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Roberto De Michele


Ph.D. in Biotechnology, Department of Biology, University of Padova, Italy, 2005

Current position

Researcher at the National Research Council of Italy (CNR), Institute of Biosciences and Bioresources (IBBR), Palermo, Italy (2010-present)


  1. Ruberti, C., Barizza, E., Bodner, M., La Rocca, N., De Michele, R., Carimi, F., Lo Schiavo, F. and Zottini, M. (2014). Mitochondria change dynamics and morphology during grapevine leaf senescence. PLoS One 9(7): e102012.
  2. De Michele, R., Carimi, F. and Frommer, W. B. (2014). Mitochondrial biosensors. Int J Biochem Cell Biol 48: 39-44.
  3. Garfì, G., Mercati, F., Fontana, I., Collesano, G., Pasta, S., Vendramin, G. G., De Michele, R. and Carimi, F. (2013). Habitat features and genetic integrity of wild grapevine Vitis vinifera L. subsp. sylvestris (CC Gmel.) Hegi populations: A case study from Sicily. Flora-Morphology, Distribution, Functional Ecology of Plants 208(8): 538-548.
  4. Wang, Q., Zhao, Y., Luo, W., Li, R., He, Q., Fang, X., De Michele, R., Ast, C., von Wirén, N. and Lin, J. (2013). Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1; 3 by clustering and internalization. P Nat Acad Sci 110(32): 13204-13209.
  5. De Michele, R., Ast, C., Loque, D., Ho, C. H., Andrade, S. L., Lanquar, V., Grossmann, G., Gehne, S., Kumke, M. U. and Frommer, W. B. (2013). Fluorescent sensors reporting the activity of ammonium transceptors in live cells. Elife 2: e00800.
  6. De Michele, R., Loqué, D., Lalonde, S. and Frommer, W. B. (2012). Ammonium and urea transporter inventory of the Selaginella and Physcomitrella genomes. Front Plant Sci 3.
  7. Espunya, M. C., De Michele, R., Gomez-Cadenas, A. and Martinez, M. C. (2012). S-Nitrosoglutathione is a component of wound- and salicylic acid-induced systemic responses in Arabidopsis thaliana. J Exp Bot 63(8): 3219-3227.
  8. Marquès-Bueno, M. M., Moreno-Romero, J., Abas, L., De Michele, R. and Martínez, M. C. (2011). Linking protein kinase CK2 and auxin transport. Plant Sign Behav 6(10): 1603-1605.
  9. Marques-Bueno, M. M., Moreno-Romero, J., Abas, L., De Michele, R. and Martinez, M. C. (2011). A dominant negative mutant of protein kinase CK2 exhibits altered auxin responses in Arabidopsis. Plant J 67(1): 169-180.
  10. Carimi, F., Mercati, F., De Michele, R., Fiore, M. C., Riccardi, P. and Sunseri, F. (2011). Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’as revealed by microsatellite markers. Genet Resour Crop Ev 58(7): 967-975.
  11. Zottini, M., Costa, A., De, M. R., Lo, S. F. (2010). Role of nitric oxide in programmed cell death. In: Capitolo in Nitric Oxide in Plant Physiology. WEINHEIM: WILEY-VCH VERLAG GMBH, 77-87,
  12. De Michele, R., Vurro, E., Rigo, C., Costa, A., Elviri, L., Di Valentin, M., Careri, M., Zottini, M., Sanita di Toppi, L. and Lo Schiavo, F. (2009). Nitric oxide is involved in cadmium-induced programmed cell death in Arabidopsis suspension cultures. Plant Physiol 150(1): 217-228.
  13. De Michele, R., Formentin, E., Todesco, M., Toppo, S., Carimi, F., Zottini, M., Barizza, E., Ferrarini, A., Delledonne, M., Fontana, P. and Lo Schiavo, F. (2009). Transcriptome analysis of Medicago truncatula leaf senescence: similarities and differences in metabolic and transcriptional regulations as compared with Arabidopsis, nodule senescence and nitric oxide signalling. New Phytol 181(3): 563-575.
  14. De Michele, R., Formentin, E. and Lo Schiavo, F. (2009). Legume leaf senescence: a transcriptional analysis. Plant Signal Behav 4(4): 319-320.
  15. Zottini, M., Costa, A., De Michele, R., Ruzzene, M., Carimi, F. and Lo Schiavo, F. (2007). Salicylic acid activates nitric oxide synthesis in Arabidopsis. J Exp Bot 58(6): 1397-1405.
  16. Libourel, I. G., Bethke, P. C., De Michele, R. and Jones, R. L. (2006). Nitric oxide gas stimulates germination of dormant Arabidopsis seeds: use of a flow-through apparatus for delivery of nitric oxide. Planta 223(4): 813-820.
  17. De, M. R., Zottini, M., Lo, S. F. and Carimi, F. (2006). Cross talk between nitric oxide and growth regulators. In: Floricolture, Ornamental and Plant Biotechnology. Global Science Books, vol 3.
  18. Siragusa, M., De, M. R., Carra, A. and Carimi, F. (2006). Aspetti cellulari, fisiologici e molecolari della senescenza nelle piante. Italus Hortus 13 (4): 74-83.
  19. Carimi, F., Zottini, M., Costa, A., Cattelan, I., De Michele, R., Terzi, M. and Lo Schiavo, F. (2005). NO signalling in cytokinin‐induced programmed cell death. Plant, Cell & Environment 28(9): 1171-1178.
  20. Zottini, M., Terzi, M., De, M. R, Lo, S. F. and Carimi, F. (2005), NO signaling and cytokinins. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 141 (3): supplement S, S238.
  21. Carimi, F., Terzi, M., De Michele, R., Zottini, M. and Lo Schiavo, F. (2004). High levels of the cytokinin BAP induce PCD by accelerating senescence. Plant Science 166(4): 963-969.
Protocols by Roberto De Michele
  1. Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors