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Sérgio R. Filipe

Sérgio R. Filipe


Ph.D. in Biology, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal, 2001

Current position

Assistant Professor, Department of Life Sciences, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal (2015-present)
Head of the research group of Bacterial Cell Surfaces and Pathogenesis, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Portugal (2004-present)


*Co-first authors

  1. Monteiro, J. M., Fernandes, P. B., Vaz, F., Pereira, A. R., Tavares, A. C., Ferreira, M. T., Pereira, P. M., Veiga, H., Kuru, E., VanNieuwenhze, M. S., Brun, Y. V., Filipe, S. R. and Pinho, M. G. (2015). Cell shape dynamics during the staphylococcal cell cycle. Nat Commun 6: 8055.
  2. Greene, N. G., Narciso, A. R., Filipe, S. R. and Camilli, A. (2015). Peptidoglycan Branched Stem Peptides Contribute to Streptococcus pneumoniae Virulence by Inhibiting Pneumolysin Release. PLoS Pathog 11(6): e1004996.
  3. Carvalho, F., Atilano, M. L., Pombinho, R., Covas, G., Gallo, R. L., Filipe, S. R., Sousa, S. and Cabanes, D. (2015). L-Rhamnosylation of Listeria monocytogenes Wall Teichoic Acids Promotes Resistance to Antimicrobial Peptides by Delaying Interaction with the Membrane. PLoS Pathog 11(5): e1004919.
  4. Reed, P., Atilano, M. L., Alves, R., Hoiczyk, E., Sher, X., Reichmann, N. T., Pereira, P. M., Roemer, T., Filipe, S. R., Pereira-Leal, J. B., Ligoxygakis, P. and Pinho, M. G. (2015). Staphylococcus aureus Survives with a Minimal Peptidoglycan Synthesis Machine but Sacrifices Virulence and Antibiotic Resistance. PLoS Pathog 11(5): e1004891.
  5. Catalao, M. J., Figueiredo, J., Henriques, M. X., Gomes, J. P. and Filipe, S. R. (2014). Optimization of fluorescent tools for cell biology studies in Gram-positive bacteria. PLoS One 9(12): e113796.
  6. Atilano, M. L., Pereira, P. M., Vaz, F., Catalao, M. J., Reed, P., Grilo, I. R., Sobral, R. G., Ligoxygakis, P., Pinho, M. G. and Filipe, S. R. (2014). Bacterial autolysins trim cell surface peptidoglycan to prevent detection by the Drosophila innate immune system. Elife 3: e02277.
  7. Rueff, A. S., Chastanet, A., Dominguez-Escobar, J., Yao, Z., Yates, J., Prejean, M. V., Delumeau, O., Noirot, P., Wedlich-Soldner, R., Filipe, S. R. and Carballido-Lopez, R. (2014). An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis. Mol Microbiol 91(2): 348-362.
  8. Thorsing, M., Klitgaard, J. K., Atilano, M. L., Skov, M. N., Kolmos, H. J., Filipe, S. R. and Kallipolitis, B. H. (2013). Thioridazine induces major changes in global gene expression and cell wall composition in methicillin-resistant Staphylococcus aureus USA300. PLoS One 8(5): e64518.
  9. Henriques, M. X.*, Catalao, M. J.*, Figueiredo, J., Gomes, J. P. and Filipe, S. R. (2013). Construction of improved tools for protein localization studies in Streptococcus pneumoniae. PLoS One 8(1): e55049.
  10. Atilano, M. L.^, Yates, J.^, Glittenberg, M.^, Filipe, S. R.* and Ligoxygakis, P.* (2011). Wall teichoic acids of Staphylococcus aureus limit recognition by the drosophila peptidoglycan recognition protein-SA to promote pathogenicity. PLoS Pathog 7(12): e1002421.
  11. Henriques, M. X., Rodrigues, T., Carido, M., Ferreira, L. and Filipe, S. R. (2011). Synthesis of capsular polysaccharide at the division septum of Streptococcus pneumoniae is dependent on a bacterial tyrosine kinase. Mol Microbiol 82(2): 515-534.
  12. Atilano, M. L., Pereira, P. M., Yates, J., Reed, P., Veiga, H., Pinho, M. G. and Filipe, S. R.* (2010). Teichoic acids are temporal and spatial regulators of peptidoglycan cross-linking in Staphylococcus aureus. Proc Natl Acad Sci U S A 107(44): 18991-18996.
  13. Memmi, G., Filipe, S. R., Pinho, M. G., Fu, Z. and Cheung, A. (2008). Staphylococcus aureus PBP4 is essential for beta-lactam resistance in community-acquired methicillin-resistant strains. Antimicrob Agents Chemother 52(11): 3955-3966.
