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Darrell Cockburn

Education

Ph.D. in Molecular and Cellular Biology, the University of Guelph, Guelph, Ontario, 2010

Current position

Postdoctoral Fellow, the University of Michigan Department of Microbiology and Immunology (2014-present)

Publications

  1. Cockburn, D. W., Orlovsky, N. I., Foley, M. H., Kwiatkowski, K. J., Bahr, C. M., Maynard, M., Demeler, B. and Koropatkin, N. M. (2015). Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol 95(2): 209-230.
  2. Cockburn, D., Nielsen, M. M., Christiansen, C., Andersen, J. M., Rannes, J. B., Blennow, A. and Svensson, B. (2015). Surface binding sites in amylase have distinct roles in recognition of starch structure motifs and degradation. Int J Biol Macromol 75: 338-345.
  3. Wilkens, C., Cockburn, D., Andersen, S., Ole Petersen, B., Ruzanski, C., A. Field, R., Hindsgaul, O., Nakai, H., McCleary, B. and M. Smith, A. (2015). Analysis of Surface Binding Sites (SBS) within GH62, GH13 and GH77. Journal of Applied Glycoscience(0).
  4. Cockburn, D., Wilkens, C., Ruzanski, C., Andersen, S., Nielsen, J. W., Smith, A. M., Field, R. A., Willemoës, M., Hachem, M. A. and Svensson, B. (2014). Analysis of surface binding sites (SBSs) in carbohydrate active enzymes with focus on glycoside hydrolase families 13 and 77—a mini-review. Biologia 69(6): 705-712.
  5. Ruzanski, C., Smirnova, J., Rejzek, M., Cockburn, D., Pedersen, H. L., Pike, M., Willats, W. G., Svensson, B., Steup, M., Ebenhoh, O., Smith, A. M. and Field, R. A. (2013). A bacterial glucanotransferase can replace the complex maltose metabolism required for starch to sucrose conversion in leaves at night. J Biol Chem 288(40): 28581-28598.
  6. Cockburn, D. and Svensson, B. (2013). Surface binding sites in carbohydrate active enzymes: an emerging picture of structural and functional diversity. Carbohydrate Chemistry 39: 204-221.
  7. S. Møller, M., Cockburn, D., W. Nielsen, J., M. Jensen, J., B. Vester-Christensen, M., M. Nielsen, M., M. Andersen, J., Wilkens, C., Rannes, J. and Hägglund, P. (2013). Surface binding sites (SBSs), mechanism and regulation of enzymes degrading amylopectin and α-limit dextrins. Journal of applied glycoscience 60(2): 101-109.
  8. Diemer, S. K., Svensson, B., Babol, L. N., Cockburn, D., Grijpstra, P., Dijkhuizen, L., Folkenberg, D. M., Garrigues, C. and Ipsen, R. H. (2012). Binding Interactions Between α-glucans from Lactobacillus reuteri and Milk Proteins Characterised by Surface Plasmon Resonance. Food Biophysics 7(3): 220-226.
  9. Cockburn, D. W. and Clarke, A. J. (2011). Modulating the pH-activity profile of cellulase A from Cellulomonas fimi by replacement of surface residues. Protein Eng Des Sel 24(5): 429-437.
  10. Quirk, A., Lipkowski, J., Vandenende, C., Cockburn, D., Clarke, A. J., Dutcher, J. R. and Roscoe, S. G. (2010). Direct visualization of the enzymatic digestion of a single fiber of native cellulose in an aqueous environment by atomic force microscopy. Langmuir 26(7): 5007-5013.
  11. Cockburn, D. W., Vandenende, C. and Clarke, A. J. (2010). Modulating the pH-activity profile of cellulase by substitution: replacing the general base catalyst aspartate with cysteinesulfinate in cellulase A from Cellulomonas fimi. Biochemistry 49(9): 2042-2050.
  12. Jing, H., Cockburn, D., Zhang, Q. and Clarke, A. J. (2009). Production and purification of the isolated family 2a carbohydrate-binding module from Cellulomonas fimi. Protein Expr Purif 64(1): 63-68.
  13. Legaree, B. A., Daniels, K., Weadge, J. T., Cockburn, D. and Clarke, A. J. (2007). Function of penicillin-binding protein 2 in viability and morphology of Pseudomonas aeruginosa. J Antimicrob Chemother 59(3): 411-424.
Protocols by Darrell Cockburn
  1. Product Analysis of Starch Active Enzymes by TLC