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Darrell N. Kotton


M. D. in Washington University School of Medicine, 1994

Current position

Associate Professor of Medicine and Associate Professor of Pathology and Laboratory Medicine, Boston University School of Medicine
Director, Center for Regenerative Medicine, Boston University and Boston Medical Center
Assistant Director, In vivo imaging Core (IVIS), BUMC.
Attending Physician, Pulmonary and Critical Care Medicine, Boston Medical Center, Boston

Publications (since 2005)

  1. Kotton, D. N. (2012). Next-generation regeneration: the hope and hype of lung stem cell research. Am J Respir Crit Care Med 185(12): 1255-1260.
  2. Longmire, T. A., Ikonomou, L., Hawkins, F., Christodoulou, C., Cao, Y., Jean, J. C., Kwok, L. W., Mou, H., Rajagopal, J., Shen, S. S., Dowton, A. A., Serra, M., Weiss, D. J., Green, M. D., Snoeck, H. W., Ramirez, M. I. and Kotton, D. N. (2012). Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells. Cell Stem Cell 10(4): 398-411.
  3. Cheng, X., Ying, L., Lu, L., Galvao, A. M., Mills, J. A., Lin, H. C., Kotton, D. N., Shen, S. S., Nostro, M. C., Choi, J. K., Weiss, M. J., French, D. L. and Gadue, P. (2012). Self-renewing endodermal progenitor lines generated from human pluripotent stem cells. Cell Stem Cell 10(4): 371-384.
  4. Ohle, S. J., Anandaiah, A., Fabian, A. J., Fine, A. and Kotton, D. N. (2012). Maintenance and repair of the lung endothelium does not involve contributions from marrow-derived endothelial precursor cells. Am J Respir Cell Mol Biol 47(1): 11-19.
  5. Kotton, D. N., Muse, V. V. and Nishino, M. (2012). Case records of the Massachusetts General Hospital. Case 2-2012. A 63-year-old woman with dyspnea and rapidly progressive respiratory failure. N Engl J Med 366(3): 259-269.
  6. Christodoulou, C. and Kotton, D. N. (2012). Are embryonic stem and induced pluripotent stem cells the same or different? Implications for their potential therapeutic use. Cell Cycle 11(1): 5-6.
  7. Bais, M. V., Shabin, Z. M., Young, M., Einhorn, T. A., Kotton, D. N. and Gerstnefeld, L. C. (2012). Role of Nanog in the maintenance of marrow stromal stem cells during post natal bone regeneration. Biochem Biophys Res Commun 417(1): 211-216.
  8. Wang, K., Terrenoire, C., Sampson, K. J., Iyer, V., Osteen, J. D., Lu, J., Keller, G., Kotton, D. N. and Kass, R. S. (2011). Biophysical properties of slow potassium channels in human embryonic stem cell derived cardiomyocytes implicate subunit stoichiometry. J Physiol 589(Pt 24): 6093-6104.
  9. Ikonomou, L., Hemnes, A. R., Bilousova, G., Hamid, R., Loyd, J. E., Hatzopoulos, A. K., Kotton, D. N., Majka, S. M. and Austin, E. D. (2011). Programmatic change: lung disease research in the era of induced pluripotency. Am J Physiol Lung Cell Mol Physiol 301(6): L830-835.
  10. Studwell, A. J. and Kotton, D. N. (2011). A shift from cell cultures to creatures: in vivo imaging of small animals in experimental regenerative medicine. Mol Ther 19(11): 1933-1941.
  11. Borok, Z., Whitsett, J. A., Bitterman, P. B., Thannickal, V. J., Kotton, D. N., Reynolds, S. D., Krasnow, M. A., Bianchi, D. W., Morrisey, E. E., Hogan, B. L., Kurie, J. M., Walker, D. C., Radisky, D. C., Nishimura, S. L., Violette, S. M., Noble, P. W., Shapiro, S. D., Blaisdell, C. J., Chapman, H. A., Kiley, J., Gail, D. and Hoshizaki, D. (2011). Cell plasticity in lung injury and repair: report from an NHLBI workshop, April 19-20, 2010. Proc Am Thorac Soc 8(3): 215-222.
  12. Christodoulou, C., Longmire, T. A., Shen, S. S., Bourdon, A., Sommer, C. A., Gadue, P., Spira, A., Gouon-Evans, V., Murphy, G. J., Mostoslavsky, G. and Kotton, D. N. (2011). Mouse ES and iPS cells can form similar definitive endoderm despite differences in imprinted genes. J Clin Invest 121(6): 2313-2325.
  13. Sahin, E., Colla, S., Liesa, M., Moslehi, J., Muller, F. L., Guo, M., Cooper, M., Kotton, D., Fabian, A. J., Walkey, C., Maser, R. S., Tonon, G., Foerster, F., Xiong, R., Wang, Y. A., Shukla, S. A., Jaskelioff, M., Martin, E. S., Heffernan, T. P., Protopopov, A., Ivanova, E., Mahoney, J. E., Kost-Alimova, M., Perry, S. R., Bronson, R., Liao, R., Mulligan, R., Shirihai, O. S., Chin, L. and DePinho, R. A. (2011). Telomere dysfunction induces metabolic and mitochondrial compromise. Nature 470(7334): 359-365.
  14. Siqueira, M. F., Flowers, S., Bhattacharya, R., Faibish, D., Behl, Y., Kotton, D. N., Gerstenfeld, L., Moran, E. and Graves, D. T. (2011). FOXO1 modulates osteoblast differentiation. Bone 48(5): 1043-1051. [Epub ahead of print].
  15. Novak, A., Shtrichman, R., Germanguz, I., Segev, H., Zeevi-Levin, N., Fishman, B., Mandel, Y. E., Barad, L., Domev, H., Kotton, D., Mostoslavsky, G., Binah, O. and Itskovitz-Eldor, J. (2010). Enhanced reprogramming and cardiac differentiation of human keratinocytes derived from plucked hair follicles, using a single excisable lentivirus. Cell Reprogram 12(6): 665-678.
  16. Somers, A., Jean, J. C., Sommer, C. A., Omari, A., Ford, C. C., Mills, J. A., Ying, L., Sommer, A. G., Jean, J. M., Smith, B. W., Lafyatis, R., Demierre, M. F., Weiss, D. J., French, D. L., Gadue, P., Murphy, G. J., Mostoslavsky, G. and Kotton, D. N. (2010). Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette. Stem Cells 28(10): 1728-1740.
  17. Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D. and Vacanti, J. P. (2010). Regeneration and orthotopic transplantation of a bioartificial lung. Nat Med 16(8): 927-933.
  18. Wilson, A. A., Murphy, G. J., Hamakawa, H., Kwok, L. W., Srinivasan, S., Hovav, A. H., Mulligan, R. C., Amar, S., Suki, B. and Kotton, D. N. (2010). Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. J Clin Invest 120(1): 379-389.
  19. Ford C.C. and Kotton DN. Chapter 27: “Human Pluripotent Cells for Regenerative Medicine”. Text book: Human Stem Cell Technology and Biology: Research Guide and Laboratory Manual. John Wiley and Sons, Inc. in press. (2010).
  20. Sommer, C. A., Sommer, A. G., Longmire, T. A., Christodoulou, C., Thomas, D. D., Gostissa, M., Alt, F. W., Murphy, G. J., Kotton, D. N. and Mostoslavsky, G. (2010). Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector. Stem Cells 28(1): 64-74.
  21. Cao, Y., Vo, T., Millien, G., Tagne, J. B., Kotton, D., Mason, R. J., Williams, M. C. and Ramirez, M. I. (2010). Epigenetic mechanisms modulate thyroid transcription factor 1-mediated transcription of the surfactant protein B gene. J Biol Chem 285(3): 2152-2164.
  22. Wu, J., Qian, J., Li, C., Kwok, L., Cheng, F., Liu, P., Perdomo, C., Kotton, D., Vaziri, C., Anderlind, C., Spira, A., Cardoso, W. V. and Lu, J. (2010). miR-129 regulates cell proliferation by downregulating Cdk6 expression. Cell Cycle 9(9): 1809-1818.
  23. Furmanov, K., Elnekave, M., Lehmann, D., Clausen, B. E., Kotton, D. N. and Hovav, A. H. (2010). The role of skin-derived dendritic cells in CD8+ T cell priming following immunization with lentivectors. J Immunol 184(9): 4889-4897.
  24. Greenhill, S. R. and Kotton, D. N. (2009). Pulmonary alveolar proteinosis: a bench-to-bedside story of granulocyte-macrophage colony-stimulating factor dysfunction. Chest 136(2): 571-577.
  25. Bais, M., McLean, J., Sebastiani, P., Young, M., Wigner, N., Smith, T., Kotton, D. N., Einhorn, T. A. and Gerstenfeld, L. C. (2009). Transcriptional analysis of fracture healing and the induction of embryonic stem cell-related genes. PLoS One 4(5): e5393.
  26. Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G. “iPS Cell Generation Using a Single Lentiviral Stem Cell Cassette.” Stem Cells. Dec 18, 2008 [Epub ahead of print].
  27. Cardoso WV, and Kotton DN. “Specification and Patterning of the Respiratory System.” StemBook. 2008.
  28. Wilson, A. A., Kwok, L. W., Hovav, A. H., Ohle, S. J., Little, F. F., Fine, A. and Kotton, D. N. (2008). Sustained expression of alpha1-antitrypsin after transplantation of manipulated hematopoietic stem cells. Am J Respir Cell Mol Biol 39(2): 133-141.
  29. Wilson, A. A. and Kotton, D. N. (2008). Another notch in stem cell biology: Drosophila intestinal stem cells and the specification of cell fates. Bioessays 30(2): 107-109.
  30. Murphy, J., Summer, R., Wilson, A. A., Kotton, D. N. and Fine, A. (2008). The prolonged life-span of alveolar macrophages. Am J Respir Cell Mol Biol 38(4): 380-385.
  31. Pan, H., Mostoslavsky, G., Eruslanov, E., Kotton, D. N. and Kramnik, I. (2008). Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages. J Immunol Methods 329(1-2): 31-44.
  32. Kotton, D. N. and Fine, A. (2008). Lung stem cells. Cell Tissue Res 331(1): 145-156.
  33. Murphy, G. J., Mostoslavsky, G., Kotton, D. N. and Mulligan, R. C. (2006). Exogenous control of mammalian gene expression via modulation of translational termination. Nat Med 12(9): 1093-1099.
  34. Kotton, D. N., Fabian, A. J. and Mulligan, R. C. (2005). Failure of bone marrow to reconstitute lung epithelium. Am J Respir Cell Mol Biol 33(4): 328-334.
  35. Kotton, D. N., Fabian, A. J. and Mulligan, R. C. (2005). A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity. Blood 106(5): 1574-1580.
  36. Mostoslavsky, G., Kotton, D. N., Fabian, A. J., Gray, J. T., Lee, J. S. and Mulligan, R. C. (2005). Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulation. Mol Ther 11(6): 932-940.
  37. Summer, R., Kotton, D. N., Liang, S., Fitzsimmons, K., Sun, X. and Fine, A. (2005). Embryonic lung side population cells are hematopoietic and vascular precursors. Am J Respir Cell Mol Biol 33(1): 32-40.
Protocols by Darrell N. Kotton
  1. Mouse ESC Differentiation to Nkx2.1+ Lung and Thyroid Progenitors