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Kazuhito Tomizawa


Ph.D. in Physiology, Kagawa Medical University School of Medicine, 1995

Current position

Professor, Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
Guest Professor, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan (2008-present)



  1. Han, X. J. and Tomizawa, K. (2011). Protein delivery. In: Lakshmipathy, U. and Thyagarajan, B. (eds). Primary and stem cells. Wiley, 119-133.
  2. Wei, F. Y. and Tomizawa, K. (2008). Cdk5 in Presynapses. In: Iq, N. Y., and Tsai, L. H. (eds). Cyclin dependent kinase 5 (Cdk5). Springer, 25-34.

Review articles (since 2003)

  1. Kaitsuka, T. and Tomizawa, K. (2015). Cell-penetrating peptide as a means of directing the differentiation of induced-pluripotent stem cells. Int J Mol Sci 16(11): 26667-26676.
  2. Watanabe, S., Wei, F. Y. and Tomizawa, K. (2013). Functional characterization of Cdkal1, a risk factor of type 2 diabetes, and the translational opportunities. Drug Discov Today 10, 65-68.
  3. Wei, F. Y. and Tomizawa, K. (2012). Development of type 2 diabetes caused by a deficiency of a tRNA(lys) modification. Islets 4(1): 71-73.
  4. Matsuzaki, M., Matsushita, H., Tomizawa, K. and Matsui, H. (2012). Oxytocin: a therapeutic target for mental disorders. J Physiol Sci 62(6): 441-444.
  5. Wei, F. Y. and Tomizawa, K. (2011). Functional loss of Cdkal1, a novel tRNA modification enzyme, causes the development of type 2 diabetes. Endocr J 58(10): 819-825.
  6. Feng, B., Matsui, H. and Tomizawa, K. (2011). Nanoparticle-based drug delivery systems for solid brain tumors. Curr Nanosci 7, 47-54.
  7. Han, X. J., Tomizawa, K., Fujimura, A., Ohmori, I., Nishiki, T., Matsushita, M. and Matsui, H. (2011). Regulation of mitochondrial dynamics and neurodegenerative diseases. Acta Med Okayama 65(1): 1-10.
  8. Wu, Y., Matsui, H. and Tomizawa, K. (2009). Amphiphysin I and regulation of synaptic vesicle endocytosis. Acta Med Okayama 63(6): 305-323.
  9. Ogawa, T., Ono, S., Ichikawa, T., Arimitsu, S., Onoda, K., Tokunaga, K., Sugiu, K., Tomizawa, K., Matsui, H. and Date, I. (2009). Protein transduction method for cerebrovascular disorders. Acta Med Okayama 63(1): 1-7.
  10. Wei, F. Y. and Tomizawa, K. (2007). Cyclin-dependent kinase 5 (Cdk5): a potential therapeutic target for the treatment of neurodegenerative diseases and diabetes mellitus. Mini Rev Med Chem 7(10): 1070-1074.
  11. Wu, H. Y., Tomizawa, K. and Matsui, H. (2007). Calpain-calcineurin signaling in the pathogenesis of calcium-dependent disorder. Acta Med Okayama 61(3): 123-137.
  12. Wu, H. Y., Matsui, H. and Tomizawa, K. (2005). Crosstalk between calpain and calcineurin in excitotoxic neurodegeneration; Therapeutic targets for the treatment of excitotoxic neurodegeneration. Curr Med Chem- Central Nervous System Agents 5, 207-216.
  13. Matsui, H., Tomizawa, K., Lu, Y. F. and Matsushita, M. (2003). Protein Therapy: in vivo protein transduction by polyarginine (11R) PTD and subcellular targeting delivery. Curr Protein Pept Sci 4(2): 151-157.

Peer-reviewed papers (since 1999)

  1. Wei, F. Y., Zhou, B., Suzuki, T., Miyata, K., Ujihara, Y., Horiguchi, H., Takahashi, N., Xie, P., Michiue, H., Fujimura, A., Kaitsuka, T., Matsui, H., Koga, Y., Mohri, S., Suzuki, T., Oike, Y. and Tomizawa, K. (2015). Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans. Cell Metab 21(3): 428-442.
  2. Yamada, H., Kikuchi, T., Masumoto, T., Wei, F. Y., Abe, T., Takeda, T., Nishiki, T., Tomizawa, K., Watanabe, M., Matsui, H. and Takei, K. (2015). Possible role of cortactin phosphorylation by protein kinase Calpha in actin-bundle formation at growth cone. Biol Cell 107(9): 319-330.
  3. Locke, J. M., Wei, F. Y., Tomizawa, K., Weedon, M. N. and Harries, L. W. (2015). A cautionary tale: the non-causal association between type 2 diabetes risk SNP, rs7756992, and levels of non-coding RNA, CDKAL1-v1. Diabetologia 58(4): 745-748.
  4. Kaitsuka, T., Katagiri, C., Beesetty, P., Nakamura, K., Hourani, S., Tomizawa, K., Kozak, J. A. and Matsushita, M. (2014). Inactivation of TRPM7 kinase activity does not impair its channel function in mice. Sci Rep 4: 5718.
  5. Zhou, B., Wei, F. Y., Kanai, N., Fujimura, A., Kaitsuka, T. and Tomizawa, K. (2014). Identification of a splicing variant that regulates type 2 diabetes risk factor CDKAL1 level by a coding-independent mechanism in human. Hum Mol Genet 23(17): 4639-4650.
  6. Yoshizawa, T., Karim, M. F., Sato, Y., Senokuchi, T., Miyata, K., Fukuda, T., Go, C., Tasaki, M., Uchimura, K., Kadomatsu, T., Tian, Z., Smolka, C., Sawa, T., Takeya, M., Tomizawa, K., Ando, Y., Araki, E., Akaike, T., Braun, T., Oike, Y., Bober, E. and Yamagata, K. (2014). SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway. Cell Metab 19(4): 712-721.
  7. Gotanda, Y., Wei, F. Y., Harada, H., Ohta, K., Nakamura, K., Tomizawa, K.* and Ushijima, K. (2014). Efficient transduction of 11 poly-arginine peptide in an ischemic lesion of mouse brain. J Stroke Cerebrovasc Dis 23(8): 2023-2030.
  8. Hakim, F., Kaitsuka, T., Raeed, J. M., Wei, F. Y., Shiraki, N., Akagi, T., Yokota, T., Kume, S. and Tomizawa, K. (2014). High oxygen condition facilitates the differentiation of mouse and human pluripotent stem cells into pancreatic progenitors and insulin-producing cells. J Biol Chem 289(14): 9623-9638.
  9. Michiue, H., Sakurai, Y., Kondo, N., Kitamatsu, M., Bin, F., Nakajima, K., Hirota, Y., Kawabata, S., Nishiki, T., Ohmori, I., Tomizawa, K., Miyatake, S., Ono, K. and Matsui, H. (2014). The acceleration of boron neutron capture therapy using multi-linked mercaptoundecahydrododecaborate (BSH) fused cell-penetrating peptide. Biomaterials 35(10): 3396-3405.
  10. Kaitsuka, T., Noguchi, H., Shiraki, N., Kubo, T., Wei, F. Y., Hakim, F., Kume, S. and Tomizawa, K. (2014). Generation of functional insulin-producing cells from mouse embryonic stem cells through 804G cell-derived extracellular matrix and protein transduction of transcription factors. Stem Cells Transl Med 3(1): 114-127.
  11. Xie, P., Wei, F. Y., Hirata, S., Kaitsuka, T., Suzuki, T., Suzuki, T. and Tomizawa, K. (2013). Quantitative PCR measurement of tRNA 2-methylthio modification for assessing type 2 diabetes risk. Clin Chem 59(11): 1604-1612.
  12. Fujimura, A., Michiue, H., Cheng, Y., Uneda, A., Tani, Y., Nishiki, T., Ichikawa, T., Wei, F. Y., Tomizawa, K. and Matsui, H. (2013). Cyclin G2 promotes hypoxia-driven local invasion of glioblastoma by orchestrating cytoskeletal dynamics. Neoplasia 15(11): 1272-1281.
  13. Han, X. J., Sun, L. F., Nishiyama, Y., Feng, B., Michiue, H., Seno, M., Matsui, H. and Tomizawa, K. (2013). Theranostic protein targeting ErbB2 for bioluminescence imaging and therapy for cancer. PLoS One 8(9): e75288.
