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Blaine A. Pfeifer

Education

Ph.D., Department of Chemical and Biological Engineering, State University of New York at Buffalo, 904 Furnas Hall, Buffalo, NY 14260
Ph.D., Chemical Engineering, Department of Chemical and Biological Engineering, State University of New York at Buffalo, 2002

Current position

Associate Professor, SUNY-Buffalo, Chemical and Biological Engineering (2011-present)

Publications

  1. Ahmadi, M. K. and Pfeifer, B. A. (2016). Recent progress in therapeutic natural product biosynthesis using Escherichia coli. Curr Opin Biotechnol 42: 7-12.
  2. Beitelshees, M., Li, Y. and Pfeifer, B. A. (2016). Enhancing vaccine effectiveness with delivery technology. Curr Opin Biotechnol 42: 24-29.
  3. Kumpfmuller, J., Methling, K., Fang, L., Pfeifer, B. A., Lalk, M. and Schweder, T. (2016). Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis. Appl Microbiol Biotechnol 100(3): 1209-1220.
  4. Zhang, H., Fang, L., Osburne, M. S. and Pfeifer, B. A. (2016). The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis. Methods Mol Biol 1401: 121-134.
  5. Ahmadi, M. K., Fawaz, S., Fang, L., Yu, Z. and Pfeifer, B. A. (2015). Molecular variation of the nonribosomal peptide-polyketide siderophore yersiniabactin through biosynthetic and metabolic engineering. Biotechnol Bioeng.
  6. Jones, C. H., Hill, A., Chen, M. and Pfeifer, B. A. (2015). Contemporary approaches for nonviral gene therapy. Discov Med 19(107): 447-454.
  7. Jones, C. H., Chen, M., Gollakota, A., Ravikrishnan, A., Zhang, G., Lin, S., Tan, M., Cheng, C., Lin, H. and Pfeifer, B. A. (2015). Structure-Function Assessment of Mannosylated Poly(beta-amino esters) upon Targeted Antigen Presenting Cell Gene Delivery. Biomacromolecules 16(5): 1534-1541.
  8. Chung, T. C., Jones, C. H., Gollakota, A., Kamal Ahmadi, M., Rane, S., Zhang, G. and Pfeifer, B. A. (2015). Improved Escherichia coli Bactofection and Cytotoxicity by Heterologous Expression of Bacteriophage ΦX174 Lysis Gene E. Mol Pharm 12(5): 1691-1700.
  9. Jones, C. H., Gollakota, A., Chen, M., Chung, T. C., Ravikrishnan, A., Zhang, G. and Pfeifer, B. A. (2015). Influence of molecular weight upon mannosylated bio-synthetic hybrids for targeted antigen presenting cell gene delivery. Biomaterials 58: 103-111.
  10. Ahmadi, M. K., Fawaz, S., Jones, C. H., Zhang, G. and Pfeifer, B. A. (2015). Total Biosynthesis and Diverse Applications of the Nonribosomal Peptide-Polyketide Siderophore Yersiniabactin. Appl Environ Microbiol 81(16): 5290-5298.
  11. Zhang, G., Li, Y., Fang, L. and Pfeifer, B. A. (2015). Tailoring pathway modularity in the biosynthesis of erythromycin analogs heterologously engineered in E. coli. Sci Adv 1(4): e1500077.
  12. Ahmadi, M. K., Fawaz, S. and Pfeifer, B. A. (2015). An Aqueous Two Phase System to Pre-Purify a Heterologously-Produced Siderophore. Technology 1-4.
  13. Jones, C. H., Chen, M., Ravikrishnan, A., Reddinger, R., Zhang, G., Hakansson, A. P. and Pfeifer, B. A. (2015). Mannosylated poly (beta-amino esters) for targeted antigen presenting cell immune modulation. Biomaterials 37: 333-344.
  14. Li, Y., Zhang, G. and Pfeifer, B. A. (2015). Current and emerging options for taxol production. Adv Biochem Eng Biotechnol 148: 405-425.
