., Olman, V., Safford, T. D., Mohler, K. E., Lewis, D. L., Doeppke, C., Zeng, Y., Tschaplinski, T., York, W. S., Davis, M., Mohnen, D., Xu, Y., Ragauskas, A., Ding, S. Y., Kelly, R. M., Hahn, M. G. and Adams, M. W. W. (2013). Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature. Energy Environ Sci 6: 2186-2195.
  52. Chandrayan, S. K., McTernan, P. M., Hopkins, R. C., Sun, J., Jenney, F. E., Jr. and Adams, M. W. (2012). Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase. J Biol Chem 287(5): 3257-3264.
  53. Basen, M., Sun, J. and Adams, M. W. (2012). Engineering a hyperthermophilic archaeon for temperature-dependent product formation. MBio 3(2): e00053-00012.
  54. Vaccaro, B. J., Menon, A. L., Lancaster, W. A. and Adams, M. W. W. (2012). Metallomics using Inductively Coupled Plasma Mass Spectrometry. Current Protocols in Chemical Biology 4: 249-274.
  55. Farkas, J., Stirrett, K., Lipscomb, G., Nixon, W., Scott, R. A., Adams, M. W. W. and Westpheling, J. (2012). The recombinogenic properties of the Pyrococcus furiosus COM1 strain enable rapid selection of targeted mutants. Appl Environ Microbiol 78: 4669-4676.
  56. Blumer-Schuette, S. E., Giannone, R. J., Zurawski, J. V., Ozdemir, I., Ma, Q., Yin, Y., Xu, Y., Kataeva, I., Poole, F. L., 2nd, Adams, M. W., Hamilton-Brehm, S. D., Elkins, J. G., Larimer, F. W., Land, M. L., Hauser, L. J., Cottingham, R. W., Hettich, R. L. and Kelly, R. M. (2012). Caldicellulosiruptor core and pangenomes reveal determinants for noncellulosomal thermophilic deconstruction of plant biomass. J Bacteriol 194(15): 4015-4028.
  57. Schut, G. J., Nixon, W. J., Lipscomb, G. L., Scott, R. A. and Adams, M. W. (2012). Mutational Analyses of the Enzymes Involved in the Metabolism of Hydrogen by the Hyperthermophilic Archaeon Pyrococcus furiosus. Front Microbiol 3: 163.
  58. Bridger, S. L., Lancaster, W. A., Poole, F. L., 2nd, Schut, G. J. and Adams, M. W. (2012). Genome sequencing of a genetically tractable Pyrococcus furiosus strain reveals a highly dynamic genome. J Bacteriol 194(15): 4097-4106.
  59. Han, Y., Hawkins, A. S., Adams, M. W. and Kelly, R. M. (2012). Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle. Appl Environ Microbiol 78(17): 6194-6202. T
  60. horgersen, M. P., Stirrett, K., Scott, R. A. and Adams, M. W. (2012). Mechanism of oxygen detoxification by the surprisingly oxygen-tolerant hyperthermophilic archaeon, Pyrococcus furiosus. Proc Natl Acad Sci U S A 109(45): 18547-18552.
  61. Guo, Y., Brecht, E., Aznavour, K., Nix, J. C., Xiao, Y., Wang, H., George, S. J., Bau, R., Keable, S., Peters, J. W., Adams, M. W. W., Jenney, F. E., Sturhahn, W., Alp, E. E., Zhao , J., Yoda, Y. and Cramer, S. P. (2012). Nuclear resonance vibrational spectroscopy (NRVS) of rubredoxin and MoFe protein crystals. Hyperfine Interact.
  62. de Crécy-Lagard, V., Phillips, G., Grochowski, L. L., El Yacoubi, B., Jenney, F., Adams, M. W., Murzin, A. G. and White, R. H. (2012). Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate. ACS Chem Biol 7(11): 1807-1816.
  63. Yannone, S. M., Hartung, S., Menon, A. L., Adams, M. W. and Tainer, J. A. (2012). Metals in biology: defining metalloproteomes. Curr Opin Biotechnol 23(1): 89-95.
  64. Dam, P., Kataeva, I., Yang, S. J., Zhou, F., Yin, Y., Chou, W., Poole, F. L., 2nd, Westpheling, J., Hettich, R., Giannone, R., Lewis, D. L., Kelly, R., Gilbert, H. J., Henrissat, B., Xu, Y. and Adams, M. W. (2011). Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Nucleic Acids Res 39(8): 3240-3254.
  65. Lipscomb, G. L., Stirrett, K., Schut, G. J., Yang, F., Jenney, F. E., Jr., Scott, R. A., Adams, M. W. and Westpheling, J. (2011). Natural competence in the hyperthermophilic archaeon Pyrococcus furiosus facilitates genetic manipulation: construction of markerless deletions of genes encoding the two cytoplasmic hydrogenases. Appl Environ Microbiol 77(7): 2232-2238.
  66. Lancaster, W. A., Praissman, J. L., Poole, F. L., 2nd, Cvetkovic, A., Menon, A. L., Scott, J. W., Jenney, F. E., Jr., Thorgersen, M. P., Kalisiak, E., Apon, J. V., Trauger, S. A., Siuzdak, G., Tainer, J. A. and Adams, M. W. (2011). A computational framework for proteome-wide pursuit and prediction of metalloproteins using ICP-MS and MS/MS data. BMC Bioinformatics 12: 64.
  67. Blumer-Schuette, S. E., Ozdemir, I., Mistry, D., Lucas, S., Lapidus, A., Cheng, J. F., Goodwin, L. A., Pitluck, S., Land, M. L., Hauser, L. J., Woyke, T., Mikhailova, N., Pati, A., Kyrpides, N. C., Ivanova, N., Detter, J. C., Walston-Davenport, K., Han, S., Adams, M. W. and Kelly, R. M. (2011). Complete genome sequences for the anaerobic, extremely thermophilic plant biomass-degrading bacteria Caldicellulosiruptor hydrothermalis, Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor kronotskyensis, Caldicellulosiruptor owensensis, and Caldice J Bacteriol 193(6): 1483-1484.
  68. Mitra, D., Pelmenschikov, V., Guo, Y., Case, D. A., Wang, H., Dong, W., Tan, M. L., Ichiye, T., Jenney, F. E., Adams, M. W., Yoda, Y., Zhao, J. and Cramer, S. P. (2011). Dynamics of the [4Fe-4S] cluster in Pyrococcus furiosus D14C ferredoxin via nuclear resonance vibrational and resonance Raman spectroscopies, force field simulations, and density functional theory calculations. Biochemistry 50(23): 5220-5235.
  69. Dillard, B. D., Demick, J. M., Adams, M. W. and Lanzilotta, W. N. (2011). A cryo-crystallographic time course for peroxide reduction by rubrerythrin from Pyrococcus furiosus. J Biol Inorg Chem 16(6): 949-959.
  70. Farkas, J., Chung, D., DeBarry, M., Adams, M. W. and Westpheling, J. (2011). Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector. Appl Environ Microbiol 77(18): 6343-6349.
  71. Yoon, S. H., Reiss, D. J., Bare, J. C., Tenenbaum, D., Pan, M., Slagel, J., Moritz, R. L., Lim, S., Hackett, M., Menon, A. L., Adams, M. W., Barnebey, A., Yannone, S. M., Leigh, J. A. and Baliga, N. S. (2011). Parallel evolution of transcriptome architecture during genome reorganization. Genome Res 21(11): 1892-1904.
  72. Alahuhta, M., Chandrayan, P., Kataeva, I., Adams, M. W., Himmel, M. E. and Lunin, V. V. (2011). A 1.5 A resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase. Acta Crystallogr Sect F Struct Biol Cryst Commun 67(Pt 12): 1498-1500.
  73. Bridger, S. L., Clarkson, S. M., Stirrett, K., DeBarry, M. B., Lipscomb, G. L., Schut, G. J., Westpheling, J., Scott, R. A. and Adams, M. W. (2011). Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus. J Bacteriol 193(23): 6498-6504.
  74. Hopkins, R. C., Sun, J., Jenney, F. E., Jr., Chandrayan, S. K., McTernan, P. M. and Adams, M. W. (2011). Homologous expression of a subcomplex of Pyrococcus furiosus hydrogenase that interacts with pyruvate ferredoxin oxidoreductase. PLoS One 6(10): e26569.
  75. Hawkins, A. S., Han, Y. J., Lian, H., Loder, A. J., Menon, A. L., Iwuchukwu, I. J., Keller, M., Leuko, T. T., Adams, M. W. W. and Kelly, R. M. (2011). Extremely thermophilic routes to microbial electrofuels. ACS Catalysis 1: 1043-1050.
  76. Holden, J. F., Menon, A. L. and Adams, M. W. W. (2011). Hyperthermophile-metal interactions in hydrothermal vent environments. In: Stolz, J. F. and Ormeland, R. S. (eds). Microbial Metal and Metalloid Metabolism: Advances and Applications. pp. 39-62, ASM Press, Washington, DC.
  77. Lancaster, W. A. and Adams, M. W. W. (2011). The influence of environment and metabolic capacity on the size of a microorganism. In: Luisi, P. L. and Stano, P. (eds). The Minimal Cell. pp. 93-104, Springer, New York.
  78. Strand, K. R., Sun, C., Li, T., Jenney, F. E., Jr., Schut, G. J. and Adams, M. W. (2010). Oxidative stress protection and the repair response to hydrogen peroxide in the hyperthermophilic archaeon Pyrococcus furiosus and in related species. Arch Microbiol 192(6): 447-459.
  79. Keese, A. M., Schut, G. J., Ouhammouch, M., Adams, M. W. and Thomm, M. (2010). Genome-wide identification of targets for the archaeal heat shock regulator phr by cell-free transcription of genomic DNA. J Bacteriol 192(5): 1292-1298.
  80. Clarkson, S. M., Newcomer, E. C., Young, E. G. and Adams, M. W. (2010). The elemental sulfur-responsive protein (SipA) from the hyperthermophilic archaeon Pyrococcus furiosus is regulated by sulfide in an iron-dependent manner. J Bacteriol 192(21): 5841-5843.
  81. Cvetkovic, A., Menon, A. L., Thorgersen, M. P., Scott, J. W., Poole, F. L., 2nd, Jenney, F. E., Jr., Lancaster, W. A., Praissman, J. L., Shanmukh, S., Vaccaro, B. J., Trauger, S. A., Kalisiak, E., Apon, J. V., Siuzdak, G., Yannone, S. M., Tainer, J. A. and Adams, M. W. (2010). Microbial metalloproteomes are largely uncharacterized. Nature 466(7307): 779-782.
  82. Yang, H., Lipscomb, G. L., Keese, A. M., Schut, G. J., Thomm, M., Adams, M. W., Wang, B. C. and Scott, R. A. (2010). SurR regulates hydrogen production in Pyrococcus furiosus by a sulfur-dependent redox switch. Mol Microbiol 77(5): 1111-1122.
  83. Sun, J., Hopkins, R. C., Jenney, F. E., McTernan, P. M. and Adams, M. W. (2010). Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production. PLoS One 5(5): e10526.
  84. Tonzetich, Z. J., Wang, H., Mitra, D., Tinberg, C. E., Do, L. H., Jenney, F. E., Jr., Adams, M. W., Cramer, S. P. and Lippard, S. J. (2010). Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy. J Am Chem Soc 132(20): 6914-6916.
Protocols by Michael W. Adams
  1. Nitrite Reduction Assay for Whole Pseudomonas Cells