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Z. Hong Zhou


Ph.D. in Biochemistry, Baylor College of Medicine (BCM), Houston, TX, 1995

Current position

Professor, Department of Microbiology, Immunology and Molecular Genetics at the David Geffen School of Medicine, UCLA
Founding Director, Electron Imaging Center for Nanomachines (EICN), California NanoSystems Institute (CNSI), UCLA

Publications (since 2009)

  1. Zhou, Z. H.*, Hui, W. H., Shah, S., Jih, J., O’Connor, C. M., Sherman, M. B., Kedes, D. H. and Schein, S. (2014). Four levels of hierarchical organization including non-covalent chainmail brace the mature tumor herpesvirus capsid against pressurization. Structure. (In press) (*Corresponding author)
  2. Zhou, Z. H. (2014). Structures of viral membrane proteins by high-resolution cryoEM. Curr Opin Virol 5: 111-119.
  3. Ge, P. and Zhou, Z. H. (2014). Chaperone fusion proteins aid entropy-driven maturation of class II viral fusion proteins. Trends Microbiol 22(2): 100-106.
  4. Yang, K., Wills, E., Lim, H. Y., Zhou, Z. H. and Baines, J. D. (2014). Association of herpes simplex virus pUL31 with capsid vertices and components of the capsid vertex-specific complex. J Virol 88(7): 3815-3825.
  5. Gurel, P. S., Ge, P., Grintsevich, E. E., Shu, R., Blanchoin, L., Zhou, Z. H., Reisler, E. and Higgs, H. N. (2014). INF2-mediated severing through actin filament encirclement and disruption. Curr Biol 24(2): 156-164.
  6. Yan, X., Cardone, G., Zhang, X., Zhou, Z. H. and Baker, T. S. (2014). Single particle analysis integrated with microscopy: a high-throughput approach for reconstructing icosahedral particles. J Struct Biol 186(1): 8-18.
  7. Zhang, X., Ge, P., Yu, X., Brannan, J. M., Bi, G., Zhang, Q., Schein, S. and Zhou, Z. H. (2013). Cryo-EM structure of the mature dengue virus at 3.5-A resolution. Nat Struct Mol Biol 20(1): 105-110. (Featured on cover)
  8. Jiang, J., Miracco, E. J., Hong, K., Eckert, B., Chan, H., Cash, D. D., Min, B., Zhou, Z. H.*, Collins, K.* and Feigon, J.* (2013). The architecture of Tetrahymena telomerase holoenzyme. Nature 496(7444): 187-192. (*Corresponding authors)
  9. Zhang, X., Guo, H., Jin, L., Czornyj, E., Hodes, A., Hui, W. H., Nieh, A. W., Miller, J. F. and Zhou, Z. H. (2013). A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution. Elife (Cambridge) 2: e01299.
  10. Dai, X., Yu, X., Gong, H., Jiang, X., Abenes, G., Liu, H., Shivakoti, S., Britt, W. J., Zhu, H., Liu, F. and Zhou, Z. H. (2013). The smallest capsid protein mediates binding of the essential tegument protein pp150 to stabilize DNA-containing capsids in human cytomegalovirus. PLoS Pathog 9(8): e1003525.
  11. Hong, K., Upton, H., Miracco, E. J., Jiang, J., Zhou, Z. H., Feigon, J. and Collins, K. (2013). Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub. Mol Cell Biol 33(19): 3962-3971.
  12. Lu, G., Ota, A., Ren, S., Franklin, S., Rau, C. D., Ping, P., Lane, T. F., Zhou, Z. H., Reue, K., Lusis, A. J., Vondriska, T. and Wang, Y. (2013). PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response. Mol Metab 2(4): 405-416.
  13. Hui, W. H., Tang, Q., Liu, H., Atanasov, I., Liu, F., Zhu, H. and Zhou, Z. H. (2013). Protein interactions in the murine cytomegalovirus capsid revealed by cryoEM. Protein Cell 4(11): 833-845.
  14. Slaymaker, I. M., Fu, Y., Toso, D. B., Ranatunga, N., Brewster, A., Forsburg, S. L., Zhou, Z. H. and Chen, X. S. (2013). Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology. Nucleic Acids Res 41(5): 3446-3456.
  15. Wang, L. J. and Zhou, Z. H. (2013). Seeing molecular interactions of large complexes by cryoEM at atomic resolution. American Laboratory, 46:23-25.
  16. Yu, X., Jin, L., Jih, J., Shih, C. and Zhou, Z. H. (2013). 3.5A cryoEM structure of hepatitis B virus core assembled from full-length core protein. PLoS One 8(9): e69729.
  17. Jiang, J., Magilnick, N., Tsirulnikov, K., Abuladze, N., Atanasov, I., Ge, P., Narla, M., Pushkin, A.*, Zhou, Z. H.* and Kurtz, I.* (2013). Single particle electron microscopy analysis of the bovine anion exchanger 1 reveals a flexible linker connecting the cytoplasmic and membrane domains. PLoS One 8(2): e55408. (*Corresponding authors)
  18. Xiao, Q., Sohn, H., Chen, Z., Toso, D., Mechlenburg, M., Zhou, Z. H., Poirier, E., Dailly, A., Wang, H., Wu, Z., Cai, M. and Lu, Y. (2012). Mesoporous metal and metal alloy particles synthesized by aerosol-assisted confined growth of nanocrystals. Angew Chem Int Ed Engl 51(42): 10546-10550.
  19. Jiang, Z. K., Koh, S. B., Sato, M., Atanasov, I. C., Johnson, M., Zhou, Z. H., Deming, T. J. and Wu, L. (2013). Engineering polypeptide coatings to augment gene transduction and in vivo stability of adenoviruses. J Control Release 166(1): 75-85.
  20. Tao, C., Xia, C., Chen, X., Zhou, Z. H. and Bi, G. (2012). Ultrastructural analysis of neuronal synapses using state-of-the-art nano-imaging techniques. Neurosci Bull 28(4): 321-332.
  21. Zhang, X., Guo, H., Jin, L., Miller, J. and Zhou, H. (2012). Atomic structure of Bordetella Bacteriophage reveals a jellyroll fold in cement protein and a topologically distinct HK97-like fold in major capsid protein. Microscopy and Microanalysis 18(S2): 72-73.
  22. Huang, C. S., Ge, P., Zhou, Z. H. and Tong, L. (2012). An unanticipated architecture of the 750-kDa [agr] 6 [bgr] 6 holoenzyme of 3-methylcrotonyl-CoA carboxylase. Nature 481(7380): 219-223.
  23. Hughes, L. C., Ralston, K. S., Hill, K. L. and Zhou, Z. H. (2012). Three-dimensional structure of the Trypanosome flagellum suggests that the paraflagellar rod functions as a biomechanical spring. PLoS One 7(1): e25700.
  24. Luo, J., Obmolova, G., Malia, T. J., Wu, S. J., Duffy, K. E., Marion, J. D., Bell, J. K., Ge, P., Zhou, Z. H., Teplyakov, A., Zhao, Y., Lamb, R. J., Jordan, J. L., San Mateo, L. R., Sweet, R. W. and Gilliland, G. L. (2012). Lateral clustering of TLR3:dsRNA signaling units revealed by TLR3ecd:3Fabs quaternary structure. J Mol Biol 421(1): 112-124.
  25. Gopal, A., Zhou, Z. H., Knobler, C. M. and Gelbart, W. M. (2012). Visualizing large RNA molecules in solution. RNA 18(2): 284-299.
  26. Toso, D. B., Henstra, A. M., Gunsalus, R. P. and Zhou, Z. H. (2011). Structural, mass and elemental analyses of storage granules in methanogenic archaeal cells. Environ Microbiol 13(9): 2587-2599.
  27. Zhang, X. and Zhou, Z. H. (2011). Limiting factors in atomic resolution cryo electron microscopy: no simple tricks. J Struct Biol 175(3): 253-263.
  28. Yang, Z., Hu, W., Chen, K., Wang, J., Lux, R., Zhou, Z. H. and Shi, W. (2011). Alanine 32 in PilA is important for PilA stability and type IV pili function in Myxococcus xanthus. Microbiology 157(Pt 7): 1920-1928.
  29. Buehler, D. C., Toso, D. B., Kickhoefer, V. A., Zhou, Z. H. and Rome, L. H. (2011). Vaults engineered for hydrophobic drug delivery. Small 7(10): 1432-1439. (Featured on cover)
  30. Ge, P. and Zhou, Z. H. (2011). Hydrogen-bonding networks and RNA bases revealed by cryo electron microscopy suggest a triggering mechanism for calcium switches. Proc Natl Acad Sci U S A 108(23): 9637-9642.
  31. Meng, H., Yang, S., Li, Z., Xia, T., Chen, J., Ji, Z., Zhang, H., Wang, X., Lin, S., Huang, C., Zhou, Z. H., Zink, J. I. and Nel, A. E. (2011). Aspect ratio determines the quantity of mesoporous silica nanoparticle uptake by a small GTPase-dependent macropinocytosis mechanism. ACS Nano 5(6): 4434-4447.
  32. Chen, J., Sun, J., Atanasov, I., Ryazantsev, S. and Zhou, Z. H. (2011). Electron tomography reveals polyhedrin binding and existence of both empty and full cytoplasmic polyhedrosis virus particles inside infectious polyhedra. J Virol 85(12): 6077-6081.
  33. Yu, X., Ge, P., Jiang, J., Atanasov, I. and Zhou, Z. H. (2011). Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses. Structure 19(5): 652-661. (Featured on cover)
  34. Gangalum, R. K., Atanasov, I. C., Zhou, Z. H. and Bhat, S. P. (2011). AlphaB-crystallin is found in detergent-resistant membrane microdomains and is secreted via exosomes from human retinal pigment epithelial cells. J Biol Chem 286(5): 3261-3269.
  35. Yu, X., Shah, S., Lee, M., Dai, W., Lo, P., Britt, W., Zhu, H., Liu, F. and Zhou, Z. H. (2011). Biochemical and structural characterization of the capsid-bound tegument proteins of human cytomegalovirus. J Struct Biol 174(3): 451-460.
  36. Green, T. J., Rowse, M., Tsao, J., Kang, J., Ge, P., Zhou, Z. H. and Luo, M. (2011). Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus. J Virol 85(6): 2714-2722.
  37. Liu, H., Wu, L. and Zhou, Z. H. (2011). Model of the trimeric fiber and its interactions with the pentameric penton base of human adenovirus by cryo-electron microscopy. J Mol Biol 406(5): 764-774. (Featured on cover)
  38. Leong, P. A., Yu, X., Zhou, Z. H. and Jensen, G. J. (2010). Correcting for the ewald sphere in high-resolution single-particle reconstructions. Methods Enzymol 482: 369-380.
  39. Liu, J., Howell, J. K., Bradley, S. D., Zheng, Y., Zhou, Z. H. and Norris, S. J. (2010). Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography. J Mol Biol 403(4): 546-561. (Featured on cover)
  40. Liu, S., He, J., Shih, C., Li, K., Dai, A., Zhou, Z. H. and Zhang, J. (2010). Structural comparisons of hepatitis B core antigen particles with different C-terminal lengths. Virus Res 149(2): 241-244.
  41. Jin, L., Pan, C., Qi, Z., Zhou, Z. H. and Jiang, S. (2010). Fab crystallization and preliminary X-ray analysis of NC-1, an anti-HIV-1 antibody that recognizes the six-helix bundle core of gp41. Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 7): 854-857.
  42. Liu, H., Jin, L., Koh, S. B., Atanasov, I., Schein, S., Wu, L. and Zhou, Z. H. (2010). Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks. Science 329(5995): 1038-1043.
  43. Huang, C. S., Sadre-Bazzaz, K., Shen, Y., Deng, B., Zhou, Z. H. and Tong, L. (2010). Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase. Nature 466(7309): 1001-1005.
  44. Zhang, X., Zhang, X. and Zhou, Z. H. (2010). Low cost, high performance GPU computing solution for atomic resolution cryoEM single-particle reconstruction. J Struct Biol 172(3): 400-406.
  45. Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z. H.* and Guo, F.* (2010). DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA 16(8): 1570-1583. (*Corresponding authors)
  46. Zhang, X., Jin, L., Fang, Q., Hui, W. H. and Zhou, Z. H. (2010). 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry. Cell 141(3): 472-482. (Featured on cover and Cell PaperFlick Video of the issue)
  47. Zhu, J., Cheng, L., Fang, Q., Zhou, Z. H.* and Honig, B.* (2010). Building and refining protein models within cryo-electron microscopy density maps based on homology modeling and multiscale structure refinement. J Mol Biol 397(3): 835-851. (Featured on cover) (*Corresponding authors)
  48. Zhang, X., Boyce, M., Bhattacharya, B., Zhang, X., Schein, S., Roy, P. and Zhou, Z. H. (2010). Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins. Proc Natl Acad Sci U S A 107(14): 6292-6297.
  49. Cheng, L., Zhu, J., Hui, W. H., Zhang, X., Honig, B., Fang, Q. and Zhou, Z. H. (2010). Backbone model of an aquareovirus virion by cryo-electron microscopy and bioinformatics. J Mol Biol 397(3): 852-863. (Featured on cover)
  50. Dai, W., Hodes, A., Hui, W. H., Gingery, M., Miller, J. F. and Zhou, Z. H. (2010). Three-dimensional structure of tropism-switching Bordetella bacteriophage. Proc Natl Acad Sci U S A 107(9): 4347-4352.
  51. Ge, P., Tsao, J., Schein, S., Green, T. J., Luo, M. and Zhou, Z. H. (2010). Cryo-EM model of the bullet-shaped vesicular stomatitis virus. Science 327(5966): 689-693.
  52. Peng, L., Ryazantsev, S., Sun, R. and Zhou, Z. H. (2010). Three-dimensional visualization of gammaherpesvirus life cycle in host cells by electron tomography. Structure 18(1): 47-58. (Featured on cover and a Preview of Structure)
  53. Yan, M., Du, J., Gu, Z., Liang, M., Hu, Y., Zhang, W., Priceman, S., Wu, L., Zhou, Z. H., Liu, Z., Segura, T., Tang, Y. and Lu, Y. (2010). A novel intracellular protein delivery platform based on single-protein nanocapsules. Nat Nanotechnol 5(1): 48-53.
  54. Guo, H., Wang, L., Peng, L., Zhou, Z. H. and Deng, H. (2009). Open reading frame 33 of a gammaherpesvirus encodes a tegument protein essential for virion morphogenesis and egress. J Virol 83(20): 10582-10595.
  55. Li, F., Ge, P., Hui, W. H., Atanasov, I., Rogers, K., Guo, Q., Osato, D., Falick, A. M., Zhou, Z. H. and Simpson, L. (2009). Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria. Proc Natl Acad Sci U S A 106(30): 12306-12310.
  56. Pan, D., Wang, X., Zhou, Z. H., Chen, W., Xu, C. and Lu, Y. (2009). Synthesis of quaternary semiconductor nanocrystals with tunable band gaps. Chemistry of Materials 21(12): 2489-2493.
  57. Nayak, D. P., Balogun, R. A., Yamada, H., Zhou, Z. H. and Barman, S. (2009). Influenza virus morphogenesis and budding. Virus Res 143(2): 147-161.

Book chapters and invited commentaries

  1. Zhou, Z. H. (2012). Seeing engineered loops in a gene delivery vehicle by cryoEM. Structure 20(8): 1286-1288.
  2. Zhou, Z. H. (2011). Atomic resolution cryo electron microscopy of macromolecular complexes. Adv Protein Chem Struct Biol 82: 1-35.
  3. Luo, M., Green T. J. and Zhou, Z. H. (2011) Assembly of vesicular stomatitis virus, Chapter 9. In: Luo, M. (ed). Negative Strand RNA Virus. World Scientific.
Protocols by Z. Hong Zhou
  1. Purification of Herpesvirus Virions and Capsids