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Nobuyuki Matoba

Education

Ph.D. in Applied Life Science, Kyoto University, 2001

Current Position

Associate Professor of Pharmacology and Toxicology, Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, Kentucky
Lab website: http://louisville.edu/ocrp/matoba

Publications (as of January 2014)

Peer-reviewed publications

  1. Kessans, S. A., Linhart, M. D., Matoba, N.* and Mor, T.* (2013). Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed in Nicotiana benthamiana. Plant Biotechnol J 11(6): 681-690.
  2. Hamorsky, K. T., Grooms-Williams, T. W., Husk, A. S., Bennett, L. J., Palmer, K. E. and Matoba, N.* (2013). Efficient single tobamoviral vector-based bioproduction of broadly neutralizing anti-HIV-1 monoclonal antibody VRC01 in Nicotiana benthamiana plants and utility of VRC01 in combination microbicides. Antimicrob Agents Chemother 57(5): 2076-2086.
  3. Hamorsky, K. T., Kouokam, J. C., Bennett, L. J., Baldauf, K. J., Kajiura, H., Fujiyama, K. and Matoba, N.* (2013). Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks. PLoS Negl Trop Dis 7(3): e2046.
  4. MATSUDA, R., TAHARA, A., MATOBA, N. and FUJIWARA, K. (2012). Virus Vector-Mediated Rapid Protein Production in Nicotiana benthamiana: Effects of Temperature and Photosynthetic Photon Flux Density on Hemagglutinin Accumulation. Environment Control in Biology 50(4): 375-381.
  5. Kouokam, J. C., Huskens, D., Schols, D., Johannemann, A., Riedell, S. K., Walter, W., Walker, J. M., Matoba, N., O'Keefe, B. R. and Palmer, K. E. (2011). Investigation of griffithsin's interactions with human cells confirms its outstanding safety and efficacy profile as a microbicide candidate. PLoS One 6(8): e22635.
  6. Matoba, N.*, Shah, N. R. and Mor, T. S. (2011). Humoral immunogenicity of an HIV-1 envelope residue 649-684 membrane-proximal region peptide fused to the plague antigen F1-V. Vaccine 29(34): 5584-5590.
  7. Matoba, N.*, Davis, K. R. and Palmer, K. E. (2011). Recombinant protein expression in Nicotiana. Methods Mol Biol 701: 199-219.
  8. Matoba, N.*, Husk, A. S., Barnett, B. W., Pickel, M. M., Arntzen, C. J., Montefiori, D. C., Takahashi, A., Tanno, K., Omura, S., Cao, H., Mooney, J. P., Hanson, C. V. and Tanaka, H. (2010). HIV-1 neutralization profile and plant-based recombinant expression of actinohivin, an Env glycan-specific lectin devoid of T-cell mitogenic activity. PLoS One 5(6): e11143.
  9. Matoba, N., Kajiura, H., Cherni, I., Doran, J. D., Bomsel, M., Fujiyama, K. and Mor, T. S. (2009). Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR. Plant Biotechnol J 7(2): 129-145.
  10. Matoba, N., Griffin, T. A., Mittman, M., Doran, J. D., Alfsen, A., Montefiori, D. C., Hanson, C. V., Bomsel, M. and Mor, T. S. (2008). Transcytosis-blocking abs elicited by an oligomeric immunogen based on the membrane proximal region of HIV-1 gp41 target non-neutralizing epitopes. Curr HIV Res 6(3): 218-229.
  11. Matoba, N., Geyer, B. C., Kilbourne, J., Alfsen, A., Bomsel, M. and Mor, T. S. (2006). Humoral immune responses by prime-boost heterologous route immunizations with CTB-MPR(649-684), a mucosal subunit HIV/AIDS vaccine candidate. Vaccine 24(23): 5047-5055.
  12. Matoba, N., Magerus, A., Geyer, B. C., Zhang, Y., Muralidharan, M., Alfsen, A., Arntzen, C. J., Bomsel, M. and Mor, T. S. (2004). A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs. Proc Natl Acad Sci U S A 101(37): 13584-13589.
  13. Onishi, K., Matoba, N., Yamada, Y., Doyama, N., Maruyama, N., Utsumi, S. and Yoshikawa, M. (2004). Optimal designing of beta-conglycinin to genetically incorporate RPLKPW, a potent anti-hypertensive peptide. Peptides 25(1): 37-43.
  14. Matoba, N., Yamada, Y. and Yoshikawa, M. (2003). Design of a genetically modified soybean protein preventing hypertension based on an anti-hypertensive peptide derived from ovalbumin. Curr Med Chem Cardiovasc Hematol Agents 1(2): 197-202.
  15. Yamada, Y., Matoba, N., Usui, H., Onishi, K. and Yoshikawa, M. (2002). Design of a highly potent anti-hypertensive peptide based on ovokinin(2-7). Biosci Biotechnol Biochem 66(6): 1213-1217.
  16. Matoba, N., Doyama, N., Yamada, Y., Maruyama, N., Utsumi, S. and Yoshikawa, M. (2001). Design and production of genetically modified soybean protein with anti-hypertensive activity by incorporating potent analogue of ovokinin(2-7). FEBS Lett 497(1): 50-54.
  17. Matoba, N., Yamada, Y., Usui, H., Nakagiri, R. and Yoshikawa, M. (2001). Designing potent derivatives of ovokinin(2-7), an anti-hypertensive peptide derived from ovalbumin. Biosci Biotechnol Biochem 65(3): 736-739.
  18. Yoshikawa, M., Fujita, H., Matoba, N., Takenaka, Y., Yamamoto, T., Yamauchi, R., Tsuruki, H. and Takahata, K. (2000). Bioactive peptides derived from food proteins preventing lifestyle-related diseases. Biofactors 12(1-4): 143-146.
  19. Matoba, N., Usui, H., Fujita, H. and Yoshikawa, M. (1999). A novel anti-hypertensive peptide derived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Lett 452(3): 181-184.
Protocols by Nobuyuki Matoba
  1. Monoclonal Antibody Purification (Nicotiana benthamiana Plants)