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David Moraga Amador

Education

Ph.D. in Biochemistry, Department of Chemistry, University of Nebraska, Lincoln, NE, 1986

Current position

Project Manager, Next Generation Sequencing, Interdisciplinary Center for Biotechnology, Uni-versity of Florida (1993-present)
Academic and professional interests: Biotechnology/ Molecular Biology Applications in Develop-ing Nations; Next Generation Sequencing Technologies

Publication

Refereed journals

  1. Wang, Y., An, C., Zhang, X., Yao, J., Zhang, Y., Sun, Y., Yu, F., Amador, D. M. and Mou, Z. (2013). The Arabidopsis elongator complex subunit2 epigenetically regulates plant immune re-sponses. Plant Cell 25(2): 762-776.
  2. Yongsheng Wang, Christopher T DeFraia, Chuanfu An, Xudong Zhang, Jiqiang Yao, Yanping Zhang, Yijun Sun, Fahong Yu, David M Amador, Zhonglin Mou (2012). Elongator Epigenetically Regulates Immune Responses in Arabidopsis thaliana. Submitted for publication in PLoS Genet-ics (July 2012).
  3. Campos, G. S., Moreau, V. H., Bandeira, A., Barberino, G., Almeida, P. F., Amador, D. M., de Lima, M. O. and Sardi, S. I. (2008). Molecular detection and genetic diversity of norovirus in hospitalized young adults with acute gastroenteritis in Bahia, Brazil. Arch Virol 153(6): 1125-1129.
  4. Dhekney, S., Litz, R., Amador, D. M. and Yadav, A. (2007). Potential for introducing cold toler-ance into papaya by transformation with C-repeat binding factor (CBF) genes. In Vitro Cellular & Developmental Biology-Plant 43(3): 195-202.
  5. Garcia-Rodriguez, A. I., Moraga-Amador, D., Farmerie, W., McGuire, P. and King, T. L. (2000). Isolation and characterization of microsatellite DNA markers in the Florida manatee (Trichechus manatus latirostris) and their application in selected Sirenian species. Mol Ecol 9(12): 2161-2163.
  6. Hutson, R. G., Kitoh, T., Moraga Amador, D. A., Cosic, S., Schuster, S. M. and Kilberg, M. S. (1997). Amino acid control of asparagine synthetase: relation to asparaginase resistance in hu-man leukemia cells. Am J Physiol 272(5 Pt 1): C1691-1699.
  7. Sheng, S., Moraga-Amador, D. A., van Heeke, G., Allison, R. D., Richards, N. G. and Schuster, S. M. (1993). Glutamine inhibits the ammonia-dependent activities of two Cys-1 mutants of hu-man asparagine synthetase through the formation of an abortive complex. J Biol Chem 268(22): 16771-16780.
  8. Martin, J. K., Sun, W., Moraga, A. D., Schuster, S. M. and Wylie, D. E. (1993). An investigation into the mechanism ofL-asparaginase resistance in L5178Y murine leukemia cells. Amino Acids 5(1): 51-69.
  9. Sheng, S., Moraga, D. A., Van Heeke, G. and Schuster, S. M. (1992). High-level expression of human asparagine synthetase and production of monoclonal antibodies for enzyme purification. Protein Expr Purif 3(4): 337-346.
  10. Ibrahim, M. S., Tamashiro, W. K., Moraga, D. A. and Scott, A. L. (1989). Antigen shedding from the surface of the infective stage larvae of Dirofilaria immitis. Parasitology 99 Pt 1: 89-97.
  11. Moraga-Amador, D. A., MacPhee-Quiggley, K. M., Keefer, J. F. and Schuster, S. M. (1989). As-paragine catabolism in rat liver mitochondria. Arch Biochem Biophys 268(1): 314-326.
  12. Tamashiro, W. K., Ibrahim, M. S., Moraga, D. A. and Scott, A. L. (1989). Dirofilaria immitis: studies on anti-microfilarial immunity in Lewis rats. Am J Trop Med Hyg 40(4): 368-376.
  13. Scott, A. L., Diala, C., Moraga, D. A., Ibrahim, M. S., Redding, L. and Tamashiro, W. K. (1988). Dirofilaria immitis: biochemical and immunological characterization of the surface antigens from adult parasites. Exp Parasitol 67(2): 307-323.
  14. Keefer, J. F., Moraga, D. A. and Schuster, S. M. (1985). Comparison of glycine metabolism in mouse lymphoma cells either sensitive or resistant to L-asparaginase. Biochem Pharmacol 34(4): 559-565.
  15. Keefer, J. F., Moraga, D. A. and Schuster, S. M. (1985). Amino acid content of L5178Y and L5178Y/L-ASE cells after L-asparaginase treatment. Biochem Med 34(2): 135-150.
  16. Unnithan, S., Moraga, D. A. and Schuster, S. M. (1984). A high-performance liquid chromatog-raphy assay for asparagine synthetase. Anal Biochem 136(1): 195-201.

Laboratory manuals

  1. Molecular Techniques: Protein Chemistry and Molecular Cloning. 2004. David Moraga Amador, Mick Popp, Nancy Denslow and Bill Farmerie. ICBR- Education and Training Core Laboratory, Uni-versity of Florida.
  2. AFLP Workshop. 2002. David Moraga Amador, Daniel Brazeau, Bill Farmerie, Allison Blake, Ginger Clark, and Mark Whitten. ICBR- Education and Training Core Laboratory, University of Florida.
  3. Proteomics: Analysis of Gene Products. 2002. David Moraga Amador. ICBR- Education and Training Core Laboratory, University of Florida.
  4. Recombinant DNA Analysis. 2002. David Moraga Amador and Sharon Norton. ICBR- Education and Training Core Laboratory, University of Florida.
  5. Antibody Applications. 2002. Linda Green, Neal Benson, Greg Erdos, and David Moraga Amador. ICBR- Education and Training Core Laboratory, University of Florida.
  6. Molecular Markers: Tools for Developing Microsatellites. 2002. David Moraga Amador, Sharon Nor-ton and Ginger Clark. ICBR- Education and Training Core Laboratory, University of Florida.
  7. Microarray Gene Expression Analysis. 2002. David Moraga Amador, Sharon Norton and Mick Popp. ICBR- Education and Training Core Laboratory, University of Florida.
  8. Diagnóstico Molecular del Virus Tristeza y Algunos Miembros del Subgrupo Begomovirus. 2000. David Moraga Amador and Bettina Moser. ICBR- Education and Training Core Laboratory, Uni-versity of Florida.
  9. Molecular Cloning Techniques: Isolation of Plant Genes. 2001. David Moraga Amador, Carla Lyerly, David Clark, Angie Dobberfuhl and Bill Farmerie. ICBR- Education and Training Core Laboratory, University of Florida.
  10. Glycoanalysis of Proteins. 2000. Michael Horst and David Moraga Amador.
  11. In Situ Hybridization RT-PCR. 2000. David Moraga Amador. ICBR- Education and Training Core Laboratory, University of Florida.
  12. Molecular Biology and Gene Manipulation Laboratory Manual. 1998. Lekan M. Latinwo, Caroline Fasanya-Odewumi, David Moraga Amador and Bill Farmerie. Paradigm Custom Publishing Inc. ISBN 1-58175-012-9
Protocols by David Moraga Amador
  1. Library Construction for Genome-wide Bisulfite Sequencing in Plants