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Toshitsugu Fujita

Education

Ph.D. in Science, Department of Biological Sciences, Graduate school of Science, Osaka University, Japan, 2004

Current position

Assistant professor, Office of combined program on microbiology and immunology, Research Institute for Microbial Diseases, Osaka University, Japan

Publications

  1. Fujita, T., Asano, Y., Ohtsuka, J., Takada, Y., Saito, K., Ohki, R. and Fujii, H. (2013). Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3: 3171.
  2. Fujita, T. and Fujii, H. (2013). Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun 439(1): 132-136.
  3. Fujita, T. and Fujii, H. (2013). Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation. ISRN Biochemistry 2013.
  4. Fujita, T. and Fujii, H. (2012). Efficient isolation of specific genomic regions by insertional chromatin immunoprecipitation (iChIP) with a second-generation tagged LexA DNA-binding domain. Advances in Bioscience & Biotechnology 3(5).
  5. Fujita, T. and Fujii, H. (2012). Transcription start sites and usage of the first exon of mouse Foxp3 gene. Mol Biol Rep 39(10): 9613-9619.
  6. Fujita, T. and Fujii, H. (2011). Direct identification of insulator components by insertional chromatin immunoprecipitation. PLoS One 6(10): e26109.
  7. Fujita, T. and Fujii, H. (2011). Species-specific 5'-genomic structure and multiple transcription start sites in the chicken Pax5 gene. Gene 477(1-2): 24-31.
  8. Fujita, T., Piuz, I. and Schlegel, W. (2010). Transcription elongation factors are involved in programming hormone production in pituitary neuroendocrine GH4C1 cells. Mol Cell Endocrinol 319(1-2): 63-70.
  9. Yamazaki, T., Walchli, S., Fujita, T., Ryser, S., Hoshijima, M., Schlegel, W., Kuroda, S. and Maturana, A. D. (2010). Splice variants of enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy. Cardiovasc Res 86(3): 374-382.
  10. Maturana, A. D., Fujita, T. and Kuroda, S. (2010). Functions of fasciculation and elongation protein zeta-1 (FEZ1) in the brain. ScientificWorldJournal 10: 1646-1654.
  11. Fujita, T. and Schlegel, W. (2010). Promoter-proximal pausing of RNA polymerase II: an opportunity to regulate gene transcription. J Recept Signal Transduct Res 30(1): 31-42.
  12. Fujita, T., Piuz, I. and Schlegel, W. (2009). The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner. FEBS Lett 583(17): 2893-2898.
  13. Fujita, T., Piuz, I. and Schlegel, W. (2009). Negative elongation factor NELF controls transcription of immediate early genes in a stimulus-specific manner. Exp Cell Res 315(2): 274-284.
  14. Charital, Y. M., van Haasteren, G., Massiha, A., Schlegel, W. and Fujita, T. (2009). A functional NF-kappaB enhancer element in the first intron contributes to the control of c-fos transcription. Gene 430(1-2): 116-122.
  15. Fujita, T., Ryser, S., Piuz, I. and Schlegel, W. (2008). Up-regulation of P-TEFb by the MEK1-extracellular signal-regulated kinase signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells. Mol Cell Biol 28(5): 1630-1643.
  16. Fujita, T., Maturana, A. D., Ikuta, J., Hamada, J., Walchli, S., Suzuki, T., Sawa, H., Wooten, M. W., Okajima, T., Tatematsu, K., Tanizawa, K. and Kuroda, S. (2007). Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells. Biochem Biophys Res Commun 361(3): 605-610.
  17. Ikuta, J., Maturana, A., Fujita, T., Okajima, T., Tatematsu, K., Tanizawa, K. and Kuroda, S. (2007). Fasciculation and elongation protein zeta-1 (FEZ1) participates in the polarization of hippocampal neuron by controlling the mitochondrial motility. Biochem Biophys Res Commun 353(1): 127-132.
  18. Fujita, T., Ryser, S., Tortola, S., Piuz, I. and Schlegel, W. (2007). Gene-specific recruitment of positive and negative elongation factors during stimulated transcription of the MKP-1 gene in neuroendocrine cells. Nucleic Acids Res 35(3): 1007-1017.
  19. Ryser, S., Fujita, T., Tortola, S., Piuz, I. and Schlegel, W. (2007). The rate of c-fos transcription in vivo is continuously regulated at the level of elongation by dynamic stimulus-coupled recruitment of positive transcription elongation factor b. J Biol Chem 282(7): 5075-5084.
  20. Suzuki, T., Okada, Y., Semba, S., Orba, Y., Yamanouchi, S., Endo, S., Tanaka, S., Fujita, T., Kuroda, S., Nagashima, K. and Sawa, H. (2005). Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation. J Biol Chem 280(26): 24948-24956.
  21. Fujita, T., Ikuta, J., Hamada, J., Okajima, T., Tatematsu, K., Tanizawa, K. and Kuroda, S. (2004). Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1. Biochem Biophys Res Commun 313(3): 738-744.
  22. Fujita, T., Ikuta, J., Maturana, A., Okajima, T., Tatematsu, K., Tanizawa, K., and Kuroda, S. (2004). UNC-76/FEZ family is essential for axonal guidance. Recent Res. Devel. Biophys. Biochem. 4, 313-320.
  23. Miyoshi, K., Honda, A., Baba, K., Taniguchi, M., Oono, K., Fujita, T., Kuroda, S., Katayama, T. and Tohyama, M. (2003). Disrupted-In-Schizophrenia 1, a candidate gene for schizophrenia, participates in neurite outgrowth. Mol Psychiatry 8(7): 685-694.
Protocols by Toshitsugu Fujita
  1. Identification of Proteins Interacting with Genomic Regions of Interest in vivo Using Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (enChIP)