Welcome guest, Sign in


Nicole R. Buan

Nicole R. Buan


Ph.D. in Microbiology, University of Wisconsin-Madison, Madison, 2005

Current position

Assistant Professor, Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USA (2010-present)


  1. Shea, M. T., Walter, M. E., Duszenko, N., Ducluzeau, A. L., Aldridge, J., King, S. K. and Buan, N. R. (2016). pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans. Plasmid 84-85: 27-35.
  2. Catlett, J. L., Ortiz, A. M. and Buan, N. R. (2015). Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production. Appl Environ Microbiol 81(19): 6528-6537.
  3. Lieber, D. J., Catlett, J., Madayiputhiya, N., Nandakumar, R., Lopez, M. M., Metcalf, W. W. and Buan, N. R. (2014). A multienzyme complex channels substrates and electrons through acetyl-CoA and methane biosynthesis pathways in Methanosarcina. PLoS One 9(9): e107563.
  4. Buan, N., Kulkarni, G. and Metcalf, W. (2011). Genetic methods for methanosarcina species. Methods Enzymol 494: 23-42.
  5. Buan, N. R. and Metcalf, W. W. (2010). Methanogenesis by Methanosarcina acetivorans involves two structurally and functionally distinct classes of heterodisulfide reductase. Mol Microbiol 75(4): 843-853.
  6. Escalante-Semerena, J.C., Woodson, J.D., Buan, N.R., and C.L. Zayas. 2009. Conversion of cobinamide into coenzyme B12. In: Warren M.J., Smith A.G., eds. Tetrapyrroles: Birth, Life and Death. Austin/New York: Landes Bioscience/Springer Science+Business Media, 2009:300-16.
  7. Ruiz-Sanchez, P., Mundwiler, S., Spingler, B., Buan, N. R., Escalante-Semerena, J. C. and Alberto, R. (2008). Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay. J Biol Inorg Chem 13(3): 335-347.
  8. Buan, N. R. and Escalante-Semerena, J. C. (2006). Purification and initial biochemical characterization of ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica. J Biol Chem 281(25): 16971-16977.
  9. Buan, N. R., Rehfeld, K. and Escalante-Semerena, J. C. (2006). Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1. J Bacteriol 188(10): 3543-3550.
  10. Buan, N. R. and Escalante-Semerena, J. C. (2005). Computer-assisted docking of flavodoxin with the ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein-protein interactions but not for catalysis. J Biol Chem 280(49): 40948-40956.
  11. Stich, T. A., Buan, N. R., Escalante-Semerena, J. C. and Brunold, T. C. (2005). Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J Am Chem Soc 127(24): 8710-8719.
  12. Stich, T. A., Buan, N. R. and Brunold, T. C. (2004). Spectroscopic and computational studies of Co2+corrinoids: spectral and electronic properties of the biologically relevant base-on and base-off forms of Co2+cobalamin. J Am Chem Soc 126(31): 9735-9749.
  13. Buan, N. R., Suh, S. J. and Escalante-Semerena, J. C. (2004). The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme. J Bacteriol 186(17): 5708-5714.
  14. Basha, E., Lee, G. J., Breci, L. A., Hausrath, A. C., Buan, N. R., Giese, K. C. and Vierling, E. (2004). The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions. J Biol Chem 279(9): 7566-7575.
  15. Friedrich, K. L., Giese, K. C., Buan, N. R.* and Vierling, E. (2004). Interactions between small heat shock protein subunits and substrate in small heat shock protein-substrate complexes. J Biol Chem 279(2): 1080-1089.
  16. Stich, T. A., Brooks, A. J., Buan, N. R. and Brunold, T. C. (2003). Spectroscopic and computational studies of Co3+-corrinoids: spectral and electronic properties of the B12 cofactors and biologically relevant precursors. J Am Chem Soc 125(19): 5897-5914.
  17. Fonseca, M. V., Buan, N. R., Horswill, A. R., Rayment, I. and Escalante-Semerena, J. C. (2002). The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct. J Biol Chem 277(36): 33127-33131.
Protocols by Nicole R. Buan
  1. Methods for Detecting Microbial Methane Production and Consumption by Gas Chromatography