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Andrea   Puhar

Andrea Puhar

Education

Ph.D, Programme “Molecular and Cellular Biology and Pathology”, University of Padua, Italy

Current position

Group Leader, The Laboratory for Molecular Infection Medicine Sweden (MIMS)-The Nordic EMBL Partnership for Molecular Medicine and Department of Molecular Biology, Umeå University, Sweden

Publications

Peer-reviewed publications

  1. Boal, F., Puhar, A., Xuereb, J. M., Kunduzova, O., Sansonetti, P. J., Payrastre, B. and Tronchere, H. (2016). PI5P triggers ICAM-1 degradation in shigella infected cells, thus dampening immune cell recruitment. Cell Rep 14(4): 750-759.
  2. Bravo, V., Puhar, A., Sansonetti, P., Parsot, C. and Toro, C. S. (2015). Distinct mutations led to inactivation of type 1 fimbriae expression in Shigella spp. PLoS One 10(3): e0121785.
  3. Puhar, A. and Sansonetti, P. J. (2014). Type III secretion system. Curr Biol 24(17): R784-791.
  4. Puhar, A. and Sansonetti, P. J. (2014). Induction of connexin-hemichannel opening. Bio-protocol 4(17): e1220. (Corresponding author) Featured protocol
  5. Puhar, A. and Sansonetti, P. J. (2014). Dye-uptake experiment through connexin hemichannels. Bio-protocol 4(17): e1221. (Corresponding author) Featured protocol
  6. Puhar, A., Tronchere, H., Payrastre, B., Nhieu, G. T. and Sansonetti, P. J. (2013). A Shigella effector dampens inflammation by regulating epithelial release of danger signal ATP through production of the lipid mediator PtdIns5P. Immunity 39(6): 1121-1131.
  7. Teo, I., Toms, S. M., Marteyn, B., Barata, T. S., Simpson, P., Johnston, K. A., Schnupf, P., Puhar, A., Bell, T., Tang, C., Zloh, M., Matthews, S., Rendle, P. M., Sansonetti, P. J. and Shaunak, S. (2012). Preventing acute gut wall damage in infectious diarrhoeas with glycosylated dendrimers. EMBO Mol Med 4(9): 866-881.
  8. Tran, S. L., Puhar, A., Ngo-Camus, M. and Ramarao, N. (2011). Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. PLoS One 6(9): e22876.
  9. Konradt, C., Frigimelica, E., Nothelfer, K., Puhar, A., Salgado-Pabon, W., di Bartolo, V., Scott-Algara, D., Rodrigues, C. D., Sansonetti, P. J. and Phalipon, A. (2011). The Shigella flexneri type three secretion system effector IpgD inhibits T cell migration by manipulating host phosphoinositide metabolism. Cell Host Microbe 9(4): 263-272.
  10. Tran, S. L., Guillemet, E., Ngo-Camus, M., Clybouw, C., Puhar, A., Moris, A., Gohar, M., Lereclus, D. and Ramarao, N. (2011). Haemolysin II is a Bacillus cereus virulence factor that induces apoptosis of macrophages. Cell Microbiol 13(1): 92-108.
  11. Puhar, A., Dal Molin, F., Horvath, S., Ladant, D. and Montecucco, C. (2008). Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases. PLoS One 3(10): e3564.
  12. Dal Molin, F., Zornetta, I., Puhar, A., Tonello, F., Zaccolo, M. and Montecucco, C. (2008). cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin. Biochem Biophys Res Commun 376(2): 429-433.
  13. Lin, P. C., Puhar, A. and Steuber, J. (2008). NADH oxidation drives respiratory Na+ transport in mitochondria from Yarrowia lipolytica. Arch Microbiol 190(4): 471-480.
  14. Puhar, A. and Montecucco, C. (2007). Where and how do anthrax toxins exit endosomes to intoxicate host cells? Trends Microbiol 15(11): 477-482. (Corresponding author)
  15. Genisset, C.*, Puhar, A.*, Calore, F., de Bernard, M., Dell'Antone, P. and Montecucco, C. (2007). The concerted action of the Helicobacter pylori cytotoxin VacA and of the v-ATPase proton pump induces swelling of isolated endosomes. Cell Microbiol 9(6): 1481-1490.
  16. Lin, P. C.*, Puhar, A.*, Turk, K., Piligkos, S., Bill, E., Neese, F. and Steuber, J. (2005). A vertebrate-type ferredoxin domain in the Na+-translocating NADH dehydrogenase from Vibrio cholerae. J Biol Chem 280(24): 22560-22563. (*Equal contribution)
  17. Puhar, A., Johnson, E. A., Rossetto, O. and Montecucco, C. (2004). Comparison of the pH-induced conformational change of different clostridial neurotoxins. Biochem Biophys Res Commun 319(1): 66-71.
  18. Turk, K., Puhar, A., Neese, F., Bill, E., Fritz, G. and Steuber, J. (2004). NADH oxidation by the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae: functional role of the NqrF subunit. J Biol Chem 279(20): 21349-21355.

Book chapters

  1. Sansonetti, P. J. and Puhar, A. (2010). Overview of bacterial pathogens. In: Kaufmann, S. H. E., Rouse, B. T. and Sacks, D. L. (eds). The Immune Response to Infection. ASM Press.
  2. Türk, K., Puhar, A., Dimroth, P. and Steuber, J. (2002). Spectroscopic Properties of NqrF, the Electron Input Subunit of the Na+-translocating NADH:Quinone Oxidoreductase: a FAD–Fe/S–Protein. In: Perham, S. C. R. and Scrutton, N. (eds). Flavins and Flavoproteins. Agency for Scientific Publication, 667- 672.
Protocols by Andrea Puhar
  1. Induction of Connexin-hemichannel Opening
  2. Dye-uptake Experiment through Connexin Hemichannels