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Carl J. Douglas

Education

Ph.D. in Botany, University of Washington, 1983

Current position

Professor, Department of Botany, University of British Columbia (1987-present)

Publications (2009 to present)

  1. Pinard, D., Mizrachi, E., Hefer, C. A., Kersting, A. R., Joubert, F., Douglas, C. J., Mansfield, S. D. and Myburg, A. A. (2015). Comparative analysis of plant carbohydrate active enZymes and their role in xylogenesis. BMC Genomics 16: 402.
  2. Geraldes, A., Hefer, C. A., Capron, A., Kolosova, N., Martinez-Nunez, F., Soolanayakanahally, R. Y., Stanton, B., Guy, R. D., Mansfield, S. D., Douglas, C. J. and Cronk, Q. C. (2015). Recent Y chromosome divergence despite ancient origin of dioecy in poplars (Populus). Mol Ecol 24(13): 3243-3256. (cover and commentary feature)
  3. Liu, Y. and Douglas, C. J. (2015). A role for OVATE FAMILY PROTEIN1 (OFP1) and OFP4 in a BLH6-KNAT7 multi-protein complex regulating secondary cell wall formation in Arabidopsis thaliana. Plant Signal Behav 10(7): e1033126.
  4. Hefer, C. A., Mizrachi, E., Myburg, A. A., Douglas, C. J. and Mansfield, S. D. (2015). Comparative interrogation of the developing xylem transcriptomes of two wood-forming species: Populus trichocarpa and Eucalyptus grandis. New Phytol 206(4): 1391-1405.
  5. Muchero, W., Guo, J., DiFazio, S. P., Chen, J. G., Ranjan, P., Slavov, G. T., Gunter, L. E., Jawdy, S., Bryan, A. C., Sykes, R., Ziebell, A., Klapste, J., Porth, I., Skyba, O., Unda, F., El-Kassaby, Y. A., Douglas, C. J., Mansfield, S. D., Martin, J., Schackwitz, W., Evans, L. M., Czarnecki, O. and Tuskan, G. A. (2015). High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus. BMC Genomics 16: 24.
  6. Liu, Y., You, S., Taylor-Teeples, M., Li, W. L., Schuetz, M., Brady, S. M. and Douglas, C. J. (2014). BEL1-LIKE HOMEODOMAIN6 and KNOTTED ARABIDOPSIS THALIANA7 interact and regulate secondary cell wall formation via repression of REVOLUTA. Plant Cell 26(12): 4843-4861.
  7. Quilichini, T. D., Samuels, A. L. and Douglas, C. J. (2014). ABCG26-mediated polyketide trafficking and hydroxycinnamoyl spermidines contribute to pollen wall exine formation in Arabidopsis. Plant Cell 26(11): 4483-4498.
  8. Wang, S., Li, E., Porth, I., Chen, J. G., Mansfield, S. D. and Douglas, C. J. (2014). Regulation of secondary cell wall biosynthesis by poplar R2R3 MYB transcription factor PtrMYB152 in Arabidopsis. Sci Rep 4: 5054.
  9. Huang, D. I., Hefer, C. A., Kolosova, N., Douglas, C. J. and Cronk, Q. C. (2014). Whole plastome sequencing reveals deep plastid divergence and cytonuclear discordance between closely related balsam poplars, Populus balsamifera and P. trichocarpa (Salicaceae). New Phytol 204(3): 693-703.
  10. Geraldes, A., Farzaneh, N., Grassa, C. J., McKown, A. D., Guy, R. D., Mansfield, S. D., Douglas, C. J. and Cronk, Q. C. (2014). Landscape genomics of Populus trichocarpa: the role of hybridization, limited gene flow, and natural selection in shaping patterns of population structure. Evolution 68(11): 3260-3280.
  11. Quilichini, T. D., Grienenberger, E. and Douglas, C. J. (2015). The biosynthesis, composition and assembly of the outer pollen wall: A tough case to crack. Phytochemistry 113: 170-182.
  12. Quilichini, T. D., Douglas, C. J. and Samuels, A. L. (2014). New views of tapetum ultrastructure and pollen exine development in Arabidopsis thaliana. Ann Bot 114(6): 1189-1201.
  13. McKown, A. D., Klapste, J., Guy, R. D., Geraldes, A., Porth, I., Hannemann, J., Friedmann, M., Muchero, W., Tuskan, G. A., Ehlting, J., Cronk, Q. C., El-Kassaby, Y. A., Mansfield, S. D. and Douglas, C. J. (2014). Genome-wide association implicates numerous genes underlying ecological trait variation in natural populations of Populus trichocarpa. New Phytol 203(2): 535-553.
