Education

Ph.D. in Biology, Department of Plant Physiology, University of Granada, 2001

Current position

Tenured Scientist in Plant Biology, Department Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain

Publications (since 2005)

1. Zienkiewicz, K., Alche, J. D., Zienkiewicz, A., Tormo, A. and Castro, A. J. (2015). Identification of olive pollen allergens using a fluorescence-based 2D multiplex method. Electrophoresis. (Epub ahead of print)
   
2. Zienkiewicz, A., Zienkiewicz, K., Florido, F., Marco, F. M., Romero, P. J., Castro, A. J. and de Dios Alche, J. (2014). Chip-based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy. Electrophoresis 35(18): 2681-2685.
   
3. Zienkiewicz, A., Rejon, J. D., de Dios Alche, J., Rodriguez-Garcia, M. I. and Castro, A. J.* (2014). A protocol for protein extraction from lipid-rich plant tissues suitable for electrophoresis. Methods Mol Biol 1072: 85-91.
   
4. Rejon, J. D., Delalande, F., Schaeffer-Reiss, C., Carapito, C., Zienkiewicz, K., de Dios Alche, J., Isabel Rodriguez-Garcia, M., Van Dorsselaer, A. and Castro, A. J. (2014). The plant stigma exudate: a biochemically active extracellular environment for pollen germination? Plant Signal Behav 9(3): e28274.
   
5. Zienkiewicz, A., Zienkiewicz, K., Rejon, J. D., Alche Jde, D., Castro, A. J. and Rodriguez-Garcia, M. I. (2014). Olive seed protein bodies store degrading enzymes involved in mobilization of oil bodies. J Exp Bot 65(1): 103-115.
   
6. Rejon, J. D., Delalande, F., Schaeffer-Reiss, C., Carapito, C., Zienkiewicz, K., de Dios Alche, J., Rodriguez-Garcia, M. I., Van Dorsselaer, A. and Castro, A. J. (2013). Proteomics profiling reveals novel proteins and functions of the plant stigma exudate. J Exp Bot 64(18): 5695-5705.
   
7. Castro, A. J., Suarez, C., Zienkiewicz, K., Alche Jde, D., Zienkiewicz, A. and Rodriguez-Garcia, M. I. (2013). Electrophoretic profiling and immunocytochemical detection of pectins and arabinogalactan proteins in olive pollen during germination and pollen tube growth. Ann Bot 112(3): 503-513.
   
8. Suarez, C., Zienkiewicz, A., Castro, A. J., Zienkiewicz, K., Majewska-Sawka, A. and Rodriguez-Garcia, M. I. (2013). Cellular localization and levels of pectins and arabinogalactan proteins in olive (Olea europaea L.) pistil tissues during development: implications for pollen-pistil interaction. Planta 237(1): 305-319.
   
9. Soleimani, A., Morales, S., Jimenez-Lopez, J. C., Castro, A. J., Rodríguez-García, M. I. and de Dios Alché, J. (2013). Differential expression and sequence polymorphism of the olive pollen allergen Ole e 1 in two Iranian cultivars. Iranian Journal of Allergy, Asthma and Immunology 12(1): 18-28.
   
10. Zienkiewicz, A., Zienkiewicz, K., Rejon, J. D., Rodriguez-Garcia, M. I. and Castro, A. J. (2013). New insights into the early steps of oil body mobilization during pollen germination. J Exp Bot 64(1): 293-302.
    
11. Jimenez-Lopez, J. C., Morales, S., Castro, A. J., Volkmann, D., Rodriguez-Garcia, M. I. and Alche, J. D. (2012). Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS One 7(2): e30878.
    
12. Morales, S., Castro, A. J., Jimenez-Lopez, J. C., Florido, F., Rodriguez-Garcia, M. I. and de Dios Alche, J. (2012). A novel multiplex method for the simultaneous detection and relative quantitation of pollen allergens. Electrophoresis 33(9-10): 1367-1374.
    
13. Bal, U., Shariatpanahi, M. E., Castro, A. J., Emery, D., Clément, C., Dehestani-Ardakani, M., Mozaffari, K. and Touraev, A. (2012). Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide. Czech J of Genet Plant.
    
14. Rodríguez-García, M. I., Alché, J., Castro, A., Suarez, C. and Rejón, J. D. (2010). Pollen and pistil features involved in the reproductive biology of the olive (Olea europaea L.). XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Plant 932.
    
15. Soleimani, A., Alché, J., Castro, A., Rodríguez-García, M. I. and Ladan Moghadam, A. (2010). Using two-dimensional gel electrophoresis approach for characterizing of the Ole e 1, an olive pollen major allergen. XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Plant 932.
    
16. Zienkiewicz, A., Castro, A. J., Zienkiewicz, K., Caracuel, D. G., Cogolludo, A. M., Enríquez, C., Morales, J. C., Ruiz-Gámez, I., Ruíz-Maldonado, M. V., Torreblanca, S., Vicente, G. and Alché, J. D. (2012). Reproductive biology research in Cucurbitaceae by high school students. In: Méndez-Vilas, A. (ed). Current microscopy contributions to advances in science and technology. Formatex Research Center, 982-987.
    
17. Zienkiewicz, K., García-Quirós, E., Alché, J. D., Rodríguez-García, M. I. and Castro, A. J.* (2012). Detection and quantitation of olive pollen allergen isoforms using 2-D Western blotting. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. InTech Publishers, 41-56.
    
18. Morales, S., Castro, A. J., Salmerón, C., Marco, F., Rodríguez-García, M. I. and Alché, J. D. (2012). Clustering of olive pollens into model cultivars on the basis of their allergenic content. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. InTech Publishers, 27-40.
    
19. Morales, S., Castro, A. J., Rodríguez-García, M. I. and Alché, J. D. (2012). Involvement of climatic factors over the allergen expression in olive pollen. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. InTech Publishers, 57-70.
    
20. Alché, J. D., Zafra, A., Jiménez-López, J. C., Morales, S., Castro, A. J., Florido, F. and Rodríguez-García, M. I. (2012). Pollen allergenicity is highly dependent on the plant genetic background: the “cultivar” issue. In: Jiménez-López, J. C. (ed). Current insights in pollen allergens. InTech Publishers, 1-26.
    
21. Jiménez-López, J. C., Kotchoni, S. O., Gachomo, E. W., Castro, A. J., Rodríguez-García, M. I. and Alché, J. D. (2012). Molecular features of maize allergens and their implications in human health. In: Jiménez-López, J. C (ed). Maize: cultivation, uses and health benefits. Nova Science Publishers Inc., 137-178.
    
22. Rejon, J. D., Zienkiewicz, A., Rodriguez-Garcia, M. I. and Castro, A. J. (2012). Profiling and functional classification of esterases in olive (Olea europaea) pollen during germination. Ann Bot 110(5): 1035-1045.
    
23. Suarez, C., Castro, A. J., Rapoport, H. F. and Rodriguez-Garcia, M. I. (2012). Morphological, histological and ultrastructural changes in the olive pistil during flowering. Sex Plant Reprod 25(2): 133-146.
    
24. Zienkiewicz, K., Rejon, J. D., Suarez, C., Castro, A. J., de Dios Alche, J. and Rodriguez Garcia, M. I. (2011). Whole-organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant reproduction. BMC Plant Biol 11: 150.
    
25. Zienkiewicz, K., Zienkiewicz, A., Rodriguez-Garcia, M. I. and Castro, A. J. (2011). Characterization of a caleosin expressed during olive (Olea europaea L.) pollen ontogeny. BMC Plant Biol 11: 122.
    
26. Castro, A. J., Bednarczyk, A., Schaeffer-Reiss, C., Rodriguez-Garcia, M. I., Van Dorsselaer, A. and Alche, J. D. (2010). Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis. Proteomics 10(5): 953-962.
    
