Ph.D. in Engineering, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan, 2014
Technology Research Association of Highly Efficient Gene Design (TRAHED) (04/2014-present)
Nakamura, Y., Ishii, J. and Kondo, A. (2014). Construction of a yeast-based signaling biosensor for human angiotensin II type 1 receptor via functional coupling between Asn295-mutated receptor and Gpa1/G chimeric Galpha. Biotechnol Bioeng.
Nakamura, Y., Takemoto, N., Ishii, J. and Kondo, A. (2014). Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system. Biotechnol Bioeng 111(3): 586-596.
Nakamura, Y., Ishii, J. and Kondo, A. (2013). Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells. PLoS One 8(12): e82237.
Nakamura, Y., Ishii, J. and Kondo, A. (2013). Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System. PLoS One 8(6): e66793.
Protocols by Yasuyuki Nakamura
Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast