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Jian-Guo Zhang


PhD in Biochemistry, The University of Melbourne/Ludwig Institute for Cancer Research, 1993

Current position

Senior Research Scientist, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

Publications (since 2005)

  1. Zhang, J. G.*, Czabotar, P. E.*, Policheni, A. N., Caminschi, I., Wan, S. S., Kitsoulis, S., Tullett, K. M., Robin, A. Y., Brammananth, R., van Delft, M. F., Lu, J., O'Reilly, L. A., Josefsson, E. C., Kile, B. T., Chin, W. J., Mintern, J. D., Olshina, M. A., Wong, W., Baum, J., Wright, M. D., Huang, D. C., Mohandas, N., Coppel, R. L., Colman, P. M., Nicola, N. A., Shortman, K. and Lahoud, M. H. (2012). The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments. Immunity 36(4): 646-657.
  2. Alari-Pahissa, E., Vega-Ramos, J., Zhang, J. G., Castano, A. R., Turley, S. J., Villadangos, J. A. and Lauzurica, P. (2012). Differential effect of CD69 targeting on bystander and antigen-specific T cell proliferation. J Leukoc Biol 92(1): 145-158.
  3. Li, R., Zhang, J. G., Churchill, J., Sourial, M., Southwell, B. R. and Hutson, J. M. (2012). Is matrix metalloproteinase required in postnatal testicular tubules for germ cell maturation? J Pediatr Surg 47(9): 1724-1729.
  4. Zhang, J. G. and Nicholson, S. E. (2013). Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and Western blot analysis. Methods Mol Biol 967: 249-259.
  5. Croker, B. A., O'Donnell, J. A., Nowell, C. J., Metcalf, D., Dewson, G., Campbell, K. J., Rogers, K. L., Hu, Y., Smyth, G. K., Zhang, J. G., White, M., Lackovic, K., Cengia, L. H., O'Reilly, L. A., Bouillet, P., Cory, S., Strasser, A. and Roberts, A. W. (2011). Fas-mediated neutrophil apoptosis is accelerated by Bid, Bak, and Bax and inhibited by Bcl-2 and Mcl-1. Proc Natl Acad Sci U S A 108(32): 13135-13140.
  6. Menkhorst, E., Zhang, J. G., Sims, N. A., Morgan, P. O., Soo, P., Poulton, I. J., Metcalf, D., Alexandrou, E., Gresle, M., Salamonsen, L. A., Butzkueven, H., Nicola, N. A. and Dimitriadis, E. (2011). Vaginally administered PEGylated LIF antagonist blocked embryo implantation and eliminated non-target effects on bone in mice. PLoS One 6(5): e19665.
  7. Croker, B. A., Lewis, R. S., Babon, J. J., Mintern, J. D., Jenne, D. E., Metcalf, D., Zhang, J. G., Cengia, L. H., O'Donnell, J. A. and Roberts, A. W. (2011). Neutrophils require SHP1 to regulate IL-1beta production and prevent inflammatory skin disease. J Immunol 186(2): 1131-1139.
  8. Burt, R. A., Watkins, L., Tan, I. K., Wang, N., Quirk, F., Mackin, L., Morgan, P., Zhang, J. G., Berzins, S. P., Morahan, G. and Brodnicki, T. C. (2010). An NZW-derived interval on chromosome 7 moderates sialadenitis, but not insulitis in congenic nonobese diabetic mice. J Immunol 184(2): 859-868.
  9. Walker, E. C., McGregor, N. E., Poulton, I. J., Solano, M., Pompolo, S., Fernandes, T. J., Constable, M. J., Nicholson, G. C., Zhang, J. G., Nicola, N. A., Gillespie, M. T., Martin, T. J. and Sims, N. A. (2010). Oncostatin M promotes bone formation independently of resorption when signaling through leukemia inhibitory factor receptor in mice. J Clin Invest 120(2): 582-592.
  10. Xu, Y.*, Kershaw, N. J.*, Luo, C. S., Soo, P., Pocock, M. J., Czabotar, P. E., Hilton, D. J., Nicola, N. A., Garrett, T. P.# and Zhang, J. G.# (2010). Crystal structure of the entire ectodomain of gp130: insights into the molecular assembly of the tall cytokine receptor complexes. J Biol Chem 285(28): 21214-21218.
  11. Stevenson, W. S., Hyland, C. D., Zhang, J. G., Morgan, P. O., Willson, T. A., Gill, A., Hilton, A. A., Viney, E. M., Bahlo, M., Masters, S. L., Hennebry, S., Richardson, S. J., Nicola, N. A., Metcalf, D., Hilton, D. J., Roberts, A. W. and Alexander, W. S. (2010). Deficiency of 5-hydroxyisourate hydrolase causes hepatomegaly and hepatocellular carcinoma in mice. Proc Natl Acad Sci U S A 107(38): 16625-16630.
  12. Boyle, K., Zhang, J. G., Nicholson, S. E., Trounson, E., Babon, J. J., McManus, E. J., Nicola, N. A. and Robb, L. (2009). Deletion of the SOCS box of suppressor of cytokine signaling 3 (SOCS3) in embryonic stem cells reveals SOCS box-dependent regulation of JAK but not STAT phosphorylation. Cell Signal 21(3): 394-404.
  13. Babon, J. J., Sabo, J. K., Zhang, J. G., Nicola, N. A. and Norton, R. S. (2009). The SOCS box encodes a hierarchy of affinities for Cullin5: implications for ubiquitin ligase formation and cytokine signalling suppression. J Mol Biol 387(1): 162-174.
  14. LA, O. R., Tai, L., Lee, L., Kruse, E. A., Grabow, S., Fairlie, W. D., Haynes, N. M., Tarlinton, D. M., Zhang, J. G., Belz, G. T., Smyth, M. J., Bouillet, P., Robb, L. and Strasser, A. (2009). Membrane-bound Fas ligand only is essential for Fas-induced apoptosis. Nature 461(7264): 659-663.
  15. Babon, J. J., Sabo, J. K., Soetopo, A., Yao, S., Bailey, M. F., Zhang, J. G., Nicola, N. A. and Norton, R. S. (2008). The SOCS box domain of SOCS3: structure and interaction with the elonginBC-cullin5 ubiquitin ligase. J Mol Biol 381(4): 928-940.
  16. Debrincat, M. A., Zhang, J. G., Willson, T. A., Silke, J., Connolly, L. M., Simpson, R. J., Alexander, W. S., Nicola, N. A., Kile, B. T. and Hilton, D. J. (2007). Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. J Biol Chem 282(7): 4728-4737.
  17. Kile, B. T., Panopoulos, A. D., Stirzaker, R. A., Hacking, D. F., Tahtamouni, L. H., Willson, T. A., Mielke, L. A., Henley, K. J., Zhang, J. G., Wicks, I. P., Stevenson, W. S., Nurden, P., Watowich, S. S. and Justice, M. J. (2007). Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood 110(7): 2371-2380.
  18. Greig, K. T., Antonchuk, J., Metcalf, D., Morgan, P. O., Krebs, D. L., Zhang, J. G., Hacking, D. F., Bode, L., Robb, L., Kranz, C., de Graaf, C., Bahlo, M., Nicola, N. A., Nutt, S. L., Freeze, H. H., Alexander, W. S., Hilton, D. J. and Kile, B. T. (2007). Agm1/Pgm3-mediated sugar nucleotide synthesis is essential for hematopoiesis and development. Mol Cell Biol 27(16): 5849-5859.
  19. Huyton, T.*, Zhang, J. G.*, Luo, C. S., Lou, M. Z., Hilton, D. J., Nicola, N. A. and Garrett, T. P. (2007). An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor. Proc Natl Acad Sci U S A 104(31): 12737-12742.
  20. White, C. A., Zhang, J. G., Salamonsen, L. A., Baca, M., Fairlie, W. D., Metcalf, D., Nicola, N. A., Robb, L. and Dimitriadis, E. (2007). Blocking LIF action in the uterus by using a PEGylated antagonist prevents implantation: a nonhormonal contraceptive strategy. Proc Natl Acad Sci U S A 104(49): 19357-19362.
  21. Masters, S. L., Yao, S., Willson, T. A., Zhang, J. G., Palmer, K. R., Smith, B. J., Babon, J. J., Nicola, N. A., Norton, R. S. and Nicholson, S. E. (2006). The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues. Nat Struct Mol Biol 13(1): 77-84.
  22. Wormald, S., Zhang, J. G., Krebs, D. L., Mielke, L. A., Silver, J., Alexander, W. S., Speed, T. P., Nicola, N. A. and Hilton, D. J. (2006). The comparative roles of suppressor of cytokine signaling-1 and -3 in the inhibition and desensitization of cytokine signaling. J Biol Chem 281(16): 11135-11143.
  23. Emery, B., Cate, H. S., Marriott, M., Merson, T., Binder, M. D., Snell, C., Soo, P. Y., Murray, S., Croker, B., Zhang, J. G., Alexander, W. S., Cooper, H., Butzkueven, H. and Kilpatrick, T. J. (2006). Suppressor of cytokine signaling 3 limits protection of leukemia inhibitory factor receptor signaling against central demyelination. Proc Natl Acad Sci U S A 103(20): 7859-7864.
  24. Smith, B. J., Huyton, T., Joosten, R. P., McKimm-Breschkin, J. L., Zhang, J. G., Luo, C. S., Lou, M. Z., Labrou, N. E. and Garrett, T. P. (2006). Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding. Acta Crystallogr D Biol Crystallogr 62(Pt 9): 947-952.
  25. Yao, S., Liu, M. S., Masters, S. L., Zhang, J. G., Babon, J. J., Nicola, N. A., Nicholson, S. E. and Norton, R. S. (2006). Dynamics of the SPRY domain-containing SOCS box protein 2: flexibility of key functional loops. Protein Sci 15(12): 2761-2772.
  26. Alexander, W. S., Viney, E. M., Zhang, J. G., Metcalf, D., Kauppi, M., Hyland, C. D., Carpinelli, M. R., Stevenson, W., Croker, B. A., Hilton, A. A., Ellis, S., Selan, C., Nandurkar, H. H., Goodnow, C. C., Kile, B. T., Nicola, N. A., Roberts, A. W. and Hilton, D. J. (2006). Thrombocytopenia and kidney disease in mice with a mutation in the C1galt1 gene. Proc Natl Acad Sci U S A 103(44): 16442-16447.
  27. Nicholson, S. E., Metcalf, D., Sprigg, N. S., Columbus, R., Walker, F., Silva, A., Cary, D., Willson, T. A., Zhang, J. G., Hilton, D. J., Alexander, W. S. and Nicola, N. A. (2005). Suppressor of cytokine signaling (SOCS)-5 is a potential negative regulator of epidermal growth factor signaling. Proc Natl Acad Sci U S A 102(7): 2328-2333.
  28. Yao, S., Masters, S. L., Zhang, J. G., Palmer, K. R., Babon, J. J., Nicola, N. A., Nicholson, S. E. and Norton, R. S. (2005). Backbone 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2). J Biomol NMR 31(1): 69-70.
  29. Fenner, J. E., Starr, R., Cornish, A. L., Zhang, J. G., Metcalf, D., Schreiber, R. D., Sheehan, K., Hilton, D. J., Alexander, W. S. and Hertzog, P. J. (2006). Suppressor of cytokine signaling 1 regulates the immune response to infection by a unique inhibition of type I interferon activity. Nat Immunol 7(1): 33-39.
Protocols by Jian-Guo Zhang
  1. Co-sedimentation Assay for the Detection of Direct Binding to F-actin
  2. Determination of Pseudokinase-ligand Interaction by a Fluorescence-based Thermal Shift Assay