  14. Karim, M. F., Yoshizawa, T., Sato, Y., Sawa, T., Tomizawa, K., Akaike, T. and Yamagata, K. (2013). Inhibition of H3K18 deacetylation of Sirt7 by Myb-binding protein 1a (Mybbp1a). Biochem Biophys Res Commun 441(1): 157-163.
  15. Yamada, H., Abe, T., Satoh, A., Okazaki, N., Tago, S., Kobayashi, K., Yoshida, Y., Oda, Y., Watanabe, M., Tomizawa, K., Matsui, H. and Takei, K. (2013). Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia. J Neurosci 33(10): 4514-4526.
  16. Ueda, Y., Wei, F. Y., Hide, T., Michiue, H., Takayama, K., Kaitsuka, T., Nakamura, H., Makino, K., Kuratsu, J., Futaki, S. and Tomizawa, K. (2012). Induction of autophagic cell death of glioma-initiating cells by cell-penetrating D-isomer peptides consisting of Pas and the p53 C-terminus. Biomaterials 33(35): 9061-9069.
  17. Candan, G., Michiue, H., Ishikawa, S., Fujimura, A., Hayashi, K., Uneda, A., Mori, A., Ohmori, I., Nishiki, T., Matsui, H. and Tomizawa, K. (2012). Combining poly-arginine with the hydrophobic counter-anion 4-(1-pyrenyl)-butyric acid for protein transduction in transdermal delivery. Biomaterials 33(27): 6468-6475.
  18. Sato, Y., Hatta, M., Karim, M. F., Sawa, T., Wei, F. Y., Sato, S., Magnuson, M. A., Gonzalez, F. J., Tomizawa, K., Akaike, T., Yoshizawa, T. and Yamagata, K. (2012). Anks4b, a novel target of HNF4alpha protein, interacts with GRP78 protein and regulates endoplasmic reticulum stress-induced apoptosis in pancreatic beta-cells. J Biol Chem 287(27): 23236-23245.
  19. Hitsuda, T., Michiue, H., Kitamatsu, M., Fujimura, A., Wang, F., Yamamoto, T., Han, X. J., Tazawa, H., Uneda, A., Ohmori, I., Nishiki, T., Tomizawa, K. and Matsui, H. (2012). A protein transduction method using oligo-arginine (3R) for the delivery of transcription factors into cell nuclei. Biomaterials 33(18): 4665-4672.
  20. Okimoto, N., Bosch, O. J., Slattery, D. A., Pflaum, K., Matsushita, H., Wei, F. Y., Ohmori, M., Nishiki, T., Ohmori, I., Hiramatsu, Y., Matsui, H., Neumann, I. D. and Tomizawa, K. (2012). RGS2 mediates the anxiolytic effect of oxytocin. Brain Res 1453: 26-33.
  21. Matsushita, H., Matsuzaki, M., Han, X. J., Nishiki, T. I., Ohmori, I., Michiue, H., Matsui, H. and Tomizawa, K. (2012). Antidepressant-like effect of sildenafil through oxytocin-dependent cyclic AMP response element-binding protein phosphorylation. Neuroscience 200: 13-18.
  22. Masumoto, T., Suzuki, K., Ohmori, I., Michiue, H., Tomizawa, K., Fujimura, A., Nishiki, T. and Matsui, H. (2012). Ca(2+)-independent syntaxin binding to the C(2)B effector region of synaptotagmin. Mol Cell Neurosci 49(1): 1-8.
  23. Wei, F. Y., Suzuki, T., Watanabe, S., Kimura, S., Kaitsuka, T., Fujimura, A., Matsui, H., Atta, M., Michiue, H., Fontecave, M., Yamagata, K., Suzuki, T. and Tomizawa, K. (2011). Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice. J Clin Invest 121(9): 3598-3608.
  24. Miwa, T., Minoda, R., Kaitsuka, T., Ise, M., Tomizawa, K. and Yumoto, E. (2011). Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides. Neuroreport 22(18): 994-999. *On cover page.
  25. Masumoto, T., Suzuki, K., Ohmori, I., Michiue, H., Tomizawa, K., Fujimura, A., Nishiki, T. and Matsui, H. (2012). Ca(2+)-independent syntaxin binding to the C(2)B effector region of synaptotagmin. Mol Cell Neurosci 49(1): 1-8.
