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James A. Imlay


Ph.D. in Biochemistry, University of California at Berkeley, 1987

Current position

Professor, Department of Microbiology, University of Illinois at Urbana-Champaign

Publications (since 2010)

  1. James, A. I. (2015). Common mechanisms of bacterial metal homeostasis. In: Nriagu, J. O. and Skaar, E. P. (eds). Trace metals and infectious diseases. The MIT Press.
  2. Cavet, J. S., Perry, R. D., Brunke, S., Darwin, K. H., Fierke, C. A., Imlay, J. A., Murphy, M. E., Schryvers, A. B., Thiele, D. J. and Weiser, J. N. (2015). The microbe perspective. Trace Metals and Infectious Diseases: 99.
  3. Imlay, J. A. (2015). Diagnosing oxidative stress in bacteria: not as easy as you might think. Curr Opin Microbiol 24: 124-131.
  4. Martin, J. E., Waters, L. S., Storz, G. and Imlay, J. A. (2015). The Escherichia coli small protein MntS and exporter MntP optimize the intracellular concentration of manganese. PLoS Genet 11(3): e1004977.
  5. Mancini, S. and Imlay, J. A. (2015). The induction of two biosynthetic enzymes helps Escherichia coli sustain heme synthesis and activate catalase during hydrogen peroxide stress. Mol Microbiol 96(4): 744-763.
  6. Imlay, J. A. (2015). Transcription Factors That Defend Bacteria Against Reactive Oxygen Species. Annu Rev Microbiol.
  7. Imlay, K. R., Korshunov, S. and Imlay, J. A. (2015). The physiological roles and adverse effects of the two cystine importers of Escherichia coli. J Bacteriol.
  8. Sobota, J. M., Gu, M. and Imlay, J. A. (2014). Intracellular hydrogen peroxide and superoxide poison 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, the first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli. J Bacteriol 196(11): 1980-1991. (Cited by Faculty of 1000)
  9. Imlay, J. A. (2014). The mismetallation of enzymes during oxidative stress. J Biol Chem 289(41): 28121-28128.
  10. Liu, Y. and Imlay, J. A. (2013). Cell death from antibiotics without the involvement of reactive oxygen species. Science 339(6124): 1210-1213.
  11. Gu, M. and Imlay, J. A. (2013). Superoxide poisons mononuclear iron enzymes by causing mismetallation. Mol Microbiol 89(1): 123-134.
  12. Imlay, J. A. (2013). The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium. Nat Rev Microbiol 11(7): 443-454.
  13. Ravindra Kumar, S. and Imlay, J. A. (2013). How Escherichia coli tolerates profuse hydrogen peroxide formation by a catabolic pathway. J Bacteriol 195(20): 4569-4579.
  14. Singh, A. K., Shin, J. H., Lee, K. L., Imlay, J. A. and Roe, J. H. (2013). Comparative study of SoxR activation by redox-active compounds. Mol Microbiol 90(5): 983-996.
  15. Mishra, S. and Imlay, J. A. (2013). An anaerobic bacterium, Bacteroides thetaiotaomicron, uses a consortium of enzymes to scavenge hydrogen peroxide. Mol Microbiol 90(6): 1356-1371.
  16. Xu, F. F. and Imlay, J. A. (2012). Silver(I), mercury(II), cadmium(II), and zinc(II) target exposed enzymic iron-sulfur clusters when they toxify Escherichia coli. Appl Environ Microbiol 78(10): 3614-3621.
  17. Anjem, A. and Imlay, J. A. (2012). Mononuclear iron enzymes are primary targets of hydrogen peroxide stress. J Biol Chem 287(19): 15544-15556.
  18. Mishra, S. and Imlay, J. (2012). Why do bacteria use so many enzymes to scavenge hydrogen peroxide? Archives of biochemistry and biophysics 525(2): 145-160.