  14. Porth, I., Klapste, J., McKown, A. D., La Mantia, J., Hamelin, R. C., Skyba, O., Unda, F., Friedmann, M. C., Cronk, Q. C., Ehlting, J., Guy, R. D., Mansfield, S. D., El-Kassaby, Y. A. and Douglas, C. J. (2014). Extensive functional pleiotropy of REVOLUTA substantiated through forward genetics. Plant Physiol 164(2): 548-554.
  15. Grienenberger, E. and Douglas, C. J. (2014). Arabidopsis VASCULAR-RELATED UNKNOWN PROTEIN1 regulates xylem development and growth by a conserved mechanism that modulates hormone signaling. Plant Physiol 164(4): 1991-2010.
  16. McKown, A. D., Guy, R. D., Klapste, J., Geraldes, A., Friedmann, M., Cronk, Q. C., El-Kassaby, Y. A., Mansfield, S. D. and Douglas, C. J. (2014). Geographical and environmental gradients shape phenotypic trait variation and genetic structure in Populus trichocarpa. New Phytol 201(4): 1263-1276.
  17. La Mantia, J., Klapste, J., El-Kassaby, Y. A., Azam, S., Guy, R. D., Douglas, C. J., Mansfield, S. D. and Hamelin, R. (2013). Association analysis identifies Melampsora xcolumbiana poplar leaf rust resistance SNPs. PLoS One 8(11): e78423.
  18. Bao, H., Li, E., Mansfield, S. D., Cronk, Q. C., El-Kassaby, Y. A. and Douglas, C. J. (2013). The developing xylem transcriptome and genome-wide analysis of alternative splicing in Populus trichocarpa (black cottonwood) populations. BMC Genomics 14: 359.
  19. Porth, I., Klapste, J., Skyba, O., Hannemann, J., McKown, A. D., Guy, R. D., DiFazio, S. P., Muchero, W., Ranjan, P., Tuskan, G. A., Friedmann, M. C., Ehlting, J., Cronk, Q. C., El-Kassaby, Y. A., Douglas, C. J. and Mansfield, S. D. (2013). Genome-wide association mapping for wood characteristics in Populus identifies an array of candidate single nucleotide polymorphisms. New Phytol 200(3): 710-726.
  20. Porth, I., Klapste, J., Skyba, O., Friedmann, M. C., Hannemann, J., Ehlting, J., El-Kassaby, Y. A., Mansfield, S. D. and Douglas, C. J. (2013). Network analysis reveals the relationship among wood properties, gene expression levels and genotypes of natural Populus trichocarpa accessions. New Phytol 200(3): 727-742.
  21. Bhargava, A., Ahad, A., Wang, S., Mansfield, S. D., Haughn, G. W., Douglas, C. J. and Ellis, B. E. (2013). The interacting MYB75 and KNAT7 transcription factors modulate secondary cell wall deposition both in stems and seed coat in Arabidopsis. Planta 237(5): 1199-1211.
  22. You, M., Yue, Z., He, W., Yang, X., Yang, G., Xie, M., Zhan, D., Baxter, S. W., Vasseur, L., Gurr, G. M., Douglas, C. J., Bai, J., Wang, P., Cui, K., Huang, S., Li, X., Zhou, Q., Wu, Z., Chen, Q., Liu, C., Wang, B., Li, X., Xu, X., Lu, C., Hu, M., Davey, J. W., Smith, S. M., Chen, M., Xia, X., Tang, W., Ke, F., Zheng, D., Hu, Y., Song, F., You, Y., Ma, X., Peng, L., Zheng, Y., Liang, Y., Chen, Y., Yu, L., Zhang, Y., Liu, Y., Li, G., Fang, L., Li, J., Zhou, X., Luo, Y., Gou, C., Wang, J., Wang, J., Yang, H. and Wang, J. (2013). A heterozygous moth genome provides insights into herbivory and detoxification. Nat Genet 45(2): 220-225.
  23. Geraldes, A., Difazio, S. P., Slavov, G. T., Ranjan, P., Muchero, W., Hannemann, J., Gunter, L. E., Wymore, A. M., Grassa, C. J., Farzaneh, N., Porth, I., McKown, A. D., Skyba, O., Li, E., Fujita, M., Klapste, J., Martin, J., Schackwitz, W., Pennacchio, C., Rokhsar, D., Friedmann, M. C., Wasteneys, G. O., Guy, R. D., El-Kassaby, Y. A., Mansfield, S. D., Cronk, Q. C., Ehlting, J., Douglas, C. J. and Tuskan, G. A. (2013). A 34K SNP genotyping array for Populus trichocarpa: design, application to the study of natural populations and transferability to other Populus species. Mol Ecol Resour 13(2): 306-323.
  24. Porth, I., Klapste, J., Skyba, O., Lai, B. S., Geraldes, A., Muchero, W., Tuskan, G. A., Douglas, C. J., El-Kassaby, Y. A. and Mansfield, S. D. (2013). Populus trichocarpa cell wall chemistry and ultrastructure trait variation, genetic control and genetic correlations. New Phytol 197(3): 777-790.
  25. Slavov, G. T., DiFazio, S. P., Martin, J., Schackwitz, W., Muchero, W., Rodgers-Melnick, E., Lipphardt, M. F., Pennacchio, C. P., Hellsten, U., Pennacchio, L. A., Gunter, L. E., Ranjan, P., Vining, K., Pomraning, K. R., Wilhelm, L. J., Pellegrini, M., Mockler, T. C., Freitag, M., Geraldes, A., El-Kassaby, Y. A., Mansfield, S. D., Cronk, Q. C., Douglas, C. J., Strauss, S. H., Rokhsar, D. and Tuskan, G. A. (2012). Genome resequencing reveals multiscale geographic structure and extensive linkage disequilibrium in the forest tree Populus trichocarpa. New Phytol 196(3): 713-725.
  26. Li, E., Bhargava, A., Qiang, W., Friedmann, M. C., Forneris, N., Savidge, R. A., Johnson, L. A., Mansfield, S. D., Ellis, B. E. and Douglas, C. J. (2012). The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus. New Phytologist 194(1): 102-115.
  27. Li, E., Wang, S., Liu, Y., Chen, J. G. and Douglas, C. J. (2011). OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana. Plant J 67(2): 328-341.
  28. Geraldes, A., Pang, J., Thiessen, N., Cezard, T., Moore, R., Zhao, Y., Tam, A., Wang, S., Friedmann, M., Birol, I., Jones, S. J., Cronk, Q. C. and Douglas, C. J. (2011). SNP discovery in black cottonwood (Populus trichocarpa) by population transcriptome resequencing. Mol Ecol Resour 11 Suppl 1: 81-92.
  29. Kim, S. S., Grienenberger, E., Lallemand, B., Colpitts, C. C., Kim, S. Y., Souza Cde, A., Geoffroy, P., Heintz, D., Krahn, D., Kaiser, M., Kombrink, E., Heitz, T., Suh, D. Y., Legrand, M. and Douglas, C. J. (2010). LAP6/POLYKETIDE SYNTHASE A and LAP5/POLYKETIDE SYNTHASE B encode hydroxyalkyl alpha-pyrone synthases required for pollen development and sporopollenin biosynthesis in Arabidopsis thaliana. Plant Cell 22(12): 4045-4066.
  30. Grienenberger, E., Kim, S. S., Lallemand, B., Geoffroy, P., Heintz, D., Souza Cde, A., Heitz, T., Douglas, C. J. and Legrand, M. (2010). Analysis of TETRAKETIDE alpha-PYRONE REDUCTASE function in Arabidopsis thaliana reveals a previously unknown, but conserved, biochemical pathway in sporopollenin monomer biosynthesis. Plant Cell 22(12): 4067-4083.
  31. Kim, J. E., Cheng, K. M., Craft, N. E., Hamberger, B. and Douglas, C. J. (2010). Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a beta-carotene ketolase provides insight into in vivo functions. Phytochemistry 71(2-3): 168-178.
  32. Polle, A. and Douglas, C. (2010). The molecular physiology of poplars: paving the way for knowledge‐based biomass production. Plant Biology 12(2): 239-241.
  33. Quilichini, T. D., Friedmann, M. C., Samuels, A. L. and Douglas, C. J. (2010). ATP-binding cassette transporter G26 is required for male fertility and pollen exine formation in Arabidopsis. Plant Physiol 154(2): 678-690.
  34. Bhargava, A., Mansfield, S. D., Hall, H. C., Douglas, C. J. and Ellis, B. E. (2010). MYB75 functions in regulation of secondary cell wall formation in the Arabidopsis inflorescence stem. Plant Physiol 154(3): 1428-1438.
  35. de Azevedo Souza, C., Kim, S. S., Koch, S., Kienow, L., Schneider, K., McKim, S. M., Haughn, G. W., Kombrink, E. and Douglas, C. J. (2009). A novel fatty Acyl-CoA Synthetase is required for pollen development and sporopollenin biosynthesis in Arabidopsis. Plant Cell 21(2): 507-525.
Protocols by Carl J. Douglas
  1. Analysis of Developing Pollen Grains within Intact Arabidopsis thaliana Anthers by Olympus Two-Photon Laser Scanning Microscopy