  26. Ogawa, T., Hänggi, D., Wu, Y., Michiue, H., Tomizawa, K., Ono, S., Matsui, H., Date, I. and Steiger, H. J. (2011). Protein therapy using heme-oxygenase-1 fused to a polyarginine transduction domain attenuates cerebral vasospasm after experimental subarachnoid hemorrhage. J Cereb Blood Flow Metab 31(11): 2231-2242.
  27. Kaitsuka, T., Tomizawa, K. and Matsushita, M. (2011). Transformation of eEF1Bdelta into heat-shock response transcription factor by alternative splicing. EMBO Rep 12(7): 673-681.
  28. Li, A., Saito, M., Chuang, J. Z., Tseng, Y. Y., Dedesma, C., Tomizawa, K., Kaitsuka, T. and Sung, C. H. (2011). Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors. Nat Cell Biol 13(4): 402-411.
  29. Fujimura, A., Michiue, H., Nishiki, T., Ohmori, I., Wei, F. Y., Matsui, H. and Tomizawa, K. (2011). Expression of a constitutively active calcineurin encoded by an intron-retaining mRNA in follicular keratinocytes. PLoS One 6(3): e17685.
  30. Arragain, S., Handelman, S. K., Forouhar, F., Wei, F. Y., Tomizawa, K., Hunt, J. F., Douki, T., Fontecave, M., Mulliez, E. and Atta, M. (2010). Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA. J Biol Chem 285(37): 28425-28433.
  31. Matsushita, H., Tomizawa, K., Okimoto, N., Nishiki, T., Ohmori, I. and Matsui, H. (2010). Oxytocin mediates the antidepressant effects of mating behavior in male mice. Neurosci Res 68(2): 151-153.
  32. Feng, B., Tomizawa, K., Michiue, H., Han, X. J., Miyatake, S. and Matsui, H. (2010). Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo. Biomaterials 31(14): 4139-4145.
  33. Araki, D., Takayama, K., Inoue, M., Watanabe, T., Kumon, H., Futaki, S., Matsui, H. and Tomizawa, K. (2010). Cell-penetrating D-isomer peptides of p53 C-terminus: long-term inhibitory effect on the growth of bladder cancer. Urology 75(4): 813-819.
  34. Takayama, K., Nakase, I., Michiue, H., Takeuchi, T., Tomizawa, K., Matsui, H. and Futaki, S. (2009). Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas). J Control Release 138(2): 128-133.
  35. Nishiyama, Y., Yokoyama, T., Tomizawa, K., Okamura, K., Yamamoto, Y., Matsui, H., Oguma, K., Nagai, A. and Kumon, H. (2009). Effects of purified newly developed botulinum neurotoxin type A in rat prostate. Urology 74(2): 436-439.
  36. Feng, B., Tomizawa, K., Michiue, H., Miyatake, S., Han, X. J., Fujimura, A., Seno, M., Kirihata, M. and Matsui, H. (2009). Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His. Biomaterials 30(9): 1746-1755.
  37. Ohmori, I., Ouchida, M., Miki, T., Mimaki, N., Kiyonaka, S., Nishiki, T., Tomizawa, K., Mori, Y. and Matsui, H. (2008). A CACNB4 mutation shows that altered Ca(v)2.1 function may be a genetic modifier of severe myoclonic epilepsy in infancy. Neurobiol Dis 32(3): 349-354.
  38. Han, X. J., Lu, Y. F., Li, S. A., Kaitsuka, T., Sato, Y., Tomizawa, K., Nairn, A. C., Takei, K., Matsui, H. and Matsushita, M. (2008). CaM kinase I alpha-induced phosphorylation of Drp1 regulates mitochondrial morphology. J Cell Biol 182(3): 573-585.
  39. Han, X. J., Lu, Y. F., Li, S. A., Tomizawa, K., Takei, K., Matsushita, M. and Matsui, H. (2008). Involvement of calcineurin in glutamate-induced mitochondrial dynamics in neurons. Neurosci Res 60(1): 114-119.
  40. Taniike, N., Lu, Y. F., Tomizawa, K. and Matsui, H. (2008). Critical differences in magnitude and duration of N-methyl D-aspartate(NMDA) receptor activation between long-term potentiation (LTP) and long-term depression (LTD) induction. Acta Med Okayama 62(1): 21-28.
  41. Wu, Y., Liang, S., Oda, Y., Ohmori, I., Nishiki, T., Takei, K., Matsui, H. and Tomizawa, K. (2007). Truncations of amphiphysin I by calpain inhibit vesicle endocytosis during neural hyperexcitation. EMBO J 26(12): 2981-2990.
  42. Kitani, K., Oguma, S., Nishiki, T., Ohmori, I., Galons, H., Matsui, H., Meijer, L. and Tomizawa, K. (2007). A Cdk5 inhibitor enhances the induction of insulin secretion by exendin-4 both in vitro and in vivo. J Physiol Sci 57(4): 235-239.
  43. Yamada, H., Ohashi, E., Abe, T., Kusumi, N., Li, S. A., Yoshida, Y., Watanabe, M., Tomizawa, K., Kashiwakura, Y., Kumon, H., Matsui, H. and Takei, K. (2007). Amphiphysin 1 is important for actin polymerization during phagocytosis. Mol Biol Cell 18(11): 4669-4680.
  44. Tsutsui, Y., Tomizawa, K., Nagita, M., Michiue, H., Nishiki, T., Ohmori, I., Seno, M. and Matsui, H. (2007). Development of bionanocapsules targeting brain tumors. J Control Release 122(2): 159-164.
  45. Liang, S., Wei, F. Y., Wu, Y. M., Tanabe, K., Abe, T., Oda, Y., Yoshida, Y., Yamada, H., Matsui, H., Tomizawa, K. and Takei, K. (2007). Major Cdk5-dependent phosphorylation sites of amphiphysin 1 are implicated in the regulation of the membrane binding and endocytosis. J Neurochem 102(5): 1466-1476.
  46. Ogawa, T., Ono, S., Ichikawa, T., Arimitsu, S., Onoda, K., Tokunaga, K., Sugiu, K., Tomizawa, K., Matsui, H. and Date, I. (2007). Novel protein transduction method by using 11R: an effective new drug delivery system for the treatment of cerebrovascular diseases. Stroke 38(4): 1354-1361.
  47. Wu, Y., Tada, M., Takahata, K., Tomizawa, K. and Matsui, H. (2007). Inhibitory effect of polyunsaturated fatty acids on apoptosis induced by etoposide, okadaic acid and AraC in Neuro2a cells. Acta Med Okayama 61(3): 147-152.
  48. Fujisawa, T., Moriwaki, A., Matsushita, M., Tomizawa, K. and Matsui, H. (2007). Colocalization of oxytocin and phosphorylated form of elongation factor 2 in the rat hypothalamus. Acta Med Okayama 61(3): 161-166.
  49. Kuriyama, M., Matsushita, M., Tateishi, A., Moriwaki, A., Tomizawa, K., Ishino, K., Sano, S. and Matsui, H. (2006). A cell-permeable NFAT inhibitor peptide prevents pressure-overload cardiac hypertrophy. Chem Biol Drug Des 67(3): 238-243.
  50. Inoue, M., Tomizawa, K., Matsushita, M., Lu, Y. F., Yokoyama, T., Yanai, H., Takashima, A., Kumon, H. and Matsui, H. (2006). p53 protein transduction therapy: successful targeting and inhibition of the growth of the bladder cancer cells. Eur Urol 49(1): 161-168.
  51. Wei, F. Y., Nagashima, K., Ohshima, T., Saheki, Y., Lu, Y. F., Matsushita, M., Yamada, Y., Mikoshiba, K., Seino, Y., Matsui, H. and Tomizawa, K. (2005). Cdk5-dependent regulation of glucose-stimulated insulin secretion. Nat Med 11(10): 1104-1108.
  52. Liu, F., Grundke-Iqbal, I., Iqbal, K., Oda, Y., Tomizawa, K. and Gong, C. X. (2005). Truncation and activation of calcineurin A by calpain I in Alzheimer disease brain. J Biol Chem 280(45): 37755-37762.
  53. Ohshima, T., Ogura, H., Tomizawa, K., Hayashi, K., Suzuki, H., Saito, T., Kamei, H., Nishi, A., Bibb, J. A., Hisanaga, S., Matsui, H. and Mikoshiba, K. (2005). Impairment of hippocampal long-term depression and defective spatial learning and memory in p35 mice. J Neurochem 94(4): 917-925.
  54. Wu, H. Y., Yuen, E. Y., Lu, Y. F., Matsushita, M., Matsui, H., Yan, Z. and Tomizawa, K. (2005). Regulation of N-methyl-D-aspartate receptors by calpain in cortical neurons. J Biol Chem 280(22): 21588-21593.
