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Romé Voulhoux


Ph.D. in Microbiology, CNRS, Aix Marseille University, France, 2000

Current position

CNRS Researcher & Group Leader at LISM CNRS Aix Marseille University, France


  1. Cadoret, F., Ball, G., Douzi, B. and Voulhoux, R.* (2014). Txc, a new type II secretion system of Pseudomonas aeruginosa strain PA7, is regulated by the TtsS/TtsR two-component system and directs specific secretion of the CbpE chitin-binding protein. J Bacteriol 196(13): 2376-2386.
  2. Bleves, S.*, Sana, T. G. and Voulhoux, R. (2014). The target cell genus does not matter. Trends Microbiol 22(6): 304-306.
  3. Bleves, S., Dunger, I., Walter, M. C., Frangoulidis, D., Kastenmüller, G., Voulhoux, R.* and Ruepp, A.* (2014). HoPaCI-DB: host-Pseudomonas and Coxiella interaction database. Nucleic Acids Res 42(D1): D671-D676.
  4. Arts, I. S., Ball, G., Leverrier, P., Garvis, S., Nicolaes, V., Vertommen, D., Ize, B., Dufe, V. T., Messens, J. and Voulhoux, R.* (2013). Dissecting the machinery that introduces disulfide bonds in Pseudomonas aeruginosa. MBio 4(6): e00912-00913
  5. Douzi, B., Filloux, A. and Voulhoux, R.* (2012). On the path to uncover the bacterial type II secretion system. Philos Trans R Soc Lond B Biol Sci 367(1592): 1059-1072.
  6. Douzi, B., Ball, G., Cambillau, C., Tegoni, M.* and Voulhoux, R.* (2011). Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates. J Biol Chem 286(47): 40792-40801.
  7. Durand, E., Alphonse, S., Brochier-Armanet, C., Ball, G., Douzi, B., Filloux, A., Bernard, C. and Voulhoux, R.* (2011). The assembly mode of the pseudopilus: a hallmark to distinguish a novel secretion system subtype. J Biol Chem 286(27): 24407-24416.
  8. Michel, G. P., Aguzzi, A., Ball, G., Soscia, C., Bleves, S. and Voulhoux, R.* (2011). Role of fimV in type II secretion system-dependent protein secretion of Pseudomonas aeruginosa on solid medium. Microbiology 157(Pt 7): 1945-1954.
  9. Franz, L. P., Douzi, B., Durand, E., Dyer, D. H., Voulhoux, R.* and Forest, K. T.* (2011). Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system. Acta Crystallogr D Biol Crystallogr 67(Pt 2): 124-130.
  10. Bleves, S., Viarre, V., Salacha, R., Michel, G. P., Filloux, A.* and Voulhoux, R.* (2010). Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol 300(8): 534-543.
  11. Salacha, R., Kovacic, F., Brochier-Armanet, C., Wilhelm, S., Tommassen, J., Filloux, A., Voulhoux, R. and Bleves, S. (2010). The Pseudomonas aeruginosa patatin-like protein PlpD is the archetype of a novel Type V secretion system. Environ Microbiol 12(6): 1498-1512.
  12. Alphonse, S., Durand, E., Douzi, B., Waegele, B., Darbon, H., Filloux, A., Voulhoux, R.* and Bernard, C.* (2010). Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the type II secretion system. J Struct Biol 169(1): 75-80.
  13. Douzi, B., Durand, E., Bernard, C., Alphonse, S., Cambillau, C., Filloux, A., Tegoni, M. and Voulhoux, R.* (2009). The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus. J Biol Chem 284(50): 34580-34589.
  14. Viarre, V., Cascales, E., Ball, G., Michel, G. P., Filloux, A. and Voulhoux, R.* (2009). HxcQ liposecretin is self-piloted to the outer membrane by its N-terminal lipid anchor. J Biol Chem 284(49): 33815-33823.
  15. Fox, A., Haas, D., Reimmann, C., Heeb, S., Filloux, A. and Voulhoux, R.* (2008). Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene. Appl Environ Microbiol 74(6): 1902-1908.
  16. Voulhoux, R.*, Filloux, A. and Schalk, I. J.* (2006). Pyoverdine-mediated iron uptake in Pseudomonas aeruginosa: the Tat system is required for PvdN but not for FpvA transport. J Bacteriol 188(9): 3317-3323.
  17. Voulhoux, R., Bos, M. P., Geurtsen, J., Mols, M. and Tommassen, J.* (2003). Role of a highly conserved bacterial protein in outer membrane protein assembly. Science 299(5604): 262-265.
  18. Voulhoux, R., Ball, G., Ize, B., Vasil, M. L., Lazdunski, A., Wu, L. F. and Filloux, A.* (2001). Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. EMBO J 20(23): 6735-6741.
  19. Voulhoux, R., Lazdunski, A. and Filloux, A.* (2001). Colicin A hybrids: a genetic tool for selection of type II secretion-proficient Pseudomonas strains. EMBO Rep 2(1): 49-54.
  20. Voulhoux, R., Taupiac, M. P., Czjzek, M., Beaumelle, B. and Filloux, A.* (2000). Influence of deletions within domain II of exotoxin A on its extracellular secretion from Pseudomonas aeruginosa. J Bacteriol 182(14): 4051-4058.

Book chapters

  1. Cadoret, F., Soscia, C. and Voulhoux, R.* (2014). Gene transfer: transformation/electroporation. Methods Mol Biol 1149: 11-15.
  2. Ize, B.*, Viarre, V. and Voulhoux, R.* (2014). Cell fractionation. Methods Mol Biol 1149: 185-191.
  3. Ball, G.*, Filloux, A. and Voulhoux, R. (2014). A method to capture large DNA fragments from genomic DNA. Methods Mol Biol 1149: 491-500.
  4. Michel, G. P. F.* and Voulhoux, R. (2008). The type II secretory system (T2SS) in Gram negative bacteria: a molecular nanomachine for secretion of Sec and Tat-dependent extracellular proteins. In: Wooldridge, K. (ed). Bacterial protein secretion systems. Horizon Scientific Press, p65-90.
  5. Tommassen, J. and Voulhoux, R. (2003). Biogenesis of outer membrane proteins. In: Oudega, B. (ed). Protein secretion pathways in bacteria. Kluwer Academic Publishers, 83-97.
Protocols by Romé Voulhoux
  1. In vitro Chitin Binding Assay