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Pilar Testillano

Current position

Scientific Researcher of CSIC (Permanent staff), Head of group Pollen Biotechnology of Crop Plants, Biological Research Center (Centro de Investigaciones Biológicas, CIB), Department of Environmental Biology, Spanish National Research Council (Consejo Superior de Investigaciones Científicas, CSIC), 04/1996

Publications (since 2010)

  1. Pereira, A. M., Masiero, S., Nobre, M. S., Costa, M. L., Solis, M. T., Testillano, P. S., Sprunck, S. and Coimbra, S. (2014). Differential expression patterns of arabinogalactan proteins in Arabidopsis thaliana reproductive tissues. J Exp Bot 65(18): 5459-5471.
  2. Rodriguez-Sanz, H., Manzanera, J. A., Solis, M. T., Gomez-Garay, A., Pintos, B., Risueno, M. C. and Testillano, P. S. (2014). Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suber L. BMC Plant Biol 14: 224.
  3. Rodriguez-Sanz, H., Moreno-Romero, J., Solis, M. T., Kohler, C., Risueno, M. C. and Testillano, P. S. (2014). Changes in histone methylation and acetylation during microspore reprogramming to embryogenesis occur concomitantly with Bn HKMT and Bn HAT expression and are associated with cell totipotency, proliferation, and differentiation in Brassica napus. Cytogenet Genome Res 143(1-3): 209-218.
  4. Rispail, N., De Matteis, L., Santos, R., Miguel, A. S., Custardoy, L., Testillano, P. S., Risueno, M. C., Perez-de-Luque, A., Maycock, C., Fevereiro, P., Oliva, A., Fernandez-Pacheco, R., Ibarra, M. R., de la Fuente, J. M., Marquina, C., Rubiales, D. and Prats, E. (2014). Quantum dot and superparamagnetic nanoparticle interaction with pathogenic fungi: internalization and toxicity profile. ACS Appl Mater Interfaces 6(12): 9100-9110.
  5. El-Tantawy, A. A., Solis, M. T., Risueno, M. C. and Testillano, P. S. (2014). Changes in DNA methylation levels and nuclear distribution patterns after microspore reprogramming to embryogenesis in barley. Cytogenet Genome Res 143(1-3): 200-208.
  6. Solis, M. T., Chakrabarti, N., Corredor, E., Cortes-Eslava, J., Rodriguez-Serrano, M., Biggiogera, M., Risueno, M. C. and Testillano, P. S. (2014). Epigenetic changes accompany developmental programmed cell death in tapetum cells. Plant Cell Physiol 55(1): 16-29.
  7. Rodriguez-Sanz, H., Solis, M. T., Lopez, M. F., Gomez –Cadenas, A., Risueno, M. C. and Testillano, P. S. (2014). Dynamics of auxin concentration, distribution and transport, and NIT2 expression reveal their involvement in microspore embryogenesis. In: Hozak, P. (ed). Microscopy for Global Challenges, IMC14 Proceedings. Czech Academy of Sciences Publishing, LS-8-O-3516.
  8. Solis, M. T., Barany, I., Cano, V., Risueno, M. C. and Testillano, P. S. (2014). Autophagic hallmarks and gene expression accompany programmed cell death in stressinduced pollen embryogenesis and pollen development. In: Hozak, P. (ed). Microscopy for Global Challenges, IMC14 Proceedings. Czech Academy of Sciences Publishing. CABI, LS-2-O-3517.
  9. Rodriguez-Sanz, H., Solis, M. T., Lopez, M. F., Gomez –Cadenas, A., Risueno, M. C. and Testillano, P. S. (2014). Involvement of auxin biosynthesis, action and transport in stress-induced microspore embryogenesis initiation and development. In: Pérez-Alfocea, F. and Acosta, M. (eds.). Fitohormonas: Metabolismo y Modo de Acción. Pictografía Ediciones. Murcia. CABI, 229-233.
  10. El-Tantawy, A. A., Solis, M. T., Da Costa, M. L., Coimbra, S., Risueno, M. C. and Testillano, P. S. (2013). Arabinogalactan protein profiles and distribution patterns during microspore embryogenesis and pollen development in Brassica napus. Plant Reprod 26(3): 231-243.
  11. Testillano, P. S., Solis, M. T. and Risueno, M. C. (2013). The 5-methyl-deoxy-cytidine (5mdC) localization to reveal in situ the dynamics of DNA methylation chromatin pattern in a variety of plant organ and tissue cells during development. Physiol Plant 149(1): 104-113.
  12. Solis, M. T., Rodriguez-Serrano, M., Meijon, M., Canal, M. J., Cifuentes, A., Risueno, M. C. and Testillano, P. S. (2012). DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis. J Exp Bot 63(18): 6431-6444.
  13. Prem, D., Solis, M. T., Barany, I., Rodriguez-Sanz, H., Risueno, M. C. and Testillano, P. S. (2012). A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12: 127.
  14. Rodriguez-Serrano, M., Barany, I., Prem, D., Coronado, M. J., Risueno, M. C. and Testillano, P. S. (2012). NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley. J Exp Bot 63(5): 2007-2024.
  15. Risueno, M. C. and Testillano, P. S. (2012). Nuclear reprogramming of plant differentiating cells: remodelling of nuclear domains and epigenetic regulation. In: Becerra, J., Santos-Ruíz, L. (eds). Hot topics in cell biology. Biohealthcare Publishing Limited, 71-74.
  16. Testillano, P. S., Rodriguez, M. D. (2012). Cell biology of plant development and adaptation, In: Becerra J, Santos-Ruíz L (eds). Hot topics in cell biology. Biohealthcare Publishing Limited, 61-62.
  17. Perez-Tienda, J., Testillano, P. S., Balestrini, R., Fiorilli, V., Azcon-Aguilar, C. and Ferrol, N. (2011). GintAMT2, a new member of the ammonium transporter family in the arbuscular mycorrhizal fungus Glomus intraradices. Fungal Genet Biol 48(11): 1044-1055.
  18. Bahaji, A., Ovecka, M., Barany, I., Risueno, M. C., Munoz, F. J., Baroja-Fernandez, E., Montero, M., Li, J., Hidalgo, M., Sesma, M. T., Ezquer, I., Testillano, P. S. and Pozueta-Romero, J. (2011). Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family. Plant Cell Physiol 52(4): 597-609.
  19. Segui-Simarro, J. M., Corral-Martinez, P., Corredor, E., Raska, I., Testillano, P. S. and Risueno, M. C. (2011). A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L. J Plant Physiol 168(8): 746-757.
  20. Germana, M. A., Chiancone, B., Padoan, D., Barany, I., Risueno, M. C.,Testillano, P. S. (2011). First stages of microspore reprogramming to embryogenesis through anther culture in Prunus armeniaca L. Env Exp Bot 71:152-157.
  21. Corredor, E., Risueno, M. C. and Testillano, P. S. (2010). Carbon-iron magnetic nanoparticles for agronomic use in plants: promising but still a long way to go. Plant Signal Behav 5(10): 1295-1297.
  22. Barany, I., Fadon, B., Risueno, M. C. and Testillano, P. S. (2010). Microspore reprogramming to embryogenesis induces changes in cell wall and starch accumulation dynamics associated with proliferation and differentiation events. Plant Signal Behav 5(4): 341-345
  23. Barany, I., Fadon, B., Risueno, M. C. and Testillano, P. S. (2010). Cell wall components and pectin esterification levels as markers of proliferation and differentiation events during pollen development and pollen embryogenesis in Capsicum annuum L. J Exp Bot 61(4): 1159-1175.
  24. Germana, M. A., Chiancone, B., Padoan, D., Barany, I., Risueno, M. C.,Testillano, P. S. (2010). Induction of pollen embryogenesis in seven cultivars of Prunus armeniaca l. Acta Hort 862: 273-278.
  25. Testillano, P. S., Coronado, M. J., Thierry, A. M., Matthys-Rochon, E., Risueno, M. C., (2010). In situ detection of Esr protein secretion during maize microspore embryogenesis and their secretion blockage show effects on the culture progression. Funct Plant Biol 37: 1-10.
  26. Prdao, M. J., Grueiro, M. P., Gonzalez, M. V., Testillano, P. S., Dominguez, C.,Lopez, M., REY M. (2010). Efficient plant regeneration throughsomatic embryogenesisfrom anthers and ovaries of six autochthonous grapevine cultivars from Galicia (Spain). Sci Hortic 125: 342-352.
  27. Zarrouk, O., Testillano, P. S., Risueno, M. C., Moreno, M. A., Gogorcena, Y. (2010). Changes in cell/tissue organization and peroxidase activity as markers for early detection of graft incompatibility in peach/plum combinations. J Am Soc Hortic Sci 135: 9-17.
  28. Risueno, M. C. and Testillano, P. S. (2009). Tracking gene and protein expression during microspore embryogenesis by Confocal Laser Scanning Microscopy. In: Touraev, A., Forster, B. P. and Mohan Jain, S. (eds). Advances in haploid production in higher plants. Springer Science and Bussines Media B.V., 339-347.
  29. Solis, M. T., Pintos B., Prado, M. J., Bueno, M. A., Risueno, M. C. and Testillano, P. S. (2006). Defined cellular changes as markers of microspore reprogramming to embryogenesis in Olea europaea L. In: Caruso, T. (ed). Biotecnology and quality of olive tree products around the mediterranean basin. Univ. Palermo Publishing. Marsala, 231-235.
  30. Perez-De-Luque, A., Lozano, M. D., Moreno, M. T., Testillano, P. S. and Rubiales, D. (2006). Pre-haustorial resistance against Orobanche crenata in faba bean. Proc. International Workshop on faba bean breeding and agronomy. Avila CM et al. (eds). Servicio de Publicaciones de la Viceconsejería de Agricultura de la Junta de Andalucía. Córdoba, 137-140.
  31. Barany, I., Gonzalez-Mwlendi, P., Coronado, M. J., Ramirez, C., Risueno, M. C. and Testillano, P. S. (2006). Plant cell wall components rearrange during cell reprogramming to embriogénesis. In: Iijima, S. (ed). Electron microscopy 2006. Microscopy Society of Japan, 245.
Protocols by Pilar Testillano
  1. TUNEL Assay in Kiwifruit Stigmatic Arms