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Satoshi Nakajima


Ph.D., Tohoku University, 2000

Current position

Assistant Professor, Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh

Publications (since 2003)

  1. Nakajima, S.*, Lan, L., Wei, L., Hsieh, C. L., Rapic-Otrin, V., Yasui, A. and Levine, A. S. (2014). Ubiquitin-specific protease 5 is required for the efficient repair of DNA double-strand breaks. PLoS One 9(1): e84899. (*Co-respondence)
  2. Lan, L.**, Nakajima, S.*, Wei, L.*, Sun, L., Hsieh, C. L., Sobol, R. W., Bruchez, M., Van Houten, B., Yasui, A. and Levine, A. S. (2014). Novel method for site-specific induction of oxidative DNA damage reveals differences in recruitment of repair proteins to heterochromatin and euchromatin. Nucleic Acids Res 42(4): 2330-2345. (*Co-first, **Co-respondence)
  3. Xu, L., Chen, Y.-C., Nakajima, S., Chong, J., Wang, L., Lan, L., Zhang, C. and Wang, D. (2014). A chemical probe targets DNA 5-formylcytosine sites and inhibits TDG excision, polymerases bypass, and gene expression. Chemical Science 5(2): 567-574.
  4. Benitez, A., Yuan, F., Nakajima, S., Wei, L., Qian, L., Myers, R., Hu, J. J., Lan, L. and Zhang, Y. (2014). Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res 42(3): 1671-1683.
  5. Ui, A., Ogiwara, H., Nakajima, S., Kanno, S., Watanabe, R., Harata, M., Okayama, H., Harris, C. C., Yokota, J., Yasui, A. and Kohno, T. (2014). Possible involvement of LKB1-AMPK signaling in non-homologous end joining. Oncogene 33(13): 1640-1648.
  6. Wei, L., Nakajima, S., Hsieh, C. L., Kanno, S., Masutani, M., Levine, A. S., Yasui, A. and Lan, L. (2013). Damage response of XRCC1 at sites of DNA single strand breaks is regulated by phosphorylation and ubiquitylation after degradation of poly(ADP-ribose). J Cell Sci 126(Pt 19): 4414-4423.
  7. Lan, L., Nakajima, S.*, Kapetanaki, M. G., Hsieh, C. L., Fagerburg, M., Thickman, K., Rodriguez-Collazo, P., Leuba, S. H., Levine, A. S. and Rapic-Otrin, V. (2012). Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase. J Biol Chem 287(15): 12036-12049. (*Co-first author)
  8. Zlatanou, A., Despras, E., Braz-Petta, T., Boubakour-Azzouz, I., Pouvelle, C., Stewart, G. S., Nakajima, S., Yasui, A., Ishchenko, A. A. and Kannouche, P. L. (2011). The hMsh2-hMsh6 complex acts in concert with monoubiquitinated PCNA and Pol eta in response to oxidative DNA damage in human cells. Mol Cell 43(4): 649-662.
  9. Ogiwara, H., Ui, A., Otsuka, A., Satoh, H., Yokomi, I., Nakajima, S., Yasui, A., Yokota, J. and Kohno, T. (2011). Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene 30(18): 2135-2146.
  10. Lan, L., Ui, A., Nakajima, S., Hatakeyama, K., Hoshi, M., Watanabe, R., Janicki, S. M., Ogiwara, H., Kohno, T., Kanno, S. and Yasui, A. (2010). The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell 40(6): 976-987.
  11. Ziv, O., Geacintov, N., Nakajima, S., Yasui, A. and Livneh, Z. (2009). DNA polymerase zeta cooperates with polymerases kappa and iota in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients. Proc Natl Acad Sci U S A 106(28): 11552-11557.
  12. Petta, T. B., Nakajima, S., Zlatanou, A., Despras, E., Couve-Privat, S., Ishchenko, A., Sarasin, A., Yasui, A. and Kannouche, P. (2008). Human DNA polymerase iota protects cells against oxidative stress. EMBO J 27(21): 2883-2895.
  13. Hong, Z., Jiang, J., Lan, L., Nakajima, S., Kanno, S., Koseki, H. and Yasui, A. (2008). A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell. Nucleic Acids Res 36(9): 2939-2947.
  14. Kanno, S., Kuzuoka, H., Sasao, S., Hong, Z., Lan, L., Nakajima, S. and Yasui, A. (2007). A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. EMBO J 26(8): 2094-2103.
  15. Kamath-Loeb, A. S., Lan, L., Nakajima, S., Yasui, A. and Loeb, L. A. (2007). Werner syndrome protein interacts functionally with translesion DNA polymerases. Proc Natl Acad Sci U S A 104(25): 10394-10399.
  16. Nakajima, S., Lan, L., Kanno, S., Usami, N., Kobayashi, K., Mori, M., Shiomi, T. and Yasui, A. (2006). Replication-dependent and -independent responses of RAD18 to DNA damage in human cells. J Biol Chem 281(45): 34687-34695.
  17. Lo, H. L., Nakajima, S., Ma, L., Walter, B., Yasui, A., Ethell, D. W. and Owen, L. B. (2005). Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest. BMC Cancer 5: 135.
  18. Lan, L., Nakajima, S., Komatsu, K., Nussenzweig, A., Shimamoto, A., Oshima, J. and Yasui, A. (2005). Accumulation of Werner protein at DNA double-strand breaks in human cells. J Cell Sci 118(Pt 18): 4153-4162.
  19. Jans, J., Schul, W., Sert, Y. G., Rijksen, Y., Rebel, H., Eker, A. P., Nakajima, S., van Steeg, H., de Gruijl, F. R., Yasui, A., Hoeijmakers, J. H. and van der Horst, G. T. (2005). Powerful skin cancer protection by a CPD-photolyase transgene. Curr Biol 15(2): 105-115.
  20. Nakajima, S., Lan, L., Kanno, S., Takao, M., Yamamoto, K., Eker, A. P. and Yasui, A. (2004). UV light-induced DNA damage and tolerance for the survival of nucleotide excision repair-deficient human cells. J Biol Chem 279(45): 46674-46677.
  21. Lan, L., Nakajima, S., Oohata, Y., Takao, M., Okano, S., Masutani, M., Wilson, S. H. and Yasui, A. (2004). In situ analysis of repair processes for oxidative DNA damage in mammalian cells. Proc Natl Acad Sci U S A 101(38): 13738-13743.
  22. Lan, L., Hayashi, T., Rabeya, R. M., Nakajima, S., Kanno, S., Takao, M., Matsunaga, T., Yoshino, M., Ichikawa, M., Riele, H., Tsuchiya, S., Tanaka, K. and Yasui, A. (2004). Functional and physical interactions between ERCC1 and MSH2 complexes for resistance to cis-diamminedichloroplatinum(II) in mammalian cells. DNA Repair (Amst) 3(2): 135-143.
  23. Hirouchi, T., Nakajima, S., Najrana, T., Tanaka, M., Matsunaga, T., Hidema, J., Teranishi, M., Fujino, T., Kumagai, T. and Yamamoto, K. (2003). A gene for a Class II DNA photolyase from Oryza sativa: cloning of the cDNA by dilution-amplification. Mol Genet Genomics 269(4): 508-516.
  24. Fitch, M. E., Nakajima, S., Yasui, A. and Ford, J. M. (2003). In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product. J Biol Chem 278(47): 46906-46910.
Protocols by Satoshi Nakajima
  1. Novel Method for Site-specific Induction of Oxidative DNA Damage to Study Recruitment of Repair Proteins to Heterochromatin and Euchromatin