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Masaru Ohme-Takagi


Ph.D. in Plant Molecular Biology, Department of Biology, Nagoya University, Japan, 1985

Current position

Director/Professor, Institute for Environmental Science and Technology (IEST), Saitama University, Saitama 338-8570, Japan
Invited researcher, Plant Gene Regulation Research Group, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, Higashi 1-1-1, Tsukuba, Ibaraki 305-8562, Japan

Publications (2008-2014)

  1. Ohme-Takagi, M. and Tada, Y. (2014). Involvement of elevated proline accumulation in enhanced osmotic stress tolerance in conferred by chimeric repressor gene silencing technology. Plant Signal Behav 9(2). (Epub ahead of print)
  2. Fujiwara, S., Mitsuda, N., Nakai, Y., Kigoshi, K., Suzuki, K. and Ohme-Takagi, M. (2014). Chimeric repressor analysis identifies MYB87 as a possible regulator of morphogenesis via cell wall organization and remodeling in Arabidopsis. Biotechnol Lett (Epub ahead of print)
  3. Sewelam, N., Oshima, Y., Mitsuda, N. and Ohme-Takagi, M. (2014). A Step towards Understanding Plant Responses to Multiple Environmental Stresses: a Genome-wide Study. Plant Cell Environ (Epub ahead of print)
  4. Yoshida, K., Sakamoto, S., Kawai, T., Kobayashi, Y., Sato, K., Ichinose, Y., Yaoi, K., Akiyoshi-Endo, M., Sato, H., Takamizo, T., Ohme-Takagi, M. and Mitsuda, N. (2013). Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation. Front Plant Sci 4: 383.
  5. Nakata, M. and Ohme-Takagi, M. (2013). Two bHLH-type transcription factors, JA-ASSOCIATED MYC2-LIKE2 and JAM3, are transcriptional repressors and affect male fertility. Plant Signal Behav 8(12).
  6. Tanaka, Y., Oshima, Y., Yamamura, T., Sugiyama, M., Mitsuda, N., Ohtsubo, N., Ohme-Takagi, M. and Terakawa, T. (2013). Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression. Sci Rep 3: 2641.
  7. Licausi, F., Ohme-Takagi, M. and Perata, P. (2013). APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytol 199(3): 639-649.
  8. Oshima, Y., Shikata, M., Koyama, T., Ohtsubo, N., Mitsuda, N. and Ohme-Takagi, M. (2013). MIXTA-like transcription factors and WAX INDUCER1/SHINE1 coordinately regulate cuticle development in Arabidopsis and Torenia fournieri. Plant Cell 25(5): 1609-1624.
  9. Nakata, M., Mitsuda, N., Herde, M., Koo, A. J., Moreno, J. E., Suzuki, K., Howe, G. A. and Ohme-Takagi, M. (2013). A bHLH-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, acts as a repressor to negatively regulate jasmonate signaling in arabidopsis. Plant Cell 25(5): 1641-1656.
  10. Koyama, T., Nii, H., Mitsuda, N., Ohta, M., Kitajima, S., Ohme-Takagi, M. and Sato, F. (2013). A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol 162(2): 991-1005.
  11. Niwa, M., Daimon, Y., Kurotani, K., Higo, A., Pruneda-Paz, J. L., Breton, G., Mitsuda, N., Kay, S. A., Ohme-Takagi, M., Endo, M. and Araki, T. (2013). BRANCHED1 interacts with FLOWERING LOCUS T to repress the floral transition of the axillary meristems in Arabidopsis. Plant Cell 25(4): 1228-1242.
  12. Ikeda, M., Mitsuda, N. and Ohme-Takagi, M. (2013). ATBS1 INTERACTING FACTORs negatively regulate Arabidopsis cell elongation in the triantagonistic bHLH system. Plant Signal Behav 8(3): e23448.
  13. Lin, H., Niu, L., McHale, N. A., Ohme-Takagi, M., Mysore, K. S. and Tadege, M. (2013). Evolutionarily conserved repressive activity of WOX proteins mediates leaf blade outgrowth and floral organ development in plants. Proc Natl Acad Sci U S A 110(1): 366-371.
  14. King, G. J., Chanson, A. H., McCallum, E. J., Ohme-Takagi, M., Byriel, K., Hill, J. M., Martin, J. L. and Mylne, J. S. (2013). The Arabidopsis B3 domain protein VERNALIZATION1 (VRN1) is involved in processes essential for development, with structural and mutational studies revealing its DNA-binding surface. J Biol Chem 288(5): 3198-3207.
  15. Ikeda, M., Fujiwara, S., Mitsuda, N. and Ohme-Takagi, M. (2012). A triantagonistic basic helix-loop-helix system regulates cell elongation in Arabidopsis. Plant Cell 24(11): 4483-4497.
  16. Nakai, Y., Nakahira, Y., Sumida, H., Takebayashi, K., Nagasawa, Y., Yamasaki, K., Akiyama, M., Ohme-Takagi, M., Fujiwara, S., Shiina, T., Mitsuda, N., Fukusaki, E., Kubo, Y. and Sato, M. H. (2013). Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis. Plant J 73(5): 761-775.
  17. Pirrello, J., Prasad, B. C., Zhang, W., Chen, K., Mila, I., Zouine, M., Latche, A., Pech, J. C., Ohme-Takagi, M., Regad, F. and Bouzayen, M. (2012). Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene. BMC Plant Biol 12: 190.
  18. Todaka, D., Nakashima, K., Maruyama, K., Kidokoro, S., Osakabe, Y., Ito, Y., Matsukura, S., Fujita, Y., Yoshiwara, K., Ohme-Takagi, M., Kojima, M., Sakakibara, H., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2012). Rice phytochrome-interacting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress. Proc Natl Acad Sci U S A 109(39): 15947-15952.
  19. Kim, J. S., Mizoi, J., Kidokoro, S., Maruyama, K., Nakajima, J., Nakashima, K., Mitsuda, N., Takiguchi, Y., Ohme-Takagi, M., Kondou, Y., Yoshizumi, T., Matsui, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2012). Arabidopsis growth-regulating factor7 functions as a transcriptional repressor of abscisic acid- and osmotic stress-responsive genes, including DREB2A. Plant Cell 24(8): 3393-3405.
  20. Tanaka, W., Toriba, T., Ohmori, Y., Yoshida, A., Kawai, A., Mayama-Tsuchida, T., Ichikawa, H., Mitsuda, N., Ohme-Takagi, M. and Hirano, H. Y. (2012). The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet. Plant Cell 24(1): 80-95.
  21. Sato, H., Yoshida, K., Mitsuda, N., Ohme-Takagi, M. and Takamizo, T. (2012). Male-sterile and cleistogamous phenotypes in tall fescue induced by chimeric repressors of SUPERWOMAN1 and OsMADS58. Plant Sci 183: 183-189.
  22. Iwase, A., Ohme-Takagi, M. and Sugimoto, K. (2011). WIND1: a key molecular switch for plant cell dedifferentiation. Plant Signal Behav 6(12): 1943-1945.
  23. Ito, Y., Thirumurugan, T., Serizawa, A., Hiratsu, K., Ohme-Takagi, M. and Kurata, N. (2011). Aberrant vegetative and reproductive development by overexpression and lethality by silencing of OsHAP3E in rice. Plant Sci 181(2): 105-110.
  24. Ikeda, M., Mitsuda, N. and Ohme-Takagi, M. (2011). Arabidopsis HsfB1 and HsfB2b act as repressors of the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance. Plant Physiol 157(3): 1243-1254.
  25. Asahina, M., Azuma, K., Pitaksaringkarn, W., Yamazaki, T., Mitsuda, N., Ohme-Takagi, M., Yamaguchi, S., Kamiya, Y., Okada, K., Nishimura, T., Koshiba, T., Yokota, T., Kamada, H. and Satoh, S. (2011). Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis. Proc Natl Acad Sci U S A 108(38): 16128-16132.
  26. Ohtani, M., Nishikubo, N., Xu, B., Yamaguchi, M., Mitsuda, N., Goue, N., Shi, F., Ohme-Takagi, M. and Demura, T. (2011). A NAC domain protein family contributing to the regulation of wood formation in poplar. Plant J 67(3): 499-512.
  27. Mitsuda, N., Matsui, K., Ikeda, M., Nakata, M., Oshima, Y., Nagatoshi, Y. and Ohme-Takagi, M. (2011). CRES-T, an effective gene silencing system utilizing chimeric repressors. Methods Mol Biol 754: 87-105.
  28. Nishizawa-Yokoi, A., Nosaka, R., Hayashi, H., Tainaka, H., Maruta, T., Tamoi, M., Ikeda, M., Ohme-Takagi, M., Yoshimura, K., Yabuta, Y. and Shigeoka, S. (2011). HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress. Plant Cell Physiol 52(5): 933-945.
  29. Koyama, T., Ohme-Takagi, M. and Sato, F. (2011). Generation of serrated and wavy petals by inhibition of the activity of TCP transcription factors in Arabidopsis thaliana. Plant Signal Behav 6(5): 697-699.
  30. Mito, T., Seki, M., Shinozaki, K., Ohme-Takagi, M. and Matsui, K. (2011). Generation of chimeric repressors that confer salt tolerance in Arabidopsis and rice. Plant Biotechnol J 9(7): 736-746.
  31. Iwase, A., Mitsuda, N., Koyama, T., Hiratsu, K., Kojima, M., Arai, T., Inoue, Y., Seki, M., Sakakibara, H., Sugimoto, K. and Ohme-Takagi, M. (2011). The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis. Curr Biol 21(6): 508-514.
  32. Yamaguchi, M., Mitsuda, N., Ohtani, M., Ohme-Takagi, M., Kato, K. and Demura, T. (2011). VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation. Plant J 66(4): 579-590.
  33. Mitsuda, N., Takiguchi, Y., Shikata, M., SAGE, O., Ono, M., Sasaki, K., Yamaguchi, H., Narumi, T., Tanaka, Y. and Sugiyama, M. (2011). The new FioreDB database provides comprehensive information on plant transcription factors and phenotypes induced by CRES-T in ornamental and model plants. Plant biotechnology 28(2): 123-130.
  34. Narumi, T., Aida, R., Koyama, T., Yamaguchi, H., Sasaki, K., Shikata, M., Nakayama, M., Masaru, O. T. and Ohtsubo, N. (2011). Arabidopsis chimeric TCP3 repressor produces novel floral traits in Torenia fournieri and Chrysanthemum morifolium. Plant biotechnology 28(2): 131-140.
  35. Tanaka, Y., Yamamura, T., Oshima, Y., Mitsuda, N., Koyama, T., Masaru, O. T. and Terakawa, T. (2011). Creating ruffled flower petals in Cyclamen persicum by expression of the chimeric cyclamen TCP repressor. Plant biotechnology 28(2): 141-147.
  36. Gion, K., Suzuri, R., Shikata, M., Mitsuda, N., Oshima, Y., Koyama, T., Masaru, O. T., Ohtsubo, N. and Tanaka, Y. (2011). Morphological changes of Rosa× hybrida by a chimeric repressor of Arabidopsis TCP3. Plant biotechnology 28(2): 149-152.
  37. Sage-Ono, K., Ozeki, Y., Hiyama, S., Higuchi, Y., Kamada, H., Mitsuda, N., Masaru, O. T. and Ono, M. (2011). Induction of double flowers in Pharbitis nil using a class-C MADS-box transcription factor with Chimeric REpressor gene-Silencing Technology. Plant biotechnology 28(2): 153-165.
  38. Sasaki, K., Yamaguchi, H., Narumi, T., Shikata, M., Oshima, Y., Nakata, M., Mitsuda, N., Masaru, O. T. and Ohtsubo, N. (2011). Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind. Plant biotechnology 28(2): 181-188.
  39. Oshima, Y., Mitsuda, N., Nakata, M., Nakagawa, T., Nagaya, S., Kato, K. and Ohme-Takagi, M. (2011). Novel vector systems to accelerate functional analysis of transcription factors using chimeric repressor gene-silencing technology (CRES-T). Plant Biotechnol 28: 201-210.
  40. Koyama, T., Mitsuda, N., Seki, M., Shinozaki, K. and Ohme-Takagi, M. (2010). TCP transcription factors regulate the activities of ASYMMETRIC LEAVES1 and miR164, as well as the auxin response, during differentiation of leaves in Arabidopsis. Plant Cell 22(11): 3574-3588.
  41. Mitsuda, N., Ikeda, M., Takada, S., Takiguchi, Y., Kondou, Y., Yoshizumi, T., Fujita, M., Shinozaki, K., Matsui, M. and Ohme-Takagi, M. (2010). Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana. Plant Cell Physiol 51(12): 2145-2151.
  42. Koyama, T., Sato, F. and Ohme-Takagi, M. (2010). A role of TCP1 in the longitudinal elongation of leaves in Arabidopsis. Biosci Biotechnol Biochem 74(10): 2145-2147.
  43. Tejedor-Cano, J., Prieto-Dapena, P., Almoguera, C., Carranco, R., Hiratsu, K., Ohme-Takagi, M. and Jordano, J. (2010). Loss of function of the HSFA9 seed longevity program. Plant Cell Environ 33(8): 1408-1417.
  44. Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H. and Demura, T. (2010). VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis. Plant Cell 22(4): 1249-1263.
  45. Eklund, D. M., Staldal, V., Valsecchi, I., Cierlik, I., Eriksson, C., Hiratsu, K., Ohme-Takagi, M., Sundstrom, J. F., Thelander, M., Ezcurra, I. and Sundberg, E. (2010). The Arabidopsis thaliana STYLISH1 protein acts as a transcriptional activator regulating auxin biosynthesis. Plant Cell 22(2): 349-363.
  46. Matsui, K. and Ohme-Takagi, M. (2010). Detection of protein-protein interactions in plants using the transrepressive activity of the EAR motif repression domain. Plant J 61(4): 570-578.
  47. Shikata, M., Koyama, T., Mitsuda, N. and Ohme-Takagi, M. (2009). Arabidopsis SBP-box genes SPL10, SPL11 and SPL2 control morphological change in association with shoot maturation in the reproductive phase. Plant Cell Physiol 50(12): 2133-2145.
  48. Ikeda, M., Mitsuda, N. and Ohme-Takagi, M. (2009). Arabidopsis WUSCHEL is a bifunctional transcription factor that acts as a repressor in stem cell regulation and as an activator in floral patterning. Plant Cell 21(11): 3493-3505.
  49. Iwase, A., Hideno, A., Watanabe, K., Mitsuda, N. and Ohme-Takagi, M. (2009). A chimeric NST repressor has the potential to improve glucose productivity from plant cell walls. J Biotechnol 142(3-4): 279-284.
  50. Iwase, A., Matsui, K. and Ohme-Takagi, M. (2009). Manipulation of plant metabolism by transcription factors. Plant Biotechnology 26:29-38. (Review)
  51. Ikeda, M. and Ohme-Takagi, M. (2009). A novel group of transcriptional repressors in Arabidopsis. Plant Cell Physiol 50(5): 970-975.
  52. Mitsuda, N. and Ohme-Takagi, M. (2009). Functional analysis of transcription factors in Arabidopsis. Plant Cell Physiol 50(7): 1232-1248.
  53. Mitsuda, N. and Ohme-Takagi, M. (2008). NAC transcription factors NST1 and NST3 regulate pod shattering in a partially redundant manner by promoting secondary wall formation after the establishment of tissue identity. Plant J 56(5): 768-778.
  54. Kunieda, T., Mitsuda, N., Ohme-Takagi, M., Takeda, S., Aida, M., Tasaka, M., Kondo, M., Nishimura, M. and Hara-Nishimura, I. (2008). NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis. Plant Cell 20(10): 2631-2642.
  55. Matsui, K., Umemura, Y. and Ohme-Takagi, M. (2008). AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis. Plant J 55(6): 954-967.
  56. Shikata, M. and Ohme-Takagi, M. (2008). The utility of transcription factors for manipulation of floral traits. Plant Biotechnology 25 31-36. (Review)
  57. Mitsuda, N., Umemura, Y., Ikeda, M., Shikata, M., Koyama, T., Matsui, K., Narumi, T., Aida, R., Sasaki, K. and Hiyama, T. (2008). FioreDB: a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants. Plant biotechnology 25(1): 37-43.
  58. Narumi, T., Aida, R., Niki, T., Nishijima, T., Mitsuda, N., Hiratsu, K., Ohme-Takagi, M. and Ohtsubo, N. (2008). Chimeric AGAMOUS repressor induces serrated petal phenotype in Torenia fournieri similar to that induced by cytokinin application. PLANT BIOTECHNOLOGY-TOKYO- 25(1): 45.
Protocols by Masaru Ohme-Takagi
  1. Electrophoresis Mobility Shift Assay
  2. Quantification of Anthocyanin Content