27. Castro, A. J., Rejón, J. D., Fendri, M., Jiménez-Quesada, M. J., Zafra, A., Jiménez-López, J. C., Rodríguez-García, M. I. and Alché, J. D. (2010). Taxonomical discrimination of pollen grains by using confocal laser scanning microscopy (CLSM) imaging of autofluorescence. In: Méndez-Vilas, A. and Díaz, J. (eds). Microscopy: Science, Technology, Applications and Education. Microscopy Book Series, Number 4. Formatex Publishers, 607-613.
    
28. Zienkiewicz, K., Castro, A. J., Alche Jde, D., Zienkiewicz, A., Suarez, C. and Rodriguez-Garcia, M. I. (2010). Identification and localization of a caleosin in olive (Olea europaea L.) pollen during in vitro germination. J Exp Bot 61(5): 1537-1546.
    
29. Castro, A. J., Saladin, G., Bezier, A., Mazeyrat-Gourbeyre, F., Baillieul, F. and Clement, C. (2008). The herbicide flumioxazin stimulates pathogenesis-related gene expression and enzyme activities in Vitis vinifera. Physiol Plant 134(3): 453-463.
    
30. Cilindre, C., Jegou, S., Hovasse, A., Schaeffer, C., Castro, A. J., Clement, C., Van Dorsselaer, A., Jeandet, P. and Marchal, R. (2008). Proteomic approach to identify champagne wine proteins as modified by Botrytis cinerea infection. J Proteome Res 7(3): 1199-1208.
    
31. Hamman-Khalifa, A., Castro, A. J., Jimenez-Lopez, J. C., Rodriguez-Garcia, M. I. and Alche Jde, D. (2008). Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen. BMC Plant Biol 8: 10.
    
32. Morales, S., Jimenez-Lopez, J. C., Castro, A. J., Rodriguez-Garcia, M. I. and Alche, J. D. (2008). Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination. J Microsc 231(2): 332-341.
    
33. Alché Ramírez, J. d. D., Castro López, A. J., Jiménez-López, J. C., Morales, S., Zafra, A., Hamman-Khalifa, A. M. and Rodríguez García, M. I. (2007). Differential characteristics of olive pollen from different cultivars: biological and clinical implications.
    
34. Castro, A. J., Alche, J. D., Calabozo, B., Rodriguez-Garcia, M. I. and Polo, F. (2007). Pla 1 1 and Ole e 1 pollen allergens share common epitopes and similar ultrastructural localization. J Investig Allergol Clin Immunol 17 Suppl 1: 41-47.
    
35. Castro, A. J. and Clement, C. (2007). Sucrose and starch catabolism in the anther of Lilium during its development: a comparative study among the anther wall, locular fluid and microspore/pollen fractions. Planta 225(6): 1573-1582.
    
36. Cilindre, C., Castro, A. J., Clément, C., Jeandet, P. and Marchal, R. (2007). Use of two-dimensional electrophoresis and immunodetection to characterize changes in the total protein content of Champagne base wine due to Botrytis cinerea infection. In: Jeandet, P., Clément, C. and Conreux, A. (eds). Macromolecules and Secondary Metabolites of Grapevine and Wine. Intercept-Lavoisier, 197-204.
    
37. Cilindre, C., Castro, A. J., Clément, C., Jeandet, P. and Marchal, R. (2007). Influence of Botrytis cinerea infection on Champagne wine proteins (characterized by two-dimensional electrophoresis/immunodetection) and wine foaming properties. Food Chem 103(1): 139-149.
    
38. de Dios Alche, J., Jimenez-Lopez, J. C., Wang, W., Castro-Lopez, A. J. and Rodriguez-Garcia, M. I. (2006). Biochemical characterization and cellular localization of 11S type storage proteins in olive (Olea europaea L.) seeds. J Agric Food Chem 54(15): 5562-5570.
    
39. Castro, A. J., Carapito, C., Zorn, N., Magne, C., Leize, E., Van Dorsselaer, A. and Clement, C. (2005). Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress. J Exp Bot 56(421): 2783-2795.