  19. Arenas, F. A., Covarrubias, P. C., Sandoval, J. M., Perez-Donoso, J. M., Imlay, J. A. and Vasquez, C. C. (2011). The Escherichia coli BtuE protein functions as a resistance determinant against reactive oxygen species. PLoS One 6(1): e15979.
  20. Gu, M. and Imlay, J. A. (2011). The SoxRS response of Escherichia coli is directly activated by redox-cycling drugs rather than by superoxide. Mol Microbiol 79(5): 1136-1150.
  21. Imlay, J. A., Hassett, D. J. and Kidd, S. (2011). 1 Oxidative and Nitrosative Stress Defence Systems in Escherichia coli and Pseudomonas aeruginosa: A Model Organism of Study Versus a Human Opportunistic Pathogen. Stress Response in Pathogenic Bacteria 19: 1.
  22. Imlay, J. A. (2011). Redox pioneer: professor Irwin Fridovich. Antioxid Redox Signal 14(3): 335-340.
  23. Martin, J. E. and Imlay, J. A. (2011). The alternative aerobic ribonucleotide reductase of Escherichia coli, NrdEF, is a manganese-dependent enzyme that enables cell replication during periods of iron starvation. Mol Microbiol 80(2): 319-334.
  24. Liu, Y., Bauer, S. C. and Imlay, J. A. (2011). The YaaA protein of the Escherichia coli OxyR regulon lessens hydrogen peroxide toxicity by diminishing the amount of intracellular unincorporated iron. J Bacteriol 193(9): 2186-2196.
  25. Sobota, J. M. and Imlay, J. A. (2011). Iron enzyme ribulose-5-phosphate 3-epimerase in Escherichia coli is rapidly damaged by hydrogen peroxide but can be protected by manganese. Proc Natl Acad Sci U S A 108(13): 5402-5407.
  26. Carey, J. R., Suslick, K. S., Hulkower, K. I., Imlay, J. A., Imlay, K. R., Ingison, C. K., Ponder, J. B., Sen, A. and Wittrig, A. E. (2011). Rapid identification of bacteria with a disposable colorimetric sensing array. J Am Chem Soc 133(19): 7571-7576.
  27. Imlay, J. and Gu, M. (2011). Many plants and bacteria excrete redox-cycling compounds. Free Radic Biol Med 50(12): 1814-1815.
  28. Korshunov, S. and Imlay, J. A. (2010). Two sources of endogenous hydrogen peroxide in Escherichia coli. Mol Microbiol 75(6): 1389-1401.
  29. Yeom, J., Imlay, J. A. and Park, W. (2010). Iron homeostasis affects antibiotic-mediated cell death in Pseudomonas species. J Biol Chem 285(29): 22689-22695.
  30. Arenas, F. A., Diaz, W. A., Leal, C. A., Perez-Donoso, J. M., Imlay, J. A. and Vasquez, C. C. (2010). The Escherichia coli btuE gene, encodes a glutathione peroxidase that is induced under oxidative stress conditions. Biochem Biophys Res Commun 398(4): 690-694.
  31. Wu, P., Imlay, J. A. and Shang, J. K. (2010). Mechanism of Escherichia coli inactivation on palladium-modified nitrogen-doped titanium dioxide. Biomaterials 31(29): 7526-7533.
  32. Salvail, H., Lanthier-Bourbonnais, P., Sobota, J. M., Caza, M., Benjamin, J.-A. M., Mendieta, M. E. S., Lépine, F., Dozois, C. M., Imlay, J. and Massé, E. (2010). A small RNA promotes siderophore production through transcriptional and metabolic remodeling. Proceedings of the National Academy of Sciences 107(34): 15223-15228.
  33. Wu, P., Xie, R., Imlay, K. and Shang, J. K. (2010). Visible-light-induced bactericidal activity of titanium dioxide codoped with nitrogen and silver. Environmental science & technology 44(18): 6992-6997.
  34. Jang, S. and Imlay, J. A. (2010). Hydrogen peroxide inactivates the Escherichia coli Isc iron-sulphur assembly system, and OxyR induces the Suf system to compensate. Mol Microbiol 78(6): 1448-1467.
Protocols by James A. Imlay
  1. Bacterial Porphyrin Extraction and Quantification by LC/MS/MS Analysis