  55. Matsushita, M., Kozak, J. A., Shimizu, Y., McLachlin, D. T., Yamaguchi, H., Wei, F. Y., Tomizawa, K., Matsui, H., Chait, B. T., Cahalan, M. D. and Nairn, A. C. (2005). Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1. J Biol Chem 280(21): 20793-20803.
  56. Wei, F. Y., Tomizawa, K., Ohshima, T., Asada, A., Saito, T., Nguyen, C., Bibb, J. A., Ishiguro, K., Kulkarni, A. B., Pant, H. C., Mikoshiba, K., Matsui, H. and Hisanaga, S. (2005). Control of cyclin-dependent kinase 5 (Cdk5) activity by glutamatergic regulation of p35 stability. J Neurochem 93(2): 502-512.
  57. Saheki, Y., Li, S. T., Matsushita, M., Wu, Y. M., Cai, W. H., Wei, F. Y., Lu, Y. F., Moriwaki, A., Tomizawa, K. and Matsui, H. (2005). A new approach to inhibiting astrocytic IP3-induced intracellular calcium increase in an astrocyte-neuron co-culture system. Brain Res 1055(1-2): 196-201.
  58. Michiue, H., Tomizawa, K., Matsushita, M., Tamiya, T., Lu, Y. F., Ichikawa, T., Date, I. and Matsui, H. (2005). Ubiquitination-resistant p53 protein transduction therapy facilitates anti-cancer effect on the growth of human malignant glioma cells. FEBS Lett 579(18): 3965-3969.
  59. Kumashiro, S., Lu, Y. F., Tomizawa, K., Matsushita, M., Wei, F. Y. and Matsui, H. (2005). Regulation of synaptic vesicle recycling by calcineurin in different vesicle pools. Neurosci Res 51(4): 435-443.
  60. Michiue, H., Tomizawa, K., Wei, F. Y., Matsushita, M., Lu, Y. F., Ichikawa, T., Tamiya, T., Date, I. and Matsui, H. (2005). The NH2 terminus of influenza virus hemagglutinin-2 subunit peptides enhances the antitumor potency of polyarginine-mediated p53 protein transduction. J Biol Chem 280(9): 8285-8289.
  61. Arataki, S., Tomizawa, K., Moriwaki, A., Nishida, K., Matsushita, M., Ozaki, T., Kunisada, T., Yoshida, A., Inoue, H. and Matsui, H. (2005). Calpain inhibitors prevent neuronal cell death and ameliorate motor disturbances after compression-induced spinal cord injury in rats. J Neurotrauma 22(3): 398-406.
  62. Takata, H., Tomizawa, K., Matsushita, M. and Matsui, H. (2004). 2-methoxyestradiol enhances p53 protein transduction therapy-associated inhibition of the proliferation of oral cancer cells through the suppression of NFkappaB activity. Acta Med Okayama 58(4): 181-187.
  63. Ishii, M., Tomizawa, K., Matsushita, M. and Matsui, H. (2004). Exposure of mouse to high gravitation forces induces long-term potentiation in the hippocampus. Acta Med Okayama 58(3): 143-149.
  64. Noguchi, H., Matsushita, M., Okitsu, T., Moriwaki, A., Tomizawa, K., Kang, S., Li, S. T., Kobayashi, N., Matsumoto, S., Tanaka, K., Tanaka, N. and Matsui, H. (2004). A new cell-permeable peptide allows successful allogeneic islet transplantation in mice. Nat Med 10(3): 305-309.
  65. Li, S. T., Matsushita, M., Moriwaki, A., Saheki, Y., Lu, Y. F., Tomizawa, K., Wu, H. Y., Terada, H. and Matsui, H. (2004). HIV-1 Tat inhibits long-term potentiation and attenuates spatial learning [corrected]. Ann Neurol 55(3): 362-371.
  66. Wu, H. Y., Tomizawa, K., Oda, Y., Wei, F. Y., Lu, Y. F., Matsushita, M., Li, S. T., Moriwaki, A. and Matsui, H. (2004). Critical role of calpain-mediated cleavage of calcineurin in excitotoxic neurodegeneration. J Biol Chem 279(6): 4929-4940.