  14. Wang, L., Gilbert, R. J., Atilano, M. L., Filipe, S. R., Gay, N. J. and Ligoxygakis, P. (2008). Peptidoglycan recognition protein-SD provides versatility of receptor formation in Drosophila immunity. Proc Natl Acad Sci U S A 105(33): 11881-11886.
  15. Pereira, P. M., Filipe, S. R., Tomasz, A. and Pinho, M. G. (2007). Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 51(10): 3627-3633.
  16. Wang, L., Weber, A. N., Atilano, M. L., Filipe, S. R., Gay, N. J. and Ligoxygakis, P. (2006). Sensing of Gram-positive bacteria in Drosophila: GNBP1 is needed to process and present peptidoglycan to PGRP-SA. EMBO J 25(20): 5005-5014.
  17. Payne, B. T., van Knippenberg, I. C., Bell, H., Filipe, S. R., Sherratt, D. J. and McGlynn, P. (2006). Replication fork blockage by transcription factor-DNA complexes in Escherichia coli. Nucleic Acids Res 34(18): 5194-5202.
  18. Possoz, C.*, Filipe, S. R.*, Grainge, I. and Sherratt, D. J. (2006). Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo. EMBO J 25(11): 2596-2604.
  19. Filipe, S. R., Tomasz, A. and Ligoxygakis, P. (2005). Requirements of peptidoglycan structure that allow detection by the Drosophila Toll pathway. EMBO Rep 6(4): 327-333.
  20. Gardete, S., Ludovice, A. M., Sobral, R. G., Filipe, S. R., de Lencastre, H. and Tomasz, A. (2004). Role of murE in the Expression of beta-lactam antibiotic resistance in Staphylococcus aureus. J Bacteriol 186(6): 1705-1713.
  21. Sherratt, D. J., Soballe, B., Barre, F. X., Filipe, S. R., Massey, T., Lau, I., and J. Yates. (2004). Recombination and chromosome segregation. Phil Trans R Soc Lond B 359: 61-69.
  22. Fiser, A., Filipe, S. R. and Tomasz, A. (2003). Cell wall branches, penicillin resistance and the secrets of the MurM protein. Trends Microbiol 11(12): 547-553.
  23. Lau, I. F., Filipe, S. R., Soballe, B., Okstad, O. A., Barre, F. X. and Sherratt, D. J. (2003). Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol Microbiol 49(3): 731-743.
  24. Filipe, S. R., Severina, E. and Tomasz, A. (2002). The murMN operon: a functional link between antibiotic resistance and antibiotic tolerance in Streptococcus pneumoniae. Proc Natl Acad Sci U S A 99(3): 1550-1555.
  25. Filipe, S. R., Severina, E. and Tomasz, A. (2001). The role of murMN operon in penicillin resistance and antibiotic tolerance of Streptococcus pneumoniae. Microb Drug Resist 7(4): 303-316.
  26. Pinho, M. G., Filipe, S. R., de Lencastre, H. and Tomasz, A. (2001). Complementation of the essential peptidoglycan transpeptidase function of penicillin-binding protein 2 (PBP2) by the drug resistance protein PBP2A in Staphylococcus aureus. J Bacteriol 183(22): 6525-6531.
  27. Filipe, S. R., Severina, E. and Tomasz, A. (2001). Functional analysis of Streptococcus pneumoniae MurM reveals the region responsible for its specificity in the synthesis of branched cell wall peptides. J Biol Chem 276(43): 39618-39628.
  28. Filipe, S. R., Severina, E. and Tomasz, A. (2000). Distribution of the mosaic structured murM genes among natural populations of Streptococcus pneumoniae. J Bacteriol 182(23): 6798-6805.
  29. Filipe, S. R., Pinho, M. G. and Tomasz, A. (2000). Characterization of the murMN operon involved in the synthesis of branched peptidoglycan peptides in Streptococcus pneumoniae. J Biol Chem 275(36): 27768-27774.
  30. Filipe, S. R. and Tomasz, A. (2000). Inhibition of the expression of penicillin resistance in Streptococcus pneumoniae by inactivation of cell wall muropeptide branching genes. Proc Natl Acad Sci U S A 97(9): 4891-4896.
  31. De Lencastre, H., Wu, S. W., Pinho, M. G., Ludovice, A. M., Filipe, S., Gardete, S., Sobral, R. and Gill, S. (1999). Antibiotic resistance as a stress response: Complete sequencing of a large number ofchromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance tomethicillin. Microb Drug Resist 5:163-175.
Protocols by Sérgio R. Filipe
  1. Preparation and Analysis of Crude Autolytic Enzyme Extracts from Staphylococcus aureus