  15. Jones, C. H., Chen, C. K., Chen, M., Ravikrishnan, A., Zhang, H., Gollakota, A., Chung, T., Cheng, C. and Pfeifer, B. A. (2015). PEGylated cationic polylactides for hybrid biosynthetic gene delivery. Mol Pharm 12(3): 846-856.
  16. Carter, K. A., Shao, S., Hoopes, M. I., Luo, D., Ahsan, B., Grigoryants, V. M., Song, W., Huang, H., Zhang, G., Pandey, R. K., Geng, J., Pfeifer, B. A., Scholes, C. P., Ortega, J., Karttunen, M. and Lovell, J. F. (2014). Porphyrin-phospholipid liposomes permeabilized by near-infrared light. Nat Commun 5: 3546.
  17. Chen, C. K., Wang, Q., Jones, C. H., Yu, Y., Zhang, H., Law, W. C., Lai, C. K., Zeng, Q., Prasad, P. N., Pfeifer, B. A. and Cheng, C. (2014). Synthesis of pH-responsive chitosan nanocapsules for the controlled delivery of doxorubicin. Langmuir 30(14): 4111-4119.
  18. Jones, C. H., Hakansson, A. P. and Pfeifer, B. A. (2014). Biomaterials at the Interface of Nano- and Micro-scale Vectorcellular Interactions in Genetic Vaccine Design. Journal of Materials Chemistry B 2:8053-8068.
  19. Li, Y. and Pfeifer, B. A. (2014). Heterologous production of plant-derived isoprenoid products in microbes and the application of metabolic engineering and synthetic biology. Curr Opin Plant Biol 19: 8-13.
  20. Jones, C. H., Ravikrishnan, A., Chen, M., Reddinger, R., Kamal Ahmadi, M., Rane, S., Hakansson, A. P. and Pfeifer, B. A. (2014). Hybrid biosynthetic gene therapy vector development and dual engineering capacity. Proc Natl Acad Sci U S A 111(34): 12360-12365.
  21. Jones, C. H., Chen, C. K., Jiang, M., Fang, L., Cheng, C. and Pfeifer, B. A. (2013). Synthesis of cationic polylactides with tunable charge densities as nanocarriers for effective gene delivery. Mol Pharm 10(3): 1138-1145.
  22. Jiang, M., Zhang, H. and Pfeifer, B. A. (2013). The logic, experimental steps, and potential of heterologous natural product biosynthesis featuring the complex antibiotic erythromycin A produced through E. coli. J Vis Exp(71): e4346.
  23. Rucker, J., Paul, J., Pfeifer, B. A. and Lee, K. (2013). Engineering E. coli for triglyceride accumulation through native and heterologous metabolic reactions. Appl Microbiol Biotechnol 97(6): 2753-2759.
  24. Jiang, M., Fang, L. and Pfeifer, B. A. (2013). Improved heterologous erythromycin A production through expression plasmid re-design. Biotechnol Prog 29(4): 862-869.
  25. Jiang, M. and Pfeifer, B. A. (2013). Metabolic and pathway engineering to influence native and altered erythromycin production through E. coli. Metab Eng 19: 42-49.
  26. Jiang, M., Zhang, H., Park, S. H., Li, Y. and Pfeifer, B. A. (2013). Deoxysugar pathway interchange for erythromycin analogues heterologously produced through Escherichia coli. Metab Eng 20: 92-100.
  27. Chen, C. K., Jones, C. H., Mistriotis, P., Yu, Y., Ma, X., Ravikrishnan, A., Jiang, M., Andreadis, S. T., Pfeifer, B. A. and Cheng, C. (2013). Poly(ethylene glycol)-block-cationic polylactide nanocomplexes of differing charge density for gene delivery. Biomaterials 34(37): 9688-9699.
  28. Jones, C. H., Rane, S., Patt, E., Ravikrishnan, A., Chen, C. K., Cheng, C. and Pfeifer, B. A. (2013). Polymyxin B treatment improves bactofection efficacy and reduces cytotoxicity. Mol Pharm 10(11): 4301-4308.
  29. Jones, C. H., Chen, C. K., Ravikrishnan, A., Rane, S. and Pfeifer, B. A. (2013). Overcoming nonviral gene delivery barriers: perspective and future. Mol Pharm 10(11): 4082-4098.
  30. Jiang, M., Stephanopoulos, G. and Pfeifer, B. A. (2012). Downstream reactions and engineering in the microbially reconstituted pathway for Taxol. Appl Microbiol Biotechnol 94(4): 841-849.
  31. Boghigian, B. A., Salas, D., Ajikumar, P. K., Stephanopoulos, G. and Pfeifer, B. A. (2012). Analysis of heterologous taxadiene production in K- and B-derived Escherichia coli. Appl Microbiol Biotechnol 93(4): 1651-1661.
  32. Zhang, H., Skalina, K., Jiang, M. and Pfeifer, B. A. (2012). Improved E. coli erythromycin A production through the application of metabolic and bioprocess engineering. Biotechnol Prog 28(1): 292-296.
  33. Boghigian, B. A., Armando, J., Salas, D. and Pfeifer, B. A. (2012). Computational identification of gene over-expression targets for metabolic engineering of taxadiene production. Appl Microbiol Biotechnol 93(5): 2063-2073.
  34. Armando, J. W., Boghigian, B. A. and Pfeifer, B. A. (2012). LC-MS/MS quantification of short-chain acyl-CoA's in Escherichia coli demonstrates versatile propionyl-CoA synthetase substrate specificity. Lett Appl Microbiol 54(2): 140-148.
  35. Jiang, M., Stephanopoulos, G. and Pfeifer, B. A. (2012). Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds. Appl Environ Microbiol 78(8): 2497-2504.
  36. Zhang, H., Boghigian, B. A., Armando, J. and Pfeifer, B. A. (2011). Methods and options for the heterologous production of complex natural products. Nat Prod Rep 28(1): 125-151.
  37. Boghigian, B. A., Zhang, H. and Pfeifer, B. A. (2011). Multi-factorial engineering of heterologous polyketide production in Escherichia coli reveals complex pathway interactions. Biotechnol Bioeng 108(6): 1360-1371.
  38. Boghigian, B. A., Myint, M., Wu, J. and Pfeifer, B. A. (2011). Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess. J Ind Microbiol Biotechnol 38(11): 1809-1820.
  39. Boghigian, B. A., Lee, K. and Pfeifer, B. A. (2010). Computational analysis of phenotypic space in heterologous polyketide biosynthesis--applications to Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. J Theor Biol 262(2): 197-207.
  40. Zhang, H., Boghigian, B. A. and Pfeifer, B. A. (2010). Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli. Biotechnol Bioeng 105(3): 567-573.
  41. Boghigian, B. A., Shi, H., Lee, K. and Pfeifer, B. A. (2010). Utilizing elementary mode analysis, pathway thermodynamics, and a genetic algorithm for metabolic flux determination and optimal metabolic network design. BMC Syst Biol 4: 49.
  42. Boghigian, B. A., Seth, G., Kiss, R. and Pfeifer, B. A. (2010). Metabolic flux analysis and pharmaceutical production. Metab Eng 12(2): 81-95.
  43. Wu, J., Boghigian, B. A., Myint, M., Zhang, H., Zhang, S. and Pfeifer, B. A. (2010). Construction and performance of heterologous polyketide-producing K-12- and B-derived Escherichia coli. Lett Appl Microbiol 51(2): 196-204.
  44. Ajikumar, P. K., Xiao, W. H., Tyo, K. E., Wang, Y., Simeon, F., Leonard, E., Mucha, O., Phon, T. H., Pfeifer, B. and Stephanopoulos, G. (2010). Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli. Science 330(6000): 70-74.
  45. Zhang, H., Wang, Y., Wu, J., Skalina, K. and Pfeifer, B. A. (2010). Complete biosynthesis of erythromycin A and designed analogs using E. coli as a heterologous host. Chem Biol 17(11): 1232-1240.
  46. Zhang, H., Wang, Y., Boghigian, B. and Pfeifer, B. A. (2009). Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli. Microb Biotechnol 2(3): 390-394.
  47. Pistorino, M. and Pfeifer, B. A. (2009). Efficient experimental design and micro-scale medium enhancement of 6-deoxyerythronolide B production through Escherichia coli. Biotechnol Prog 25(5): 1364-1371.
  48. Wang, Y. and Pfeifer, B. A. (2008). 6-deoxyerythronolide B production through chromosomal localization of the deoxyerythronolide B synthase genes in E. coli. Metab Eng 10(1): 33-38.
  49. Pistorino, M. and Pfeifer, B. A. (2008). Polyketide analysis using mass spectrometry, evaporative light scattering, and charged aerosol detector systems. Anal Bioanal Chem 390(4): 1189-1193.
  50. Zhang, H., Wang, Y. and Pfeifer, B. A. (2008). Bacterial hosts for natural product production. Mol Pharm 5(2): 212-225.
  51. Parsa, S., Wang, Y., Fuller, J., Langer, R. and Pfeifer, B. A. (2008). A comparison between polymeric microsphere and bacterial vectors for macrophage P388D1 gene delivery. Pharm Res 25(5): 1202-1208.
  52. Parsa, S., Wang, Y., Rines, K. and Pfeifer, B. A. (2008). A high-throughput comparison of recombinant gene expression parameters for E. coli-mediated gene transfer to P388D1 macrophage cells. J Biotechnol 137(1-4): 59-64.
  53. Boghigian, B. A. and Pfeifer, B. A. (2008). Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli. Biotechnol Lett 30(8): 1323-1330.
  54. Wang, Y., Boghigian, B. A. and Pfeifer, B. A. (2007). Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene. Appl Microbiol Biotechnol 77(2): 367-373.
  55. Parsa, S. and Pfeifer, B. (2007). Engineering bacterial vectors for delivery of genes and proteins to antigen-presenting cells. Mol Pharm 4(1): 4-17.
  56. Pfeifer, B. A., Burdick, J. A. and Langer, R. (2005). Formulation and surface modification of poly(ester-anhydride) micro- and nanospheres. Biomaterials 26(2): 117-124.
  57. Pfeifer, B. A., Burdick, J. A., Little, S. R. and Langer, R. (2005). Poly(ester-anhydride):poly(β-amino ester) micro- and nanospheres: DNA encapsulation and cellular transfection. Int J Pharm 304(1-2): 210-219.
  58. Hu, Z., Pfeifer, B. A., Chao, E., Murli, S., Kealey, J., Carney, J. R., Ashley, G., Khosla, C. and Hutchinson, C. R. (2003). A specific role of the Saccharopolyspora erythraea thioesterase II gene in the function of modular polyketide synthases. Microbiology 149(Pt 8): 2213-2225.
  59. Kinoshita, K., Pfeifer, B. A., Khosla, C. and Cane, D. E. (2003). Precursor-Directed polyketide biosynthesis in Escherichia coli. Bioorg Med Chem Lett 13(21): 3701-3704.
  60. Pfeifer, B. A., Wang, C. C., Walsh, C. T. and Khosla, C. (2003). Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host. Appl Environ Microbiol 69(11): 6698-6702.
  61. Dayem, L. C., Carney, J. R., Santi, D. V., Pfeifer, B. A., Khosla, C. and Kealey, J. T. (2002). Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli. Biochemistry 41(16): 5193-5201.
  62. Pfeifer, B., Hu, Z., Licari, P. and Khosla, C. (2002). Process and metabolic strategies for improved production of Escherichia coli-derived 6-deoxyerythronolide B. Appl Environ Microbiol 68(7): 3287-3292.
  63. Lombo, F., Pfeifer, B., Leaf, T., Ou, S., Kim, Y. S., Cane, D. E., Licari, P. and Khosla, C. (2001). Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering. Biotechnol Prog 17(4): 612-617.
  64. Pfeifer, B. A. and Khosla, C. (2001). Biosynthesis of polyketides in heterologous hosts. Microbiol Mol Biol Rev 65(1): 106-118.
  65. Pfeifer, B. A., Admiraal, S. J., Gramajo, H., Cane, D. E. and Khosla, C. (2001). Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science 291(5509): 1790-1792.
Protocols by Blaine A. Pfeifer
  1. Rust Removal Experiments