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Jun Ishii

Education

Ph.D. in Engineering, Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, Japan, 2008

Current position

Associate Professor, Organization of Advanced Science and Technology, Kobe University, Japan

Publications

  1. Nishimura, Y., Ishii, J., Ogino, C. and Kondo, A. (2014). Genetic Engineering of Bio-Nanoparticles for Drug Delivery: A Review. Journal of Biomedical Nanotechnology 10(9): 2063-2085.
  2. Ishii, J., Kondo, T., Makino, H., Ogura, A., Matsuda, F. and Kondo, A. (2014). Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae. FEMS Yeast Res 14(3): 399-411.
  3. Nishimura, Y., Takeda, K., Ezawa, R., Ishii, J., Ogino, C. and Kondo, A. (2014). A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway. J Nanobiotechnology 12: 11.
  4. Ishii, J., Oda, A., Togawa, S., Fukao, A., Fujiwara, T., Ogino, C. and Kondo, A. (2014). Microbial fluorescence sensing for human neurotensin receptor type 1 using Galpha-engineered yeast cells. Anal Biochem 446: 37-43.
  5. Nakamura, Y., Takemoto, N., Ishii, J. and Kondo, A. (2014). Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system. Biotechnol Bioeng 111(3): 586-596.
  6. Matsuda, F., Ishii, J., Kondo, T., Ida, K., Tezuka, H. and Kondo, A. (2013). Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance. Microb Cell Fact 12: 119.
  7. Nakamura, Y., Ishii, J. and Kondo, A. (2013). Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells. PLoS One 8(12): e82237.
  8. Nishimura, Y., Mieda, H., Ishii, J., Ogino, C., Fujiwara, T. and Kondo, A. (2013). Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes. J Nanobiotechnology 11: 19.
  9. Matsuda, F., Shirai, T., Ishii, J. and Kondo, A. (2013). Regulation of central carbon metabolism in Saccharomyces cerevisiae by metabolic inhibitors. J Biosci Bioeng 116(1): 59-64.
  10. Nakamura, Y., Ishii, J. and Kondo, A. (2013). Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System. PLoS One 8(6): e66793.
  11. Hasunuma, T., Okazaki, F., Okai, N., Hara, K. Y., Ishii, J. and Kondo, A. (2013). A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology. Bioresour Technol 135: 513-522.
  12. Nishimura, Y., Takeda, K., Ishii, J., Ogino, C. and Kondo, A. (2013). An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules. J Virol Methods 189(2): 393-396.
  13. Nishimura, Y., Mimura, W., Mohamed Suffian, I. F., Amino, T., Ishii, J., Ogino, C. and Kondo, A. (2013). Granting specificity for breast cancer cells using a hepatitis B core particle with a HER2-targeted affibody molecule. J Biochem 153(3): 251-256.
  14. Kondo, A., Ishii, J., Hara, K. Y., Hasunuma, T. and Matsuda, F. (2013). Development of microbial cell factories for bio-refinery through synthetic bioengineering. J Biotechnol 163(2): 204-216.
  15. Ishii, J., Yoshimura, K., Hasunuma, T. and Kondo, A. (2013). Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway. Appl Microbiol Biotechnol 97(6): 2597-2607.
  16. Suga, H., Matsuda, F., Hasunuma, T., Ishii, J. and Kondo, A. (2013). Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 97(4): 1669-1678.
  17. Matsuda, F., Kondo, T., Ida, K., Tezuka, H., Ishii, J. and Kondo, A. (2012). Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of Saccharomyces cerevisiae. Biosci Biotechnol Biochem 76(11): 2139-2141.
  18. Nishimura, Y., Ishii, J., Okazaki, F., Ogino, C. and Kondo, A. (2012). Complex carriers of affibody-displaying bio-nanocapsules and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery. J Drug Target 20(10): 897-905.
  19. Fukutani, Y., Ishii, J., Noguchi, K., Kondo, A. and Yohda, M. (2012). An improved bioluminescence-based signaling assay for odor sensing with a yeast expressing a chimeric olfactory receptor. Biotechnol Bioeng 109(12): 3143-3151.
  20. Ishii, J., Yoshimoto, N., Tatematsu, K., Kuroda, S., Ogino, C., Fukuda, H. and Kondo, A. (2012). Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface. PLoS One 7(5): e37136.
  21. Ishii, J., Moriguchi, M., Hara, K. Y., Shibasaki, S., Fukuda, H. and Kondo, A. (2012). Improved identification of agonist-mediated Galpha(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry. Anal Biochem 426(2): 129-133.
  22. Hara, K. Y., Kim, S., Kiriyama, K., Yoshida, H., Arai, S., Ishii, J., Ogino, C., Fukuda, H. and Kondo, A. (2012). An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae. Biotechnol J 7(5): 686-689.
  23. Ryo, S., Ishii, J., Iguchi, Y., Fukuda, N. and Kondo, A. (2012). Transplantation of the GAL regulon into G-protein signaling circuitry in yeast. Anal Biochem 424(1): 27-31.
  24. Kondo, T., Tezuka, H., Ishii, J., Matsuda, F., Ogino, C. and Kondo, A. (2012). Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae. J Biotechnol 159(1-2): 32-37.
  25. Nishimura, Y., Shishido, T., Ishii, J., Tanaka, T., Ogino, C. and Kondo, A. (2012). Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences. J Biotechnol 157(1): 124-129.
  26. Fukutani, Y., Nakamura, T., Yorozu, M., Ishii, J., Kondo, A. and Yohda, M. (2012). The N-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor. Biotechnol Bioeng 109(1): 205-212.
  27. Matsuda, F., Furusawa, C., Kondo, T., Ishii, J., Shimizu, H. and Kondo, A. (2011). Engineering strategy of yeast metabolism for higher alcohol production. Microb Cell Fact 10: 70.
  28. Fukuda, N., Ishii, J. and Kondo, A. (2011). Ggamma recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions. FEBS J 278(17): 3086-3094.
  29. Fukuda, N., Ishii, J., Kaishima, M. and Kondo, A. (2011). Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast. Anal Biochem 417(2): 182-187.
  30. Hasunuma, T., Sanda, T., Yamada, R., Yoshimura, K., Ishii, J. and Kondo, A. (2011). Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Microb Cell Fact 10(1): 2.
  31. Tanino, T., Hotta, A., Ito, T., Ishii, J., Yamada, R., Hasunuma, T., Ogino, C., Ohmura, N., Ohshima, T. and Kondo, A. (2010). Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation. Appl Microbiol Biotechnol 88(5): 1215-1221.
  32. Iguchi, Y., Ishii, J., Nakayama, H., Ishikura, A., Izawa, K., Tanaka, T., Ogino, C. and Kondo, A. (2010). Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast. J Biochem 147(6): 875-884.
  33. Togawa, S., Ishii, J., Ishikura, A., Tanaka, T., Ogino, C. and Kondo, A. (2010). Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem 147(6): 867-873.
  34. Ishii, J., Fukuda, N., Tanaka, T., Ogino, C. and Kondo, A. (2010). Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling. FEBS J 277(9): 1982-1995.
  35. Fukuda, N., Ishii, J., Tanaka, T. and Kondo, A. (2010). The competitor-introduced Ggamma recruitment system, a new approach for screening affinity-enhanced proteins. FEBS J 277(7): 1704-1712.
  36. Tanaka, T., Masunari, S., Ishii, J., Wakamura, K., Segawa, M., Fukuda, H. and Kondo, A. (2010). Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction. J Biotechnol 145(1): 79-83.
  37. Ishii, J., Izawa, K., Matsumura, S., Wakamura, K., Tanino, T., Tanaka, T., Ogino, C., Fukuda, H. and Kondo, A. (2009). A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast. J Biochem 145(6): 701-708.
  38. Fukuda, N., Ishii, J., Tanaka, T., Fukuda, H. and Kondo, A. (2009). Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling. FEBS J 276(9): 2636-2644.
  39. Ishii, J., Tanaka, T., Matsumura, S., Tatematsu, K., Kuroda, S., Ogino, C., Fukuda, H. and Kondo, A. (2008). Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast. J Biochem 143(5): 667-674.
  40. Fukuda, N., Ishii, J., Tanaka, T., Fukuda, H., Ohnishi, N. and Kondo, A. (2008). Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles. Biotechnol Prog 24(2): 352-357.
  41. Shibasaki, S., Sakata, K., Ishii, J., Kondo, A. and Ueda, M. (2008). Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives. Appl Microbiol Biotechnol 80(4): 735-743.
  42. Fukuda, N., Ishii, J., Shibasaki, S., Ueda, M., Fukuda, H. and Kondo, A. (2007). High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions. Appl Microbiol Biotechnol 76(1): 151-158.
  43. Shibasaki, S., Kawabata, A., Ishii, J., Yagi, S., Kadonosono, T., Kato, M., Fukuda, N., Kondo, A. and Ueda, M. (2007). Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. Appl Microbiol Biotechnol 75(4): 821-828.
  44. Ishii, J., Matsumura, S., Kimura, S., Tatematsu, K., Kuroda, S., Fukuda, H. and Kondo, A. (2006). Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein. Biotechnol Prog 22(4): 954-960.
Protocols by Jun Ishii
  1. Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast