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Jason F. Huntley


Ph.D. in Molecular and Cellular Pathology, Department of Veterinary Pathology, Iowa State University, Ames, Iowa, 2004

Current position

Assistant Professor and BSL3 Director, Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio


  1. LoVullo, E. D., Wright, L. F., Isabella, V., Huntley, J. F. and Pavelka, M. S., Jr. (2015). Revisiting the Gram-negative lipoprotein paradigm. J Bacteriol 197(10): 1705-1715.
  2. Curtis, M. M., Russell, R., Moreira, C. G., Adebesin, A. M., Wang, C., Williams, N. S., Taussig, R., Stewart, D., Zimmern, P., Lu, B., Prasad, R. N., Zhu, C., Rasko, D. A., Huntley, J. F., Falck, J. R. and Sperandio, V. (2014). QseC inhibitors as an antivirulence approach for Gram-negative pathogens. MBio 5(6): e02165.
  3. Ren, G., Champion, M. M. and Huntley, J. F. (2014). Identification of disulfide bond isomerase substrates reveals bacterial virulence factors. Mol Microbiol 94(4): 926-944.
  4. Mahawar, M., Atianand, M. K., Dotson, R. J., Mora, V., Rabadi, S. M., Metzger, D. W., Huntley, J. F., Harton, J. A., Malik, M. and Bakshi, C. S. (2012). Identification of a novel Francisella tularensis factor required for intramacrophage survival and subversion of innate immune response. J Biol Chem 287(30): 25216-25229.
  5. Huntley, J. F., Robertson, G. T. and Norgard, M. V. (2010). Method for the isolation of Francisella tularensis outer membranes. J Vis Exp(40).
  6. Kiss, K., Liu, W., Huntley, J. F., Norgard, M. V. and Hansen, E. J. (2008). Characterization of fig operon mutants of Francisella novicida U112. FEMS Microbiol Lett 285(2): 270-277.
  7. Rasko, D. A., Moreira, C. G., Li de, R., Reading, N. C., Ritchie, J. M., Waldor, M. K., Williams, N., Taussig, R., Wei, S., Roth, M., Hughes, D. T., Huntley, J. F., Fina, M. W., Falck, J. R. and Sperandio, V. (2008). Targeting QseC signaling and virulence for antibiotic development. Science 321(5892): 1078-1080.
  8. Huntley, J. F., Conley, P. G., Rasko, D. A., Hagman, K. E., Apicella, M. A. and Norgard, M. V. (2008). Native outer membrane proteins protect mice against pulmonary challenge with virulent type A Francisella tularensis. Infect Immun 76(8): 3664-3671.
  9. Huntley, J. F., Conley, P. G., Hagman, K. E. and Norgard, M. V. (2007). Characterization of Francisella tularensis outer membrane proteins. J Bacteriol 189(2): 561-574.
  10. Huntley, J. F., Stabel, J. R. and Bannantine, J. P. (2005). Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein. BMC Microbiol 5: 3.
  11. Huntley, J. F., Whitlock, R. H., Bannantine, J. P. and Stabel, J. R. (2005). Comparison of diagnostic detection methods for Mycobacterium avium subsp. paratuberculosis in North American bison. Vet Pathol 42(1): 42-51.
  12. Huntley, J. F., Stabel, J. R., Paustian, M. L., Reinhardt, T. A. and Bannantine, J. P. (2005). Expression library immunization confers protection against Mycobacterium avium subsp. paratuberculosis infection. Infect Immun 73(10): 6877-6884.
  13. Bannantine, J. P., Huntley, J. F., Miltner, E., Stabel, J. R. and Bermudez, L. E. (2003). The Mycobacterium avium subsp. paratuberculosis 35 kDa protein plays a role in invasion of bovine epithelial cells. Microbiology 149(Pt 8): 2061-2069.
Protocols by Jason F. Huntley
  1. Generating Isogenic Deletions (Knockouts) in Francisella tularensis, a Highly-infectious and Fastidious Gram-negative Bacterium