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Demosthenis Chronis


Ph.D. in Agronomy, Division of Plant Science, University of Missouri, Columbia, MO, USA, 2006

Current Position

Research Molecular Biologist in Robert W. Holley Center, USDA-ARS at Ithaca NY


  1. Yang, Y., Jittayasothorn, Y., Chronis, D., Wang, X., Cousins, P. and Zhong, G. Y. (2013). Molecular characteristics and efficacy of 16D10 siRNAs in inhibiting root-knot nematode infection in transgenic grape hairy roots. PLoS One 8(7): e69463.
  2. Chen, S., Chronis, D. and Wang, X. (2013). The novel GrCEP12 peptide from the plant-parasitic nematode Globodera rostochiensis suppresses flg22-mediated PTI. Plant Signal Behav 8(9).
  3. Chronis, D., Chen, S., Lu, S., Hewezi, T., Carpenter, S. C., Loria, R., Baum, T. J. and Wang, X. (2013). A ubiquitin carboxyl extension protein secreted from a plant-parasitic nematode Globodera rostochiensis is cleaved in planta to promote plant parasitism. Plant J 74(2): 185-196.
  4. Kim, W. S., Chronis, D., Juergens, M., Schroeder, A. C., Hyun, S. W., Jez, J. M. and Krishnan, H. B. (2012). Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman-Birk protease inhibitor in seeds. Planta 235(1): 13-23.
  5. Lee, C., Chronis, D., Kenning, C., Peret, B., Hewezi, T., Davis, E. L., Baum, T. J., Hussey, R., Bennett, M. and Mitchum, M. G. (2011). The novel cyst nematode effector protein 19C07 interacts with the Arabidopsis auxin influx transporter LAX3 to control feeding site development. Plant Physiol 155(2): 866-880.
  6. Yu, H., Chronis, D., Lu, S. and Wang, X. (2011). Chorismate mutase: an alternatively spliced parasitism gene and a diagnostic marker for three important Globodera nematode species. European Journal of Plant Pathology 129(1): 89-102.
  7. Lu, S. W., Chen, S., Wang, J., Yu, H., Chronis, D., Mitchum, M. G. and Wang, X. (2009). Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from the potato cyst nematode Globodera rostochiensis. Mol Plant Microbe Interact 22(9): 1128-1142.
  8. Krishnan, H. B. and Chronis, D. (2008). Functional nodFE genes are present in Sinorhizobium sp. strain MUS10, a symbiont of the tropical legume Sesbania rostrata. Appl Environ Microbiol 74(9): 2921-2923.
  9. Lorio, J. C., Chronis, D. and Krishnan, H. B. (2006). Y4xP, an open reading frame located in a type III protein secretion system locus of Sinorhizobium fredii USDA257 and USDA191, encodes cysteine synthase. Mol Plant Microbe Interact 19(6): 635-643.
  10. Chronis, D. and Krishnan, H. B. (2004). Sulfur assimilation in soybean (Glycine max [L.] Merr.): molecular cloning and characterization of a cytosolic isoform of serine acetyltransferase. Planta 218(3): 417-426.
  11. Chronis, D. and Krishnan, H. B. (2003). Sulfur assimilation in soybean (Glycine max [L.] Merr.): Molecular cloning and characterization of O-acetylserine (thiol) lyase (cysteine synthase). Crop Sci 43: 1819-1827.
Protocols by Demosthenis Chronis
  1. Potato Transformation
  2. In vitro Nematode Infection on Potato Plant