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Christophe Vanpouille


Ph.D. in Cellular biology/Biochemistry, University of Sciences and Technologies of Lille, France, 2003

Current position

Staff Scientist, Section of Intercellular Interactions, Program in Physical Biology, NICHD, NIH


Original contributions in peer-review journals

  1. McGuigan, C., Bourdin, C., Derudas, M., Hamon, N., Hinsinger, K., Kandil, S., Madela, K., Meneghesso, S., Pertusati, F., Serpi, M., Slusarczyk, M., Chamberlain, S., Kolykhalov, A., Vernachio, J., Vanpouille, C., Introini, A., Margolis, L. and Balzarini, J. (2013). Design, synthesis and biological evaluation of phosphorodiamidate prodrugs of antiviral and anticancer nucleosides. Eur J Med Chem 70: 326-340.
  2. Arakelyan, A., Fitzgerald, W., Grivel, J. C., Vanpouille, C. and Margolis, L. (2014). Histocultures (tissue explants) in human retrovirology. Methods Mol Biol 1087: 233-248.
  3. Balzarini, J., Andrei, G., Balestra, E., Huskens, D., Vanpouille, C., Introini, A., Zicari, S., Liekens, S., Snoeck, R., Holy, A., Perno, C. F., Margolis, L. and Schols, D. (2013). A multi-targeted drug candidate with dual anti-HIV and anti-HSV activity. PLoS Pathog 9(7): e1003456.
  4. Introini, A., Vanpouille, C., Lisco, A., Grivel, J. C. and Margolis, L. (2013). Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo. PLoS Pathog 9(2): e1003148.
  5. Lisco, A., Introini, A., Munawwar, A., Vanpouille, C., Grivel, J. C., Blank, P., Singh, S. and Margolis, L. (2012). HIV-1 imposes rigidity on blood and semen cytokine networks. Am J Reprod Immunol 68(6): 515-521.
  6. Vanpouille, C., Arakelyan, A. and Margolis, L. (2012). Microbicides: still a long road to success. Trends Microbiol 20(8): 369-375.
  7. Vanpouille, C., Lisco, A., Introini, A., Grivel, J. C., Munawwar, A., Merbah, M., Schinazi, R. F., Derudas, M., McGuigan, C., Balzarini, J. and Margolis, L. (2012). Exploiting the anti-HIV-1 activity of acyclovir: suppression of primary and drug-resistant HIV isolates and potentiation of the activity by ribavirin. Antimicrob Agents Chemother 56(5): 2604-2611.
  8. Lisco, A., Munawwar, A., Introini, A., Vanpouille, C., Saba, E., Feng, X., Grivel, J. C., Singh, S. and Margolis, L. (2012). Semen of HIV-1-infected individuals: local shedding of herpesviruses and reprogrammed cytokine network. J Infect Dis 205(1): 97-105.
  9. Andrei, G., Lisco, A., Vanpouille, C., Introini, A., Balestra, E., van den Oord, J., Cihlar, T., Perno, C. F., Snoeck, R., Margolis, L. and Balzarini, J. (2011). Topical tenofovir, a microbicide effective against HIV, inhibits herpes simplex virus-2 replication. Cell Host Microbe 10(4): 379-389.
  10. Merbah, M., Introini, A., Fitzgerald, W., Grivel, J. C., Lisco, A., Vanpouille, C. and Margolis, L. (2011). Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am J Reprod Immunol 65(3): 268-278.
  11. Brichacek, B., Vanpouille, C., Kiselyeva, Y., Biancotto, A., Merbah, M., Hirsch, I., Lisco, A., Grivel, J. C. and Margolis, L. (2010). Contrasting roles for TLR ligands in HIV-1 pathogenesis. PLoS One 5(9).
  12. Saba, E., Grivel, J. C., Vanpouille, C., Brichacek, B., Fitzgerald, W., Margolis, L. and Lisco, A. (2010). HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model. Mucosal Immunol 3(3): 280-290.
  13. Vanpouille, C., Lisco, A., Derudas, M., Saba, E., Grivel, J. C., Brichacek, B., Scrimieri, F., Schinazi, R., Schols, D., McGuigan, C., Balzarini, J. and Margolis, L. (2010). A new class of dual-targeted antivirals: monophosphorylated acyclovir prodrug derivatives suppress both human immunodeficiency virus type 1 and herpes simplex virus type 2. J Infect Dis 201(4): 635-643.
  14. Lisco, A., Vanpouille, C. and Margolis, L. (2009). War and peace between microbes: HIV-1 interactions with coinfecting viruses. Cell Host Microbe 6(5): 403-408.
  15. Vanpouille, C., Lisco, A. and Margolis, L. (2009). Acyclovir: a new use for an old drug. Curr Opin Infect Dis 22(6): 583-587.
  16. Lisco, A., Vanpouille, C. and Margolis, L. (2009). A missed point in deciphering the viral synergy between herpes simplex virus and HIV. Lancet Infect Dis 9(9): 522-523.
  17. Derudas, M., Carta, D., Brancale, A., Vanpouille, C., Lisco, A., Margolis, L., Balzarini, J. and McGuigan, C. (2009). The application of phosphoramidate protide technology to acyclovir confers anti-HIV inhibition. J Med Chem 52(17): 5520-5530.
  18. Biancotto, A., Grivel, J. C., Lisco, A., Vanpouille, C., Markham, P. D., Gallo, R. C., Margolis, L. B. and Lusso, P. (2009). Evolution of SIV toward RANTES resistance in macaques rapidly progressing to AIDS upon coinfection with HHV-6A. Retrovirology 6: 61.
  19. Tchesnokov, E. P., Obikhod, A., Massud, I., Lisco, A., Vanpouille, C., Brichacek, B., Balzarini, J., McGuigan, C., Derudas, M., Margolis, L., Schinazi, R. F. and Gotte, M. (2009). Mechanisms associated with HIV-1 resistance to acyclovir by the V75I mutation in reverse transcriptase. J Biol Chem 284(32): 21496-21504.
  20. Vanpouille, C., Lisco, A. and Margolis, L. (2009). Off-label indication: acyclovir as an anti-HIV drug? Future virology 4(1): 1-5.
  21. Lisco, A., Vanpouille, C. and Margolis, L. (2009). Coinfecting viruses as determinants of HIV disease. Curr HIV/AIDS Rep 6(1): 5-12.
  22. Biancotto, A., Brichacek, B., Chen, S. S., Fitzgerald, W., Lisco, A., Vanpouille, C., Margolis, L. and Grivel, J. C. (2009). A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24. J Virol Methods 157(1): 98-101.
  23. Lisco, A.*, Vanpouille, C.*, Tchesnokov, E. P., Grivel, J. C., Biancotto, A., Brichacek, B., Elliott, J., Fromentin, E., Shattock, R., Anton, P., Gorelick, R., Balzarini, J., McGuigan, C., Derudas, M., Gotte, M., Schinazi, R. F. and Margolis, L. (2008). Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues. Cell Host Microbe 4(3): 260-270. (*authors contributed equally).
  24. Lisco, A. and Vanpouille, C. (2008). HSV-2 suppression and the incidence of HIV. N Engl J Med 359(5): 535; author reply 535.
  25. Biancotto, A., Iglehart, S. J., Lisco, A., Vanpouille, C., Grivel, J. C., Lurain, N. S., Reichelderfer, P. S. and Margolis, L. B. (2008). Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses 24(3): 453-462.
  26. Biancotto, A., Iglehart, S. J., Vanpouille, C., Condack, C. E., Lisco, A., Ruecker, E., Hirsch, I., Margolis, L. B. and Grivel, J. C. (2008). HIV-1 induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. Blood 111(2): 699-704.
  27. Vanpouille, C., Biancotto, A., Lisco, A. and Brichacek, B. (2007). Interactions between human immunodeficiency virus type 1 and vaccinia virus in human lymphoid tissue ex vivo. J Virol 81(22): 12458-12464.
  28. Vanpouille, C., Deligny, A., Delehedde, M., Denys, A., Melchior, A., Lienard, X., Lyon, M., Mazurier, J., Fernig, D. G. and Allain, F. (2007). The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine residue. J Biol Chem 282(33): 24416-24429.
  29. Biancotto, A., Grivel, J. C., Iglehart, S. J., Vanpouille, C., Lisco, A., Sieg, S. F., Debernardo, R., Garate, K., Rodriguez, B., Margolis, L. B. and Lederman, M. M. (2007). Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood 109(10): 4272-4279.
  30. Pakula, R., Melchior, A., Denys, A., Vanpouille, C., Mazurier, J. and Allain, F. (2007). Syndecan-1/CD147 association is essential for cyclophilin B-induced activation of p44/42 mitogen-activated protein kinases and promotion of cell adhesion and chemotaxis. Glycobiology 17(5): 492-503.
  31. Lisco, A., Grivel, J. C., Biancotto, A., Vanpouille, C., Origgi, F., Malnati, M. S., Schols, D., Lusso, P. and Margolis, L. B. (2007). Viral interactions in human lymphoid tissue: Human herpesvirus 7 suppresses the replication of CCR5-tropic human immunodeficiency virus type 1 via CD4 modulation. J Virol 81(2): 708-717.
  32. Gwinn, W. M., Damsker, J. M., Falahati, R., Okwumabua, I., Kelly-Welch, A., Keegan, A. D., Vanpouille, C., Lee, J. J., Dent, L. A., Leitenberg, D., Bukrinsky, M. I. and Constant, S. L. (2006). Novel approach to inhibit asthma-mediated lung inflammation using anti-CD147 intervention. J Immunol 177(7): 4870-4879.
  33. Brichacek, B., Vanpouille, C., Trachtenberg, A. J., Pushkarsky, T., Dubrovsky, L., Martin, G., Simon, G. and Bukrinsky, M. (2006). Long-term changes of serum chemokine levels in vaccinated military personnel. BMC Immunol 7: 21.
  34. Haffar, O., Dubrovsky, L., Lowe, R., Berro, R., Kashanchi, F., Godden, J., Vanpouille, C., Bajorath, J. and Bukrinsky, M. (2005). Oxadiazols: a new class of rationally designed anti-human immunodeficiency virus compounds targeting the nuclear localization signal of the viral matrix protein. J Virol 79(20): 13028-13036.
  35. Pushkarsky, T., Yurchenko, V., Vanpouille, C., Brichacek, B., Vaisman, I., Hatakeyama, S., Nakayama, K. I., Sherry, B. and Bukrinsky, M. I. (2005). Cell surface expression of CD147/EMMPRIN is regulated by cyclophilin 60. J Biol Chem 280(30): 27866-27871.
  36. West, D. C., Rees, C. G., Duchesne, L., Patey, S. J., Terry, C. J., Turnbull, J. E., Delehedde, M., Heegaard, C. W., Allain, F., Vanpouille, C., Ron, D. and Fernig, D. G. (2005). Interactions of multiple heparin binding growth factors with neuropilin-1 and potentiation of the activity of fibroblast growth factor-2. J Biol Chem 280(14): 13457-13464.
  37. Vanpouille, C., Denys, A., Carpentier, M., Pakula, R., Mazurier, J. and Allain, F. (2004). Octasaccharide is the minimal length unit required for efficient binding of cyclophilin B to heparin and cell surface heparan sulphate. Biochem J 382(Pt 2): 733-740.
  38. Allain, F., Vanpouille, C., Denys, A., Pakula, R., Carpentier, M. and Mazurier, J. (2003). Involvement of GAGs in the activity of pro-inflammatory factors. Adv Exp Med Biol 535: 95-106.
  39. Carpentier, M., Allain, F., Slomianny, M. C., Durieux, S., Vanpouille, C., Haendler, B. and Spik, G. (2002). Receptor type I and type II binding regions and the peptidyl-prolyl isomerase site of cyclophilin B are required for enhancement of T-lymphocyte adhesion to fibronectin. Biochemistry 41(16): 5222-5229.
  40. Allain, F., Vanpouille, C., Carpentier, M., Slomianny, M. C., Durieux, S. and Spik, G. (2002). Interaction with glycosaminoglycans is required for cyclophilin B to trigger integrin-mediated adhesion of peripheral blood T lymphocytes to extracellular matrix. Proc Natl Acad Sci U S A 99(5): 2714-2719.

Chapters & reviews (peer-reviewed)

  1. Introini, A., Vanpouille, C., Grivel, J.C. and Margolis, L. (2014). An ex vivo model of HIV-1 infection in human lymphoid tissue and cervico-vaginal tissue. Bio-Protocol 4(4): http://www.bio-protocol.org/wenzhang.aspx?id=1047.
  2. Delehedde, M., Sarrazin, S., Adam, E., Motte, V. and Vanpouille, C. (2006). Proteoglycans and glycosaminoglycans: complex molecules with modulating activity. In "New developments in Therapeutic Glycomics". Eds. Maryse Delehedde and Hugues Lortat-Jacob. pp 1-13.
  3. Lassalle, P., Adam, E., Sarrazin, S., Depontieu, F., Motte, V., Lequien, S., Grigoriu, B., De Freitas-Carreras, N., Vanpouille, C. and Delehedde, M. (2006). Proteoglycans and glycosaminoglycans: key players in inflammation. In "New developments in Therapeutic Glycomics". Eds. Maryse Delehedde and Hugues Lortat-Jacob. pp 15-31.
  4. Delehedde, M., Allain, F., Payne, S.,J., Borgo, R., Vanpouille, C., Fernig, D. G. and Deudon, E. (2002). Proteoglycans in inflammation. Curr. Med. Chem. - Anti-Inflammatory & Anti-Allergy Agents 1, 89-102.
Protocols by Christophe Vanpouille
  1. An ex vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue