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Holden Maecker


Ph.D. in Cancer Biology, Stanford University, 1988

Current position

Investigator, Baylor Institute of Immunology Research, Dallas, TX (part-time consultant) (2008-present)
Director, Human Immune Monitoring Center, Stanford University, Stanford, CA (2009-present)
Associate Professor (Research), Microbiology & Immunology, Stanford University, Stanford, CA (2011-present)


Selected peer-reviewed publications

  1. DiCarlo, J., Agarwal-Hashmi, R., Shah, A., Kim, P., Craveiro, L., Killen, R., Rosenberg-Hasson, Y. and Maecker, H. (2014). Cytokine and chemokine patterns across 100 days after hematopoietic stem cell transplantation in children. Biol Blood Marrow Transplant 20(3): 361-369.
  2. Rosenberg-Hasson, Y., Hansmann, L., Liedtke, M., Herschmann, I. and Maecker, H. T. (2014). Effects of serum and plasma matrices on multiplex immunoassays. Immunol Res 58(2-3): 224-233.
  3. Chang, S., Kohrt, H. and Maecker, H. T. (2014). Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother 63(7): 713-719.
  4. Leipold, M. and Maecker, H. T. (2013). Multiparameter phenotyping of human PBMCs using mass cytometry. In: Shaw, A. (ed). Immunosenescence.
  5. Furman, D., Jojic, V., Kidd, B., Shen-Orr, S., Price, J., Jarrell, J., Tse, T., Huang, H., Lund, P., Maecker, H. T., Utz, P. J., Dekker, C. L., Koller, D. and Davis, M. M. (2013). Apoptosis and other immune biomarkers predict influenza vaccine responsiveness. Mol Syst Biol 9: 659.
  6. Horowitz, A., Strauss-Albee, D. M., Leipold, M., Kubo, J., Nemat-Gorgani, N., Dogan, O. C., Dekker, C. L., Mackey, S., Maecker, H., Swan, G. E., Davis, M. M., Norman, P. J., Guethlein, L. A., Desai, M., Parham, P. and Blish, C. A. (2013). Genetic and environmental determinants of human NK cell diversity revealed by mass cytometry. Sci Transl Med 5(208): 208ra145.
  7. Britten, C. M., Janetzki, S., Butterfield, L. H., Ferrari, G., Gouttefangeas, C., Huber, C., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., O'Donnell-Tormey, J., Odunsi, K., Old, L. J., Ottenhoff, T. H., Ottensmeier, C., Pawelec, G., Roederer, M., Roep, B. O., Romero, P., van der Burg, S. H., Walter, S., Hoos, A. and Davis, M. M. (2012). T cell assays and MIATA: the essential minimum for maximum impact. Immunity 37(1): 1-2.
  8. Leipold, M. D. and Maecker, H. T. (2012). Mass cytometry: protocol for daily tuning and running cell samples on a CyTOF mass cytometer. J Vis Exp(69): e4398.
  9. Maecker, H. T., McCoy, J. P. and Nussenblatt, R. (2012). Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol 12(3): 191-200.
  10. Siebert, J. C., Munsil, W., Rosenberg-Hasson, Y., Davis, M. M. and Maecker, H. T. (2012). The Stanford Data Miner: a novel approach for integrating and exploring heterogeneous immunological data. J Transl Med 10: 62.
  11. Streitz, M., Fuhrmann, S., Thomas, D., Cheek, E., Nomura, L., Maecker, H., Martus, P., Aghaeepour, N., Brinkman, R. R., Volk, H. D. and Kern, F. (2012). The phenotypic distribution and functional profile of tuberculin-specific CD4 T-cells characterizes different stages of TB infection. Cytometry B Clin Cytom 82(6): 360-368.
  12. Streitz, M., Fuhrmann, S., Powell, F., Quassem, A., Nomura, L., Maecker, H., Martus, P., Volk, H. D. and Kern, F. (2011). Tuberculin-specific T cells are reduced in active pulmonary tuberculosis compared to LTBI or status post BCG vaccination. J Infect Dis 203(3): 378-382.
  13. Jaimes, M. C., Maecker, H. T., Yan, M., Maino, V. C., Hanley, M. B., Greer, A., Darden, J. M. and D'Souza, M. P. (2011). Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testing. J Immunol Methods 363(2): 143-157.
  14. Maecker, H. T., McCoy, J. P., Jr., Consortium, F. H. I., Amos, M., Elliott, J., Gaigalas, A., Wang, L., Aranda, R., Banchereau, J., Boshoff, C., Braun, J., Korin, Y., Reed, E., Cho, J., Hafler, D., Davis, M., Fathman, C. G., Robinson, W., Denny, T., Weinhold, K., Desai, B., Diamond, B., Gregersen, P., Di Meglio, P., Nestle, F. O., Peakman, M., Villanova, F., Ferbas, J., Field, E., Kantor, A., Kawabata, T., Komocsar, W., Lotze, M., Nepom, J., Ochs, H., O'Lone, R., Phippard, D., Plevy, S., Rich, S., Roederer, M., Rotrosen, D. and Yeh, J. H. (2010). A model for harmonizing flow cytometry in clinical trials. Nat Immunol 11(11): 975-978.
  15. Janetzki, S., Britten, C. M., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., Old, L. J., Romero, P., Hoos, A. and Davis, M. M. (2009). "MIATA"-minimal information about T cell assays. Immunity 31(4): 527-528.
Protocols by Holden Maecker
  1. Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
  2. Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
  3. Intracellular Cytokine Staining (ICS) on Human Lymphocytes or Peripheral Blood Mononuclear Cells (PBMCs)
  4. Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry
  5. Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry
  6. [Bio101] Cryopreservation of Human Serum