Education

Ph.D. in Life Science, Graduate School of Frontier Sciences, The University of Tokyo, 2009

Current position

Project Assistant Professor, Department of Integrated Biosciences, Graduate School of Frontier Science, The University of Tokyo, Kashiwa, Chiba, Japan

Publications

*Equal contribution

1. Akita, K., Higaki, T., Kutsuna, N. and Hasezawa, S. (2015). Quantitative analysis of microtubule orientation in interdigitated leaf pavement cells. Plant Signal Behav 10(5): e1024396.
   
2. Hirakawa, Y., Nomura, T., Hasezawa, S. and Higaki, T. (2015). Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora. J Integr Plant Biol 57(1): 127-135.
   
3. Higaki, T.*, Kutsuna, N., Akita, K., Sato, M., Sawaki, F., Kobayashi, M., Nagata, N., Toyooka, K. and Hasezawa, S. (2015). Semi-automatic organelle detection on transmission electron microscopic images. Sci Rep 5: 7794.
   
4. Higaki, T.*, Kato, A., Myouga, F., Kutsuna, N., Hasezawa, S. and Nagata, N. (2014). Automatic classification of chloroplast ultrastructure mutants with transmission electron microscopy. Bioimages 22: 1-7.
   
5. Higaki, T.*, Kutsuna, N. and Hasezawa, S. (2014). CARTA-based semi-automatic detection of stomatal regions on an Arabidopsis cotyledon surface. Plant Morphology 26:9-12.
   
6. Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. and Matsuoka, K. (2014). Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log to stationary transition of growth phase in tobacco BY-2 cells. Plant Cell Physiol 55(9): 1544-1555.
   
7. Hasegawa, J., Higaki, T., Hamamura, Y., Kurihara, D., Kutsuna, N., Higashiyama, T., Hasezawa, S. and Matsunaga, S. (2014). Increase in invaginated vacuolar membrane structure caused by plant cell expansion by genotoxic stress induced by DNA double-strand breaks. Cytologia 79:467-474.
   
8. Higaki, T.*, Hashimoto-Sugimoto, M., Akita, K., Iba, K. and Hasezawa, S. (2014). Dynamics and environmental responses of PATROL1 in Arabidopsis subsidiary cells. Plant Cell Physiol 55(4): 773-780.
   
9. Higaki, T.*, Higaki, S., Hirota, M. and Hasezawa, S. (2014). Radiocesium distribution in bamboo shoots after the Fukushima nuclear accident. PLoS One 9(5): e97659.
   
10. Hirota, M., Higaki, S., Higaki, T. and Hasezawa, S. (2013). Investigation of contamination in spinach collected immediately following the Fukushima Daiichi nuclear disaster. Rad Safe Manag 12:43-47.
    
11. Akita, K., Hasezawa, S. and Higaki, T. (2013). Breaking of plant stomatal one-cell-spacing rule by sugar solution immersion. PLoS One 8(9): e72456.
    
12. Kojo, K. H., Higaki, T., Kutsuna, N., Yoshida, Y., Yasuhara, H. and Hasezawa, S. (2013). Roles of cortical actin microfilament patterning in division plane orientation in plants. Plant Cell Physiol 54(9): 1491-1503.
    
13. Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi, M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K. (2013). A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses. Nat Commun 4: 2215.
    
14. Higaki, T.*, Kutsuna, N. and Hasezawa, S. (2013). LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells. BMC Plant Biol 13: 81.
    
15. Era, A., Kutsuna, N., Higaki, T., Hasezawa, S., Nakano, A. and Ueda, T. (2013). Microtubule stability affects the unique motility of F-actin in Marchantia polymorpha. J Plant Res 126(1): 113-119.
    
16. Kutsuna, N.*, Higaki, T.*, Matsunaga, S.*, Otsuki, T., Yamaguchi, M., Fujii, H. and Hasezawa, S. (2012). Active learning framework with iterative clustering for bioimage classification. Nat Commun 3: 1032.
    
17. Higaki, T.*, Kutsuna, N. and Hasezawa, S. (2012). Probabilistic mapping and image clustering for quantitative assessment of fluorescent protein localizations in Arabidopsis guard cells. Protocol Exchange.
    
18. Higaki, T.*, Kutsuna, N., Hosokawa, Y., Akita, K., Ebine, K., Ueda, T., Kondo, N. and Hasezawa, S. (2012). Statistical organelle dissection of Arabidopsis guard cells using image database LIPS. Sci Rep 2: 405.
    
19. Higaki, T.*, Higaki, S., Hirota, M., Akita, K. and Hasezawa, S. (2012). Radionuclide analysis on bamboos following the Fukushima nuclear accident. PLoS One 7(4): e34766.
    
20. Higaki, T.*, Kurusu, T., Hasezawa, S. and Kuchitsu, K. (2011). Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants. J Plant Res 124(3): 315-324.
    
21. Yoneda, A., Ito, T., Higaki, T., Kutsuna, N., Saito, T., Ishimizu, T., Osada, H., Hasezawa, S., Matsui, M. and Demura, T. (2010). Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules. Plant J 64:657-667.
    
22. Okubo-Kurihara, E., Higaki, T., Kurihara, Y., Kutsuna, N., Yamaguchi, J. and Hasezawa, S. (2011). Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture. J Plant Res 124(3): 395-403.
    
23. Higaki, T.*, Kojo, K. H. and Hasezawa, S. (2010). Critical role of actin bundling in plant cell morphogenesis. Plant Signal Behav 5(5): 484-488.
    
24. Higaki, T.*, Kutsuna, N., Sano, T., Kondo, N. and Hasezawa, S. (2010). Quantification and cluster analysis of actin cytoskeletal structures in plant cells: role of actin bundling in stomatal movement during diurnal cycles in Arabidopsis guard cells. Plant J 61(1): 156-165.
    
25. Nagano, A. J., Maekawa, A., Nakano, R. T., Miyahara, M., Higaki, T., Kutsuna, N. and Hasezawa, S. (2009). Hara-Nishimura I Quantitative analysis of ER body morphology in an Arabidopsis mutant. Plant Cell Physiol 50:2015-2022.
    
26. Okubo-Kurihara, E., Sano, T., Higaki, T., Kutsuna, N. and Hasezawa, S. (2009). Acceleration of vacuolar regeneration and cell growth by overexpression of an aquaporin NtTIP1;1 in tobacco BY-2 cells. Plant Cell Physiol 50(1): 151-160.
    
27. Oda, Y., Higaki, T., Hasezawa, S. and Kutsuna, N. (2009). New insights into plant vacuolar structure and dynamics. Int Rev Cell Mol Biol 277:103-135. (2009)
    
28. Higaki, T.*, Kutsuna, N., Sano, T. and Hasezawa, S. (2008). Quantitative analysis of changes in actin microfilament contribution to cell plate development in plant cytokinesis. BMC Plant Biol 8: 80.
    
29. Higaki, T.*, Kadota, Y., Goh, T., Hayashi, T., Kutsuna, N., Sano, T., Hasezawa, S. and Kuchitsu, K. (2008). Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells. Plant Signal Behav 3(9): 700-703.
    
30. Sano, T., Tanaka, Y., Higaki, T., Kutsuna, N. and Hasezawa, S. (2008). Dynamics of cytoskeletons and vacuoles in guard cells during stomatal movement. Floriculture, Ornamental and Plant Biotechnology (Global Science Books) 35: 329-333.
    
31. Higaki, T.*, Sano, T. and Hasezawa, S. (2007). Actin microfilament dynamics and actin side-binding proteins in plants. Curr Opin Plant Biol 10(6): 549-556.
    
32. Higaki, T.*, Goh, T., Hayashi, T., Kutsuna, N., Kadota, Y., Hasezawa, S., Sano, T. and Kuchitsu, K. (2007). Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: in vivo imaging of hypersensitive cell death in tobacco BY-2 cells. Plant Cell Physiol 48(10): 1414-1425.
    
33. Yoneda, A., Higaki, T., Kutsuna, N., Kondo, Y., Osada, H., Hasezawa, S. and Matsui, M. (2007). Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils. Plant Cell Physiol 48(10): 1393-1403.
    
34. Sano, T., Kutsuna, N., Higaki, T., Oda, Y., Yoneda, A., Kumagai-Sano, F. and Hasezawa, S. (2007). Cytoskeletal and vacuolar dynamics during plant cell division: Approaches using structure-visualized cells. Cell Division Control in Plants (Plant Cell Monogr (9)): 125-140.
    
35. Yoneda, A., Kutsuna, N., Higaki, T., Oda, Y., Sano, T. and Hasezawa, S. (2007). Recent progress in living cell imaging of plant cytoskeleton and vacuole using fluorescent-protein transgenic lines and three-dimensional imaging. Protoplasma 230(3-4): 129-139.
    
36. Gambe, A. E., Ono, R. M., Matsunaga, S., Kutsuna, N., Higaki, T., Higashi, T., Hasezawa, S., Uchiyama, S. and Fukui, K. (2007). Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics. Cytometry A 71(5): 286-296.
    
37. Higaki, T.*, Kutsuna, N., Okubo, E., Sano, T. and Hasezawa, S. (2006). Actin microfilaments regulate vacuolar structures and dynamics: dual observation of actin microfilaments and vacuolar membrane in living tobacco BY-2 Cells. Plant Cell Physiol 47(7): 839-852.
    
38. Sano, T., Higaki, T., Handa, K., Kadota, Y., Kuchitsu, K., Hasezawa, S., Hoffmann, A., Endter, J., Zimmermann, U., Hedrich, R. and Roitsch, T. (2006). Calcium ions are involved in the delay of plant cell cycle progression by abiotic stresses. FEBS Lett 580(2): 597-602.
    
39. Sano, T.*, Higaki, T.*, Oda, Y., Hayashi, T. and Hasezawa, S. (2005). Appearance of actin microfilament 'twin peaks' in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin. Plant J 44(4): 595-605.