  67. Matsushita, M., Noguchi, H., Lu, Y. F., Tomizawa, K., Michiue, H., Li, S. T., Hirose, K., Bonner-Weir, S. and Matsui, H. (2004). Photo-acceleration of protein release from endosome in the protein transduction system. FEBS Lett 572(1-3): 221-226.
  68. Tomizawa, K., Iga, N., Lu, Y. F., Moriwaki, A., Matsushita, M., Li, S. T., Miyamoto, O., Itano, T. and Matsui, H. (2003). Oxytocin improves long-lasting spatial memory during motherhood through MAP kinase cascade. Nat Neurosci 6(4): 384-390. *On caver page
  69. Tomizawa, K., Sunada, S., Lu, Y. F., Oda, Y., Kinuta, M., Ohshima, T., Saito, T., Wei, F. Y., Matsushita, M., Li, S. T., Tsutsui, K., Hisanaga, S., Mikoshiba, K., Takei, K. and Matsui, H. (2003). Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles. J Cell Biol 163(4): 813-824.
  70. Terada, H., Matsushita, M., Lu, Y. F., Shirai, T., Li, S. T., Tomizawa, K., Moriwaki, A., Nishio, S., Date, I., Ohmoto, T. and Matsui, H. (2003). Inhibition of excitatory neuronal cell death by cell-permeable calcineurin autoinhibitory peptide. J Neurochem 87(5): 1145-1151.
  71. Nozaki, K., Tomizawa, K., Yokoyama, T., Kumon, H. and Matsui, H. (2003). Calcineurin mediates bladder smooth muscle hypertrophy after bladder outlet obstruction. J Urol 170(5): 2077-2081.
  72. Wu, H. Y., Tomizawa, K., Matsushita, M., Lu, Y. F., Li, S. T. and Matsui, H. (2003). Poly-arginine-fused calpastatin peptide, a living cell membrane-permeable and specific inhibitor for calpain. Neurosci Res 47(1): 131-135.
  73. Yamashita, S., Miyamoto, O., Janjua, N. A., Tomizawa, K., Matsui, H., Nakamura, T., Nagao, S. and Itano, T. (2003). Role of the hippocampal CA2 region following postischemic hypothermia in gerbil. Brain Res Mol Brain Res 111(1-2): 8-16.
  74. Li, S. T., Kato, K., Tomizawa, K., Matsushita, M., Moriwaki, A., Matsui, H. and Mikoshiba, K. (2002). Calcineurin plays different roles in group II metabotropic glutamate receptor- and NMDA receptor-dependent long-term depression. J Neurosci 22(12): 5034-5041.
  75. Takenobu, T., Tomizawa, K., Matsushita, M., Li, S. T., Moriwaki, A., Lu, Y. F. and Matsui, H. (2002). Development of p53 protein transduction therapy using membrane-permeable peptides and the application to oral cancer cells. Mol Cancer Ther 1(12): 1043-1049.
  76. Matsushita, M., Tomizawa, K., Moriwaki, A., Li, S. T., Terada, H. and Matsui, H. (2001). A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation. J Neurosci 21(16): 6000-6007.
  77. Tomizawa, K., Cai, X. H., Moriwaki, A., Matsushita, M. and Matsui, H. (2000). Involvement of cyclin-dependent kinase 5/p35(nck5a) in the synaptic reorganization of rat hippocampus during kindling progression. Jpn J Physiol 50(5): 525-532.
  78. Feng, J., Yan, Z., Ferreira, A., Tomizawa, K., Liauw, J. A., Zhuo, M., Allen, P. B., Ouimet, C. C. and Greengard, P. (2000). Spinophilin regulates the formation and function of dendritic spines. Proc Natl Acad Sci U S A 97(16): 9287-9292.
  79. Yan, Z., Hsieh-Wilson, L., Feng, J., Tomizawa, K., Allen, P. B., Fienberg, A. A., Nairn, A. C. and Greengard, P. (1999). Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin. Nat Neurosci 2(1): 13-17.
  80. Lu, Y. F., Kojima, N., Tomizawa, K., Moriwaki, A., Matsushita, M., Obata, K. and Matsui, H. (1999). Enhanced synaptic transmission and reduced threshold for LTP induction in fyn-transgenic mice. Eur J Neurosci 11(1): 75-82.
Protocols by Kazuhito Tomizawa
  1. Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR