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Elias K. Haddad

Education

Ph.D. in Immunology, Department of Microbiology & Immunology, McGill University, Montreal, Canada, 1997

Current position

Associate Member, Vaccine and Gene Therapy Institute Florida, USA

Publications (since 2010)

  1. Locci, M., Havenar-Daughton, C., Landais, E., Wu, J., Kroenke, M. A., Arlehamn, C. L., Su, L. F., Cubas, R., Davis, M. M., Sette, A., Haddad, E. K., Poignard, P. and Crotty, S. (2013). Human circulating PD-(+)1CXCR3(-)CXCR5(+) memory Tfh cells are highly functional and correlate with broadly neutralizing HIV antibody responses. Immunity 39(4): 758-769.
  2. Samuel, S., Beljanski, V., Van Grevenynghe, J., Richards, S., Ben Yebdri, F., He, Z., Nichols, C., Belgnaoui, S. M., Steel, C., Goulet, M. L., Shamy, A., Brown, D., Abesada, G., Haddad, E. K. and Hiscott, J. (2013). BCL-2 inhibitors sensitize therapy-resistant chronic lymphocytic leukemia cells to VSV oncolysis. Mol Ther 21(7): 1413-1423.
  3. Goulet, M. L., Olagnier, D., Xu, Z., Paz, S., Belgnaoui, S. M., Lafferty, E. I., Janelle, V., Arguello, M., Paquet, M., Ghneim, K., Richards, S., Smith, A., Wilkinson, P., Cameron, M., Kalinke, U., Qureshi, S., Lamarre, A., Haddad, E. K., Sekaly, R. P., Peri, S., Balachandran, S., Lin, R. and Hiscott, J. (2013). Systems analysis of a RIG-I agonist inducing broad spectrum inhibition of virus infectivity. PLoS Pathog 9(4): e1003298.
  4. Cubas, R. A., Mudd, J. C., Savoye, A. L., Perreau, M., van Grevenynghe, J., Metcalf, T., Connick, E., Meditz, A., Freeman, G. J., Abesada-Terk, G., Jr., Jacobson, J. M., Brooks, A. D., Crotty, S., Estes, J. D., Pantaleo, G., Lederman, M. M. and Haddad, E. K. (2013). Inadequate T follicular cell help impairs B cell immunity during HIV infection. Nat Med 19(4): 494-499.
  5. Mudd, J. C., Murphy, P., Manion, M., Debernardo, R., Hardacre, J., Ammori, J., Hardy, G. A., Harding, C. V., Mahabaleshwar, G. H., Jain, M. K., Jacobson, J. M., Brooks, A. D., Lewis, S., Schacker, T. W., Anderson, J., Haddad, E. K., Cubas, R. A., Rodriguez, B., Sieg, S. F. and Lederman, M. M. (2013). Impaired T-cell responses to sphingosine-1-phosphate in HIV-1 infected lymph nodes. Blood 121(15): 2914-2922.
  6. El-Far, M., Isabelle, C., Chomont, N., Bourbonniere, M., Fonseca, S., Ancuta, P., Peretz, Y., Chouikh, Y., Halwani, R., Schwartz, O., Madrenas, J., Freeman, G. J., Routy, J. P., Haddad, E. K. and Sekaly, R. P. (2013). Down-regulation of CTLA-4 by HIV-1 Nef protein. PLoS One 8(1): e54295.
  7. Vaccari, M., Halwani, R., Patterson, L. J., Boasso, A., Beal, J., Tryniszewska, E., Hryniewicz, A., Venzon, D., Haddad, E. K., El-Far, M., Rosati, M., Pavlakis, G. N., Felber, B. K., Al-Muhsen, S., Robert-Guroff, M., Sekaly, R. P. and Franchini, G. (2013). Antibodies to gp120 and PD-1 expression on virus-specific CD8+ T cells in protection from simian AIDS. J Virol 87(6): 3526-3537.
  8. Klatt, N. R., Canary, L. A., Sun, X., Vinton, C. L., Funderburg, N. T., Morcock, D. R., Quinones, M., Deming, C. B., Perkins, M., Hazuda, D. J., Miller, M. D., Lederman, M. M., Segre, J. A., Lifson, J. D., Haddad, E. K., Estes, J. D. and Brenchley, J. M. (2013). Probiotic/prebiotic supplementation of antiretrovirals improves gastrointestinal immunity in SIV-infected macaques. J Clin Invest 123(2): 903-907.
  9. Tzelepis, F., Joseph, J., Haddad, E. K., Maclean, S., Dudani, R., Agenes, F., Peng, S. L., Sekaly, R. P. and Sad, S. (2013). Intrinsic role of FoxO3a in the development of CD8+ T cell memory. J Immunol 190(3): 1066-1075.
  10. Perreau, M., Savoye, A. L., De Crignis, E., Corpataux, J. M., Cubas, R., Haddad, E. K., De Leval, L., Graziosi, C. and Pantaleo, G. (2013). Follicular helper T cells serve as the major CD4 T cell compartment for HIV-1 infection, replication, and production. J Exp Med 210(1): 143-156.
  11. Trautmann, L., Mbitikon-Kobo, F. M., Goulet, J. P., Peretz, Y., Shi, Y., Van Grevenynghe, J., Procopio, F. A., Boulassel, M. R., Routy, J. P., Chomont, N., Haddad, E. K. and Sekaly, R. P. (2012). Profound metabolic, functional, and cytolytic differences characterize HIV-specific CD8 T cells in primary and chronic HIV infection. Blood 120(17): 3466-3477.
  12. Petrovas, C., Yamamoto, T., Gerner, M. Y., Boswell, K. L., Wloka, K., Smith, E. C., Ambrozak, D. R., Sandler, N. G., Timmer, K. J., Sun, X., Pan, L., Poholek, A., Rao, S. S., Brenchley, J. M., Alam, S. M., Tomaras, G. D., Roederer, M., Douek, D. C., Seder, R. A., Germain, R. N., Haddad, E. K. and Koup, R. A. (2012). CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest 122(9): 3281-3294.
  13. Peretz, Y., He, Z., Shi, Y., Yassine-Diab, B., Goulet, J. P., Bordi, R., Filali-Mouhim, A., Loubert, J. B., El-Far, M., Dupuy, F. P., Boulassel, M. R., Tremblay, C., Routy, J. P., Bernard, N., Balderas, R., Haddad, E. K. and Sekaly, R. P. (2012). CD160 and PD-1 co-expression on HIV-specific CD8 T cells defines a subset with advanced dysfunction. PLoS Pathog 8(8): e1002840.
  14. Haddad, E. K. and Hiscott, J. (2012). Cytokine networks during HIV infection: shifting the balance. Cytokine Growth Factor Rev 23(4-5): 139-141.
  15. van Grevenynghe, J., Cubas, R. A., DaFonseca, S., Metcalf, T., Tremblay, C. L., Trautmann, L., Sekaly, R. P., Schatzle, J. and Haddad, E. K. (2012). Foxo3a: an integrator of immune dysfunction during HIV infection. Cytokine Growth Factor Rev 23(4-5): 215-221.
  16. Klatt, N. R., Estes, J. D., Sun, X., Ortiz, A. M., Barber, J. S., Harris, L. D., Cervasi, B., Yokomizo, L. K., Pan, L., Vinton, C. L., Tabb, B., Canary, L. A., Dang, Q., Hirsch, V. M., Alter, G., Belkaid, Y., Lifson, J. D., Silvestri, G., Milner, J. D., Paiardini, M., Haddad, E. K. and Brenchley, J. M. (2012). Loss of mucosal CD103+ DCs and IL-17+ and IL-22+ lymphocytes is associated with mucosal damage in SIV infection. Mucosal Immunol 5(6): 646-657.
  17. Johnson, M. J., Petrovas, C., Yamamoto, T., Lindsay, R. W., Lore, K., Gall, J. G., Gostick, E., Lefebvre, F., Cameron, M. J., Price, D. A., Haddad, E., Sekaly, R. P., Seder, R. A. and Koup, R. A. (2012). Type I IFN induced by adenovirus serotypes 28 and 35 has multiple effects on T cell immunogenicity. J Immunol 188(12): 6109-6118.
  18. Gomez, C. E., Perdiguero, B., Jimenez, V., Filali-Mouhim, A., Ghneim, K., Haddad, E. K., Quakkelaar, E. D., Delaloye, J., Harari, A., Roger, T., Duhen, T., Sekaly, R. P., Melief, C. J., Calandra, T., Sallusto, F., Lanzavecchia, A., Wagner, R., Pantaleo, G. and Esteban, M. (2012). Systems analysis of MVA-C induced immune response reveals its significance as a vaccine candidate against HIV/AIDS of clade C. PLoS One 7(4): e35485.
  19. Kroenke, M. A., Eto, D., Locci, M., Cho, M., Davidson, T., Haddad, E. K. and Crotty, S. (2012). Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation. J Immunol 188(8): 3734-3744.
  20. Yamamoto, T., Johnson, M. J., Price, D. A., Wolinsky, D. I., Almeida, J. R., Petrovas, C., Nason, M., Yeh, W. W., Shen, L., Roederer, M., Rao, S. S., McDermott, A. B., Lefebvre, F., Nabel, G. J., Haddad, E. K., Letvin, N. L., Douek, D. C. and Koup, R. A. (2012). Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infection. J Virol 86(10): 5877-5884.
  21. Haddad, E. K. and Pantaleo, G. (2012). Systems biology in the development of HIV vaccines. Curr Opin HIV AIDS 7(1): 44-49.
  22. Kibler, K. V., Gomez, C. E., Perdiguero, B., Wong, S., Huynh, T., Holechek, S., Arndt, W., Jimenez, V., Gonzalez-Sanz, R., Denzler, K., Haddad, E. K., Wagner, R., Sekaly, R. P., Tartaglia, J., Pantaleo, G., Jacobs, B. L. and Esteban, M. (2011). Improved NYVAC-based vaccine vectors. PLoS One 6(11): e25674.
  23. Caskey, M., Lefebvre, F., Filali-Mouhim, A., Cameron, M. J., Goulet, J. P., Haddad, E. K., Breton, G., Trumpfheller, C., Pollak, S., Shimeliovich, I., Duque-Alarcon, A., Pan, L., Nelkenbaum, A., Salazar, A. M., Schlesinger, S. J., Steinman, R. M. and Sekaly, R. P. (2011). Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans. J Exp Med 208(12): 2357-2366.
  24. van Grevenynghe, J., Cubas, R. A., Noto, A., DaFonseca, S., He, Z., Peretz, Y., Filali-Mouhim, A., Dupuy, F. P., Procopio, F. A., Chomont, N., Balderas, R. S., Said, E. A., Boulassel, M. R., Tremblay, C. L., Routy, J. P., Sekaly, R. P. and Haddad, E. K. (2011). Loss of memory B cells during chronic HIV infection is driven by Foxo3a- and TRAIL-mediated apoptosis. J Clin Invest 121(10): 3877-3888.
  25. Quakkelaar, E. D., Redeker, A., Haddad, E. K., Harari, A., McCaughey, S. M., Duhen, T., Filali-Mouhim, A., Goulet, J. P., Loof, N. M., Ossendorp, F., Perdiguero, B., Heinen, P., Gomez, C. E., Kibler, K. V., Koelle, D. M., Sekaly, R. P., Sallusto, F., Lanzavecchia, A., Pantaleo, G., Esteban, M., Tartaglia, J., Jacobs, B. L. and Melief, C. J. (2011). Improved innate and adaptive immunostimulation by genetically modified HIV-1 protein expressing NYVAC vectors. PLoS One 6(2): e16819.
  26. Flatz, L., Roychoudhuri, R., Honda, M., Filali-Mouhim, A., Goulet, J. P., Kettaf, N., Lin, M., Roederer, M., Haddad, E. K., Sekaly, R. P. and Nabel, G. J. (2011). Single-cell gene-expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines. Proc Natl Acad Sci U S A 108(14): 5724-5729.
  27. Cameron, P. U., Saleh, S., Sallmann, G., Solomon, A., Wightman, F., Evans, V. A., Boucher, G., Haddad, E. K., Sekaly, R. P., Harman, A. N., Anderson, J. L., Jones, K. L., Mak, J., Cunningham, A. L., Jaworowski, A. and Lewin, S. R. (2010). Establishment of HIV-1 latency in resting CD4+ T cells depends on chemokine-induced changes in the actin cytoskeleton. Proc Natl Acad Sci U S A 107(39): 16934-16939.
  28. Said, E. A., Dupuy, F. P., Trautmann, L., Zhang, Y., Shi, Y., El-Far, M., Hill, B. J., Noto, A., Ancuta, P., Peretz, Y., Fonseca, S. G., Van Grevenynghe, J., Boulassel, M. R., Bruneau, J., Shoukry, N. H., Routy, J. P., Douek, D. C., Haddad, E. K. and Sekaly, R. P. (2010). Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection. Nat Med 16(4): 452-459.
Protocols by Elias K. Haddad
  1. T Follicular Helper Cell Coculture Assay