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Takafumi Yokota

Education

Ph.D. in Medical science, Osaka University Graduate School of Medicine, 2000

Current position

Assistant Professor, Department of Hematology and Oncology, Osaka University Graduate School of Medicine

Publications

Papers as the first author

  1. Yokota, T., Sudo, T., Ishibashi, T., Doi, Y., Ichii, M., Orirani, K. and Kanakura, Y. (2013). Complementary regulation of early B-lymphoid differentiation by genetic and epigenetic mechanisms. Int J Hematol 98(4): 382-389.
  2. Satoh, Y., Yokota, T., Sudo, T., Kondo, M., Lai, A., Kincade, P. W., Kouro, T., Iida, R., Kokame, K., Miyata, T., Habuchi, Y., Matsui, K., Tanaka, H., Matsumura, I., Oritani, K., Kohwi-Shigematsu, T. and Kanakura, Y. (2013). The Satb1 protein directs hematopoietic stem cell differentiation toward lymphoid lineages. Immunity 38(6): 1105-1115.
  3. Nishida, Y., Saito, Y., Yokota, T., Kanda, T. and Mizusawa, H. (2009). Skeletal muscle MRI in complex regional pain syndrome. Intern Med 48(4): 209-212.
  4. Yokota, T., Oritani, K., Butz, S., Kokame, K., Kincade, P. W., Miyata, T., Vestweber, D. and Kanakura, Y. (2009). The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice. Blood 113(13): 2914-2923.
  5. Yokota, T., Oritani, K., Garrett, K. P., Kouro, T., Nishida, M., Takahashi, I., Ichii, M., Satoh, Y., Kincade, P. W. and Kanakura, Y. (2008). Soluble frizzled-related protein 1 is estrogen inducible in bone marrow stromal cells and suppresses the earliest events in lymphopoiesis. J Immunol 181(9): 6061-6072.
  6. Yokota, T., Huang, J., Tavian, M., Nagai, Y., Hirose, J., Zuniga-Pflucker, J. C., Peault, B. and Kincade, P. W. (2006). Tracing the first waves of lymphopoiesis in mice. Development 133(10): 2041-2051.
  7. Yokota, T., Kawakami, Y., Nagai, Y., Ma, J. X., Tsai, J. Y., Kincade, P. W. and Sato, S. (2006). Bone marrow lacks a transplantable progenitor for smooth muscle type alpha-actin-expressing cells. Stem Cells 24(1): 13-22.
  8. Yao, L., Yokota, T., Xia, L., Kincade, P. W. and McEver, R. P. (2005). Bone marrow dysfunction in mice lacking the cytokine receptor gp130 in endothelial cells. Blood 106(13): 4093-4101.
  9. Yokota, T. and Igarashi, H. (2005). The origin of lymphopoietic cells in the ontogeny. Clinical Immunology (Japanese) 43:595-602.
  10. Yokota, T., Meka, C. S., Kouro, T., Medina, K. L., Igarashi, H., Takahashi, M., Oritani, K., Funahashi, T., Tomiyama, Y., Matsuzawa, Y. and Kincade, P. W. (2003). Adiponectin, a fat cell product, influences the earliest lymphocyte precursors in bone marrow cultures by activation of the cyclooxygenase-prostaglandin pathway in stromal cells. J Immunol 171(10): 5091-5099.
  11. Yokota, T., Kouro, T., Hirose, J., Igarashi, H., Garrett, K. P., Gregory, S. C., Sakaguchi, N., Owen, J. J. and Kincade, P. W. (2003). Unique properties of fetal lymphoid progenitors identified according to RAG1 gene expression. Immunity 19(3): 365-375.
  12. Rossi, M. I., Yokota, T., Medina, K. L., Garrett, K. P., Comp, P. C., Schipul, A. H., Jr. and Kincade, P. W. (2003). B lymphopoiesis is active throughout human life, but there are developmental age-related changes. Blood 101(2): 576-584.
  13. Yokota, T., Meka, C. S., Medina, K. L., Igarashi, H., Comp, P. C., Takahashi, M., Nishida, M., Oritani, K., Miyagawa, J., Funahashi, T., Tomiyama, Y., Matsuzawa, Y. and Kincade, P. W. (2002). Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins. J Clin Invest 109(10): 1303-1310.
  14. Yokota, T. (2000). Adiponectin and macrophage function. Clinical Immunology (Japanese) Nov. 34;5.
  15. Yokota, T., Takahashi, M. and Funahashi, T. (2000). Adipocytokines. Inflammation Immunology (Japanese) 8:624-629.
  16. Yokota, T., Oritani, K., Takahashi, I., Ishikawa, J., Matsuyama, A., Ouchi, N., Kihara, S., Funahashi, T., Tenner, A. J., Tomiyama, Y. and Matsuzawa, Y. (2000). Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood 96(5): 1723-1732.
  17. Yokota, T., Oritani, K., Mitsui, H., Aoyama, K., Ishikawa, J., Sugahara, H., Matsumura, I., Tsai, S., Tomiyama, Y., Kanakura, Y. and Matsuzawa, Y. (1998). Growth-supporting activities of fibronectin on hematopoietic stem/progenitor cells in vitro and in vivo: structural requirement for fibronectin activities of CS1 and cell-binding domains. Blood 91(9): 3263-3272.
  18. Yokota, T., Teshima, H., Okajima, Y., Tsuboi, A., Oji, Y., Karasuno, T., Hiraoka, A. and Masaoka, T. (1996). [Septicemia associated with hematopoietic disorders and its features according to respective primary disorders]. Kansenshogaku Zasshi 70(2): 108-115.
  19. Yokota, T., Tsuboi, A., Okajima, Y., Oji, Y., Moriyama, Y., Tatekawa, T., Karasuno, T., Tominanga, N., Tejima, H., Hiraoka, A. and et al. (1994). Treatment of graft failure after bone marrow transplantation. Leuk Res 18(11): 875-876.

Papers as a co-author

  1. Zhang, Q., Iida, R., Yokota, T. and Kincade, P. W. (2013). Early events in lymphopoiesis: an update. Curr Opin Hematol 20(4): 265-272.
  2. Sudo, T., Yokota, T., Oritani, K., Satoh, Y., Sugiyama, T., Ishida, T., Shibayama, H., Ezoe, S., Fujita, N., Tanaka, H., Maeda, T., Nagasawa, T. and Kanakura, Y. (2012). The endothelial antigen ESAM monitors hematopoietic stem cell status between quiescence and self-renewal. J Immunol 189(1): 200-210.
  3. Saitoh, N., Oritani, K., Saito, K., Yokota, T., Ichii, M., Sudo, T., Fujita, N., Nakajima, K., Okada, M. and Kanakura, Y. (2011). Identification of functional domains and novel binding partners of STIM proteins. J Cell Biochem 112(1): 147-156.
  4. Ichii, M., Oritani, K., Yokota, T., Zhang, Q., Garrett, K. P., Kanakura, Y. and Kincade, P. W. (2010). The density of CD10 corresponds to commitment and progression in the human B lymphoid lineage. PLoS One 5(9): e12954.
  5. Ichii, M., Oritani, K., Yokota, T., Schultz, D. C., Holter, J. L., Kanakura, Y. and Kincade, P. W. (2010). Stromal cell-free conditions favorable for human B lymphopoiesis in culture. J Immunol Methods 359(1-2): 47-55.
  6. Ichii, M., Oritani, K., Yokota, T., Nishida, M., Takahashi, I., Shirogane, T., Ezoe, S., Saitoh, N., Tanigawa, R., Kincade, P. W. and Kanakura, Y. (2008). Regulation of human B lymphopoiesis by the transforming growth factor-beta superfamily in a newly established coculture system using human mesenchymal stem cells as a supportive microenvironment. Exp Hematol 36(5): 587-597.
  7. Li, W., Ishihara, K., Yokota, T., Nakagawa, T., Koyama, N., Jin, J., Mizuno-Horikawa, Y., Wang, X., Miyoshi, E., Taniguchi, N. and Kondo, A. (2008). Reduced alpha4beta1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase deficient mice. Glycobiology 18(1): 114-124.
  8. Masaie, H., Oritani, K., Yokota, T., Takahashi, I., Shirogane, T., Ujiie, H., Ichii, M., Saitoh, N., Maeda, T., Tanigawa, R., Oka, K., Hoshida, Y., Tomiyama, Y. and Kanakura, Y. (2007). Adiponectin binds to chemokines via the globular head and modulates interactions between chemokines and heparan sulfates. Exp Hematol 35(6): 947-956.
  9. Baba, Y., Yokota, T., Spits, H., Garrett, K. P., Hayashi, S. and Kincade, P. W. (2006). Constitutively active beta-catenin promotes expansion of multipotent hematopoietic progenitors in culture. J Immunol 177(4): 2294-2303.
  10. Ujiie, H., Oritani, K., Kato, H., Yokota, T., Takahashi, I., Maeda, T., Masaie, H., Ichii, M., Kamada, Y., Tamura, S., Kihara, S., Funahashi, T., Tomiyama, Y. and Kanakura, Y. (2006). Identification of amino-terminal region of adiponectin as a physiologically functional domain. J Cell Biochem 98(1): 194-207.
  11. Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. (2005). Isolation of prolymphocytes from bone marrow and fetal liver. Curr Protoc Immunol Chapter 22: Unit 22F 21.
  12. Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. (2005). In vitro differentiation and measurement of B cell progenitor activity in culture. Curr Protoc Immunol Chapter 22: Unit 22F 22.
  13. Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. (2005). Measurement of natural killer cell progenitor activity in culture. Curr Protoc Immunol Chapter 22: Unit 22F 23.
  14. Pelayo, R., Welner, R., Perry, S. S., Huang, J., Baba, Y., Yokota, T. and Kincade, P. W. (2005). Lymphoid progenitors and primary routes to becoming cells of the immune system. Curr Opin Immunol 17(2): 100-107.
  15. Igarashi, H., Medina, K. L., Yokota, T., Rossi, M. I., Sakaguchi, N., Comp, P. C. and Kincade, P. W. (2005). Early lymphoid progenitors in mouse and man are highly sensitive to glucocorticoids. Int Immunol 17(5): 501-511.
  16. Kolar, G. R., Yokota, T., Rossi, M. I., Nath, S. K. and Capra, J. D. (2004). Human fetal, cord blood, and adult lymphocyte progenitors have similar potential for generating B cells with a diverse immunoglobulin repertoire. Blood 104(9): 2981-2987.
  17. Hirose, J., Kouro, T., Igarashi, H., Yokota, T., Sakaguchi, N. and Kincade, P. W. (2002). A developing picture of lymphopoiesis in bone marrow. Immunol Rev 189: 28-40.
  18. Kincade, P. W., Igarashi, H., Medina, K. L., Kouro, T., Yokota, T., Rossi, M. I., Owen, J. J., Garrett, K. P., Sun, X. H. and Sakaguchi, N. (2002). Lymphoid lineage cells in adult murine bone marrow diverge from those of other blood cells at an early, hormone-sensitive stage. Semin Immunol 14(6): 385-394.
  19. Kincade, P. W., Owen, J. J., Igarashi, H., Kouro, T., Yokota, T. and Rossi, M. I. (2002). Nature or nurture? Steady-state lymphocyte formation in adults does not recapitulate ontogeny. Immunol Rev 187: 116-125.
  20. Igarashi, H., Gregory, S. C., Yokota, T., Sakaguchi, N. and Kincade, P. W. (2002). Transcription from the RAG1 locus marks the earliest lymphocyte progenitors in bone marrow. Immunity 17(2): 117-130.
  21. Igarashi, H., Kouro, T., Yokota, T., Comp, P. C. and Kincade, P. W. (2001). Age and stage dependency of estrogen receptor expression by lymphocyte precursors. Proc Natl Acad Sci U S A 98(26): 15131-15136.
  22. Yoshida, H., Tomiyama, Y., Ishikawa, J., Oritani, K., Matsumura, I., Shiraga, M., Yokota, T., Okajima, Y., Ogawa, M., Miyagawa, J., Nishiura, T. and Matsuzawa, Y. (2000). Integrin-associated protein/CD47 regulates motile activity in human B-cell lines through CDC42. Blood 96(1): 234-241.
  23. Oritani, K., Medina, K. L., Tomiyama, Y., Ishikawa, J., Okajima, Y., Ogawa, M., Yokota, T., Aoyama, K., Takahashi, I., Kincade, P. W. and Matsuzawa, Y. (2000). Limitin: An interferon-like cytokine that preferentially influences B-lymphocyte precursors. Nat Med 6(6): 659-666.
  24. Aoyama, K., Oritani, K., Yokota, T., Ishikawa, J., Nishiura, T., Miyake, K., Kanakura, Y., Tomiyama, Y., Kincade, P. W. and Matsuzawa, Y. (1999). Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood 93(8): 2586-2594.
  25. Oritani, K., Tomiyama, Y., Kincade, P. W., Aoyama, K., Yokota, T., Matsumura, I., Kanakura, Y., Nakajima, K., Hirano, T. and Matsuzawa, Y. (1999). Both Stat3-activation and Stat3-independent BCL2 downregulation are important for interleukin-6-induced apoptosis of 1A9-M cells. Blood 93(4): 1346-1354.
  26. Yoshida, H., Nishiura, T., Karasuno, T., Matsumura, I., Ishikawa, J., Yoshimura, M., Yokota, T., Okajima, Y., Ogawa, M., Kanakura, Y., Tomiyama, Y. and Matsuzawa, Y. (1998). Effect of the interaction between fibronectin and VLA-4 on the proliferation of human B cells, especially a novel human B-cell line, OPM-3. Br J Haematol 103(3): 804-812.
  27. Oritani, K., Kanakura, Y., Aoyama, K., Yokota, T., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Tomiyama, Y., Matsuzawa, Y. and Kincade, P. W. (1997). Matrix glycoprotein SC1/ECM2 augments B lymphopoiesis. Blood 90(9): 3404-3413.
  28. Tatekawa, T., Yokota, T., Oji, Y., Moriyama, Y., Tominaga, N., Teshima, H., Hiraoka, A., Nakamura, H., Shibata, H., Minamishima, Y. and et al. (1994). [The monitoring of cytomegalovirus antigenemia and arterial oxygen saturation for the early detection of cytomegalovirus pneumonia]. Rinsho Ketsueki 35(11): 1261-1266.

Books

  1. Sudo, T., Yokota, T., Ishibashi, T., Ichii, M., Doi, Y., Oritani, K. and Kanakura, Y. (2013). Canonical HSC Markers and Recent Achievements. Stem Cell Biology in Normal Life and Diseases. Intech Open Access Publisher, Croatia, 51-64.
  2. Yokota, T., Oritani, K., Butz, S., Ewers, S., Vestweber, D. and Kanakura, Y. (2012). Markers for Hematopoietic Stem Cells: Histories and Recent Achievements. Advances in Hematopoietic Stem Cell Research. Intech Open Access Publisher, Croatia, 77-88. 
  3. Yokota, T., Oritani, K. and Kanakura, Y. (2010). ESAM; a new marker for hematopoietic stem cells. Annual Review Ketsueki (Japanese). Chugai-igakusha, Japan, 12-21.
  4. Yokota, T. (1996). Treatments Before and After Bone Marrow Transplantation. In: Toru Masaoka MD. The Chief of the 5th Department of Internal Medicine, Osaka Medical Center for Cancer and Cardiovascular Diseases. Leukemia; Struggle and Pleasure of Recovery (Japanese). Iyaku Journal Co.Ltd, 103-110.
Protocols by Takafumi Yokota
  1. Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors
  2. DNA PCR Assays for Igh Rearrangement