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Benoit Stijlemans


Ph.D. in Applied Biological Sciences (with the grade of greatest distinction), Vrije Universiteit Brussel (VUB), 2006

Current position

Senior Post-doctoral researcher VUB, Vrije Universiteit Brussel (VUB) (2014-current)

Publications (since 2005)

Peer review publications

  1. Stijlemans, B., Cnops, J., Naniima, P., Vaast, A., Bockstal, V., De Baetselier, P. and Magez, S. (2015). Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis. PLoS Negl Trop Dis 9(3): e0003561.
  2. Van Gassen, N., Van Overmeire, E., Leuckx, G., Heremans, Y., De Groef, S., Cai, Y., Elkrim, Y., Gysemans, C., Stijlemans, B., Van de Casteele, M., De Baetselier, P., De Leu, N., Heimberg, H. and Van Ginderachter, J. A. (2015). Macrophage dynamics are regulated by local macrophage proliferation and monocyte recruitment in injured pancreas. Eur J Immunol 45(5): 1482-1493.
  3. Obishakin, E., Stijlemans, B., Santi-Rocca, J., Vandenberghe, I., Devreese, B., Muldermans, S., Bastin, P. and Magez, S. (2014). Generation of a nanobody targeting the paraflagellar rod protein of trypanosomes. PLoS One 9(12): e115893.
  4. Zheng, F., Devoogdt, N., Sparkes, A., Morias, Y., Abels, C., Stijlemans, B., Lahoutte, T., Muyldermans, S., De Baetselier, P., Schoonooghe, S., Beschin, A. and Raes, G. (2015). Monitoring liver macrophages using nanobodies targeting Vsig4: concanavalin A induced acute hepatitis as paradigm. Immunobiology 220(2): 200-209.
  5. Stijlemans, B., Leng, L., Brys, L., Sparkes, A., Vansintjan, L., Caljon, G., Raes, G., Van Den Abbeele, J., Van Ginderachter, J. A., Beschin, A., Bucala, R. and De Baetselier, P. (2014). MIF contributes to Trypanosoma brucei associated immunopathogenicity development. PLoS Pathog 10(9): e1004414.
  6. La Greca, F., Haynes, C., Stijlemans, B., De Trez, C. and Magez, S. (2014). Antibody-mediated control of Trypanosoma vivax infection fails in the absence of tumour necrosis factor. Parasite Immunol 36(6): 271-276.
  7. Nikolaou, A., Stijlemans, B., Laoui, D., Schouppe, E., Tran, H. T., Tourwe, D., Chai, S. Y., Vanderheyden, P. M. and Van Ginderachter, J. A. (2014). Presence and regulation of insulin-regulated aminopeptidase in mouse macrophages. J Renin Angiotensin Aldosterone Syst 15(4): 466-479.
  8. Matthijs, S., Ye, L., Stijlemans, B., Cornelis, P., Bossuyt, F. and Roelants, K. (2014). Low structural variation in the host-defense peptide repertoire of the dwarf clawed frog Hymenochirus boettgeri (Pipidae). PLoS One 9(1): e86339.
  9. Braem, G., Stijlemans, B., Van Haken, W., De Vliegher, S., De Vuyst, L. and Leroy, F. (2014). Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens. J Appl Microbiol 116(5): 1084-1093.
  10. De Muylder, G., Daulouede, S., Lecordier, L., Uzureau, P., Morias, Y., Van Den Abbeele, J., Caljon, G., Herin, M., Holzmuller, P., Semballa, S., Courtois, P., Vanhamme, L., Stijlemans, B., De Baetselier, P., Barrett, M. P., Barlow, J. L., McKenzie, A. N., Barron, L., Wynn, T. A., Beschin, A., Vincendeau, P. and Pays, E. (2013). A Trypanosoma brucei kinesin heavy chain promotes parasite growth by triggering host arginase activity. PLoS Pathog 9(10): e1003731.
  11. Roelants, K., Fry, B. G., Ye, L., Stijlemans, B., Brys, L., Kok, P., Clynen, E., Schoofs, L., Cornelis, P. and Bossuyt, F. (2013). Origin and functional diversification of an amphibian defense peptide arsenal. PLoS Genet 9(8): e1003662.
  12. Caljon, G., Stijlemans, B., Saerens, D., Van Den Abbeele, J., Muyldermans, S., Magez, S. and De Baetselier, P. (2012). Affinity is an important determinant of the anti-trypanosome activity of nanobodies. PLoS Negl Trop Dis 6(11): e1902.
  13. Caljon, G., De Ridder, K., Stijlemans, B., Coosemans, M., Magez, S., De Baetselier, P. and Van Den Abbeele, J. (2012). Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity. PLoS One 7(10): e47233.
  14. Korf, H., Wenes, M., Stijlemans, B., Takiishi, T., Robert, S., Miani, M., Eizirik, D. L., Gysemans, C. and Mathieu, C. (2012). 1,25-Dihydroxyvitamin D3 curtails the inflammatory and T cell stimulatory capacity of macrophages through an IL-10-dependent mechanism. Immunobiology 217(12): 1292-1300.
  15. Shahrooei, M., Hira, V., Khodaparast, L., Khodaparast, L., Stijlemans, B., Kucharikova, S., Burghout, P., Hermans, P. W. and Van Eldere, J. (2012). Vaccination with SesC decreases Staphylococcus epidermidis biofilm formation. Infect Immun 80(10): 3660-3668.
  16. De Vooght, L., Caljon, G., Stijlemans, B., De Baetselier, P., Coosemans, M. and Van den Abbeele, J. (2012). Expression and extracellular release of a functional anti-trypanosome Nanobody(R) in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact 11: 23.
  17. Caljon, G., Caveliers, V., Lahoutte, T., Stijlemans, B., Ghassabeh, G. H., Van Den Abbeele, J., Smolders, I., De Baetselier, P., Michotte, Y., Muyldermans, S., Magez, S. and Clinckers, R. (2012). Using microdialysis to analyse the passage of monovalent nanobodies through the blood-brain barrier. Br J Pharmacol 165(7): 2341-2353.
  18. Pletinckx, K., Stijlemans, B., Pavlovic, V., Laube, R., Brandl, C., Kneitz, S., Beschin, A., De Baetselier, P. and Lutz, M. B. (2011). Similar inflammatory DC maturation signatures induced by TNF or Trypanosoma brucei antigens instruct default Th2-cell responses. Eur J Immunol 41(12): 3479-3494.
  19. Bosschaerts, T., Morias, Y., Stijlemans, B., Herin, M., Porta, C., Sica, A., Mantovani, A., De Baetselier, P. and Beschin, A. (2011). IL-10 limits production of pathogenic TNF by M1 myeloid cells through induction of nuclear NF-kappaB p50 member in Trypanosoma congolense infection-resistant C57BL/6 mice. Eur J Immunol 41(11): 3270-3280.
  20. Stijlemans, B., Caljon, G., Natesan, S. K., Saerens, D., Conrath, K., Perez-Morga, D., Skepper, J. N., Nikolaou, A., Brys, L., Pays, E., Magez, S., Field, M. C., De Baetselier, P. and Muyldermans, S. (2011). High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosis. PLoS Pathog 7(6): e1002072.
  21. Vankrunkelsven, A., De Ceulaer, K., Hsu, D., Liu, F. T., De Baetselier, P. and Stijlemans, B. (2010). Lack of galectin-3 alleviates trypanosomiasis-associated anemia of inflammation. Immunobiology 215(9-10): 833-841.
  22. Bosschaerts, T., Guilliams, M., Stijlemans, B., Morias, Y., Engel, D., Tacke, F., Herin, M., De Baetselier, P. and Beschin, A. (2010). Tip-DC development during parasitic infection is regulated by IL-10 and requires CCL2/CCR2, IFN-gamma and MyD88 signaling. PLoS Pathog 6(8): e1001045.
  23. Magez, S., Caljon, G., Tran, T., Stijlemans, B. and Radwanska, M. (2010). Current status of vaccination against African trypanosomiasis. Parasitology 137(14): 2017-2027.
  24. Stijlemans, B., Vankrunkelsven, A., Brys, L., Raes, G., Magez, S. and De Baetselier, P. (2010). Scrutinizing the mechanisms underlying the induction of anemia of inflammation through GPI-mediated modulation of macrophage activation in a model of African trypanosomiasis. Microbes Infect 12(5): 389-399.
  25. Stijlemans, B., Vankrunkelsven, A., Caljon, G., Bockstal, V., Guilliams, M., Bosschaerts, T., Beschin, A., Raes, G., Magez, S. and De Baetselier, P. (2010). The central role of macrophages in trypanosomiasis-associated anemia: rationale for therapeutical approaches. Endocr Metab Immune Disord Drug Targets 10(1): 71-82. (Review)
  26. Gomez-Rodriguez, J., Stijlemans, B., De Muylder, G., Korf, H., Brys, L., Berberof, M., Darji, A., Pays, E., De Baetselier, P. and Beschin, A. (2009). Identification of a parasitic immunomodulatory protein triggering the development of suppressive M1 macrophages during African trypanosomiasis. J Infect Dis 200(12): 1849-1860.
  27. Bosschaerts, T., Guilliams, M., Stijlemans, B., De Baetselier, P. and Beschin, A. (2009). Understanding the role of monocytic cells in liver inflammation using parasite infection as a model. Immunobiology 214(9-10): 737-747. (Review)
  28. Shahrooei, M., Hira, V., Stijlemans, B., Merckx, R., Hermans, P. W. and Van Eldere, J. (2009). Inhibition of Staphylococcus epidermidis biofilm formation by rabbit polyclonal antibodies against the SesC protein. Infect Immun 77(9): 3670-3678.
  29. Korf, H., Vander Beken, S., Romano, M., Steffensen, K. R., Stijlemans, B., Gustafsson, J. A., Grooten, J. and Huygen, K. (2009). Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice. J Clin Invest 119(6): 1626-1637.
  30. Muyldermans, S., Baral, T. N., Retamozzo, V. C., De Baetselier, P., De Genst, E., Kinne, J., Leonhardt, H., Magez, S., Nguyen, V. K., Revets, H., Rothbauer, U., Stijlemans, B., Tillib, S., Wernery, U., Wyns, L., Hassanzadeh-Ghassabeh, G. and Saerens, D. (2009). Camelid immunoglobulins and nanobody technology. Vet Immunol Immunopathol 128(1-3): 178-183.
  31. Stijlemans, B., Vankrunkelsven, A., Brys, L., Magez, S. and De Baetselier, P. (2008). Role of iron homeostasis in trypanosomiasis-associated anemia. Immunobiology 213(9-10): 823-835.
  32. Saerens, D., Stijlemans, B., Baral, T. N., Nguyen Thi, G. T., Wernery, U., Magez, S., De Baetselier, P., Muyldermans, S. and Conrath, K. (2008). Parallel selection of multiple anti-infectome Nanobodies without access to purified antigens. J Immunol Methods 329(1-2): 138-150.
  33. Stijlemans, B., Baral, T. N., Guilliams, M., Brys, L., Korf, J., Drennan, M., Van Den Abbeele, J., De Baetselier, P. and Magez, S. (2007). A glycosylphosphatidylinositol-based treatment alleviates trypanosomiasis-associated immunopathology. J Immunol 179(6): 4003-4014.
  34. Stijlemans, B., Guilliams, M., Raes, G., Beschin, A., Magez, S. and De Baetselier, P. (2007). African trypanosomosis: from immune escape and immunopathology to immune intervention. Vet Parasitol 148(1): 3-13.
  35. Caljon, G., Van Den Abbeele, J., Stijlemans, B., Coosemans, M., De Baetselier, P. and Magez, S. (2006). Tsetse fly saliva accelerates the onset of Trypanosoma brucei infection in a mouse model associated with a reduced host inflammatory response. Infect Immun 74(11): 6324-6330.
  36. Prochazkova, P., Silerova, M., Stijlemans, B., Dieu, M., Halada, P., Joskova, R., Beschin, A., De Baetselier, P. and Bilej, M. (2006). Evidence for proteins involved in prophenoloxidase cascade Eisenia fetida earthworms. J Comp Physiol B 176(6): 581-587.
  37. Baral, T. N., Magez, S., Stijlemans, B., Conrath, K., Vanhollebeke, B., Pays, E., Muyldermans, S. and De Baetselier, P. (2006). Experimental therapy of African trypanosomiasis with a nanobody-conjugated human trypanolytic factor. Nat Med 12(5): 580-584.
  38. Drennan, M. B., Stijlemans, B., Van den Abbeele, J., Quesniaux, V. J., Barkhuizen, M., Brombacher, F., De Baetselier, P., Ryffel, B. and Magez, S. (2005). The induction of a type 1 immune response following a Trypanosoma brucei infection is MyD88 dependent. J Immunol 175(4): 2501-2509.
  39. Conrath, K., Vincke, C., Stijlemans, B., Schymkowitz, J., Decanniere, K., Wyns, L., Muyldermans, S. and Loris, R. (2005). Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH. J Mol Biol 350(1): 112-125.
Protocols by Benoit Stijlemans
  1. Murine Liver Myeloid Cell Isolation Protocol