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Albert Boronat

Education

Ph.D in Biochemistry, Department of Biochemistry, University of Barcelona, 1981

Current position

Professor, Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Spain
Group leader, Centre for Research in Agricultural Genomics, CSIC-IRTA-UAB-UB, Spain

Publications (since 2004)

  1. Angaman, D. M., Renato, M., Azcón-Bieto, J. and Boronat, A. (2014). Oxygen consumption and lipoxygenase activity in isolated tomato fruit chromoplasts. Journal of Plant Sciences 2(1): 5-8.
  2. Campos, N., Arro, M., Ferrer, A. and Boronat, A. (2014). Determination of 3-hydroxy-3-methylglutaryl CoA reductase activity in plants. Methods Mol Biol 1153: 21-40.
  3. Querol-Audí, J., Boronat, A., Centelles, J. J. and Imperial, S. (2014). Catalytically Important Residues in E. coli 1-Deoxy-D-Xylulose 5-Phosphate Synthase. Journal of Biosciences and Medicines 2(04): 30.
  4. Carretero-Paulet, L., Cairo, A., Talavera, D., Saura, A., Imperial, S., Rodriguez-Concepcion, M., Campos, N. and Boronat, A. (2013). Functional and evolutionary analysis of DXL1, a non-essential gene encoding a 1-deoxy-D-xylulose 5-phosphate synthase like protein in Arabidopsis thaliana. Gene 524(1): 40-53.
  5. Rodríguez-Concepción, M. and Boronat, A. (2013). Isoprenoid biosynthesis in prokaryotic organisms. In: Isoprenoid Synthesis in Plants and Microorganisms. Springer: 1-16.
  6. Rodríguez-Concepción, M., Campos, N., Ferrer, A. and Boronat, A. (2013). Biosynthesis of isoprenoid precursors in Arabidopsis. In: Isoprenoid Synthesis in Plants and Microorganisms. Springer: 439-456.
  7. Pateraki, I., Renato, M., Azcon-Bieto, J. and Boronat, A. (2013). An ATP synthase harboring an atypical gamma-subunit is involved in ATP synthesis in tomato fruit chromoplasts. Plant J 74(1): 74-85.
  8. Angaman, D. M., Pateraki, I., Busquets, M. and Boronat, A. (2013). Biosynthesis of lipophilic compounds in tomato fruit. International Journal of Biological and Chemical Sciences 7(3): 884-891.
  9. Angaman, D. M., Petrizzo, R., Hernandez-Gras, F., Romero-Segura, C., Pateraki, I., Busquets, M. and Boronat, A. (2012). Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts. Plant Methods 8(1): 1.
  10. Perez-Gil, J., Uros, E. M., Sauret-Gueto, S., Lois, L. M., Kirby, J., Nishimoto, M., Baidoo, E. E., Keasling, J. D., Boronat, A. and Rodriguez-Concepcion, M. (2012). Mutations in Escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway. PLoS One 7(8): e43775.
  11. Leivar, P., Antolin-Llovera, M., Ferrero, S., Closa, M., Arro, M., Ferrer, A., Boronat, A. and Campos, N. (2011). Multilevel control of Arabidopsis 3-hydroxy-3-methylglutaryl coenzyme A reductase by protein phosphatase 2A. Plant Cell 23(4): 1494-1511.
  12. Antolin-Llovera, M., Leivar, P., Arro, M., Ferrer, A., Boronat, A. and Campos, N. (2011). Modulation of plant HMG-CoA reductase by protein phosphatase 2A: positive and negative control at a key node of metabolism. Plant Signal Behav 6(8): 1127-1131.
  13.  Pérez-Gil, J., Bergua, M., Boronat, A. and Imperial, S. (2010). Cloning and functional characterization of an enzyme from Helicobacter pylori that catalyzes two steps of the methylerythritol phosphate pathway for isoprenoid biosynthesis. Biochimica et Biophysica Acta (BBA)-General Subjects 1800(9): 919-928.
  14. Boronat, A. (2010). The methylerythritol 4-phosphate pathway: regulatory role in plastid isoprenoid biosynthesis. In: The Chloroplast. Springer: 119-126..
  15. Gerber, E., Hemmerlin, A., Hartmann, M., Heintz, D., Hartmann, M. A., Mutterer, J., Rodriguez-Concepcion, M., Boronat, A., Van Dorsselaer, A., Rohmer, M., Crowell, D. N. and Bach, T. J. (2009). The plastidial 2-C-methyl-D-erythritol 4-phosphate pathway provides the isoprenyl moiety for protein geranylgeranylation in tobacco BY-2 cells. Plant Cell 21(1): 285-300.
  16. Busquets, A., Keim, V., Closa, M., del Arco, A., Boronat, A., Arro, M. and Ferrer, A. (2008). Arabidopsis thaliana contains a single gene encoding squalene synthase. Plant Mol Biol 67(1-2): 25-36.
  17. Phillips, M. A., Leon, P., Boronat, A. and Rodriguez-Concepcion, M. (2008). The plastidial MEP pathway: unified nomenclature and resources. Trends Plant Sci 13(12): 619-623.
  18. Ahumada, I., Cairó, A., Hemmerlin, A., González, V., Pateraki, I., Bach, T. J., Rodríguez-Concepción, M., Campos, N. and Boronat, A. (2008). Characterisation of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis. Functional Plant Biology 35(11): 1100-1111.
  19. Boronat, A. (2007). Regulatory role of the methylerythritol 4-phosphate pathway in plastid isoprenoid biosynthesis. Current Advances in the Biochemistry and Cell Biology of Plant Lipids: 273.
  20. Sauret-Gueto, S., Uros, E. M., Ibanez, E., Boronat, A. and Rodriguez-Concepcion, M. (2006). A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase. FEBS Lett 580(3): 736-740.
  21. Sauret-Gueto, S., Botella-Pavia, P., Flores-Perez, U., Martinez-Garcia, J. F., San Roman, C., Leon, P., Boronat, A. and Rodriguez-Concepcion, M. (2006). Plastid cues posttranscriptionally regulate the accumulation of key enzymes of the methylerythritol phosphate pathway in Arabidopsis. Plant Physiol 141(1): 75-84.
  22. Carretero-Paulet, L., Cairo, A., Botella-Pavia, P., Besumbes, O., Campos, N., Boronat, A. and Rodriguez-Concepcion, M. (2006). Enhanced flux through the methylerythritol 4-phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase. Plant Mol Biol 62(4-5): 683-695.
  23. Leivar, P., Gonzalez, V. M., Castel, S., Trelease, R. N., Lopez-Iglesias, C., Arro, M., Boronat, A., Campos, N., Ferrer, A. and Fernandez-Busquets, X. (2005). Subcellular localization of Arabidopsis 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Plant Physiol 137(1): 57-69.
  24. Bernal, C., Mendez, E., Terencio, J., Boronat, A. and Imperial, S. (2005). A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity. Anal Biochem 340(2): 245-251.
  25. Bernal, C., Palacin, C., Boronat, A. and Imperial, S. (2005). A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity. Anal Biochem 337(1): 55-61.
  26. Querol, J., Perez-Gil, J., Giménez, V., Boronat, A. and Imperial, S. (2005). Determination of 1-deoxy-D-xilulose 5-phosphate synthase activity: a comparison of different assays. Recent Research Developments in Analytical Biochemistry, 4: 1-16.
  27. Enfissi, E. M., Fraser, P. D., Lois, L. M., Boronat, A., Schuch, W. and Bramley, P. M. (2005). Metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato. Plant Biotechnol J 3(1): 17-27.
  28. Besumbes, O., Sauret-Gueto, S., Phillips, M. A., Imperial, S., Rodriguez-Concepcion, M. and Boronat, A. (2004). Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol. Biotechnol Bioeng 88(2): 168-175.
  29. Botella-Pavia, P., Besumbes, O., Phillips, M. A., Carretero-Paulet, L., Boronat, A. and Rodriguez-Concepcion, M. (2004). Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors. Plant J 40(2): 188-199.
  30. Manzano, D., Fernandez-Busquets, X., Schaller, H., Gonzalez, V., Boronat, A., Arro, M. and Ferrer, A. (2004). The metabolic imbalance underlying lesion formation in Arabidopsis thaliana overexpressing farnesyl diphosphate synthase (isoform 1S) leads to oxidative stress and is triggered by the developmental decline of endogenous HMGR activity. Planta 219(6): 982-992.
  31. Rodriguez-Concepcion, M., Fores, O., Martinez-Garcia, J. F., Gonzalez, V., Phillips, M. A., Ferrer, A. and Boronat, A. (2004). Distinct light-mediated pathways regulate the biosynthesis and exchange of isoprenoid precursors during Arabidopsis seedling development. Plant Cell 16(1): 144-156.

Protocols by Albert Boronat
  1. Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels