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Zongtian Tong


Ph.D., Department of Biology, University of Pennsylvania, 2008

Current position

She is a Postdoctoral Fellow in the School of Medicine at Johns Hopkins University. Her current research focuses on the identification of E3 ubiquitin ligase substrates.


  1. Stewart, E. V., Nwosu, C. C., Tong, Z., Roguev, A., Cummins, T. D., Kim, D. U., Hayles, J., Park, H. O., Hoe, K. L., Powell, D. W., Krogan, N. J. and Espenshade, P. J. (2011). Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex. Mol Cell 42(2): 160-171.
  2. Tong, Z., Gao, X. D., Howell, A. S., Bose, I., Lew, D. J. and Bi, E. (2007). Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J Cell Biol 179(7): 1375-1384.
  3. Gao, X. D., Sperber, L. M., Kane, S. A., Tong, Z., Tong, A. H., Boone, C. and Bi, E. (2007). Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol Biol Cell 18(7): 2542-2560.
  4. Iwase, M., Luo, J., Nagaraj, S., Longtine, M., Kim, H. B., Haarer, B. K., Caruso, C., Tong, Z., Pringle, J. R. and Bi, E. (2006). Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site. Mol Biol Cell 17(3): 1110-1125.
Protocols by Zongtian Tong
  1. 32P Radioactive Probe Synthesis and Preparation
  2. [Bio101] Purification of 6x His-tagged Protein (from E. coli)
  3. [Bio101] Colony PCR Using Yeast Spheroplasted Cells
  4. [Bio101] How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
  5. [Bio101] Large Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
  6. [Bio101] Metabolic Labeling of Yeast Proteins
  7. [Bio101] Protocol for Whole Cell Lysis of Yeast
  8. [Bio101] Small Scale Native Affinity Purifications of Solubilized Membrane Proteins from Yeast
  9. Spot Assay for Yeast
  10. [Bio101] TAP Purification of Yeast Proteins
  11. [Bio101] Yeast Vacuole Staining with FM4-64
  12. [Bio101] RNA Preparation for Microarray Experiments
  13. Subcellular Fractionation Using Accudenz Gradient to Separate ER/Golgi in Yeast