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Leonid Margolis


Ph.D. in Oncology, Moscow State University, Russia, 1974
Dr. Sci (advanced degree) in Biophysics, Cell Biology, Moscow State University, Russia, 1985

Current position

Active Deputy Director, NASA/NIH Center for Three-Dimensional Tissue Culture, 1995
Active Senior Investigator, NICHD, NIH, 2000
Active Chief, Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH, 2001


Selected peer-reviewed publications (out of 213 total)

  1. Introini, A., Vanpouille, C., Lisco, A., Grivel, J. C. and Margolis, L. (2013). Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo. PLoS Pathog 9(2): e1003148.
  2. Andrei, G., Lisco, A., Vanpouille, C., Introini, A., Balestra, E., van den Oord, J., Cihlar, T., Perno, C. F., Snoeck, R., Margolis, L. and Balzarini, J. (2011). Topical tenofovir, a microbicide effective against HIV, inhibits herpes simplex virus-2 replication. Cell Host Microbe 10(4): 379-389.
  3. Saba, E., Grivel, J. C., Vanpouille, C., Brichacek, B., Fitzgerald, W., Margolis, L. and Lisco, A. (2010). HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model. Mucosal Immunol 3(3): 280-290.
  4. Lisco, A., Vanpouille, C. and Margolis, L. (2009). War and peace between microbes: HIV-1 interactions with coinfecting viruses. Cell Host Microbe 6(5): 403-408.
  5. Grivel, J. C. and Margolis, L. (2009). Use of human tissue explants to study human infectious agents. Nat Protoc 4(2): 256-269.
  6. Lisco, A., Vanpouille, C., Tchesnokov, E. P., Grivel, J. C., Biancotto, A., Brichacek, B., Elliott, J., Fromentin, E., Shattock, R., Anton, P., Gorelick, R., Balzarini, J., McGuigan, C., Derudas, M., Gotte, M., Schinazi, R. F. and Margolis, L. (2008). Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues. Cell Host Microbe 4(3): 260-270.
  7. Munch, J., Rucker, E., Standker, L., Adermann, K., Goffinet, C., Schindler, M., Wildum, S., Chinnadurai, R., Rajan, D., Specht, A., Gimenez-Gallego, G., Sanchez, P. C., Fowler, D. M., Koulov, A., Kelly, J. W., Mothes, W., Grivel, J. C., Margolis, L., Keppler, O. T., Forssmann, W. G. and Kirchhoff, F. (2007). Semen-derived amyloid fibrils drastically enhance HIV infection. Cell 131(6): 1059-1071.
  8. Margolis, L. and Shattock, R. (2006). Selective transmission of CCR5-utilizing HIV-1: the 'gatekeeper' problem resolved? Nat Rev Microbiol 4(4): 312-317.
  9. Margolis, L. (2003). Cytokines--strategic weapons in germ warfare? Nat Biotechnol 21(1): 15-16.
  10. Grivel, J. C., Ito, Y., Faga, G., Santoro, F., Shaheen, F., Malnati, M. S., Fitzgerald, W., Lusso, P. and Margolis, L. (2001). Suppression of CCR5- but not CXCR4-tropic HIV-1 in lymphoid tissue by human herpesvirus 6. Nat Med 7(11): 1232-1235.
  11. Malkevich, N., Womack, C., Pandya, P., Grivel, J. C., Fauci, A. S. and Margolis, L. (2001). Human immunodeficiency virus type 1 (HIV-1) non-B subtypes are similar to HIV-1 subtype B in that coreceptor specificity is a determinant of cytopathicity in human lymphoid tissue infected ex vivo. J Virol 75(21): 10520-10522.
  12. Glushakova, S., Yi, Y., Grivel, J. C., Singh, A., Schols, D., De Clercq, E., Collman, R. G. and Margolis, L. (1999). Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo. J Clin Invest 104(5): R7-R11.
  13. Grivel, J. C. and Margolis, L. B. (1999). CCR5- and CXCR4-tropic HIV-1 are equally cytopathic for their T-cell targets in human lymphoid tissue. Nat Med 5(3): 344-346.
  14. Penn, M. L., Grivel, J. C., Schramm, B., Goldsmith, M. A. and Margolis, L. (1999). CXCR4 utilization is sufficient to trigger CD4+ T cell depletion in HIV-1-infected human lymphoid tissue. Proc Natl Acad Sci U S A 96(2): 663-668.
  15. Glushakova, S., Grivel, J. C., Fitzgerald, W., Sylwester, A., Zimmerberg, J. and Margolis, L. B. (1998). Evidence for the HIV-1 phenotype switch as a causal factor in acquired immunodeficiency. Nat Med 4(3): 346-349.
  16. Margolis, L. B., Glushakova, S., Grivel, J. C. and Murphy, P. M. (1998). Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines. J Clin Invest 101(9): 1876-1880.
  17. Glushakova, S., Baibakov, B., Margolis, L. B. and Zimmerberg, J. (1995). Infection of human tonsil histocultures: a model for HIV pathogenesis. Nat Med 1(12): 1320-1322.
  18. Margolis, L. B., Tikhonov, A. N. and Vasilieva, E. Y. (1980). Platelet adhesion to fluid and solid phospholipid membranes. Cell 19(1): 189-195.
  19. Margolis, L. B., Vasilieva, E. J., Vasiliev, J. M. and Gelfand, I. M. (1979). Upper surfaces of epithelial sheets and of fluid lipid films are nonadhesive for platelets. Proc Natl Acad Sci U S A 76(5): 2303-2305.
  20. Ivanova, O. Y. and Margolis, L. B. (1973). The use of phospholipid film for shaping cell cultures. Nature 242(5394): 200-201.
Protocols by Leonid Margolis
  1. An ex vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue