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Ana Luísa Carvalho

Education

Ph.D. in Biochemistry (Molecular Biology), University of Coimbra, 1999

Current position

Tenured Assistant Professor, Department of Life Sciences, University of Coimbra; Group Leader, CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal (2004-present)

Publications (since 2006)

  1. Matos, C. A., Nobrega, C., Louros, S. R., Almeida, B., Ferreiro, E., Valero, J., Pereira de Almeida, L., Macedo-Ribeiro, S. and Carvalho, A. L. (2016). Ataxin-3 phosphorylation decreases neuronal defects in spinocerebellar ataxia type 3 models. J Cell Biol 212(4): 465-480.
  2. Vieira, M. M., Schmidt, J., Ferreira, J. S., She, K., Oku, S., Mele, M., Santos, A. E., Duarte, C. B., Craig, A. M. and Carvalho, A. L. (2016). Multiple domains in the C-terminus of NMDA receptor GluN2B subunit contribute to neuronal death following in vitro ischemia. Neurobiol Dis 89: 223-234.
  3. Almeida, B., Abreu, I. A., Matos, C. A., Fraga, J. S., Fernandes, S., Macedo, M. G., Gutierrez-Gallego, R., Pereira, P. J., Carvalho, A. L. and Macedo-Ribeiro, S. (2015). SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97. Biochim Biophys Acta 1852(9): 1950-1959.
  4. Ferreira, J. S., Schmidt, J., Rio, P., Aguas, R., Rooyakkers, A., Li, K. W., Smit, A. B., Craig, A. M. and Carvalho, A. L. (2015). GluN2B-Containing NMDA Receptors Regulate AMPA Receptor Traffic through Anchoring of the Synaptic Proteasome. J Neurosci 35(22): 8462-8479.
  5. Ribeiro, L. F., Catarino, T., Santos, S. D., Benoist, M., van Leeuwen, J. F., Esteban, J. A. and Carvalho, A. L. (2014). Ghrelin triggers the synaptic incorporation of AMPA receptors in the hippocampus. Proc Natl Acad Sci U S A 111(1): E149-158.
  6. Louros, S. R., Hooks, B. M., Litvina, L., Carvalho, A. L.* and Chen, C.* (2014). A role for stargazin in experience-dependent plasticity. Cell Rep 7(5): 1614-1625. (*Co-corresponding authors)
  7. Vieira, M., Fernandes, J., Carreto, L., Anuncibay-Soto, B., Santos, M., Han, J., Fernandez-Lopez, A., Duarte, C. B., Carvalho, A. L.* and Santos, A. E. (2014). Ischemic insults induce necroptotic cell death in hippocampal neurons through the up-regulation of endogenous RIP3. Neurobiol Dis 68: 26-36. (*Corresponding author)
  8. Ferreira, I. L., Ferreiro, E., Schmidt, J., Cardoso, J. M., Pereira, C. M., Carvalho, A. L., Oliveira, C. R. and Rego, A. C. (2015). Abeta and NMDAR activation cause mitochondrial dysfunction involving ER calcium release. Neurobiol Aging 36(2): 680-692.
  9. Castilho, A. F., Liberal, J. T., Baptista, F. I., Gaspar, J. M., Carvalho, A. L. and Ambrosio, A. F. (2014). Diabetes causes transient changes in the composition and phosphorylation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and interaction with auxiliary proteins in the rat retina. Mol Vis 20: 894-907.
  10. Fernandes, J., Vieira, M., Carreto, L., Santos, M. A., Duarte, C. B., Carvalho, A. L.* and Santos, A. E. (2014). In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons. PLoS One 9(6): e99958. (*Corresponding author)
  11. Mota, S. I., Ferreira, I. L., Valero, J., Ferreiro, E., Carvalho, A. L., Oliveira, C. R. and Rego, A. C. (2014). Impaired Src signaling and post-synaptic actin polymerization in Alzheimer's disease mice hippocampus--linking NMDA receptors and the reelin pathway. Exp Neurol 261: 698-709.
  12. Catarino, T., Ribeiro, L., Santos, S. D. and Carvalho, A. L. (2013). Regulation of synapse composition by protein acetylation: the role of acetylated cortactin. J Cell Sci 126(Pt 1): 149-162.
  13. She, K., Ferreira, J. S., Carvalho, A. L. and Craig, A. M. (2012). Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors. J Biol Chem 287(33): 27432-27445.
  14. Santos, S. D., Iuliano, O., Ribeiro, L., Veran, J., Ferreira, J. S., Rio, P., Mulle, C., Duarte, C. B. and Carvalho, A. L. (2012). Contactin-associated protein 1 (Caspr1) regulates the traffic and synaptic content of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors. J Biol Chem 287(9): 6868-6877.
  15. Matos, C. A., de Macedo-Ribeiro, S. and Carvalho, A. L. (2011). Polyglutamine diseases: the special case of ataxin-3 and Machado-Joseph disease. Prog Neurobiol 95(1): 26-48.
  16. Lobo, A. C., Gomes, J. R., Catarino, T., Mele, M., Fernandez, P., Inacio, A. R., Bahr, B. A., Santos, A. E., Wieloch, T., Carvalho, A. L. and Duarte, C. B. (2011). Cleavage of the vesicular glutamate transporters under excitotoxic conditions. Neurobiol Dis 44(3): 292-303.
  17. Ferreira, J. S., Rooyakkers, A., She, K., Ribeiro, L., Carvalho, A. L.* and Craig, A. M.* (2011). Activity and protein kinase C regulate synaptic accumulation of N-methyl-D-aspartate (NMDA) receptors independently of GluN1 splice variant. J Biol Chem 286(32): 28331-28342. (*Co-corresponding authors)
  18. Vieira, M., Fernandes, J., Burgeiro, A., Thomas, G. M., Huganir, R. L., Duarte, C. B., Carvalho, A. L. and Santos, A. E. (2010). Excitotoxicity through Ca2+-permeable AMPA receptors requires Ca2+-dependent JNK activation. Neurobiol Dis 40(3): 645-655.
  19. Moult, P. R., Cross, A., Santos, S. D., Carvalho, A. L., Lindsay, Y., Connolly, C. N., Irving, A. J., Leslie, N. R. and Harvey, J. (2010). Leptin regulates AMPA receptor trafficking via PTEN inhibition. J Neurosci 30(11): 4088-4101.
  20. Santos, S. D., Manadas, B., Duarte, C. B. and Carvalho, A. L. (2010). Proteomic analysis of an interactome for long-form AMPA receptor subunits. J Proteome Res 9(4): 1670-1682.
  21. Santos, S. D., Carvalho, A. L., Caldeira, M. V. and Duarte, C. B. (2009). Regulation of AMPA receptors and synaptic plasticity. Neuroscience 158(1): 105-125.
  22. Macedo-Ribeiro, S., Cortes, L., Maciel, P. and Carvalho, A. L. (2009). Nucleocytoplasmic shuttling activity of ataxin-3. PLoS One 4(6): e5834.
  23. Nunes-Correia, I., Rodriguez, J. M., Eulalio, A., Carvalho, A. L., Citovsky, V., Simoes, S., Faro, C., Salas, M. L. and Pedroso de Lima, M. C. (2008). African swine fever virus p10 protein exhibits nuclear import capacity and accumulates in the nucleus during viral infection. Vet Microbiol 130(1-2): 47-59.
  24. Gomes, A. R., Ferreira, J. S., Paternain, A. V., Lerma, J., Duarte, C. B. and Carvalho, A. L. (2008). Characterization of alternatively spliced isoforms of AMPA receptor subunits encoding truncated receptors. Mol Cell Neurosci 37(2): 323-334.
  25. Alves, S., Regulier, E., Nascimento-Ferreira, I., Hassig, R., Dufour, N., Koeppen, A., Carvalho, A. L., Simoes, S., de Lima, M. C., Brouillet, E., Gould, V. C., Deglon, N. and de Almeida, L. P. (2008). Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease. Hum Mol Genet 17(14): 2071-2083.
  26. Carvalho, A. L., Caldeira, M. V., Santos, S. D. and Duarte, C. B. (2008). Role of the brain-derived neurotrophic factor at glutamatergic synapses. Br J Pharmacol 153 Suppl 1: S310-324.
  27. Gomes, A. R., Correia, S. S., Esteban, J. A., Duarte, C. B. and Carvalho, A. L. (2007). PKC anchoring to GluR4 AMPA receptor subunit modulates PKC-driven receptor phosphorylation and surface expression. Traffic 8(3): 259-269.
  28. Ferro, A., Carvalho, A. L., Teixeira-Castro, A., Almeida, C., Tome, R. J., Cortes, L., Rodrigues, A. J., Logarinho, E., Sequeiros, J., Macedo-Ribeiro, S. and Maciel, P. (2007). NEDD8: a new ataxin-3 interactor. Biochim Biophys Acta 1773(11): 1619-1627.
  29. Caldeira, M. V., Melo, C. V., Pereira, D. B., Carvalho, R. F., Carvalho, A. L. and Duarte, C. B. (2007). BDNF regulates the expression and traffic of NMDA receptors in cultured hippocampal neurons. Mol Cell Neurosci 35(2): 208-219.
  30. Caldeira, M. V., Melo, C. V., Pereira, D. B., Carvalho, R., Correia, S. S., Backos, D. S., Carvalho, A. L., Esteban, J. A. and Duarte, C. B. (2007). Brain-derived neurotrophic factor regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons. J Biol Chem 282(17): 12619-12628.
  31. Caldeira, M. V., Melo, C. V., Pereira, D. B., Carvalho, R., Correia, S. S., Backos, D. S., Carvalho, A. L., Esteban, J. A. and Duarte, C. B. (2007). Brain-derived neurotrophic factor regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits in hippocampal neurons. J Biol Chem 282(17): 12619-12628.
  32. Alvaro, A. R., Rosmaninho-Salgado, J., Santiago, A. R., Martins, J., Aveleira, C., Santos, P. F., Pereira, T., Gouveia, D., Carvalho, A. L., Grouzmann, E., Ambrosio, A. F. and Cavadas, C. (2007). NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Muller cells. Neurochem Int 50(5): 757-763.
  33. Santos, A. E., Duarte, C. B., Iizuka, M., Barsoumian, E. L., Ham, J., Lopes, M. C., Carvalho, A. P. and Carvalho, A. L. (2006). Excitotoxicity mediated by Ca2+-permeable GluR4-containing AMPA receptors involves the AP-1 transcription factor. Cell Death Differ 13(4): 652-660..
  34. Eulalio, A., Nunes-Correia, I., Carvalho, A. L., Faro, C., Citovsky, V., Salas, J., Salas, M. L., Simoes, S. and de Lima, M. C. (2006). Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals. J Virol 80(3): 1393-1404.
  35. Cortes, L., Carvalho, A. L., Todo-Bom, A., Faro, C., Pires, E. and Verissimo, P. (2006). Purification of a novel aminopeptidase from the pollen of Parietaria judaica that alters epithelial integrity and degrades neuropeptides. J Allergy Clin Immunol 118(4): 878-884.
Protocols by Ana Luísa Carvalho
  1. Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons