Education

DM, Postgraduate Institute of Medical Education and Research, Chandigarh, 2017

Lab information

Lab Name: Pulkit Rastogi Laboratory
Institution: Department of Hematology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India
Lab Head: Dr. Pulkit Rastogi, MD, DM (Hematopathology)
Designation: Associate Professor, Hematology
Research Focus:
Our laboratory specializes in molecular diagnostics and translational research in hematological malignancies, including acute leukemias, myelodysplastic syndromes, myeloproliferative neoplasms, and inherited hematologic conditions. We focus on the integration of advanced molecular techniques such as RT-PCR, digital PCR, NGS, and fibroblast culture protocols for germline variant assessment.

Key Techniques:

Molecular diagnostics (RT-PCR, ddPCR, Sanger, NGS)

Flow cytometry and immunophenotyping

Cytogenetics and FISH

Skin fibroblast culture for germline studies

Hematology automation and informatics

Recent Projects:

Germline-somatic interface in AML/MDS

Utility of PCR-based clonality assays in T-cell lymphoproliferative disorders

Fibroblast culture optimization for downstream genetic analysis
https://pgihematology.com/

Research focus

The Pulkit Rastogi Lab focuses on the molecular and translational aspects of hematologic malignancies, with a particular emphasis on acute leukemia, MDS, and myeloproliferative neoplasms. Our work integrates advanced molecular techniques—including RT-PCR, digital PCR, and next-generation sequencing—to improve diagnostic accuracy and explore genotype–phenotype correlations. We are also actively involved in germline variant assessment through cultured skin fibroblasts, with applications in hereditary predisposition syndromes. Additional interests include assay development for T-cell clonality, quality assurance in hematopathology, and lab automation for streamlined molecular reporting.

Publications